PigmentPigment Why does this bacteria change color?

PigmentWhy does this bacteria change color?

Garcia, AngelaUrbina, Maria

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Who?

• Angela Garcia (right)• Maria Urbina (left)

• Northeast Magnet High School

• Bio-Research Club 2006-2007

• Juniors


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Experiments• Experiment # 1 Salinity Concentration

– To what extent does the salt concentration affect marinococcus coloration?

• Experiment # 2 Darkness– To what extent does the light in the room affect

marinococcus coloration?• Experiment # 3 Temperature (°C)

– To what extent does the temperature affect marinococcus coloration?

• Experiment # 4 pH level– To what extent does the pH level affect marinococcus

coloration?


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Marinococcus• Flavin pigment

– (natural yellow pigment)

• Halophilic – (grows only in salt rich environment)

• Gram positive cocci – (Spherical, spiral, or curved shaped bacteria

that stained purple/blue during iodine staining)


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Items being tested• Marinococcus

– # 31 (light yellow)– # 32 (bright orange)

• Rate at which Growth can be Achieved – 1% - 20% Salt Content– 4° - 50° C– pH 9 - pH 11

• Control– 37°C– pH 9– 10% Salt Concentration


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Marinococcus

Our bacteria, here you can see the difference in the colors.


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Ex: 1 Salinity Concentration

• To want extent does salinity concentration affect coloration of marinococcus? – H1 If salt content is increased then the

coloration of marinococcus will be affected.

– H0 If salt content is increased then the coloration of marinococcus will not be affected.

– H2 If salt content is decreased then the coloration of marinococcus will be affected.


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The Test• Testing

– Minimum 1% salinity – 5% salinity– Optimum 10% salinity– Maximum 20% salinity

• Control– Temperature - 37° C– pH level - 9

• Amount Tested– 24 petri dishes will be set up. 12 for each type, #31 &

#32, of the bacteria.


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Preliminary Data

• 2-8-07– Started Colonies

on 1%,5%,10%, and 20% agar plates. Also began new colonies and placed the older ones in freezer to prevent further growth.


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Collecting bacteria from the original slants.

Proper technique for creating individual colonies.

Preparing to flame loop for transfer.

Fun in the lab.


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First Week Results

• 2-15-07 • #31

– 1% - lead sized individual colonies with diminutive, small, growth

– 5% - lumped colonies in a strip line but no individual growth

• 2-15-07• #32

– 1% - diminutive growth better than the 20%

– 5% - an abundant of tiny colonies sparsely spread


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• 2-15-07• #31

– 10% - As expected, colonies grew quite well, many useable individual colonies

– 20% - One mass colony, most likely the first streak when transferred, no individual colonies

• 2-15-07• #32

– 10% - As expected, colonies grew quite well, many useable individual colonies

– 20% - Two mass colonies, most likely the first streak when transferred, no individual colonies

First Week Results


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Other Notes• 2-15-07

– The temperature had settled to 30°C on 2-15-07 so we moved it up to the control 37°C.

– Conclusion after first week• The salinity concentration has no affect on

coloration, just on the growth of marinococcus.


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Images• 2-21-07

• 2-22-07


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Images• 2-21-07


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Images• 2-21-07


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Images• 2-21-07


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Other Notes• 2-21-07

– Temperature was back at 30°C, moved up to the control

– No useable image of 1% #31 was taken so 2-22-07 another picture will be collected

– Agar has dried out, crystals have formed on most plates


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Ex : 2 Darkness

• To want extent does the light in the room affect coloration of marinococcus? – H1 If colonies of the marinococcus are kept in

the dark then coloration will be affected.

– H0 If colonies of the marinococcus are kept in the dark then coloration will not be affected.

– H2 If colonies of the marinococcus are kept in the light then coloration will not be affected.


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The Test• Testing

– Dark (dark box)– Light (Florescent light)

• Control– Salt Concentration - 10%– Temperature - 37° C– pH level - 9

• Amount Tested– 6 petri dishes will be set up. 3 for each type, #31 &

#32, of the bacteria.

PigmentPigment Why does this bacteria change color?

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