January 17, 2014

PerkinElmer Introduces a Pre-Clinical High-Throughput Bioluminescence Small Animal Imaging System SUMMARY: PerkinElmer introduces the IVIS® SpectrumBL, developed to enable the

pharmaceutical and biotech industries to improve efficiencies in pre-clinical imaging studies by increasing in vivo throughput for drug efficacy, safety and toxicology.

The IVIS SpectrumBL high-throughput 2D and 3D optical imaging system is currently the only system that can simultaneously image up to 10 mice with full gas anesthesia incorporation along with ultra-high sensitivity to support bioluminescence, chemiluminescence and Cerenkov radioisotopic imaging. PerkinElmer’s leading optical imaging technology facilitates non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals to enable customers to quantitatively evaluate drug efficacy. The IVIS SpectrumBL features a 5 and a 10 mouse anesthesia manifold for larger cohorts, offering robust in vivo data, as well as significantly increasing the potential number of compounds that can be screened in vivo in early stages of pre-clinical drug discovery.

Media Contact: Anna Christensen Director of Marketing- In Vivo Imaging, PerkinElmer Email: anna.christensen@perkinelmer.com

# # # About PerkinElmer, Inc.

PerkinElmer, Inc. is a global leader focused on improving the health and safety of people and the environment. The company reported revenue of approximately $2.1 billion in 2012, has about 7,600 employees serving customers in more than 150 countries, and is a component of the S&P 500 Index. Additional information is available through 1-877-PKI-NYSE, or at www.perkinelmer.com.

PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA

Phone 781.663.6900 Fax 781.663.6052 www.perkinelmer.com

P R O D U C T N O T E

Pre-clinical in vivo imaging

The IVIS® SpectrumBL is an advanced high-throughput 2D and 3D optical imaging system designed to improve quantitative outcomes of bioluminescence, chemiluminescence and Cerenkov in vivo imaging.

The SpectrumBL supports 10 mice simultaneous imaging for true high-throughput imaging for longitudinal studies to support large cohorts of mice. It uses a patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals.

High-Throughput In Vivo Bioluminescence Imaging System

Key Features

• Ultrahigh-sensitivitytosupportin vivobioluminescence,chemiluminescenceandCerenkovimaging

• High-throughput(10mice)imagingenablement

• Highresolution(to20microns)with3.9cmfieldofview

• 3Ddiffusetomographicreconstructionforbioluminescence

• Co-register3DopticaldatawithmicroCT,PET/SPECTandMRI

• NISTtraceableabsolutecalibration

• OptionalupgradepathtoanIVISSpectrumforfullfluorescenceenablement

IVIS SpectrumBL

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UTI Pneumonia Meningitis

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High-Throughput Bioluminescence Imaging

The IVIS SpectrumBL provides the best in class bioluminescence sensitivity, a standard with all IVIS imaging systems, with the ability to image 10 mice (Figure 1) at once. The 10 mouse manifold that comes standard with SpectrumBL improves drug discovery workflow by reducing imaging times for longitudinal studies in half. The chart (Figure 1) shows that with SpectrumBL

Bioluminescence Imaging - Best in Class In Vivo Sensitivity

IVIS SpectrumBL has a cooled (-90 °C) camera with large CCD chip area and low F-stop for high sensitivity bioluminescent light detection. Image multiple bioluminescent reporters such as firefly luciferase, Renilla luciferase and bacterial luciferase

120% more compounds can be evaluated annually in vivo. Getting more compounds screened for target or biomarker validation in vivo during the earlier stages of pre-clinical drug discovery significantly improves efficiency in later clinical stage development.

in vivo at depth with rapidly and quantitatively. The ultra sensitive camera optics allows single cell detection, earlier monitoring of micrometastasis in vivo and track tumor development longitudinally in vivo. Other applications include infectious disease (Figure 3), stem cell tracking and toxicology.

Figure 1. A representative image of ten mice being imaged in vivo. Chart on the right illustrates how significantly more drug candidates can be evaluated in vivo due to the high-throughput imaging capabilities of SpectrumBL.

Figure 2. Single cell detection of 4T1-luc2 cells injected subcutaneously in nude mice (A), monitoring NCI-H460-luc2 lung tumor growth by NCI-H460-luc2 (B) and metastasis post intracardiac injection of MDA-MA-231-luc2 cells longitudinally (C).

Figure 3. Tracking infection progression in models of UTI, pneumonia and meningitis.

Click # OC20050216112737Wed, Feb 16, 2005 11:28:18Em filter=620Bin:M (8), FOV4.1, f2, 30sCamera: IVIS 200 Beta II, SI620EEV

Expt ID: GFAP_Xen10Pathogen: S. pneumo Xen 10+luciferinRoute of Infection: ICAnimal Number: m5 - closeup view of brainTime Point: 19h post infection

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Color BarMin = 60000Max = 5e+06

Click # OC20050216112500Wed, Feb 16, 2005 11:25:36Em filter=500Bin:M (8), FOV4.1, f1, 30sCamera: IVIS 200 Beta II, SI620EEV

Expt ID: GFAP_Xen10Pathogen: S. pneumo Xen 10+luciferinRoute of Infection: ICAnimal Number: m5 - closeup view of brainTime Point: 19h post infection

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Cerenkov Imaging – Optimized Software Tools for Faster Workflow

Living Image® software brings IVIS technology to life by facilitating an intuitive workflow for image acquisition, analysis and data organization Living Image software in SpectrumBL has new features such as an imaging mode optimized for Cerenkov imaging. The software guides the user for optimal camera parameter settings for high signal-to-noise when detecting light emitted from radionuclide-injected animal subjects.

The optional DyCE™ mode makes it very easy to set up biodistribution scans of radiopharmaceuticals. Spectrally unmix radionuclides from other light-emitting probes of very different spectra. The DyCE technique acquires a series of dynamic images following an injection of radionuclide. The location of major internal organs is derived by proprietary algorithms and displayed in minutes (Figure 4). The DyCE software module includes the Multi-View platform and software that extends the functionality of Living Image and is available for all IVIS systems.

Advanced 3D Analysis with MicroCT Co-registration Tools

Look deeper, see further, and take science to a new level of sophistication with the 3D technology from PerkinElmer. 3D diffuse luminescence tomography (DLIT) utilizes structured light data with bioluminescence images to reconstruct three dimensional representations of bioluminescent reporters. Take the next step and analyze 3D sources in an anatomical context with the Digital Mouse Atlas (Figure 5).

The tomography tool allows the quantification of the number of cells in a tumor and 3D co-registration of bioluminescent reporter. Seamlessly co-register 3D bioluminescence with Quantum FX microCT (Figure 6).

Figure 4. A mouse bearing a subcutaneous 4T1-luc2 tumor in its right flank was injected with 315 μCi of 18F-FDG intravenously. The animal was imaged dynamically starting 55 seconds post-injection to capture the distribution of 18F-FDG in the mouse body via Cerenkov light from positron emission.

Figure 5. DLIT 3D reconstruction shows precise localization of GL261-luc2 brain tumor using digital mouse atlas.

Figure 6. Optical-CT co-registration of osteolytic tumors with Quantum FX microCT. Mice were implanted with MDA-MB-231-luc-D3H2Ln cells by intracardiac injection.

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Inside the IVIS SpectrumBL

Imaging Chamber

• Light-tight imaging chamber

• Heavy-duty castors

• Integrated gas anesthesia

• LED lamps for photographic images

• Heated stage to maintain optimum body temperature

• Electromagnetic door latch

• Motor-controlled stage, filter wheel, lens position, and f-stop

• Scanning laser for mouse alignment and surface topography

CCD Camera

• Back-thinned, back-illuminated grade 1 CCD provides high quantum efficiency over the entire visible to near-infrared spectrum

• 13.5 micron pixels, 2048 x 2048

• 16-bit digitizer delivers broad dynamic range

• CCD is thermoelectrically (Peltier) cooled to -90 °C, ensuring low dark current and low noise

Custom-Designed Lens

• 6-inch diameter optics, f/1– f/8

• High-resolution – down to 20 microns

The Spectrum Series platform can be tailored to your workflow and is available in three models: IVIS SpectrumBL, IVIS Spectrum and IVIS SpectrumCT

Features IVIS SpectrumBL IVIS Spectrum IVIS SpectrumCT

Animal Capacity 10 mice 5 mice 5 mice

Bioluminescence 3 3 3

Fluorescence 3 3

Full Spectral Tunability 3 3

Epi-Illumination 3 3

Trans-Illumination 3 3

3D Fluorescence Tomography 3 3

3D Bioluminescence Tomography 3 3 3

Quantification 3 3 3

Absolute Calibration 3 3 3

3D Multimodal Co-Registration (PET, CT, MRI) 3* 3 3

Integrated X-Ray and microCT 3

Compute Pure Spectrum - Spectral Unmixing 3 3

Optimized NIR Excitation Lightsource N/A Extended NIR Range 150W Tungsten EKE

Detector type 1" Back-thinned, back-illuminated Grade 1 CCD

Camera Temp -90°C

Imaging Pixels 2048 x 2048

Accessory Line Isolation chamber, Anesthesia, calibration tools, phantom mice, Multimodality Software and Mouse Imaging shuttle, DyCE Imaging, Multi View Imaging

Custom Lens, and Emission Filter Wheel

Gas Anesthesia Manifold

CCD Camera

Scanning laser assembly

Imaging Chamber Heated Shelf

Soundproof Portable Cart, with a 25x34-inchFootprint

* For bioluminescent reporters only

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IVIS SpectrumBL- Complete Imaging Solutions

XGI - 8 Anesthesia System Cat No. 118918

Animal Isolation Chamber Kit XIC - 3 Cat No. 123997

Mouse Imaging Shuttle Cat No. 127744

Animal Shield Kit XAS-3 Cat No. 119002

XWS - 260 Workbench Cat No. 119207

IVIS Syringe Injection System Cat No. 124633

Luciferin Luciferase Cell Lines Lentiviral Particles

For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs

Copyright ©2013, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. 011478_01

PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602www.perkinelmer.com

For more information, please visit our website at www.perkinelmer.com/invivo

Imaging System Components Specifications

Heated Chamber Yes

Gas Anesthesia Ports Yes

Injector Ports Yes

Imaging Chamber Interior Size 43 x 50 x 60 cm (W x D x H)

Imaging System Space Requirement 203 x 163 x 214 cm (W x D x H)

Power Requirements 20 Amps for 120 VAC or 10 Amps for 230 VAC

Stage Temperature 2 0-40 °C

Computer Quad Core 2.8 GHz, 12 GB, 1333 MHz DDR3, SDRAM, 2GB NVIDIA Quadro 4000 with 256 CUDA Cores, I TB hard drive, 20" flat screen monitor

Optical Specifications

Camera Sensor Back-thinned, back-illuminated Grade 1 CCD

CCD Size 2.7 x 2.7 cm

Imaging Pixels 2048 x 2048

Quantum Efficiency > 85% 500-700 nm; > 30% 400-900 nm

Pixel Size 13.5 microns

Min. Detectable Radiance 70 photons/s/sr/cm2

Min. Field of View (FOV) 3.9 x 3.9 cm

Max. Field of View (FOV) 23 x 23 cm

Min. Image Pixel Resolution 20 microns

Lens f/1 – f/8 1.5x, 2.5x, 5x, 8.7x magnifications

Read Noise < 3 electrons for bin=1,2,4; < 5 electrons for bin=8,16

Dark Current (Typical) < 100 electrons/s/cm2

3D Tomography Software Included (Bioluminescent reporters only)

CCD Operating Temperature -90 °C