Pheromone in Drosophila
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Transcript of Pheromone in Drosophila
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Pheromone in Drosophila
Manhasset Science Research
Rachel Lii
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Need
http://www.cdc.gov/Features/dsMalariaSurveillance/dsMalariaSurveillance_450px.gif
Figure 1:The total cases of Malaria by State in the U.S in 2007.
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Knowledge Base
Figure 2: X-ray Crystal Structure of LUSH-cVA complex. The VA is completely enveloped within the protein.
http://www.uccc.info/for-healthcare-professional/cancer-center/cores/structural-biology/structural-biology.aspx
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A) Orienting
Literature ReviewB) Tapping
C) Singing/ waving and wing vibrationD) Licking
E) Attempting
F) Copulation
http://www.springerlink.com/content/eq0711q8445h3w40/fulltext.pdf
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Literature Review
• (Smith, 2006) Odorant binding proteins are extracellular proteins localized to the chemosensory systems of most terrestrial (belonging to land) species.
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Literature Review
• (Smith et al, 2005) OBP lush is completely devoid (lacking of something) of evoked (cause reaction) activity to the pheromone 11-cis vaccenyl acetate (cVA), reveals a binding protein is absolutely required for activation of pheromone sensitive chemosensory neurons.
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Purpose
• Pest insects have a huge impact on agriculture and human health.
• Since Drosophila are similar to mosquitoes, using the pheromone baiting technique with LUSH pheromone.
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Hypothesis
ALTERNATE HYPOTHESIS: When the Mutant lush drosophila are in the presence of the pheromone they will not have a response, or they will not have a strong response to the pheromone. However, when the regular drosophila are in the presence of the pheromone, they will have a stronger reaction to it.
NULL HYPOTHESIS: When both the mutant and the regular drosophila are within the presence of the pheromone there will be no significant reaction.
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MethodologyDrosophila melanogaster
Mutant Drosophila
Data will be analyzed using SPSS and Scheffe-post-hoc test.
Regular Drosophila
Group 1N=20
Placed in oviposition trap
Group 2N=20
Group 3N=20
Group 6N=20
Group 4N=20
Group 5N=20
Group 7N=20
Group 8N=20
1 µl of Acetoxy-5-hexadecanolide inserted into the food
1.5 µl of Acetoxy-5-hexadecanolide inserted into the food
2.5 µl of Acetoxy-5-hexadecanolide inserted into the food
2 µl of Acetoxy-5-hexadecanolide inserted into the food
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Protocols For Measurement
• The trap BR-OVT (is composed of a simple black plastic box (13 x 35 x 24 cm) with a central window (16 x 9 cm) on the top surface
• The least harmful to the flies is either carbon dioxide or cooling anesthetizing
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Do-Ability
• BR-OVT are easy to construct
• Drosophila are easy to keep alive
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Budget
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Bibliography
• Department of Pharmacology and Center for Basic Neuroscience, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9111, USA
• Ha, Tal Soo, and Dean P. Smith. "A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate Induced Responces in Drosophila." The Journal Of Neuroscience (2006): 8727-733.
• Jin, Xin, Tal Soo Ha, and Dean P. Smith. "PNAS." SNMP is a signaling component required for Pheromone Sensitivity in Drosophila 31st ser. 105 (Ausgust 5, 2008): 10996-1001.
• "Pheromone." McGraw-Hill Encyclopedia of Science & Technology. 8th ed. 13 vols. Mechanicsburg: McGraw-Hill, 1997. 397-98.
• Smith, Dean P. "Odor and Pheromone detection in Drosophila melanogaster." Pflugers Arch-Eur J Physiol (2007): 749-58.
• University Of Texas Southwestern Medical Center At Dallas. "Researchers Find How Protein Allows Insects To Detect And Respond To Pheromones." ScienceDaily 27 January 2005. 4 May 2009 <http://www.sciencedaily.com /releases/2005/01/050124005139.htm>.
• UT Southwestern Medical Center. "Unique Pheromone Detection System Uncovered." ScienceDaily 27 June 2008. 29 April 2009 <http://www.sciencedaily.com /releases/2008/06/080626125825.htm>.
• Xu P-X, Atkinson R, Jones DNM, Smith DP (2005) Drosophila OBP LUSH is required for activity of pheromone sensitive neurons. Neuron 45:193-200