Pathway to Knowing “Everything” - NCSU Libraries · Techniques in Necropsy: Pathway to Knowing...
Transcript of Pathway to Knowing “Everything” - NCSU Libraries · Techniques in Necropsy: Pathway to Knowing...
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Techniques in Necropsy: Pathway to Knowing “Everything”
Christopher KoivistoNCSU-CVM Class of 2011
Summer Zoo RoundsJune 12, 2008
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The Post-Mortem Exam
Patient historyNecropsyHistopathologyMicrobial culture/isolationToxicity screeningGene analysis
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DefinitionNecropsy
“seeing a dead body” (Greek)
Spyglass Entertainment Group, The Sixth Sense , 1999
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Why do a necropsy?DiagnosticPreventionEvaluate health of a populationEducational
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Why do a necropsy?
http://www.isrvma.org/article/54_3_7.htm
http://w3.vet.cornell.edu/nst/nst.asp?Fun=Image&imgID=1575
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Necropsy Facility
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Necropsy Facility
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Necropsy Facility
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Basic EquipmentSharp knife and steelScissorsTree limb snipsHandsawRongeursRulerTissue containers
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Basic Equipment
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Basic Equipment
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Basic Equipment
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Basic Equipment
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Goals of Necropsy
Data collectionConcise lesion descriptionAppropriate tissue samplingCorrelate findings with in-life data.
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Data CollectionReview historySystematicNecropsy record
Signalment and IDDate/time of deathDate of necropsy List of tissues examinedDescription of lesions
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Necropsy Record
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Necropsy Record
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Lesion DescriptionTissueLocationColorSizeShape
http://w3.vet.cornell.edu/nst/nst.asp?Fun=Image&imgID=6708
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Lesion DescriptionConsistency and textureNumber and extentSurface appearance Odor
http://w3.vet.cornell.edu/nst/nst.asp?Fun=Image&imgID=6708
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Tissue SamplingCytologyLight microscopyMicrobial isolationMolecular analysisToxicologyElectron microscopy
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CytologyPrior to fixationTouch impressionSmearsSkin scrapeFine needle aspirate (FNA)
http://w3.vet.cornell.edu/nst/nst.asp?Fun=Image&imgID=6292
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Light Microscopy
Formalin fixed<1cm thick>10:1 fixative:tissue ratioRepresentative samples should include junction between normal and abnormalHistochemistry and immunohistochemistry
http://w3.vet.cornell.edu/nst/nst.asp?Fun=Image&imgID=9531
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Microbial IsolationFresh tissue Transport mediaFrozen tissue (viruses)
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Molecular AnalysisPathogen identificationFrozen samples are preferred
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ToxicologyFresh or frozen tissueLiver, kidney, skeletal muscle, fat
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Electron Microscopy1% glutaraldehydeTissue perfusionParaffin embedded tissue
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http://www.vetmed.ucdavis.edu/whc/pdfs/necropsy.pdf
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http://w3.vet.cornell.edu/nst/nst.asp?Fun=Image&imgID=8235
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http://w3.vet.cornell.edu/nst/nst.asp?Fun=Image&imgID=18902
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http://courses.science.fau.edu/~jwyneken/sta/SeaTurtleAnatomy- Methods_of_Dissection.pdf
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SummaryStart big; work towards smallBe consistentReturn to the animal’s historyCollect extra tissue if unsureDo it as often as you can
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Want Necropsy Experience?
Gross Pathology RoundsEvery Wednesday 12:15-1:15
Saturday Morning Necropsy Volunteers
See PathHeads board for available dates
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ReferencesDepartment of Defense. Veterinary Necropsy Report Checklist andGuidelines (DD Form 1626). Oct. 2001. 10 June 2008
<http://www.dtic.mil/whs/directives/infomgt/forms/eforms/dd1626.pdf>.
Echols, Scott M. Exotic Pet Medicine and Surgery. V.2, Avian Necropsy and Cytology [1 CD-ROM] : Basic Avian Techniques. Jackson, Wyoming: Teton Newmedia, 2003.
King, John M. Dr. John M. King's Necropsy Show and Tell. 26 Dec. 2007. 10 June 2008 <http://w3.vet.cornell.edu/nst/nst.asp>.
King, John M., Lois Roth-Johnson, and David C. Dodd. The Necropsy Book. Gurnee, IL: Charles Louis Davis DVM Foundation, 2005.
Munson, Linda. Necropsy of Wild Animals. 10 June 2008<http://www.vetmed.ucdavis.edu/whc/pdfs/necropsy.pdf>.
National Cancer Institute. Registry of Tumors in Lower Animals. 30 Sep. 2007. 10 June 2008 <http://www.pathology-registry.org/index_1.asp>.
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ReferencesRose, Karrie, Scott Newman, and Marcela Uhart. Wild Bird Highly Pathogenic Avian Influenza Surveillance: Sample Collection from Healthy, Sick and Dead Birds. Rome, Italy: Food and Agriculture Organization of the United Nations, 2006.
Terrell, Scott P., and Brian A. Stacy. "Reptile Necropsy Techniques."Infectious Diseases and Pathology of Reptiles. Ed. Elliott R.Jacobson.
Boca Raton, FL: Taylor and Francis, 2007. 219-256.
USDA-APHIS. Animal Health: Collecting Samples for Diagnostic Unknowns. 25 Feb. 2008. 10 June 2008
<http://www.aphis.usda.gov/animal_health/lab_info_services/collection_submission.shtml>.
Woodford, M. H., D. F. Keet, and R. G. Bengis. Post-mortem Procedures for Wildlife Veterinarians and Field Biologists. Paris, France: Office International des Epizooties, 2000.
Wyneken, Jeanette. The Anatomy of Sea Turtles. Dec. 2001. 10 June 2008 <http://courses.science.fau.edu/~jwyneken/sta/>.