PIONEERS OF MICROBIOLOGY
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Transcript of PIONEERS OF MICROBIOLOGY
PIONEERS OF MICROBIOLOGY
Prepared by: Ms.Irish M. Sequihod
INTRODUCTION Bacteria and protozoa – were
the first microorganisms observed by humans
It took 200 years before man was able to establish a connection between microorganism and infectious disease.
It was the discovery of microscope that made microbiology understand more about the nature of diseases.
IMPORTANT PERSONS Anton van Leeuwenhoek –
was the first person to see live bacteria and protozoa
Father of microbiology A fabric merchant, surveyor,
wine assayer, and a minor city official in Delf, Holland
Ground tiny glass lenses mounted in metal frames
Single lens microscopes “Animalcules”
LEEUWENHOEK Scrapings from teeth Ditches and pond water Blood and sperm Diarrheal stool
LOUIS PASTEUR A French chemist Fermentation Grapes (glucose)-yeasts-ethanol Disproved the spontaneous
generation Aerobes and anaerobes Developed the process
pasteurization to kill microbes that were causing to spoil. Can be used to kill pathogens in many types of liquids.
Involves heating to 55 degree celsius and holding at that temperature for several minutes.
LOUIS PASTEUR He discovered the infectious agents that
caused the silkworm diseases that were crippling the silk industry in France.
Germ theory of disease- made contributions that a certain microorganism can cause a certain disease.
Hospital practices to minimize the spread of disease by pathogens
Developed vaccines to prevent chicken cholera, anthrax, and swine erysipelas
Developed the vaccine to prevent rabies in dogs and successfully used the vacine to treat human rabies
ROBERT KOCH German physician Contributor to the germ
theory of disease He proved that anthrax
bacillus was truly the cause of anthrax.
Koch’s postulate Discovered that a. bacillus
produces spores capable of resisting adverse conditions
Fixing and staining and photographing bacteria
ROBERT KOCH Developed methods of cultivating
bacteria on solid media. R.J. Petri- colleague of robert Frua hess- agar (polysaccharide)as
solidifying agent Dicovered mycobacterium tuberculosis
and vibrio cholera
ABIOGENESIS Spontaneous
generation – abiogenesis
Classical notions of abiogenesis, now more precisely known as spontaneous generation, held that certain complex, living organisms are generated by decaying organic substances.
BIOGENESIS John tyndall and Louis Pasteur – were the
one who disproved the abiogenesis theory. Rudolf Virchow – was the first who
proposed about biogenesis
KOCH’S POSTULATE A microorganism must be found in all cases
of the disease and must not be present in healthy animals or humans.
The microorganism must be isolated from the diseased animal or human and grown in pure culture in the laboratory.
The same disease must be produced when microorganisms from the pure culture are inoculated into healthy susceptible laboratory animals
The same microorganism must be recovered from the experimentaly infected animals and grown again in pure culture
KOCH’S POSTULATE Exeptions
have to grow the pathogen in a media but not all can grow (viruses, rickettsias
Some pathogens are species-specific
Synergistics –caused by more than one organism
Less pathogenic when cultured
THE GOLDEN AGE OF MICROBIOLOGY In the late 1800s and for the first decade of
the 1900s, scientists seized the opportunity to further develop the germ theory of disease as enunciated by Pasteur and proved by Koch.
There emerged a Golden Age of Microbiology during which many agents of different infectious diseases were identified. Many of the etiologic agents of microbial disease were discovered during that period, leading to the ability to halt epidemics by interrupting the spread of microorganisms.
THE GOLDEN AGE OF MICROBIOLOGY Despite the advances in microbiology, it
was rarely possible to render life-saving therapy to an infected patient. Then, after World War II, the antibiotics were introduced to medicine. The incidence of pneumonia, tuberculosis, meningitis, syphilis, and many other diseases declined with the use of antibiotics.
THE GOLDEN AGE OF MICROBIOLOGY Work with viruses could not be effectively
performed until instruments were developed to help scientists see these disease agents. In the 1940s, the electron microscope was developed and perfected. In that decade, cultivation methods for viruses were also introduced, and the knowledge of viruses developed rapidly.
With the development of vaccines in the 1950s and 1960s, such viral diseases as polio, measles, mumps, and rubella came under control.
THE MODERN AGE OF MICROBIOLOGY Modern microbiology reaches into many fields
of human endeavor, including the development of pharmaceutical products, the use of quality-control methods in food and dairy product production, the control of disease-causing microorganisms in consumable waters, and the industrial applications of microorganisms.
Microorganisms are used to produce vitamins, amino acids, enzymes, and growth supplements. They manufacture many foods, including fermented dairy products, as well as other fermented foods such as pickles, sauerkraut, breads, and alcoholic beverages.