Partial recovery Lost sample 1,000 fmols 0.1 fmols 1.0 fmols Neg. control Supplemental Fig. 1....
-
Upload
vernon-preston -
Category
Documents
-
view
217 -
download
0
Transcript of Partial recovery Lost sample 1,000 fmols 0.1 fmols 1.0 fmols Neg. control Supplemental Fig. 1....
![Page 1: Partial recovery Lost sample 1,000 fmols 0.1 fmols 1.0 fmols Neg. control Supplemental Fig. 1. Quality control for extraction procedures using spiked-in.](https://reader036.fdocuments.us/reader036/viewer/2022062322/5697bfbe1a28abf838ca2ddc/html5/thumbnails/1.jpg)
Partial recovery
Lost sample
1,000 fmols
0.1 fmols
1.0 fmols
Neg. control
Supplemental Fig. 1. Quality control for extraction procedures using spiked-in miRNAs from C. elegans. Only qRT-PCR pictures assessed for cell-miR-39 are shown.
Total recovery
Neg. control
Samples
![Page 2: Partial recovery Lost sample 1,000 fmols 0.1 fmols 1.0 fmols Neg. control Supplemental Fig. 1. Quality control for extraction procedures using spiked-in.](https://reader036.fdocuments.us/reader036/viewer/2022062322/5697bfbe1a28abf838ca2ddc/html5/thumbnails/2.jpg)
Supplemental Fig. 2. Distribution of miRNA expression based on the quality after removing all miRNAs with Ct values ≥35 and cluster analysis heatmap plot according to the quality of all determinations. A slightly tendency of the majority of morbid obese (BMI>40 kg/m2) men expressing higher number of miRNAs with a Ct value below 35 Ct was reported. NW (green): Nomalweight, OW (yellow): Overweight, Ob (orange): Obesity, and MOb (red): Morbid obesity. * (violet) indicates subjects with type 2 diabetes.
![Page 3: Partial recovery Lost sample 1,000 fmols 0.1 fmols 1.0 fmols Neg. control Supplemental Fig. 1. Quality control for extraction procedures using spiked-in.](https://reader036.fdocuments.us/reader036/viewer/2022062322/5697bfbe1a28abf838ca2ddc/html5/thumbnails/3.jpg)
Supplemental Fig. 3. Distribution of miRNA expression based on the quality after removing all miRNAs with Ct values ≥35, and cluster analysis heatmap plot according to the quality of all determinations. The tendency of the morbid obese (BMI>40 kg/m2) subjects expressing higher number of miRNAs with a Ct value below 35 Ct before surgery weight-loss (red) was also shown.
![Page 4: Partial recovery Lost sample 1,000 fmols 0.1 fmols 1.0 fmols Neg. control Supplemental Fig. 1. Quality control for extraction procedures using spiked-in.](https://reader036.fdocuments.us/reader036/viewer/2022062322/5697bfbe1a28abf838ca2ddc/html5/thumbnails/4.jpg)
Supplemental Fig. 4. Gene expression stability measure (M, which is the mean pair-wise variation for a gene from all other tested control genes) calculated using the applet GeNorm according to the number of reference miRNAs.
![Page 5: Partial recovery Lost sample 1,000 fmols 0.1 fmols 1.0 fmols Neg. control Supplemental Fig. 1. Quality control for extraction procedures using spiked-in.](https://reader036.fdocuments.us/reader036/viewer/2022062322/5697bfbe1a28abf838ca2ddc/html5/thumbnails/5.jpg)
r=0.44p<0.0001
r=0.37p<0.0001
r=0.42p<0.0001
r=-0.41p<0.0001
r=-0.42p<0.0001
r=-0.44p<0.0001
r=-0.57p<0.0001
r=-0.32P=0.004
Supplemental Fig. 5. Scatter plot representing for the whole cohort (n=80) the correlation between the normalized expression values (even Log2-normalized relative values, or the anti-Log2-transformed DeltaCps values of the means) for (A) miR-142-3p, (B) miR-140-5p, (C) miR-222, (D) miR-221, (E) miR-15a, (F) miR-520c-3p, (G) miR-423-5p, and (H) miR-130b. Values for non-obese men (the reference group) are represented as empty circles (○); obese (30≤ BMI< 40 kg/m2) subjects are represented as empty diamonds (◊); and morbid obese (BMI≥ 40 kg/m2) patients as filled diamonds ().
![Page 6: Partial recovery Lost sample 1,000 fmols 0.1 fmols 1.0 fmols Neg. control Supplemental Fig. 1. Quality control for extraction procedures using spiked-in.](https://reader036.fdocuments.us/reader036/viewer/2022062322/5697bfbe1a28abf838ca2ddc/html5/thumbnails/6.jpg)
Supplemental Fig. 6. Hierarchical clustering (Ward’s criterion) deduced from the qRT-PCR data using independent hydrolysis probes and miRNAs profiles depicting genomic relatedness among the subjects of the whole cohort. NW (green): Normoweight, OW (yellow): Overweight, Ob (orange): Obesity, and MOb (red): Morbid obesity. * (violet) indicates subjects with type 2 diabetes.