Pathophysiology of neuromuscular junction · Normal Neuromuscular Junction Structure. Normal...
Transcript of Pathophysiology of neuromuscular junction · Normal Neuromuscular Junction Structure. Normal...
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Pathophysiology of neuromuscular junction
Assist.Prof.Dr.Jintanaporn WattanathornDepartment of Physiology,
Faculty of Medicine, Khon Kaen University
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Scope
• Autoimmunity mechanism• Normal neuromuscular transmission
process• Effect of antibody on neuromuscular
transmission process
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Myasthenia gravis (MG)
• A condition where voluntary muscles become easily fatigued (tired) and weak (especially muscle that work all the times; 6 muscles that move eye ball and muscle that hold the eye lid).
• A fault in the way nerve messages are passed from the nerves to the muscles.
• Due to a problem with the immune system ( acquired immunological disorder and genetic abnormalities).
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Myasthenia gravis (MG)
• The prevalence of MG in the US ranges from 0.5-14.2 per 100,000 people. The prevalence has increased over the past 2decades.
• Female has more early onset (~30 years), than male (~50 years).
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How immune system creates autoantibody ?
• Toxic B-cell which could harm body’s own tissue escape the detection and destruction during fetal development. (McCalester University )
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How immune system creates autoantibody ?
Infection can trigger.
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How immune system creates autoantibody ?
Abnormal myoid cells in thymus.
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How immune system creates autoantibody ?
Involve MHC class II
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Normal Neuromuscular Junction Structure
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Normal neuromuscular junctions
Esterase stain
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Muscle Nicotinic Receptor
• a gated receptor channel and intrinsic membrane glycoprotein of molecular weight 290,000, =
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• Antigen-presenting cells internalize the antigen (acetylcholine receptor), process it, and then present the processed peptides in association with major histocompatibility complex (MHC) class II molecules unique to the subject. The T-cell receptor of antigen-specific helper T cells (CD4+) binds to the specific MHC-peptide complex. The interaction of the antigen-presenting cell and the T cell requires additional costimulatory signals and is aided by adhesion molecules and cytokines, resulting in T-cell stimulation. The activated T cell helps acetylcholine-receptor-specific B cells. These B cells bind the antigen (acetylcholinereceptor) to their surface antibodies, process it, and present the MHC-peptide complex, like other antigen-presenting cells. They thus interact with T cells by binding to the T-cell receptor. The T cell provides help to the B cells by means of surface molecules and cytokines (not shown), resulting in B-cell proliferation and the secretion of acetylcholine-receptor-specific antibody.
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Modulation of post-synaptic AChRs by anti-AChR antibodies
Anti-AChR antibodies cross-link post-synaptic AChRs
Anti-AChR antibodies cross-link post-synaptic
AChRs
Internalized AChRs are degraded
Fewer AChRs remain on the post-synaptic membrane
Less efficient neuromuscular transmission
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Damage to Post-synaptic Membrane
Complement binds to the Antibody-AChR complex
Membrane-attack complex (MAC)forms on the membrane
The post-junctional membrane is damaged.
- Fewer post-synaptic membrane folds
- Reduced numbers of AChRs
- Widened synaptic cleft
Less efficient neuromuscular transmission
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NEUROMUSCULAR JUNCTION ANATOMY:NORMAL & MYASTHENIA GRAVIS
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Summary
Immune disorder
Nicotinic receptor
Musk
Decrease efficiency of
neuromuscular transmission
Decrease efficiency of
muscle contraction
Muscle weakness
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