Photofabrication of 3D Protein Hydrogels for Nanobiotechnology Applications
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Photofabrication of 3D Protein Hydrogels for Nanobiotechnology Applications
BAYINDIR GROUP JOURNAL CLUB-1
Erol Özgür
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MOTIVATIONS
• Microfabricated platforms in cellular biology: valuable research tools
• Microfluidics technology• Major problem is biocompatibility• Limited functionality of interfacial materials• No perturbation during experiment process is possible
• Aim: Platforms for cellular research that could be modified in situ
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Protein Cross-linking for Formation of 3D Hydrogels
• BSA (Bovine Serum Albumin) is a soluble protein with 607 residues
• BSA(aq) BSA(prec.)
• UV light: Inefficient, little control on the shape
• Multiphoton excitation: Precisely controlled submicrometer sized shapes
• Photosensitizers increase the efficiency of the process
• Optical parameters:Tsunami Ti:Sapphire laser at 730-740 nm, Zeiss Axiovert IM 100X Fluar 1.3 NA objective, with a confocal scanner
hν
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In Situ Formation of Protein Hydrogel
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3D Protein Hydrogels Created by Photofabrication with Photomasks
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Chemical Response of Protein Hydrogels: Na2SO4
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Chemical Response of Protein Hydrogels: Na2SO4
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Chemical Response of Protein Hydrogels: pH from 7 to 12.2 with NaOH
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Microchambers with E. coli Directionality
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Microvortex Created by E. coli
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Axial Fluid Flow in an Arched Microchannel Produced by Flagellar Motion of E. coli
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Orbital Revolution of a PMMA microdisk by E. coli
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Different Strategies in Photofabrication
With Photomask and Confocal Scanner
With Digital Micromirror Device
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Fabrication Process with DMD
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Some Structures Fabricated with DMD
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References
Kaehr, B. and J. B. Shear (2008). "Multiphoton fabrication of chemically responsive protein hydrogels for microactuation." PNAS 105(26): 8850-8854.
Kaehr, B. and J. B. Shear (2009). "High-throughput design of microfluidics based on directed bacterial motility." Lab on a Chip 9(18): 2632-2637.
Nielson, R., B. Koehr, et al. (2009). "Microreplication and Design of Biological Architectures Using Dynamic-Mask Multiphoton Lithography." Small 5(1): 120-125.