PCRBrBtB B AíB BtBzB·BpB BjB¤B· ep realplex · A marvel in space and time B¥B]B¦BzB_B o2...

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A marvel in space and time PCR ® ep realplex

Transcript of PCRBrBtB B AíB BtBzB·BpB BjB¤B· ep realplex · A marvel in space and time B¥B]B¦BzB_B o2...

A marvel in space and timePCR ® ep realplex

ep

PCR

PCR

ep realplex

Maximun speed, minimum space

ep realplex

Two times twice four

PCR 2

ep realplex2 ep realplex4

PCR LED

(PMT)

PCR

Upgrade- any time

realplex ep

PCR real-

plex2 4

Eppendorf engineering

ep realplex

PCR

Eppendorf TSP(Thermal Sample Protection)

2

Small, fast, flexible!

Mastercycler® ep realplex—licensed real-time PCR solutions

Mastercycler ep realplex:real-time PCR without limits

Quality in all form

ep realplex ep

epPCR ep realplexrealplex 96

epSteadyslope

296 ’’high-

speed’’ impulse PCR 96

PCR

2 realplex2

reaplex4 realplex2 21 (PMT) real-

plex4 4 42 PMT

3

Unrestricted!

realplex

PCRrealplex

4

Mastercycler ep realplex: optical highlight.

96

96 LEDLED

LED 470nmPCR

( SYBR Green, FAM, VIC, TET, HEX, ROX, JOE, TAMRA)

96

10-30nmPMT

realplex PMTCPM(channel-photo-multiplier)

Mastercycler ep realplex light path96 LED (470nm)

a/b/c 96

( )

“96-in-1”

5

realplex109 <50fM

ep realplex

PCRSNPs

6

Programmed for success!

Results in real-time

6 realplex

Microsoft Excel

realplex ’’ ’’

PCR PCRPCR

7

ep realplex

DNA CtCt

DNA

ep realplex ’’ ’’

Ct( )

Description

Mastercycler® ep realplex:

® ep realplex2, with aluminum block and two emission filters 6300 000.507 96751 5,100,000® ep realplex2 S, with silver block and two emission filters 6300 000.604 96752 5,400,000® ep realplex4, with aluminum block and four emission filters 6302 000.504 96753 6,200,000® ep realplex4 S, with silver block and four emission filters 6302 000.601 96754 6,400,000

Consumables

PCR 96 - (25 / ) 0030 128.575 95576 14,500

5390 000.016 95546 210,000 (100 / ) 0030 127.650 95545 18,000

Technical specification: Mastercycler ep realplex

96 LEDs (470 nm)

520 / 550 nm (realplex2)520 / 550 / 580 / 605 nm (realplex4)

1- photomultiplier (realplex2)2- photomultiplier (realplex4)

9

50 fM

96 x 0.2 ml PCR 1 x 96 PCRSBS

4 -99

1 -20

1 -24

30 -99

105

35 0.390 0.4

0.2

4 /s ( ep) 6 /s ( ep S)

3 /s ( ep) 4.5 /s ( ep S)

26 cm x 41 cm x 39.6 cm

24 kg

17 kg

7 kg

100V, 50 Hz-60 Hz

800W

*

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