Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr....
-
Upload
imogene-jackson -
Category
Documents
-
view
217 -
download
0
Transcript of Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr....
![Page 1: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/1.jpg)
Pharmacology – II [PHL 322]
The Basic Principles of Central Synaptic Neurotransmission
Dr. Mohammad Nazam Ansari
![Page 2: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/2.jpg)
• SYNAPTIC TRANSMISSION
• The definition of synaptic transmission is simply the communication between two nerve cells. Communication believed to involve specialized structures termed "synapses".
• Charles Sherrington (1897) : named ‘Synapse’
Introduction
![Page 3: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/3.jpg)
• CNS Synapses
• Axodendritic: Axon to dendrite
• Axosomatic: Axon to cell body
Types of Synapses
• Axoaxonic: Axon to axon
• Dendrodendritic: Dendrite to dendrite
![Page 4: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/4.jpg)
Principles of Synaptic Transmission
• Basic Steps
• Neurotransmitter synthesis
• Load neurotransmitter into synaptic vesicles
• Vesicles fuse to presynaptic terminal
• Neurotransmitter spills into synaptic cleft
• Binds to postsynaptic receptors
• Biochemical/Electrical response elicited in postsynaptic cell
• Removal of neurotransmitter from synaptic cleft
• Must happen RAPIDLY!
![Page 5: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/5.jpg)
![Page 6: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/6.jpg)
• Neurotransmitters: “Substance that is released at a synapse by one neuron and that affects a postsynaptic cell, in a specific manner”
• Amino acids• Amines• Peptides
Principles of Synaptic Transmission
![Page 7: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/7.jpg)
• Neurotransmitters
• Small molecules synthesized in the terminal button and packaged in synaptic vesicles. E.g. Amino acids and amines are stored in synaptic vesicles
• Large molecules assembled in the cell body, packaged in vesicles, and then transported to the axon terminal. E.g. Peptides are stored in and released from secretory granules- Often coexist in the same axon terminals
Principles of Synaptic Transmission
![Page 8: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/8.jpg)
• Neurotransmitter Synthesis and Storage
Principles of Synaptic Transmission
![Page 9: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/9.jpg)
![Page 10: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/10.jpg)
• Release of Neurotransmitter (NT) Molecules : • Exocytosis – the process of NT release • A nerve impulse reaches the terminal knob of a neuron, causing the pre-
synaptic membrane to depolarize.• The depolarization of the pre-synaptic membrane causes voltage gated-
calcium-channels to open.• The entry of Ca2+ causes vesicles to fuse with the terminal membrane
and release their contents
Principles of Synaptic Transmission
![Page 11: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/11.jpg)
• Neurotransmitter Release
• Secretory granules- Released from membranes that are away from the active
zones
- Requires high-frequency trains of action potentials to be released
Principles of Synaptic Transmission
![Page 12: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/12.jpg)
• Neurotransmitter receptors: There are multiple receptor types for a given NT
• Ionotropic: Transmitter-gated ion channels- Ligand-binding causes a slight conformational change that leads to the
opening of channels- Depending on the ions that can pass through, channels are excitatory
or inhibitory- NT binds and an associated ion channel opens or closes, causing a
PSP. If Na+ channels are opened, an EPSP occurs. If K+ channels are opened, an IPSP occurs
Excitatory and Inhibitory Postsynaptic Potentials:• EPSP: Transient postsynaptic membrane depolarization by presynaptic
release of neurotransmitter. E.g. Ach- and glutamate-gated channels cause EPSPs
• IPSP: Transient hyperpolarization of postsynaptic membrane potential caused by presynaptic release of neurotransmitter. E.g. Glycine- and GABA-gated channels cause IPSPs
Principles of Synaptic Transmission
![Page 13: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/13.jpg)
OUT
IN
Cl-
Cl-
Na+
Na+
GABAA receptor Glutamate/AMPAreceptor
GA
BA
Gl
u
Inhibition Excitation
![Page 14: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/14.jpg)
• Metabotropic: G-protein-coupled receptors- Trigger slower, longer-lasting and more diverse postsynaptic actions- Same neurotransmitter could exert different actions depending on what receptors
it bind to- (1) NT 1st messenger binds. (2) G protein subunit breaks away. (3) Ion channel
opened/closed OR a 2nd messenger is synthesized. (3) 2nd messengers may have a wide variety of effects.
• Autoreceptors: present on the presynaptic terminal - Typically, G-protein coupled receptors- Commonly, inhibit the release or synthesis of neurotransmitter- Negative feedback
Effector proteins
Principles of Synaptic Transmission
![Page 15: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/15.jpg)
![Page 16: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/16.jpg)
• Neurotransmitter Reuptake, Enzymatic Degradation, and Recycling
• As long as NT is in the synapse, it is active – activity must somehow be turned off
• Clearing of neurotransmitter is necessary for the next round of synaptic transmission
- Simple DiffusionReuptake aids the diffusionNeurotransmitter re-enters presynaptic axon terminal or enters
glial cells through transporter proteinsThe transporters are to be distinguished from the vesicular forms
- Enzymatic destructionIn the synaptic cleftAcetylcholinesterase (AchE)
Principles of Synaptic Transmission
![Page 17: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/17.jpg)
![Page 18: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/18.jpg)
• Neuropharmacology
• The study of effect of drugs on nervous system tissue
• Receptor agonists: Mimic actions of naturally occurring neurotransmitters- E.g. Nicotine binds and activates the Ach receptors of skeletal muscle
(nicotinic Ach receptors)
• Receptor antagonists: Inhibitors of neurotransmitter receptors- e.g. Curare binds tightly to Ach receptors of skeletal muscle
• Toxins and venoms
• Defective neurotransmission: Root cause of neurological and psychiatric disorders
Principles of Synaptic Transmission
![Page 19: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/19.jpg)
DOPAMINE• D1, D2, D3, D4, D5 receptors; all metabotropic• D1, D5: all postsynaptic, and increase adenylate cyclase (AC)• D2, D3, D4: presynaptic and postsynaptic, and decrease AC
Dopamine pathways do many things:• Control flow of blood through the brain• Motor control (nigrostriatal) system• Behavioural control: Dopamine is the brain’s motivational
chemical. The primary role of dopamine is pleasure and motivation.
A shortage of brain dopamine causes an indecisivepersonality, unable to initiate even the body’s own movement. Parkinson’s disease.
Excess dopamine, more arousal. Attention deficit disorder. May cause schizophrenia.
![Page 20: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/20.jpg)
Neurotransmitters and Neuromodulators
• catecholamines synthesized from tyrosine
• indoleamines synthesized from tryptophan
Catecholamine biosynthesis indoleamine biosynthesis
![Page 21: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/21.jpg)
SEROTONIN
- at least 14 different receptor subtypes- 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E, 5-HT1F; all metabotropic- 5-HT2A, 5-HT2B, 5-HT2C; all metabotropic- 5-HT3; ionotropic, Cl- channel, inhibitory input- 5-HT1B and 5-HT1D are presynaptic autoreceptors
![Page 22: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/22.jpg)
A synapse that uses serotonin/5-HT
![Page 23: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/23.jpg)
Re-uptake of 5-HT/serotonin
Fluoxetine/Prozac blocks the SERT
Treatment of depression.anxiety disorders, obsessive-compulsive disorders
![Page 24: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/24.jpg)
5–hydroxytryptamine (Serotonin)Functions : 1. Addiction, aggression, anxiety, impulsivity 2. Learning, memory, mood 3. Emesis, nausea, appetite 4. Penile erection, sexual behavior 5. Sleep, 6. Thermoregulation 7. Respiration 8. Vasoconstriction 9. Locomotion
Deficiencies in the Function of SerotoninAnxiety, depression, obsessive-compulsive disorder, schizophrenia, stroke, obesity, pain, hypertension, vascular disorders, migraine, and nausea to disruptions and particularly deficiencies of serotonin.
![Page 25: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/25.jpg)
5–hydroxytryptamine (Serotonin)Clinical uses : 1. Antidepressants & anxiolytics 2. Atypical antipsychotics:3. Anorectics (decreases appetite): releases 5HT4. Antiemetics :5. Gastroprokinetic agents:6. Antimigraine agents 7. Increases appetite: 5-HT2A blocker
![Page 26: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/26.jpg)
Glutamate• Excitatory neurotransmitter
• Located – throughout CNS
• Receptor types
a) Ionotropic receptors
i) NMDA – long duration of action (Ca+ Channel)
ii) AMPA – fast action (Na+ Channel)
iii) Kainic acid – fast action (Na+ Channel)
b) Metabotropic (GPCR) receptors: autoreceptor
![Page 27: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/27.jpg)
Glutamate – clinical use
1. Alzheimers disease, influenza
2. Cough suppressant
3. Anesthesia
4. Stroke
5. Epilepsy
6. Diabetic neuropathic pain
7. Senile dementia
8. Suppress withdrawal symptoms from morphine
![Page 28: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/28.jpg)
Gama Amino Butyric Acid (GABA) :
• synthesized from glutamic acid by GAD• Actions - Major inhibitory neurotransmitter (NT) • Location – Widely distributed in brain & spinal cord
• Receptor types & actions – a) GABAA - Ionotropic, Cl- influx, postsynaptic receptor
- fast IPSP b) GABAB - Metabotropic, GPCR,
- K+ activate channel , reduce Ca2+ conductance, inhibit adenyl cyclase
- slow & long lasting IPSP c) GABAC - Cl- influx
![Page 29: Pharmacology – II [PHL 322] The Basic Principles of Central Synaptic Neurotransmission Dr. Mohammad Nazam Ansari.](https://reader035.fdocuments.us/reader035/viewer/2022062715/56649d9e5503460f94a88589/html5/thumbnails/29.jpg)
Clinical uses – GABA related drugs : 1. As antiepileptics 2. As anesthetics 3. Sedative hypnotics ( BZD, barbiturates)
- anxiety - insomnia - sedation & amnesia - component of anesthesia - control of ethanol or sedative-hypnotic withdrawal
state - muscle relaxants
4. Migraine headache prophylaxis – - valproate, topiramate 5. Spasmolytics :stroke, cerebral palsy, multiple sclerosis
- baclofen, diazepam