Lori Mestre University of Illinois at Urbana-Champaign University of Illinois at Urbana-Champaign.
Paul Selvin class - University of Illinois Urbana-Champaign
Transcript of Paul Selvin class - University of Illinois Urbana-Champaign
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KlausSchultenTheoretical and Computational Biophysics Group
February 15, 2012
A glimpse of computational methods in biological physics:
Case study on three proteins and a cellular organelle
PHYS 498: Introduction to Biological PhysicsLoomis 158
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Administrative issues
Assignment from this lecture: read the case study on ubiquitin and choose FOUR out of the six exercises to complete: http://www.ks.uiuc.edu/Training/CaseStudies/index.html#ubqcs
You will have to download and install the software VMD: http://www.ks.uiuc.edu/Research/vmd/
For any questions and assistance on the assignment, email Ivan Teo at [email protected]
Assignments should be given to Professor Selvin before class on February 22, 2012 (next Wednesday).
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Introduction to Protein Structures -Molecular Graphics Tool
Ubiquitinamino acid tyrosine, Y
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Quick Overview of Protein Structure
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Peptide bond linking two amino acids
Proteins: polymeric molecules linking amino acids through peptide bonds
What Proteins are Made of: Primary Structure (Sequence) of Amino Acids
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The twenty amino acids
non-polar
polar
charged
URL: http://lectures.molgen.mpg.de/ProteinStructure
Protein Structure and Function, Gregory Petsko and Dagmar Ringe, 2004Molecular Biology of The Cell Alberts, Johnson, Lewis, Raff, Roberts, Walter, 2008, 3rd Ed.Introduction to Protein Structure, 2nd ed. Carl Branden & John Tooze, 1999
Protein Primary Structure
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Alanine
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Valine
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Phenylanaline
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Proline
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Methionine
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Isoleucine
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Leucine
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Aspartate
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Glutamate
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Lysine
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Arginine
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Serine
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Threonine
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Tyrosine
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Histidine
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Cysteine
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Asparagine
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Glutamine
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Tryptophane
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Glycine
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Looking at Proteins Through the Program VMD
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Learn to use VMD from the “Using VMD” tutorial available athttp://www.ks.uiuc.edu/Training/Tutorials/
VMD for Mac OS X, Unix, and Windows is available for download at http://www.ks.uiuc.edu/Research/vmd/
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Protein Secondary Structure
An alpha helix
An antiparallel beta sheet
The backbone is formed as a helix. An ideal alpha helix consists of 3.6 residues per complete turn. The side chains stick out. There are hydrogen bonds between the carboxy group of amino acid and the amino group of another amino acid n+4. The mean phi angle is -62 degrees and the mean psi angle is -41 degrees
Beta sheets are created, when atoms of beta strands are hydrogen bound. Beta-sheets may consist of parallel strands, antiparallel strands or out of a mixture of parallel and antiparallel strands.
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Tertiary structure describes the packing of alpha-helices, beta-sheets and random coils with respect to each other on the level of one whole polypeptide chain.
Quaternary structure only exists, if there is more than one polypeptide chain present in a complex protein. Then quaternary structure describes the spatial organization of the chains
Tertiary and Quarternary Structures of Proteins
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• 76 amino acids
• highly conserved
• covalently attaches to proteins and tags them for degradation
• other cell traficking
Focus on one proteinUbiquitin
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Mono-ubiquitylation versus multi-ubiquitylation
Marx, J., Ubiquitin lives up its name, Science 297, 1792-1794 (2002)
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M. Hochstrasser, Nature, 458. (2009)
proteasome
The substrate-polyubiquitin complex is then sent to proteasome
The substrate is sent through the proteasome barrel, where it is chopped
up and recycled
Recognizes and catches polyubiquitin!
Ubiquitin’s role in protein degradation
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Polyubiquitine Ruler of teh Proteasome
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M. Hochstrasser, Nature, 458. (2009)
proteasome
The substrate-polyubiquitin complex is then sent to proteasome
The substrate is sent through the proteasome barrel, where it is chopped
up and recycled
Recognizes and catches polyubiquitin!
Ubiquitin’s role in protein degradation
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The sequence of ubiquitin is highly conserved, in particular the seven lysine residues
A lysine residue in a ubiquitin can be linked to the C-terminus of another ubiquitin
By using different lysine for such linkage, ubiquitin is used for different cellular purposes
Highly conserved ubiquitin chain
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VMD Demo
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http://en.wikipedia.org/wiki/Ubiquitin
J. Marx, “Ubiquitin lives up to its name.” Science, 297. (2002)
M. Hochstrasser, “Origin and function of ubiquitin-like proteins.” Nature, 458. (2009)
A. Varshavsky, “The early history of the ubiquitin field.” Protein Science, 15. (2006)
C. M. Pickart, “Back to the future with ubiquitin.” Cell, 116. (2004)
M. Carrion-Vazquez et al., “The mechanical stability of ubiquitin is linkage dependent.” Nature Structure Biology, 10. (2003)
http://nobelprize.org/nobel_prizes/chemistry/laureates/2004/
If you need to get a quick lesson on VMD, here is a short tutorial:
http://www.ks.uiuc.edu/~jhsin/papers/HSIN2008.pdf
Some readings
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villin headpiece
Observe folding process in unprecedented detail
Protein Folding
• Folding of the Protein called Villin Headpiece
• First protein folded in computer simulation
• Visualization of the “trajectory” of the folding protein reveales how this protein finds it native conformation from an initially stretched-out conformation
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VMD Demo
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hν
ADP ATP
purple bacterium cell
photosynthetic unit
energy converter
Schematic arrangement of
all six protein types
The proteins that make up the chromatophore of photosynthetic bacteria
20020
1
10
5
stoichiometry 200:20:20:10:5:1
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hν membrane
LH-IILH-I
cytochrome c2
e-
H+
H+
ADP ATPcytoplasm
periplasm
RC bc1
Q/QH2/Q ATPase
Chromatophore of Purple Bacteria(section of the chromatophore membrane)
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Knowing the Atomic Level Structureof the chromatophore, one can systematically describe
its physical mechanism
ADP ATP
light
M. Sener, J. Olsen, N. Hunter, and K. Schulten. PNAS, 104: 15723-15728, 2007
Melih Sener
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VMD Demo
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BTRC for Biomolecular Modeling and Bioinformaticshttp://www.ks.uiuc.edu/
Beckman Institute, UIUC
QuickSurf Representation
w/ Angle-Modulated
Transparency
Chromatophore 10M atoms
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The “Physics” of Light Harvesting in the Chromatophore
links: induced dipole - induced dipole interaction
Calculated Energy Transfer Rates Determine Optimal Placement of Proteins in Chromatophore
M. Sener, J. Olsen, N. Hunter, and K. Schulten. PNAS, 104: 15723-15728, 2007