PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat...

21
PHYSICAL METHODS FOR KILLING MICROBES • Sterilization methods – Incineration – Autoclave – Dry heat oven – Filter sterilization – Ultra violet light – Gamma radiation

TAGS:

Transcript of PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat...

Page 1: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

PHYSICAL METHODS FOR KILLING MICROBES

• Sterilization methods– Incineration– Autoclave– Dry heat oven – Filter sterilization– Ultra violet light– Gamma radiation

Page 2: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

The direct flame represents a rapid sterilization method

Incineration

Page 3: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

The autoclave uses moist heat and pressure

Page 4: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

Boiling water involvesmoist heat

• This is not a reliable method for sterilization unless tyndallization is used.

• Why not?

Page 5: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

Pasteurization does not sterilize

• It is used to kill unwanted or harmful microbes

• Why doesn’t it sterilize?

Page 6: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

Hot-air (dry-heat) oven

Time and Temperature: 338F for 11/2 hours

Page 7: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

Filter Sterilization

Page 8: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

Filtration traps microorganisms

Page 9: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

Ultraviolet light can be used to control microbial growth

UV light attacks the cell’s DNA

What are some disadvantages in the use of UV for sterilization?

Page 10: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

The Ionizing and Electromagnetic Spectrum of Energies

Page 11: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

Thymine Dimers of DNA form when exposed to UV light

Page 12: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

Thymine Dimers

Page 13: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

CHECK MOLES FOR THESE CHANGES

Page 14: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

GAMMA RADIATION

A widely used method for sterilizing materials that would be adversely

altered by heat (plastics, lensed equip. heart-lung machines etc).

Page 15: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

Logo for gamma radiationLook for this logo on those foods that have been treated with gamma

radiation, such as meats, and produce.

Page 16: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

PRESERVATION METHODS

Drying (dehydration) Method

Salting Method

Low pH Method

Do these methods sterilize the food? Explain.

Page 17: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

General Principles ofChemical Control

Page 18: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

Resistance of microbes to chemical agents

Page 19: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

Disinfectants

Page 20: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

Killing Action of certain metals

Page 21: PHYSICAL METHODS FOR KILLING MICROBES Sterilization methods –Incineration –Autoclave –Dry heat oven –Filter sterilization –Ultra violet light –Gamma radiation.

Killing Action of Some Antiseptics


Microbiology - Palamuru University · Sterilization and disinfection techniques. Principles and methods of sterilization. Physical methods-Autoclave, Hot air oven, pressure cooker,

Microbiology - Palamuru University · Sterilization and disinfection techniques. Principles and methods of sterilization. Physical methods-Autoclave, Hot air oven, pressure cooker,

Correction: Overview of sterilization methods for UHMWPE ...

Correction: Overview of sterilization methods for UHMWPE ...

Sterilization Methods and the Comparison of E-Beam Sterilization with Gamma … Vs... · 2018. 5. 29. · Although gamma irradiation has been used for many years in sterilization

Sterilization Methods and the Comparison of E-Beam Sterilization with Gamma … Vs... · 2018. 5. 29. · Although gamma irradiation has been used for many years in sterilization

LyondellBasell Industries | LyondellBasell - Sterilization of ......Vapor (steam) sterilization using an autoclave is one of the most widely used methods for heat sterilization of

LyondellBasell Industries | LyondellBasell - Sterilization of ......Vapor (steam) sterilization using an autoclave is one of the most widely used methods for heat sterilization of

Sterilization Methods and the Comparison of E-Beam Sterilization ...

Sterilization Methods and the Comparison of E-Beam Sterilization ...

Comparison of Different Low Temperature Sterilization Methods

Comparison of Different Low Temperature Sterilization Methods

DENT 1260 IC Unit 5 Unit 5 Sterilization 1. 3 methods of HEAT Sterilization Autoclave- steam under pressure Chemclave- chemical heated under pressure.

DENT 1260 IC Unit 5 Unit 5 Sterilization 1. 3 methods of HEAT Sterilization Autoclave- steam under pressure Chemclave- chemical heated under pressure.

Sterilization & Disinfection - Dentalytec...Sterilization and Disinfection Methods in Dentistry One of the primary responsibilities of dental healthcare professionals is to create

Sterilization & Disinfection - Dentalytec...Sterilization and Disinfection Methods in Dentistry One of the primary responsibilities of dental healthcare professionals is to create

Overview of sterilization methods for UHMWPE through ...

Overview of sterilization methods for UHMWPE through ...

Revised Sterilization Methods and Principles

Revised Sterilization Methods and Principles

INFECTION CONTROL and STERILIZATION UNIT - … · INFECTION CONTROL and STERILIZATION UNIT ... sterile medical devices Principles Methods Agents Limitations ... Asepsis, cleaning

INFECTION CONTROL and STERILIZATION UNIT - … · INFECTION CONTROL and STERILIZATION UNIT ... sterile medical devices Principles Methods Agents Limitations ... Asepsis, cleaning

Prepared media plate sterilization methods

Prepared media plate sterilization methods

Incineration - Frank's Hospital Workshopfrankshospitalworkshop.com/.../wikipedia/Incineration.pdfIncineration 1 Incineration Spittelau incineration plant in Vienna. Incineration is

Incineration - Frank's Hospital Workshopfrankshospitalworkshop.com/.../wikipedia/Incineration.pdfIncineration 1 Incineration Spittelau incineration plant in Vienna. Incineration is

THE CONSTRAINTS OF LEGACY STERILIZATION METHODS T€¦ · sterilization methods impose serious limitations in terms of efficacy, quality, or production processes of many of the newer

THE CONSTRAINTS OF LEGACY STERILIZATION METHODS T€¦ · sterilization methods impose serious limitations in terms of efficacy, quality, or production processes of many of the newer

Sterilization of health care products — Microbiological methods...©ISO 2018 Sterilization of health care products — Microbiological methods — Part 1: Determination of a population

Sterilization of health care products — Microbiological methods...©ISO 2018 Sterilization of health care products — Microbiological methods — Part 1: Determination of a population

The surgical domain · STERILIZATION Failure of normal methods of sterilization • Failure of an autoclave or power supply may suddenly interrupt normal sterilization procedures.

The surgical domain · STERILIZATION Failure of normal methods of sterilization • Failure of an autoclave or power supply may suddenly interrupt normal sterilization procedures.

Guidelines for cleaning, disinfection, and sterilization ... · 2. Cleaning, Disinfection, Sterilization This section describes the methods for cleaning, disinfection, and sterilization.

Guidelines for cleaning, disinfection, and sterilization ... · 2. Cleaning, Disinfection, Sterilization This section describes the methods for cleaning, disinfection, and sterilization.

Sterilization Methods and the Comparison of E-Beam ... · Advantages and disadvantages of sterilization methods (2-12). STERILIZATION METHOD ADVANTAGES DISADVANTAGES Dry heat sterilization

Sterilization Methods and the Comparison of E-Beam ... · Advantages and disadvantages of sterilization methods (2-12). STERILIZATION METHOD ADVANTAGES DISADVANTAGES Dry heat sterilization

Effects and efficacy of different sterilization and disinfection ... · Web viewSupporting information for Effects and efficacy of different sterilization and disinfection methods

Effects and efficacy of different sterilization and disinfection ... · Web viewSupporting information for Effects and efficacy of different sterilization and disinfection methods

Terminal Methods (Sterilization) [Autosaved]

Terminal Methods (Sterilization) [Autosaved]