Peripheral Nervous System Products Human iPSC-Derived ...
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PHONE: +44 (0) 12237 751051 U.S. TOLL FREE: 1-800-678-2965 EMAIL: [email protected] https://www.axolbio.com/ Peripheral Nervous System Products Human iPSC-Derived Sensory Neurons DAY 1 DAY 10 DAY 14 Our Maturation Maximizer media supplement ensures our iPSC-derived sensory neurons mature faster than those cultured in our traditional maintenance media. It works by mimicking in vivo signals between sensory neurons and their supporting cells. The supplement contains signaling factors present in the peripheral nervous system and in particular the native environment of sensory neurons. Utilizing this supplement accelerates the maturation of iPSC-derived sensory neurons in vitro. Accelerated Maturation with our Maximizer Supplement Human iPSC-Derived Sensory Neurons Sensory Neuron Culture Media and Reagents CATALOG NUMBER & PRODUCT NAME DONOR STARTING MATERIAL QUANTITY ax0055 - Human iPSC-Derived Sensory Neuron Progenitors Male, Newborn Cord blood CD34+ cells 500,000 cells per vial CATALOG NUMBER & PRODUCT NAME QUANTITY ax0058 - Sensory Neuron Maturation Maximizer Supplement 1 x 1mL vial ax0060 - Sensory Neuron Maintenance Medium 250 mL Axol iPSC-derived sensory neurons express sodium ion channels Nav1.7 and the DRG-specific, TTX-resistant channels, Nav1.8 and Nav1.9 as well as the temperature-sensitive, TRPV1 and TRPM8, and TRPA1, a sensor of pungency, bitterness and cold. Axol iPSC-Derived Sensory Neuron Progenitors are available in large batch sizes for reliable and consistent results in high-throughput screening assays. The cells are also suitable for investigating disorders of the peripheral nervous system and chronic pain.
Transcript of Peripheral Nervous System Products Human iPSC-Derived ...
PHONE: +44 (0) 12237 751051 U.S. TOLL FREE: 1-800-678-2965 EMAIL: [email protected] https://www.axolbio.com/
Peripheral Nervous System Products
Human iPSC-Derived Sensory Neurons
DAY 1 DAY 10 DAY 14
Our Maturation Maximizer media supplement ensures our iPSC-derived sensory neurons mature faster than those cultured in our traditional maintenance media.
It works by mimicking in vivo signals between sensory neurons and their supporting cells. The supplement contains signaling factors present in the peripheral nervous system and in particular the native environment of sensory neurons. Utilizing this supplement accelerates the maturation of iPSC-derived sensory neurons in vitro.
Accelerated Maturation with our Maximizer Supplement
Human iPSC-Derived Sensory Neurons
Sensory Neuron Culture Media and Reagents
CATALOG NUMBER & PRODUCT NAME DONOR STARTING MATERIAL QUANTITY
ax0055 - Human iPSC-Derived Sensory Neuron Progenitors Male, Newborn Cord blood CD34+ cells 500,000 cells per vial
CATALOG NUMBER & PRODUCT NAME QUANTITY
ax0058 - Sensory Neuron Maturation Maximizer Supplement 1 x 1mL vialax0060 - Sensory Neuron Maintenance Medium 250 mL
Axol iPSC-derived sensory neurons express sodium ion channels Nav1.7 and the DRG-specific, TTX-resistant channels, Nav1.8 and Nav1.9 as well as the temperature-sensitive, TRPV1 and TRPM8, and TRPA1, a sensor of pungency, bitterness and cold.
Axol iPSC-Derived Sensory Neuron Progenitors are available in large batch sizes for reliable and consistent results in high-throughput screening assays. The cells are also suitable for investigating disorders of the peripheral nervous system and chronic pain.
Peripheral Nervous System Products
Axol is a leading provider of product and service solutions in the iPSC-based neuroscience, immune cell, and cardiac modeling for drug discovery and screening markets. Our custom research capabilities in gene editing, electrophysiology, reprogramming and
differentiation means we can offer customers validated ready-to-use cell lines and a suite of services bolstered by deep scientific expertise and robust functional data - all with shorter lead times.
Discovery Stems From HereAxol Bioscience LtdScience Village, Chesterford Research ParkLittle Chesterford, CambridgeUnited Kingdom, CB10 1XL
PHONE: +44 (0) 1223 751051US TOLL FREE: 1-800-678-AXOL (2965)EMAIL: [email protected]
Copyright © 2021 All Rights Reserved by Axol Bioscience Ltd.
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Figure 2. Immunocytochemistry staining for Sensory Neuronal markers.
Culturing our sensory neuron progenitor cells with our Maximizer supplement speeds up maturation. On average we see increases of 30-45% of neurons expressing TRPv1, Nav1.7 and Nav1.8 in week 4 of culture (Figure 2).
Significantly Increased Key Marker Expression
Sensory Neurons are Functionally Mature
TUJ-1, DAPI NaV1.7, DAPI NaV1.8, DAPI TrpV1, DAPI
Using the Maximizer supplement accelerates functional maturity, and ~90% of neurons are sensitive to capsaicin by day 22 of culture. They also respond to other pharmacological and physical stimuli such as mustard oil, menthol and temperature change (Figure 3).
Figure 3. MEA recordings show neuronal responses to sensory stimuli.
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In addition to our cryopreserved stock we offer a custom differentiation process to generate human iPSC-derived sensory neurons that fulfill your R&D requirements (Figure 1).
Custom Sensory Neuron Differentiation Figure 1. Schematic of the differentiation process to produce Sensory Neurons.
