PART-I: General Informationdbtepromis.gov.in/Documents/TechnicalDetails/ProposalID_5994_03582… ·...

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PART-I: General Information

1. Name of the Institute/University/Organization submitting the Project Proposal

: CSIR-Central Food Technological Research Institute, Mysore – 570020.

2. State : Karnataka

3. Status of Institute : National Food Research Institute

4. Name and Designation of Executive Authority of the Institute/University forwarding the Application

: Dr. G.Venkateswara Rao Acting Director Central Food Technological Research Institute, Mysore – 570020

5. Project Title : Composite processed fruit products

for nutrients and nutraceuticals

6. Category of the Project : R & D

7. Specific Area : Food Biotechnology

8. Duration : Three years

9. Total Cost (Rs.) : Rs. 198.71 lakhs*

10. Is the Project Single/Institutional/Multi-Institutional

: Single Institution(Multi-departmental)

*That includes Rs.70.10 lakhs equipments to supplement the pilot plant, for

pilot scale demonstration of processes

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11. Project Summary

Fruits contribute to the supplementary diet of human population, with

accessory nutrient factors exerting health values, (such as carotenoids, ascorbic acid,

pigments, polysaccharides, soluble and insoluble fibre ,minerals, phenolics,

flavoniods, terpenes and so on),based on a spectrum of chemical constituents so

characteristic of each species/variety. A meticulous combination of selected isolated

fractions from such of the selected fruits like species of citrus, banana, papaya, mango

and mangosteen, for a selective combination of sets of identified bio-functional

compounds as supplementary and complimentary to daily human requirements, based

on rationale of sensory score to develop liquid, solid and semisolid processed

products, with extended storage life would be the focus of the proposed project.

Experiments to retain ascorbic acid whilst processing ,akin to respective fresh fruit

further with added daily recommended doses ,and to obtain processed products to

contain xanthones ,selected flavonoids ,amino acids ,phenolics, glycosides,

polysaccharides, glycopeptides, terpenoids and inorganic ions, for acceptable texture,

structure ,flavor and taste would be the target . Each of the products would be labeled

for its special and vital property responsible compounds originating from the fruit

(pulp/juice/seed/peel/rind etc.,) parts. This would involve knowledge of chemistry and

[processing for diverse range of products, underlined by the impacting study of

retention, detention, transformation or loss of the target compounds, during

processing, and storage, so that the final processed products would be a composite of

factors contributed by fruits not available throughout the year in all geographic parts of

the world. Accordingly, the project proposal would involve work components specially

addressed by a Food research Institute and accordingly this proposal from CFTRI that

has completed sixty years of its service to the mankind.

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The proposal involves methods to prevent loss/degradation of ascorbic

acid, carotenoids, and anthocyanins during processing.

Keywords: Fruits, bio-functional, complimentary, supplementary, ascorbic acid,

xanthones, carotenoids, anthocyanins, polysaccharides, fibre, flavonoids,

terpenes, processing, value addition.

PART-II: Particulars of Investigators

12.a:

Name : Dr. S. Rajarathnam

Date of Birth : 04-03-1953

Sex : Male

Indicate whether Principal Investigator/Co-Investigator

: Principal Investigator

Designation : Chief Scientist , Head of the Department

Department : Department of Fruit and Vegetable Technology

Institute/University : Central Food Technological Research Institute

Address : Department of Fruit and Vegetable Technology, CFTRI, Mysore-570020, Karnataka.

Phone No. & e-mail

: 91-0821-2515653

[email protected]

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[email protected]

[email protected]

No. of Projects being handled at present

: 4

b:

Name

:

Dr. (Mrs). M.N. Shashirekha


Sex : Female


: Co-Principal Investigator

Designation : Principal Scientist

Department : Department of Fruit and Vegetable Technology


Address : Department of Fruit and Vegetable Technology, CFTRI, Mysore-570020, Karnataka.

Phone No. & e-mail

: 91-0821-2515653

shashirekha_urs@yahoo. co.in


: 3

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c: Name : Dr. L. Jagan Mohan Rao


Sex : Male


: Co-Principal Investigator

Designation : Senior Principal Scientist

Department : Plantation products, spices and flavour Technology


Address : Department of plantation products, spices and flavour technology, CFTRI, Mysore-570020, Karnataka.

Phone No. & e-mail

: 0821 – 2512352

[email protected]


: 3

d : Name Dr. (Mrs.) Shylaja M. Dharmesh

Date of Birth 01-06-1957

Sex Female

Indicate whether Principal Co-Principal Investigator

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Investigator/Co-Investigator

Designation Principal Scientist

Department Department of Biochemistry and Nutrition

Institute/University Central Food Technological Research Institute

Address Department of Biochemistry and Nutrition, CFTRI, Mysore-570020, Karnataka.

Phone No. & e-mail

0821-2514876

[email protected]


3

mailto:[email protected]

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PART-III: Technical Details of the Project (Under the following heads on separate sheet)

16. Introduction

India is like a sub-continent, with great variations in agro-climatic

conditions and accordingly capable of producing a wide range of fruits. Mostly, they

are seasonal, may be tropical, subtropical or temperate. India is the second largest

producer of fruits in the world, next only to China with estimated production figures of

75 mt (FAO 2012).India is the largest producer of mango and banana, estimated

production figures of 16.34 and 31.89 mt known for their exotic varieties of excellent

taste. Species of citrus and grapes account for > 6 mt, whereas pomegranate

production is fast picking up, along with existing pineapple cultivation for yield of ~

2mt. India currently has recorded production of ~ 4.71 mt of papaya, ranking first

place in world(FAO,2012).Besides, the country is known for production of apple

,fig,cherry,cashew,sapota,guava,annonas,carambola,mangosteen etc.,in relatively

lesser quantities ,in certain areas and seasons only.

However, currently food consumption is viewed from the angle of

health benefits, and fruits and vegetables in turn are emphasized since they serve

to supply accessory factors to cereal diets ,supplementing and complementing a

great variety of minerals ,vitamins,fibre,phenolics ,anthocyanins ,flavonoids

,xanthones ,citrus compounds ,carotenoids ,betalains and so on. Fruits and

vegetables due to their high moisture content and faster rate of metabolic activity are

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highly perishable. About 25% of the fresh produce is estimated as under loss every

year, equivalent to a loss of ~ Rs. 25,000 crores. Even conservation of the loss @ 1

% would amount to saving of Rs.250 crores.

To meet daily requirements, it is a combination of very many of the

individual nutrients in defined proportions. Accordingly, the fruit sources vary in

relation to their nutrient /nutraceutical contents and processing into definite products

derived necessary to meet the needs of populations bereft of producing such fruits

and even otherwise due to seasonal production. This would ensure availability of

processed products alround the year. This takes us to study the influence of

processing conditions on the retention, detention, and transformation of bioactive

compounds. Storage stability also needs definition. It is along these lines, the current

project proposal is programmed to supply a said course of nutrients per day to

supplement the cereal diet, from fruits. Five products are finally projected for

formulations, definition of body requiring nutrients /nutraceuticals, taste, influence of

processing and storage stability followed by details of cost of production.

16.1 Origin of the Proposal

India leads in the world production of mango, banana and papaya with

estimated production figures of 16, 32 and 4.7 million tonnes, sharing ~ 40, 32 and

25% of the world production (FAO, 2012). Still, the exports of fruits and vegetables in

fresh and processed form are 20.72 and 8.27 lakh metric tonnes respectively for

earning of Rs.3855 and 3500 crores (2010-2011). Thus, these figures are

proportionately very low and there is considerable scope to raise these values.

Further, different fruits are produced in different geographic parts of the country and

in different seasons. Each fruit and specifically some fruits are known for specific

biofunctional compounds and accordingly, to a resident of any geographic place in a

place, availability of a set of fruits of health value is not a possibility. Processing

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them into products would extend storage life, however, to ensure retention of target

compounds is seemingly a very very difficult task. It is in this context, present

project proposal is programmed to meet these demands and requirements. This

would make trade also economical for domestic / overseas. It also serves to

improve the economic status of rural populations, for producing quality raw-

materials. The urban sectors play the role of primary and secondary processing,

with involved scope for increased employment and revenue generation through

domestic and foreign trade. This has a main emphasis on making available a set of

nutrients, from a combination of various fruits as per prescribed FAO guidelines, with

defined labels of compounds of nutraceutical value that makes a person able to

access his desired nutrients / nutraceuticals, at one given stretch, irrespective of

season and place of production.

16.2 Definition of the Problem

Fruit contributes to the supplementary diet of human population, with

accessibility of nutrient /nutraceutical factors[exerting health values such as

carotenoids, ascorbic acid, pigments, polysaccharides, fibre(both soluble and

insoluble],minerals, phenolics, flavoniods, terpenes , synephrine, neodiosmin and so

on),based on a spectrum of chemical constituents so characteristic of each

species/variety. A meticulous combination of selected isolated fractions from such of

the selected fruits like species of citrus, banana, papaya, mango and mangosteen,

based on rationale of sensory score to develop liquid, solid and semisolid processed

products, with extended storage life would be the focus of the proposed project. Each

of the products would be labeled for its special and vital property responsible

compounds originating from the fruit (pulp/juice/seed/peel/rind etc.) parts. This would

involve rich knowledge of chemistry and [processing for diverse range of products,

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underlined by the impacting study of retention, detention, transformation or loss of the

target compounds, during processing, and storage. Thus, the project proposal would

involve work components specially addressed by a Food research Institute and

accordingly this proposal from CFTRI that has completed sixty years of its service to

the mankind.

To retain vitamin C, carotenoids and anthocyanins despite processing

conditions, formulations to comprise a set of bio-functional compounds, at

concentrations recommended for nutri /nutra values.

16.3 Objectives

1. Study of specific varieties of fruits of citrus, banana, mango, papaya, and

mangosteen, for their specific bio-chemical and bio-functional constituents

(from pulp, peel, seed, rind etc.).

Identification of suitable varieties of fruits for target compounds in

relation to display of bio-functional properties.

2. Food formulations based on sensorially compatible fruit products along

with recommended nutrients and nutraceuticals derived from fruit bases.

Processed food formulations to contain bio-functional components for

a study of influence of processing conditions.

3. Study of storage life of the processed composite fruit products for

microbiological and bio-functional qualities, with a final labeling of the key

chemical components.

Pilot scale studies to work out the cost economics for preparation of

Detailed Project report (DPR) for technology transfer.

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16.7 Preliminary work done so far:

a) Components of 70% ethanolic extract of mangosteen fruit rind (~62% of fruit

as inedible residue) are studied for their phenolics, both free & bound.

b) Bio-functional properties of naringin, hesperidin and limonin of citrus species

are evaluated.

17. Review of the current status of Research and Development in the subject

17.1 International status

Various components of fruits are known for different bio-functional

properties.

Fresh fruits in general are a source of ascorbic acid .Due to the role of

ascorbate in protecting cells against oxidative stress and the involvement of ROS in

neurodegenerative disorders (Alzheimer’s and Parkinson’s diseases) or inflammatory

response (arteriosclerosis), it is strongly suggested that vitamin C could prevent heart,

chronic inflammatory, and neurodegenerative diseases . Vitamin E (tocopherols and

tocotrienols) is present in all cell membranes and plasma lipoproteins, especially in

red blood cells of the human body. As the major lipid-soluble chain-breaking

antioxidants in humans, vitamin E protects DNA, low-density lipoproteins, and

polyunsaturated fatty acids from oxidative damage.Chlorogenic acid and caffeic acid

inhibit N-nitrosation reaction and prevent the formation of mutagenic and carcinogenic

N-nitrosocompounds. Polyphenols have been acknowledged to have health beneficial

effects, owing to derived products such as flavonoids, tannins, coumarins and lignans

(Lee et.al, 2010). Carotenoid-rich diets are correlated with a significant reduction in

the risk for certain cancers, coronary heart disease, and several degenerative

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diseases. Carotenoids have demonstrated anticancer and anti-mutagenic properties.

Some sterols are provitamins upon skin exposure to UV radiation, they may give rise

to calciferol, also known as vitamin D2, which is involved in the absorption of calcium

and bone growth. Plant sterols possess, moreover, cholesterol-lowering properties

and play a positive role by decreasing the incidence of cardiovascular diseases.

Being structurally similar to cholesterol, they can compete with cholesterol, thus

limiting its absorption from fat matrices into the intestinal tract. Plant sterols have been

hypothesized to have anticancer, anti-atherosclerosis, anti-inflammation, and

antioxidant activities (Patil et.al. 2009).

A reduction in the prevalence of certain forms of cancer has been

attributed to the anti-carcinogenic properties of certain glucosinolates and their

breakdown products. Glucosinolates act by activating enzymes involved in the

detoxification of carcinogens and by providing protection against oxidative damage.

Certain glucosinolates have been observed to inhibit enzymes involved in the

metabolism of steroid hormones (Poiroux-Gonord et. al., 2010). Resveratrol has been

identified as one of the major active compounds of stilbenes found in grapes, grape

products, crane berries, and strawberries. Positive effects of resveratrol on health

include the prevention of cell oxidation through resveratrol antioxidant properties ,

ability to stop pain, stop the growth of bacteria that cause stomach ulcers that can lead

to cancer, protect immune cells, protect DNA, protect skin cancer, as well as being

cardio-protective, antitumor, estrogenic, anti-platelet and anti-inflammatory

actions(Tosun and Inkaya , 2010). Major natural antioxidants found in fruits and

vegetables may be used in promoting cardio-health. Since different food categories

possess different bioactive compounds with various antioxidant capacities, specific

foods, when consumed together, may produce synergistic antioxidant interactions and

in turn have more positive physiological effect on cardio-health than when consumed

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alone (Wang et. al., 2011). Ethanol extract of F.religiosa exerted hypolipidemic and

antioxidant effects (Hamed, 2011). Work to identify potential sources of bioactive

compounds through the determination of flavonoids and ellagic acid contents and the

in vitro anti oxidant capacity and -glucocidase and -amylase inhibitory activities of

Brazilian native fruits and commercial frozen pulps is reported (Goncalves et. al.,

2010).

Antioxidant activity is proportionate to the content of flavonoids and

proliferation inhibition ability is proportionate to the content of both flavonoids and

limonoids (Patil et.al. 2009). Ripe papaya fruit contained 132-166 μg retinol

equivalents per 100 g of fresh weight (Schweiggert et. al., 2012).Stage of ripeness

significantly influences the contents of bioactive compounds in papaya fruit (Gayosso-

Garcia Sancho et .al. 2011). Naringin was the predominant flavanone in pummelo

varieties (Zhang et. al., 2011). Phenolic rich fractions were condensed tannins with

structural heterogeneity in monomer units, degree of polymerization and interflavan

linkages in mangosteen fruit rind with condensed tannins predominately that contain

procyanidins with a significant amount of propelargonidin (Zhou et. al., 2011). Fibre

rich powder from Mangifera pajang peels is a rich source of dietary fibres, antioxidants

and other bioactive compounds that can be incorporated with food products to

improve the nutraceutical properties of these products (Hassan et. al., 2011).The

tested phenolics in Phyllanthus emblica fruit showed strong radical scavenging

activity, good potency to chelate Fe2+ and good inhibition ability of lipid peroxidation

(Luo et. al., 2011). Mango fruit fiber is a source of phenolics,carotenoids,minerals and

fibre both soluble and insoluble ,with sufficient flavor ,for development of bakery

products.

The increase in number of scientific papers published for the last

two decades correlating diet and some chronic diseases has shown extraordinary

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possibilities of foods to support, or even to improve, our health. As a consequence,

nowadays, there is a huge interest among consumers and food industries on products

that can promote health and well being. These foods have been genetically named

“functional foods” (Plaza et.al. 2008).

Large number of classes and representative examples of

compounds from various segments of foods are being investigated for their possible

role in human health. Behaviour of compounds on isolation, and in situ along with

others could differ and this needs detailed study. In vitro assessments need to be

followed by animal experiments and final confirmation comes out by feeding to human

subjects. Accordingly, the series of study is quite long and worth undertaking

.Eventually, it is a combination of different classes of compounds related to the food

sources, whose consumption should yield to the benefits of health. Thus, it is a

synergy of vitamins, minerals, flavonoids, phenolics, pigments, peptides

polysaccharides, fibre (soluble and insoluble) and so on, geared towards human

health. Influence of processing conditions on the retention, detention, and

transformation of the target compounds is worthwhile exploring further. All these

indicate promising future and evidently confer to the health benefits of components of

dietary foods (Rajarathnam et.al.2012).

17.2 National Status

Several laboratories are processing fruits and vegetables for routine

products like beverage, jam, jelly or dehydrated. This has little or no emphasis on

active ingredients that each fruit is known for. Particularly the effect of processing

conditions and storage period influence vividly and very distinctly retention

/transformation of the target nutrients / bio-compounds. Whilst requiring to combine a

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range of compounds, it is necessary to combine a range of fruits, with needed extra

supplements, to mold definite and precise products subjected to various processing

conditions. This would involve primary processing, secondary processing, storage,

and cost of production /selling price for cost economics. This would serve to reduce

the field perishability, ensure the farmer suitable returns, promote production by the

masses than production enmasse.The present project proposal is programmed along

these lines to benefit the farmers, processors, marketers and consumers.

However references dealing with some of the biofunctional properties

including cytoprotectivity from mango peel are reported (Ajila et.al. 2007). Use of

mango fruit peel powder for preparation of macaroni (Ajila and Prasada Rao, 2010)

and biscuits (Ajila et.al. 2008) is worked out. Functional properties of banana

pseudostem and rhizome are studied (Saravana, 2011).

17.3 Importance of the proposed project in the context of current status

Anticipated products and processes of practical/technological

utility / socio-economic relevance expected to be evolved by pursuing the

project

India is mainly and basically known for its agriculture, wherein fruit

production is a means of earning to the thousands of farmers. Due to great variations

in agro-climatic conditions, because of geographical differences, India is like a sub-

continent, wherein great diverse range of fruit crops is produced. India represents the

second largest producer of fruits in the world with estimated figures of 75 million

tonnes; however, its processing is only about 3% and export trade is <10%. Thus,

there is ample scope to identify, collect, pre-treat, primary process followed by

secondary processing, with a focus on bio-functional constituents, to reach reduction

in bulk and ready to consume products, to serve transportation and trade over

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distance and time. This would ensure also availability of such of these fruit products

to such of those geographic areas of world bereft of the fruit under question. Thus,

eventually, this cycle of events centers a) around the farmer / producer to raise his

economic standards through ensured selling at reasonable rates and b) surrounds the

urban processing industries to cater to the needs of populations looking for ready to

consume, bio-functional processed compact products with extended storage life, for

trade even outside the country despite the increased costs and reasonable value

addition. Streamlining the step – wise operations, would greatly impact the producers,

processors and the consumers. This essentially underlines the improvement,

betterment and growth of country’s economy.

17.4 Expertise available with the proposed investigating group/institution in

the subject of the project.

a) Dr. S. Rajarathnam, serving as Head of the Department of Fruit and

Vegetable Technology. Planning and co-ordination of the R & D projects of the

departmental staff, for presentation to the Research Advisory Committee, Sponsoring

agency, budget sanction, expenditure, co-ordination and monitoring of research for

results, compilation of data into completion reports and annual reports.

b)Dr. (Mrs.) M.N. Shashirekha, senior scientist of the Fruit and Vegetable

Technology with specialization in the field of fruit and vegetable post-harvest

technology and processing for storage. Serving to undertake research on chemistry,

bio-chemistry, and enzyme systems of fruits and vegetables. Processing of fruits and

vegetables into products both primary and secondary.

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c)Dr. L. Jagan Mohan Rao, senior scientist with specialization in organic and

structural chemistry for isolation, chromatography and spectral data on identification

of compounds of bio-functional value.

d)Dr. (Mrs.) Shylaja M. Dharmesh, senior scientist with high specialization in

evaluating biological materials for the bio-functional properties, working with

biological systems to enumerate behavior of the compounds isolated or in situ, to

define health benefits.

18. Work Plan

18.1 Methodology

Proprietary / Patented items, if any expected to be used for this project.

1. Processing of selected fruits and their isolated fractions in combinations to label

the products specifically for the identified biofunctional constituents, and

product formulations and optimization of processing conditions to retain the

target biofunctional components, their confirmation through chromatographic

studies.

2. Definition of storage life of products based on microbiological and chemical

qualities and bio-functional properties.

3. Scale-up studies of at least five products in pilot plant and details of cost

economics and DPR preparation.

Definition of the isolated fruit fractions for a set of identified bio-functional

properties and influence of processing conditions would form the alternate strategy, to

develop value added products for retention of target compounds.

.

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18.2 Organization of Work elements

1. S.Rajarathnam: Planning, co-ordination, chemistry, and influence of

processing conditions on retention of target compounds.

2. M.N.Shashirekha: Execution, chemistry of selected fruits for their

components, formulations and processing.

3. L. Jagan Mohan Rao: Chromatography, MS data interpretation

4. Shylaja M.Dharmesh: Bio-functional activities of target compounds in select

fruits.

Eventually, by the end of project period, at least five products

would be released with five processes, with DPR.

18.3 Suggested Plan of action for utilization of research outcome expected

from the project

Basic / Applied Research outcome:

Research on extraction, fractionation and characterization of target of

components from formulations of selected fruits / their parts would cover the basic

aspects of investigation that would be published, in renowned journals. So also

establishment of biofunctional properties of the fruit constituents, particularly after

incorporation into processed products falls under basic research. These aspects

would be covered under Ph.D. works of the three research fellows to be appointed

in the project. Product formulations, processing conditions followed by scale up

work in pilot plant with product labeling and storage studies cover the applied

research.

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Thus, the project proposal would encounter both basic and applied research,

qualifying the three RFs for Ph.D degree on one hand and release of 5 novel

processed biofunctional labeled products to the market (domestic / overseas). Non-

obvious research findings would be covered under IPR, for filing the patents.

Outcome:

The proposed project proposal would focus on the development of processed

products from selected fruits (from pulp/juice, peel, seed and rind) in meticulous

compatible acceptable sensorial combinations, to ensure the retention of target

compounds of bio-functional importance, during processing and storage. This

would be of great help and aid to benefit the growers /farmers to look for diversion

of their produce for transformed forms of products for extended storage life and

value addition. This would be marketed over distance and time, and thus favors

overseas trade too. Accordingly, the proposal serves to benefit both the rural

sectors of fruit production and the urban sectors of processing and consumption.

At least five products of bio-functional value, with extended storage life and

value addition would be evolved during this investigation, as something different

and as “Newer introductions into the market”.

18.5 Time schedule of activities giving milestones

6 months: Isolation of fruit fractions of bio-functional importance.

12 months: Bio-functional properties of isolated fractions.

18 months: Formulations of processed products from select fractions of select

fruits.

24 months: Influence of processing conditions on retention of target

compounds.

30 months: Pilot scale studies of select five processed products.

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36 months: Details of cost economics for preparation of “Detailed Project

Report”, for release of technology.

18.6 Project implementing agency

S. No.

Name of the Agency Address of the agency

Proposed research aspect

Proposed amount

Cost sharing

1. Department of Biotechnology, Govt. of India, New Delhi

Secretary DBT, Ministry of Science & Technology, New Delhi

As in the format

Rs.128.61 lakhs

100%

2. CFTRI, Mysore Director, CFTRI, Mysore

As in the format

– –

PART-IV: Budget Particulars

19. Budget (Rs. in lakhs)

A. Non- recurring (e.g. equipments, accessories, etc.)*

Sl. No.

Items Year-1 Year-2 Year-3 Total

1. Flash evaporator 6.00 6.00

2. Lyophiliser 8.00 8.00

3. HPLC columns (3 No.)

2.40 2.40

4. Ice Making Machine 0.20 0.20

5. GLC/GCChromatographic Columns(3 Types)

2.40 2.40

6. Fraction collector 6.00 6.00

7. Fibre Estimation Unit 8.00 8.00

8. Spcetrophotometer with monitor /Digital Printer

8.00 8.00

9. Deep Freezers (3 No.) 1.50 1.50

10. Incubators (2 No.) 1.00 1.00

11. Oven (2 No.) 0.80 0.80

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12. HPLC 18.00 18.00

13. Spares & Installation Accessories 1.50 1.50

14. Speedvac 6.00 6.00

15. Western Blotting Equipment 6.00 6.00

Sub Total –A1 75.80

Justification:

A. Non-recurring (Equipments):

1. Flash-evaporator: To concentrate the fruit extractives and derivatives for

chromatography and characterization of compounds.

2. Lyophilizer: To freeze dry the samples for analysis.

3. HPLC-columns: To aid in chromatography of fruit extractives.

4. Ice-making machine: To ensure supply of easy ice to undertake chemical

and biochemical reactions of analysis and chromatography.

5. GLC-GC-Chromatographic columns: To serve chromatographic analysis of

derivatized samples.

6. Fraction collector: To aid fractionation of target fruit extractives on ion

exchange / molecular sieve/resin chromatography.

7. Fibre estimation unit: To serve the estimation of dietary fibre (total, soluble

and insoluble) in large number of samples of raw-material and processed

products.

8. Spectrophotometer with minitor / digital printer: To regularly monitor the

chemical reactions, estimations of chemical constituents and enzyme assays.

9. Deep freezers: To store fruit samples / extractives and derivatives till the final

transformations.

10. Incubators: For chemical estimations and microbiological works.

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11. Ovens: For chemical estimations and microbiological works.

12. HPLC: Basic requirement for all chromatographic analyses of samples.

13. Spares and installation accessories: UPS, stabilizers etc,. to

serve efficient and long standing use of the equipments projected.

14. Speedvac: For concentrating low yielding biological components from

cells/tissues.

15. Western Blotting Equipment: For the precise identification of bio-markers.

List of equipments proposed for pilot scale demonstration of processes*

Sl.No Name of the equipment Estimated Cost (Rs. in Lakhs)

1. Pulper 0.60

2. Juice extractor 0.75

3. Hot air drier 4.00

4. Thermo sealer of plastic cups 2.00

5. Spray drier 21.00

6. Fruit disintegrator 1.00

7. Water activity meter 3.00

8. Moisture reader 2.00

9. Centrifuge 10.00

10. Colloidal mill 1.50

11. Laminated aluminium pouch automatic sealer

2.00

12. Laminated aluminium pouch making unit(automatic)

1.00

13. Walk-in-cooler 7.00

14. Establishment costs (@ 25%) 14.00

Total –A2 : 70.10

Total (A1+A2)=Rs.145.90Lakhs

*Equipments to supplement the pilot plant, for pilot scale demonstration

of the processes developed, for a net estimatd cost of ~ Rs.70.10 lakhs.

(Budgetary quotations are awaited).

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B. Recurring

B.1 Manpower (Rs. in Lakhs)

Sl. No.

Position No. Consolidated emoluments

Year-1 Year-2 Year-3 Total

1. Senior Research Fellow (Three No.)

7.776 7.776 8.864 24.416

Sub total (B.1) 24.416

Justification: Three SRFs: They are required to carry out the fruit extractions,

chemical analyses, chromatography and bio-functional properties of the products.

Eventually, they can register for their Ph.D degrees.

Sl. No.

Items Year-1 Year-2 Year-3 Total

1. Raw materials, chemicals (Rough and fine) and glass wares

3.00 3.50 3.50 10.00

Sub total (B.2) 10.00

Other Item Qty. Year-1 Year-2 Year-3 Total

B.3 Travel 1.50 1.50 1.50 4.50

B.4 Contingency

2.00 2.50 2.50 7.00

Sub Total– (B.3 + B.4) 11.50

Total – (B.1+B.2+B.3+B.4) 45.916

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Justification:

C. Recurring:

1. Consumables: Towards chemicals (rough and fine chemicals),including

solvents for extraction of biofunctional compounds; for chromatography, NMR

and MS studies.

Sigma standards for identification and authentication, glass wares

for chemical work and columns for fractionation of fruit extractives.

2. Manpower: Three SRFs as per DBT/CSIR norms. They are required to carry out

the fruit extractions, chromatography and biofunctional properties of the products.

Eventually, they can register for their Ph.D degrees.

3. Travel: Towards procurement of selected varieties of fruits, even from other

states, in desired physiological state, for extraction purposes. To attend

symposia / seminars and vital meetings. Presentations of progress report to

DBT, at intervals, as desired by DBT.

4. Contingencies: Towards small gadgets and minor works to ensure completion

of the projected plan of work. Contract labour as inevitable for scale up studies in

pilot plant etc. Towards procurement of essential text books, and meet page

charges for publishing in reputed research journals.

C. CFTRI component

Institutional charges @ 15% of recurring budget (Rs. 45.92 lakhs) = 6.89 --C

D. Grand Total (Rs.): 145.90(A1+A2) +45.92(B) +6.89(C) =198.71 Lakhs

PART-V: Existing Facilities

20. Available facilities as accessories to be utilized for the project

S. No.

Name of the equipment/accessories

Make Model Funding Agency

Year of Procurement

1. Pilot plant for extraction and fruit processing

- - CSIR 2002

2. Boiler for steam supply - CSIR 2002

3. Weighing Balances Shimadzu CSIR 2010

4. Texturo meter CSIR 2005

25

5. Color reading Meter CSIR 2006

6. Electronic Nose CSIR 2005

7. Atomic Absorption Spectrophotometer & Inductively Coupled Plasma

CSIR 2005

8. Water Activity Meter CSIR 2011

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26

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27

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Food Science and Nutrition (Accepted).

28

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Declaration / Certification

It is certified that

(a) The research work proposed in the scheme/project does not in any way duplicate the

work already done or being carried out elsewhere on the subject.

(b) The same project has not been submitted to any other agency/agencies for financial

support.

(c) The emoluments for the manpower proposed are those admissible to persons of

corresponding status employed in the institute/university or as per the Ministry of

Science & Technology guidelines (Annexure-III).

(d) Necessary provision for the scheme/project will be made in the Institute/

University/State budget in anticipation of the sanction of the scheme/project.

(e) If the project involves the utilization of genetically engineered organism, it is agreed

that we will ensure that an application will be submitted through our Institutional

Biosafety Committee and we will declare that while conducting experiments, the

Biosafety Guidelines of the Department of Biotechnology would be followed in toto.

(f) If the project involves field trials/experiments/exchange of specimens, etc. we will

ensure that ethical clearances would be taken from concerned ethical

Committees/Competent authorities and the same would be conveyed to the

Department of Biotechnology before implementing the project.

(g) It is agreed that any research outcome or intellectual property rights(s) on the

invention(s) arising out of the project shall be taken in accordance with the instructions

issued with the approval of the Ministry of Finance, Department of Expenditure, as

contained in Annexure-V.

(h) We agree to accept the terms and conditions as enclosed in Annexure-IV. The same is

signed and enclosed.

33

Bio-data of Principal Investigator

Name: Dr. S. Rajarathnam Designation: Chief Scientist – IV (6); Head of the Department Department: Fruit and Vegetable Technology Institute: Central Food Technological Research Institute Address: Cheluvamba Mansion, KRS Road, MYSORE – 570020 Telephone: 0821 – 2515653 (Office) 0821-2442230(Res) Fax: 0821 – 2517233 E-mail: [email protected]

[email protected]. [email protected] Date of Birth: 4 March 1953 Sex: Male Educational Qualifications:

University Degree Year Division / Class / Grade

University of Mysore

B.Sc. 1971 First Class*

" M.Sc. (Botany)

1973 First Class**

" Ph.D.(Applied Botany)

1982 -

* Represented “University First” from the faculties of Biological Sciences, from the University of Mysore. * * a. was awarded subject scholarship in M.Sc. (Previous) b. Secured Fifth Rank in M.Sc. (Final) Guide ship recognized: a) Biochemistry (University of Mysore)[Current No. of Students:1 b) Biotechnology (University of Mysore) [Current No. of students: 2

Ph.D Thesis: “Studies on pathological and biochemical aspects during cultivation and storage of the white oystermushroom,Pleurotus flabellatus”(Guide:Dr.M.V.Patwardhan)

Research Experience: 38 Years

Patents: 24 Technologies Developed: 24 Publications:

a) Research Papers - 41

b) Reviews Presented at Symposia - 26

c) International Reviews/Chapter for Text Books - 20

d) Popular Article -1

60



34

Fields of Research Experience:

1. Post-harvest technology for extension of storage life of fresh fruits and vegetables.

2. Processing and development of value added products from fruits,

vegetables and mushrooms. 3. Chemistry and Bio-Chemistry of mushrooms, fruits and vegetables.

4. Large scale mushroom production and processing.

5. Nutraceuticals from fruits, vegetables and mushrooms.

Awards:

1. Received the “National Technology Award” by the Ministry of Science

and Technology, New Delhi, on 11th May 2003 for contribution to the

development and transfer of technology on “Large scale production of

organic quality mushrooms and processed products for domestic and

export trade”.

2. Awarded the First Prize for poster presentation on “Avenues for processing

of custard apple”, in the IFCON 2003 held during 5-8th Dec. 2003 at

CFTRI, Mysore.

3. Best Technology Transfer Award for the year 2011 (CFTRI Award) for

the technology transfer of “Preparation of bio-functional beverage and jelly

from sweet orange” to M/s.VTR Company, Bangalore, on exclusive basis (3

years)for Rs.10 lakhs.

Nominations

1. Maniammai – Periar University, Tanjore – serving as member of Governing Council.

2. IIHR, Bangalore -Served as RC member, for evaluation of Annual Progress Report.

3.Tezpur University, Assam (Central University)-- Expert member of the committee for the selection of faculty for Food Science Technology Department.

4. National committee on “Artificial ripening agents for fruit ripening”. Recognitions

1. Based on the database inquiries, selected as a “Referee” to the American Chemical Society (USA) and British Mycological Society (UK).

2. Selected at the world level for contributions of two articles on “Mushrooms” in Encyclopedia of Food Science and Nutrition,

35

Academic Press, U.S.A. published once in ten years (10 Volumes costing Rs 1,00,000/-).

Others a) Serving as Referee to Department of Biotechnology and Department

of Science and Technology, DSIR and CSIR, New Delhi for evaluating the project proposals, for financial support.

b) Serving as a member of Bureau of Indian Standards, New Delhi. c) Serving as editor to the Journal of Food Science and Technology

(India) d) Worked as Principal investigator and Co-principal investigator of

Department of Biotechnology (New Delhi) sponsored projects on Mushroom Science and Technology since 1993.

e) As Principal Investigator of several other projects on fruits and vegetables.

Deputation Abroad:

Presentation of paper on papaya in symposium at Brazil ---- 31st

October to 4th November 2011“Perspectives of processing papaya fruit: National

and International Strategies’’ at Brazil “The Papaya Brazil 2011: V Brazilian

Symposium on Papaya”.

List of Publications

I. Research Papers

1. Kavishree, S., Hemavathy, J., Lokesh, B.R., Shashirekha, M.N., and Rajarathnam, S.

(2007) Fat and Fatty acids of Indian edible mushrooms. Food Chemistry, 106 (2) :

597-602

2. Shashirekha,M.N., and Rajarathnam,S.(2007) Bioconversion and biotransformation of

coir pith for economic production of Pleurotus florida: chemical and biochemical

changes in coir pith during the mushroom growth and fructification. World Journal of

Microbiology and Biotechnology, 23: 1107-1114

3. Shashirekha, M.N., Revathy Baskaran, Jaganmohan Rao, L., Vijayalakshmi,M.R. and

Rajarathnam,S. (2008) Influence of processing conditions on flavor compounds of

custard apple (Annona squamosa,L).LWT-Food Science and Technology (UK),

41(2): 236-243.

4. Nethravathi,G.P.,Sathisha,U.V.,Shylaja,M.Dharmesh,Shashirekha,M.N.,and

Rajarathnam,S. (2006) Anti-oxidant Activity of Indigenous Edible Mushrooms. Journal

of Agricultural and Food Chemistry, 54: 9764-9772.

5. Rajarathnam,S., Shashirekha,M.N. and Zakia Bano (2005) Influence of supplementing

the rice straw growth substrate with cotton seeds, on the analytical characteristics of

the mushroom, Pleurotus florida. Food Chemistry [U.K.], 92: 255-259

36

6. Rajarathnam,S., Shashirekha,M.N. and Rashmi (2003) Biochemical changes

associated with mushroom browning in Agaricus bisporus and Pleurotus florida :

Commercial implications. Journal of the Science of Food and Agriculture [U.K.],

83: 1531-1537

7. Rajarathnam,S., Shashirekha,M.N., and Zakia Bano (2001) Biodegradation of

gossypol by the white oyster mushroom, Pleurotus florida, during culturing on rice

straw growth substrate, supplemented with cottonseed powder. World Journal of

Microbiology and Biotechnology (Netherlands), 17: 221 - 227.

8. Shashirekha,M.N., Rajarathnam,S., and Zakia Bano (2001) Chemical and biochemical

changes in the rice straw substrate related to the morphogenesis, cropping pattern and

yield of Pleurotus florida (Block & Tsao). Journal of Horticultural Science and

Biotechnology (U.K), 76(3): 332 - 337.

9. Shashirekha,M.N., Rajarathnam,S., and Zakia Bano (2001) Enhancement of

bioconversion efficiency and chemistry of the mushroom, Pleurotus sajor-caju (Berk &

Br.) Sacc. produced on spent rice straw substrate, supplemented with the oil seed

cakes. Food Chemistry (U.K) 76: 27– 31.

10. Zakia Bano, Shashirekha,M.N., and Rajarathnam, S (1993) Improvement of the

bioconversion and biotransformation efficiencies of the oyster mushroom (Pleurotus

sajorcaju) by supplementation of its rice straw substrate with oilseed cakes. Enzyme

Microbial Technology (U.K), 15: 985-989.

11. Rajarathnam, S., Shashirekha,M.N., and Zakia Bano (1992) Inhibition of growth of

Pleurotus flabellatus (Berk. & Br.) Sacc. on rice straw by Sclerotium rolfsii.

Scientia Horticulturae (Netherlands), 51: 295-302

12. Rajarathnam,S., Zakia Bano, Berger,R.G., and Drawert,F (1990) Effect of

amendment of the growth substrate on the flavour composition of fruit bodies of

Pleurotus flabellatus. Chemie Microbiologie Technologie er Lebensmittel (West

Germany), 12:145-150.

13. Rajarathnam,S., Wankhede,D.B. and Zakia Bano (1987) Biotechnological

implications of degradation of rice straw degraded by Pleurotus flabellatus

Journal of Chemical Technology and Biotechnology (U.K), 37: 203-214.

14. Zakia Bano, Rajarathnam, S. and Narasimha Murthy,K (1986) Studies on the fitness

of “Spent straw” obtained during cultivation of the mushroom Pleurotus sajor-caju for

safe consumption as cattle feed. Mushroom Newsletter for the Tropics (Hong

Kong), 6(3): 11-16.

15. Zakia Bano and Rajarathnam,S. (1986) Vitamin values of Pleurotus mushrooms.

Qualitas Plantarum : Plant Foods for Human Nutrition (Netherlands), 36: 11-15.

37

16. Rajarathnam,S., Zakia Bano and Patwardhan,M.V. (1985) Nutrition of the mushroom

Pleurotus flabellatus during its growth on paddy straw substrate. The Journal of

Horticultural Science (U.K),61:223-232.

17. Rajarathnam,S., Zakia Bano and Narasimham,P. (1984) In vitro evaluation of some

fungicides to control Sclerotium rolfsii contamination during cultivation of the

mushroom, Pleurotus flabellatus (Berk & Br.) Sacc. Mushroom Newsletter for the

Tropics (Hong Kong), 5(2): 7-11.

18. Rajarathnam,S., Zakia Bano and Patwardhan,M.V. (1983) Post-harvest

physiology and storage of the white Oyster mushroom, Pleurotus flabellatus.

Journal of Food Technology (U.K),18: 153-162.

19. Rajarathnam,S., Zakia Bano and Singh,N.S. (1983) Efficacy of formaldehyde and

liquor ammonia for controlling the growth of Sclerotium rolfsii during cultivation

of the mushroom Pleurotus flabellatus. Mushroom Newsletter for the Tropics

(Hong Kong), 3(4): 3-10.

20. Zakia Bano and Rajarathnam, S (1982) Studies on the cultivation of Pleurotus sajor-

caju. The Mushroom Journal (U.K), 115: 243-245.

21. Zakia Bano, Rajarathnam,S and Muthu,M. (1981) Use of ethyl formate in

controlling the growth of Sclerotium rolfsii during cultivation of Pleurotus species.

Mushroom Science (Australia), XI(2): 541-549.

22. Chandrashekar,T.R., Zakia Bano and Rajarathnam,S. (1981) Incompatibility and

growth in Pleurotus flabellatus. Transactions of the British Mycological Society

(U.K), 77: 491-495.

23. Rajarathnam,S., Singh,N.S. and Zakia Bano (1979) Efficacy of carboxin and heat

treatment for controlling the growth of Sclerotium rolfsii during culture of the

mushroom Pleurotus flabellatus. Annals of Applied Biology (U.K), 92: 323-328.

24. Rajarathnam,S., Wankhede,D.B. and Patwardhan,M.V. (1979) Some chemical and

biochemical changes in straw constituents during the growth of Pleurotus flabellatus

(Berk & Br.) Sacc. European Journal of Applied Microbiology and Biotechnology

(West Germany), 8: 125-134.

25. Zakia Bano, Nagaraja,N., Rajarathnam,S. and Patwardhan,M.V. (1979) Cultivation of

Pleurotus species in a village model hut. Indian Food Packer XXXIII (6): 19-25.

26. Zakia Bano, Rajarathnam,S. and Nagaraja,N. (1978). Some aspects on the cultivation

of Pleurotus flabellatus in India. Mushroom Science (France), X (2): 597-608.

27. Rajarathnam,S., Zakia Bano and Muthu,M. (1977) Studies on the control of

Sclerotium rolfsii Sacc. contamination during the cultivation of Pleurotus flabellatus

(Berk and Br.) Sacc., efficacy of methyl bromide. The Mushroom Journal, (U.K), No.

57: 294-298.

38

28. Singh,N.S. and Rajarathnam,S. (1977) Pleurotus eous (Berk) Sacc. A new cultivated

mushroom. Current Science (India), 46: 617-618.

II. Research Papers (In Process)

29. Rajarathnam,S., Zakia Bano and Shashirekha,M.N. Observations on the growth

and in vitro degradation of rice straw by fungal contaminations found in beds of the mushroom Pleurotus flabellatus ; ecological considerations. Annals of Applied Biology (U.K).

30. Shashirekha,M.N., Rajarathnam,S., Rashmi and Meera,M.S. Oxygen and carbon-di-

oxide changes in the package atmosphere of Pleurotus florida under different storage conditions. International Journal of Food Science and Technology [U.K.].

31. Shashirekha,M.N., Rajarathnam,S., Meera,M.S. and Rashmi .Influence of factors on changes in reflectance of Agaricus bisporus and Pleurotus florida during storage. Journal of Food Science [U.S.A.].

32. Meera,M.S., Shashirekha,M.N., Rajarathnam,S., and Varadaraj,M.C. Microbiological quality of Agaricus bisporus and Pleurotus florida under different conditions of storage. Food Microbiology [U.K.].

33. Rajarathnam,S., Shashirekha,M.N. and Zakia Bano .CP-MAS C13 NMR of rice straw and its isolated fractions (celulose, hemicellulose and lignin) during biodegradation by Pleurotus florida. Journal of Chemical Technology and Biotechnology (U.K.)

34. Shashirekha,M.N., Rajarathnam,S. and Zakia Bano Effect of supplementing rice straw substrate with cotton seed fractions on the formation of fruiting primordia, yield and protein content of Pleurotus florida. World Journal of Microbiology and Biotechnology (U.K.).

35. Shashirekha,M.N., Rajarathnam,S. and Zakia Bano Pre-treatment of coffee pulp for economic bio-conversion by Pleurotus florida. Enzyme Microbial Technology (U.K.).

36. Mallikarjuna SE, Mahadevamma M, Shashirekha MN and Rajarathnam S. Carbohydrate composition of the mushrooms, Lentinula edodes. Food Chemistry.

37. Sowbhagya, Shashirekha MN and Rajarathnam S. Pigments from mangosteen rind. Journal of Science, Food and Agriculture.

38. Mallikarjuna SE, Murlikrishna, G, Shashirekha MN and Rajarathnam S. Physicochemical characteristics of water soluble polysaccharides from Lentinula edodes. Carbohydrate Research.

39. Shivleela,V.B., Shashirekha,M.N., Jagan Mohan Rao, L and Rajarathnam, S. Physicochemical properties of xanthones isolated from Mangosteen rind. Journal of Science, Food and Agriculture.

40. Shivleela,V.B., Shashirekha,M.N., Shylaja Dharmesh, Rajarathnam, S. Biochemical properties of Mangosteen rind and beverage therefrom. Food Chemistry.

39

41. Shivleela,V.B., Shashirekha,M.N., Shylaja Dharmesh, Rajarathnam, S. Biochemical changes and biofunctional properties of sweet orange (Citrus sinensis) juice during debittering. Journal of Science, Food and Agriculture.

III. Research Reviews Presented at the Symposia

1. Shivaleela,V.B , Shashirekha,M.N, and Rajarathnam,S. Chemistry of apple pomace

for food applications. Society Biological Chemists 80th Annual Meeting, November

12th- 15th 2011, CI-MAP, Lucknow.

2. Pavithra K., Mallikarjuna.S.E., Shashirekha.M.N and Rajarathnam.S .Physico-

chemical characterization of Alkali-soluble fraction- I of Mucilage from cladode of

Opuntia dillenii. Society Biological Chemists 80th Annual Meeting, November 12th- 15th

2011, CI-MAP, Lucknow.

3. Chinmayee, Pavithra,K. Haware D. J . Shashirekha,M.N. and Rajarathnam,S . Minerals of cactus (Opuntia dilleni). Society Biological Chemists 80th Annual Meeting,

November 12th- 15th 2011, CI-MAP, Lucknow.

4. Mallikarjuna S.E., Shivaleela V.B., Shashirekha M.N. and Rajarathnam

S.(2011)Antioxidant effects of artificially grown mushroom fruiting bodies of Lentinula

edodes and Pleurotus florida. 52nd Annual Conference of Association of

Microbiologists of India (AMI), 3rd NOV – 6th NOV 2011 Chandigarh.

5. Mallikarjuna SE, Mahadevamma M, Sukumar Debnath, Lokesh BR,Shashirekha MN

and Rajarathnam S (2009). Carbohydrate and lipid composition of the mushrooms

Lentinula edodes and L. cladopus. 20th Indian Convention of Food Scientists &

Technologists(ICFOST-2009), 21-23, Dec. 2009, held at NIMANS, Bangalore, India,p-

166.

6. Ranjini A, Devendra J Haware, Mallikarjuna SE, Vijayalakshmi MR,Shashirekha MN

and Rajarathnam S (2009). Mineral composition of species of Lentinula (Shiitake)

compared to species of Pleurotus(Oyster) mushrooms. 20th Indian Convention of Food

Scientists& Technologists(ICFOST-2009), 21-23, Dec. 2009, held at NIMANS,

Bangalore, India,p-167.

7. Shashirekha,M.N., Vijayalakshmi,M.R., Revathy Baskaran and Rajarathnam,S.

“Processing of low cost pear fruit into value added products, free of discoloration and

merited nutri / nutra values”. IFCON-2008, Dec 15-19, 2008, Mysore.

8. Shashirekha, M.N., Netravathi, G.P., and Rajarathnam, S. Nutritional and

nutraceutical evaluation of indigenous edible mushrooms. Poster presentation at the

International Conference on“Biotechnology Approaches for Alleviating Malnutrition

and Human health”. 9-11th January 2006, at UAS, Bangalore.

9. Thejaswini, H.B., Tharanathan, R.N., Mahadevamma, S., Shashirekha, M.N., and

Rajarathnam, S. Polysaccharides from Calocybe indica: Physico-chemical

characteristics For Poster presentation at 75th Annual SBC Conference, New Delhi, 8-

11th December 2006.

40

10. Srunga Narayana., Srinivas, P., Shashirekha, M.N., and Rajarathnam, S., Phenolics

of the mushroom, C tropicalis. For Poster presentation at 75th Annual SBC

Conferencee, New Delhi, 8-11th December 2006.

11. Nethravathi, G.P., Sathisha, U.V., Shylaja, M. Dharmesh, Shashirekha,M.N. and

Rajarathnam, S. Antioxidant activity of indigenous edible mushrooms. Poster

presentation at ICFOST 2005 (December 2005, at CFTRI, Mysore).

12. Kavishree,S., Hemavathy,J., Lokesh, B.R..,Shashirekha, M.N., and

Rajarathnam,S.,(2004) Studies on lipids of indigenous edible mushrooms.ICFOST-

2005.(Poster presentation).

13. Rajarathnam,S. (2004) Naturally growing mushrooms of the tribal belts of India :A

diverse range of species for diversified applications.(Invited Lecture, at ICFOST-

2004).

14. Vijayalakshmi M.R., Revathy Baskaran, Iboyiama singh, N., Shashirekha M.N.,

Rajarathnam,S. (2003) Avenues for processing custard apple. 5th International Food

Convention, 5-8 December 2003, India (Poster presentation).

15. Manoj Kumar Verma, Vijayalakshmi, M.R., and Rajarathnam, S. (2003) Colour

stability in grape (Bangalore grape variety) products during processing and storage.

5th International Food Convention, 5-8 December 2003, India (Poster presentation).

16. Rajarathnam,S., Zakia Bano and Shashirekha,M.N. (2000) Mushrooms : An item of

food delicacy or Health conditioner. In: Microbial Biotechnology for Sustainable

Development and Productivity (Ed., R.C. Rajak) pp : 140 - 151, Scientific

Publishers, Jodhpur.

17. Rajarathnam,S., Shashirekha, M.N., and Zakia Bano (1997) Renewable

ligninocellulosic wastes – the growth substrates for mushroom production: national

strategies. pp. 291 – 304. Advances in Mushroom Biology and Production.

Mushroom Society of India. NCMRT, Solan.

18. Zakia Bano, Rajarathnam, S. and Shashirekha, M.N. (1997) Post-harvest physiology,

quality and storage of fresh mushrooms. 7pp 321 – 338. Advances in Mushroom

Biology and Production. Mushroom Society of India. NCMRT, Solan.

19. Zakia Bano, and Rajarathnam,S. (1994). Mushroom Production. In: Specialist Group

Meeting and Symposium on Solid State Fermentation", 23-24 March 1994,

Trivandrum, Seminar on Mushroom Cultivation organized by Agricultural Finance

Department, Central Office, Indian Bank, Madras (India), July 1987.

20. Zakia Bano and Rajarathnam, S. (1994) Mushroom Processing: Future Perspective.

In: "National Symposium on Mushrooms",8-10 April 1994, Solan (Himachal

Pradesh).

41

21. Zakia Bano, and Rajarathnam, S. (1994) Mushrooms - Human Nutrition and Health.

In : Microbes for better living. MICON - 94, 35th AMI Cong., 9-12, November 1994.

395-399.

22. Zakia Bano, Rajarathnam,S. (1989). Bioconversion of straw by oyster mushroom.

Fibrous Crop Residues as Animal Feed, ICAR (India), 57-65.

23. Rajarathnam,S. and Zakia Bano (1987).Biological significance of the natural

lignocellulosic wastes degraded by Pleurotus mushrooms. Indian Mushroom

Science (India) II, 296-304.

24. Zakia Bano, Rajarathnam,S. and Nagaraja, N. (1987). Some important studies on

Pleurotus mushroom technology. Indian Mushroom Science (India), II, 53-64.

25. Rajarathnam,S. and Zakia Bano (1987). Culturing and processing of oyster

mushrooms a.

26. Zakia Bano and Rajarathnam,S. (1987). Prospects of Pleurotus technology in India a.

aSeminar on Mushroom Cultivation organized by Agricultural Finance Department,

Central Office, Indian Bank, Madras (India), July 1987.

IV. International Reviews/Chapter for Text Book

1. Rajarathnam, S., and Shashirekha, M.N. (2003). Mushrooms and Truffles:

Classification and morphology. Encyclopedia in Food Science and Nutrition,

Academic Press, (U.K.) pp4040-4048.

2. Rajarathnam, S., and Shashirekha, M.N. (2003). Mushrooms and Truffles: Use of Wild

Mushrooms. Encyclopedia in Food Science and Nutrition, Academic Press, (U.K.)

pp4048-4054.

3. Rajarathnam,S. Shashirekha,M.N. and Zakia Bano (1998). Biodegradative and

biosynthetic capacities of mushrooms: Present and future strategies. Critical Reviews

in Biotechnology (U.S.A), 18(2&3): 91-236.

4. Zakia Bano, Shashirekha, M. N and Rajarathnam,S. (1996) Biotransformation

effeciencies of ligno-cellulose wastes by mushroom in solid state fermentation.

Journal of Scientific and Industrial Research, 55 : 400-407.

5. Zakia Bano, Rajarathnam,S. and Shashirekha,M.N. (1993) Production and

processing of mushrooms. Food Digest, 16(1), 61-69.

6. Rajarathnam,S. Shashirekha,M.N. and Zakia Bano.(1992) Biopotentialities of the

Basidiomacromycetes, In: Advances in Applied Microbiology, Academic Press Inc.,

California (U.S.A), 37, 234-361.

42

7. Zakia Bano, Rajarathnam,S. and Shashirekha,M.N. (1992) Mushrooms. The

unconventional single cell protein for a conventional consumption. Indian Food

Packer, 46(6), 20-31.

8. Rajarathnam,S.and Zakia Bano.(1990) Biological utilization of the fruiting fungi. In:

Advances in Applied Mycology, Marcel Dekker Press. Inc., New York (U.S.A), 3,

241-392.

9. Rajarathnam,S. and Zakia Bano.(1989) Pleurotus mushrooms. Part III.

Biotransformation of natural lignocellulosic wastes: Commercial applications and

implications. CRC Critical Reviews in Food Science and Nutrition (U.S.A), 28 (1),

31-113.

10. Rajarathnam,S. and Zakia Bano (1988) Pleurotus mushrooms. Part IA. Morphology,

Life Cycle, Taxonomy, Breeding and Cultivation. CRC Critical Reviews in Food

Science and Nutrition (U.S.A), 26(2), 157-223.

11. Rajarathnam,S. and Zakia Bano.(1988) Pleurotus mushrooms. Part IB.Pathology, in

vitro and in vivo growth requirements, and world status. CRC Critical Reviews in

Food Science and Nutrition (U.S.A), 26(3), 243-311.

12. Zakia Bano and Rajarathnam,S. (1988) Pleurotus mushrooms. Part II. Chemical

composition, nutritional value, post-harvest physiology, preservation and role as

human food. CRC Critical Reviews in Food Science and Nutrition (U.S.A), 27(2),

87-158.

13. Zakia Bano and Rajarathnam. S. (1982) Pleurotus mushrooms as a Nutritious Food.

In: Tropical Mushrooms, Biological Nature and Cultivation Methods", (Hong

Kong), Edited by S.T.Chang and T.H.Quimio, Chinese University Press.

Text book on “Advances in fruit and vegetable technologies” (that had sought

Director’s kind approval), with nineteen chapters, edited,(S.Rajarathnam &

R.S.Ramteke) Published by M/s. New India Publishing Agency, New Delhi(with six

chapters):

14. Rajarathnam, S. Introduction and scope for fruit and vegetable technologies

15. Rajarathnam S. Influence of processing conditions on the quality of processed

products.

16. Rajarathnam S. Further considerations in fruit and vegetable technologies

17. Shashirekha M.N &. Rajarathnam ,S. Storage and processing of mushrooms

18. Vijayalakshmi, M.R. & Rajarathnam, S .Analytical methods in processing of fruits and

vegetables .

19. Shashirekha, M.N. & Rajarathnam, S. Mushroom Nutraceuticals .

20. Rajarathnam S., Shashirekha M.N. and Mallikarjuna , SE. (2012). Status of

bioactive compounds in foods, with focus on fruits and vegetables. Critical Reviews in

Food Science and Nutrition (Accepted).

43

V. Popular Article

1. Contributed to the formation of the book “The Great Indian Mushrooms" (1992), for wide circulation, printed and published on behalf of Ministry of Food Processing Industries, Govt. of India, (20,000 copies for distribution).

VI. Patents

1. A process for the preparation of custard apple jam and a jam thus obtained.

Inventors: M.R.Vijayalakshmi, MN. Shashirekha S. Rajarathnam and Revathy

B,Patent No.115193 granted by Registry of patents Singapore 30.4.08; 225674

dt.21-3-03.

2. A process for the preparation of fruit mix from custard apple. Revathy Baskaran,

S. Rajarathnam, M.N. Shashirekha and M.R. Vijayalakshmi.383/DEL/03

dt.26-3-03.

3. A process for the preparation of dehydrated product from custard apple

Inventors: S. Rajarathnam MN. Shashirekha, M.R.Vijayalakshmi and

Revathy B. Indian Patent no. 221088, Issue Date: 2008-08-01.

4. A process for the preparation of cereal flakes from custard apple.

M.R.Vijayalakshmi ,S. Rajarathnam, M.N. Shashirekha, Revathy Baskaran.

215690 dt.27-2-08.

5. A process for the preparation of nectar from custard apple. S. Rajarathnam,

M.N. Shashirekha, M.R. Vijayalakshmi,and Revathy Baskaran.221087

dt.17-6-08.

6. A process for the preparation of jelly from custard apple. M.N. Shashirekha,

S.Rajarathnam, M.R. Vijayalakshmi and Revathy Baskaran.240503 dt.13-5-10.

7. A process for the preparation of shelf stable pulp from custard apple.

Inventors: MN. Shashirekha, S. Rajarathnam , M.R.Vijayalakshmi, and Revathy

B . Patent No. 14141 Patented by SriLanka 13.7.09.236660 dt.16-11-08.

8. Custard apple powder and a method for preparation. Revathy Baskaran,

M.R. Vijayalakshmi , M.N. Shashirekha and S. Rajarathnam.465/DEL/04 ,

dt.31-10-05.

44

9. Ready to serve beverage from custard apple by Microfiltration.

N.ISingh,M.R.Vijayalakshmi, M.N.Shashirekha, Revathy Baskaran and S.

Rajarathnam No.1-2002-00225 dated 30.7.10 patented by Republic of

Phillipines. 193548 dt.13-1-06.

10. An improved process for the storage of fresh mushrooms. M.N. Shashirekha,

S. Rajarathnam, M.C. Varadaraj and K.R. Kumar.222012 dt.15-7-08.

11. An improved device for economic production of an edible fungus. S.

Rajarathnam, Zakia Bano and M.N. Shashirekha.220756 dt. 4-6-08.

12. A simple process for organic production of an edible fungus. S.Rajarathnam,

Zakia Bano and M.N. Shashirekha.226 /DEL/02 dt.14-3-02.

13. A process for the extension of storage life of fresh custard apple fruits by surface

coating with cactus mucilage. M.N.Shashirekha and S.

Rajarathnam.433/DEL/10 dt.26-2-10.

14. A process for herbal induced ripening of banana. M.N.Shashirekha,

M.N.Keshavaprakash, B.S.Sridhar, R.S.Matche and

S.Rajarathnam.2438/DEL/10 dt.13-10-10.

15. A process for preparation of beverage and jelly from mangosteen fruit rind .M.N.

Shashirekha, Shylaja M. Dharmesh, and S. Rajarathnam.2934 /DEL/11

dt.12-10-11.

16. A process for the preparation of bio-functional beverage from sweet orange

(Citrus sinensis) M.N. Shashirekha, Shylaja M. Dharmesh , and S.

Rajarathnam.(NF 0006) under process.

45

VII. Technology / Process / Product development:

a) List of processes:

SI.No. Title Authors

1. Large scale production of oyster

mushrooms on coir pith

S. Rajarathnam, Zakia Bano

M.N. Shashirekha


mushrooms-Urban model


M.N. Shashirekha


mushrooms-Rural model


M.N. Shashirekha

4. Process for oyster mushroom

dehydration.


M.N. Shashirekha

5. Oyster mushroom-Pickle & sweet

chutney.


M.N. Shashirekha

6. Mushroom canning S. Rajarathnam, Zakia Bano

M.N. Shashirekha


mushrooms on coffee pulp


M.N. Shashirekha.

8. Mushroom production by using

substrate supplemented with cotton seed

meal

S. Rajarathnam,Zakia Bano

M.N. Shashirekha

9. Shelf stable custard apple pulp M.N. Shashirekha

S. Rajarathnam

M.R. Vijayalakshmi

Revathy Baskaran and Ramesh M.N.

10. RTS beverage from micro-filtered

custard apple juice

Iboyiama singh,

M.R. Vijayalakshmi

M.N. Ramesh

Revathy Baskaran

M.N. Shashirekha

S. Rajarathnam

11. Instant mushroom soup mix S. Rajarathnam

Zakia Bano

M.N. Shashirekha and Ramesh, M.N.

12. Custard apple Jam, Jelly and fruit mix M.R. Vijayalakshmi

M.N. Shashirekha

Revathy Baskaran

M.N. Ramesh

S. Rajarathnam

13. Debittering of kinnow orange juice for

preparation of concentrate and

sweetened juice

S.K. Berry, Seghal, Shashirekha,

M.N., Revathy Bhaskaran, K.V.R.

Ramana, Rajarathnam,

S.,Venkatesh Murthy, Mohan Lal,

Vijayalakshmi, M.R, Asha

15. Process for preparation of product from Pear-dehydrated

M.N. Shashirekha, M.R. Vijayalakshmi, Revathy Bhaskaran, M.R. Asha, P.C.S. Nambiar and S. Rajarathnam

46

16. Process for preparation of product from Pear-Juice

M.R. Vijayalakshmi, M.N., Shashirekha, Revathy Bhaskaran, M.R. Asha, P.C.S. Nambiar and S. Rajarathnam

17. Process for preparation of product from Pear-Powder

Revathy Bhaskaran, M.R. Vijayalakshmi, M.N. Shashirekha, M.R. Asha, P.C.S. Nambiar and S. Rajarathnam

18. A process for herbal ripening of banana

M.N. Shashirekha,L.Jagan Mohan

Rao, M.N.Keshav Prakash,

B.S.Sridhar and S. Rajarathnam

19. A process for development of cereal flakes and spray dried powder from custard apple.

M.R. Vijayalakshmi, Revathy

Baskaran , M.N. Shashirekha, Umesh

Hebbar and S. Rajarathnam

20. A process for development of instant broccoli soup mix.

M.N. Shashirekha, M.R.

Vijayalakshmi, Revathy Baskaran

and S. Rajarathnam

21. Process for the extension of storage life of custard apple

M.N. Shashirekha, Umesh Hebbar and S. Rajarathnam.

22.

Process know-how for the preparation of blended beverages from banana pseudostem juice

M.R. Vijayalakshmi, P.C. S. Nambiar and S. Rajarathnam

23. A process for preparation of beverage and jelly from sweet orange

(Citrus sinensis) fruit

M.N.Shashirekha ,Shylaja M.Dharmesh ,S.Rajarathnam, Umesh Hebbar, Prem Vishwanath,Maya Prakash,Vijayalakshmi

24. A process for preparation of nutraceutical beverage and jelly from mangosteen rind

M.N.Shashirekha ,Shylaja M.Dharmesh ,S.Rajarathnam, Umesh Hebbar, Maya Prakash

b) List of products developed:

1. Instant natural fruit milk shake powders

2. RTS custard apple beverage

3. Stable ,frozen custard apple pulp.

4 Kinnow orange jelly.

5. Kinnow orange spray dried powder.

6. Instant mushroom soup mix.

7. Texturized banana (at lab scale)

8. Texturized mango (at lab scale)

9. Cereal flakes from kinnow orange

(directly without debittering process)

10. Marmalade from Kinnow orange (directly

without debittering)

11.RTS beverage from kinnow orange


12. Mushroom pickle

13. Mushroom sweet chutney

14. Pear juice

15. Dehydrated broccoli

47

16. Broccoli soup mix

17. RTS banana pseudo stem beverage

18. Mushroom biscuits

19. Mushroom flakes

20. RTS beverage from pear

21.Button Mushroom beverage

22. Pear dehydrated

23. Mangosteen rind beverage

24.Mangosteen rind jelly

25.Sweet orange de-bittered bio-functional beverage

26.Sweet orange de-bittered jelly

27.Bio-functional mushroom jelly

-------------

48

Bio-data of Co-Principal Investigator

Name: Dr. Mrs. M.N. Shashirekha

Designation: Principal Scientist

Department: Fruit and Vegetable Technology

Institute: Central Food Technological Research Institute

Address: Cheluvamba Mansion,

KRS Road

MYSORE – 570020

Telephone: 0821 – 2515653 (Office)

Fax: 0821 – 2517233

E-mail: [email protected]

Date of Birth: 8 May 1958

Sex: Female

Educational Qualifications:

University Degree Year Division /

Class /

Grade

University of

Mysore

B.Sc. 1978 First Class

" M.Sc. 1980 First Class

(III Rank)

" Ph.D. 1988 -

Ph.D Thesis: Studies on the extension of storage life of seed potatoes stored under different temperature conditions Guideship Recognized: Biotecthnology

49

Research Experience: 31 Years Publications:

a) Research Papers - 32

b) Reviews Presented at Symposia - 15

c) International Reviews/Chapter for Text Books - 10

d) Popular Article - 1

Patents: 16 Technologies Developed: 24 Fields of Research Experience:

1. Post-harvest technology for extension of storage life of fresh fruits

and vegetables.

2. Processing and development of value added products from fruits,

vegetables and mushrooms.

3. Chemistry and bio-chemistry of mushrooms, fruits and vegetables.

4. Large scale mushroom production and processing.

5. Nutraceuticals from fruits, vegetables and mushrooms.

Awards:

1. CSIR – JRF(1981-1983),CSIR- SRF(1983-1986),CSIR-RA(1990-

1995) and Scientist Fellow (1996-1997).

2.“National Technology Award” by the Ministry of Science and Technology, New Delhi, on 11th May 2003 for contribution in the development and transfer of technology on “Large scale production of organic quality mushrooms and processed products for domestic and export trade”.

3. First Prize for poster presentation on “Avenues for processing of custard apple”, in the IFCON 2003 held during 5-8th Dec. 2003 at CFTRI, Mysore.

4. Best Technology Transfer Award for the year 2011 (CFTRI Award) for the technology transfer of “Preparation of bio-functional beverage and jelly from sweet orange” to M/s.VTR Company, Bangalore, on exclusive basis (3 years)for Rs.10 lakhs.

Recognitions:

1) Selected at the world level for contributions of two articles on “Mushrooms” in Encyclopedia of Food Science and Nutrition, Academic Press, U.S.A. published once in ten years (10 Volumes costing

60

50

Rs. 1,00,000/- Others:

a) Worked as Co-Investigator, Co-principal Investigator and Principal

investigator of Department of Biotechnology (New Delhi) sponsored projects

since 1997.

b) Served as Sub- Project Leader of MLP component of CFTRI projects on

fruits and vegetables since 2001.

List of Publications

I. Research Papers

1. Shashirekha, M.N., Revathy Baskaran, Jaganmohan Rao, L., Vijayalakshmi,M.R.

and Rajarathnam,S. (2008) Influence of processing conditions on flavor compounds

of custard apple (Annona squamosa,L).LWT-Food Science and Technology (UK),

41(2): 236-243.

2. Kavishree, S., Hemavathy, J., Lokesh, B.R., Shashirekha, M.N., and Rajarathnam,

S. (2007) Fat and Fatty acids of Indian edible mushrooms. Food Chemistry, 106

(2) : 597-602.

3. Shashirekha, M.N., and Rajarathnam,S.(2007) Bioconversion and

biotransformation of coir pith for economic production of Pleurotus florida:

chemical and biochemical changes in coir pith during the mushroom growth and

fructification. World Journal of Microbiology and Biotechnology, 23: 1107-1114.

4. Nethravathi, G.P., Sathisha, U.V., Shylaja,M.Dharmesh, Shashirekha,M.N.,and

Rajarathnam,S. (2006) Anti-oxidant Activity of Indigenous Edible Mushrooms.

Journal of Agricultural and Food Chemistry, 54: 9764-9772.

5. Rajarathnam,S., Shashirekha,M.N. and Zakia Bano (2005) Influence of

supplementing the rice straw growth substrate with cotton seeds, on the analytical

characteristics of the mushroom, Pleurotus florida. Food Chemistry [U.K.], 92:

255-259

6. Rajarathnam,S., Shashirekha,M.N. and Rashmi (2003) Biochemical changes

associated with mushroom browning in Agaricus bisporus and Pleurotus florida :

Commercial implications. Journal of the Science of Food and Agriculture [U.K.],

83: 1531-1537

7. Rajarathnam,S., Shashirekha,M.N., and Zakia Bano (2001) Biodegradation of

gossypol by the white oyster mushroom, Pleurotus florida, during culturing on rice

51

straw growth substrate, supplemented with cottonseed powder. World Journal of

Microbiology and Biotechnology (Netherlands), 17: 221 - 227.

8. Shashirekha,M.N., Rajarathnam,S., and Zakia Bano (2001) Chemical and

biochemical changes in the rice straw substrate related to the morphogenesis,

cropping pattern and yield of Pleurotus florida (Block & Tsao). Journal of

Horticultural Science and Biotechnology (U.K), 76(3): 332 - 337.

9. Shashirekha,M.N., Rajarathnam,S., and Zakia Bano (2001) Enhancement of

bioconversion efficiency and chemistry of the mushroom, Pleurotus sajor-caju (Berk

& Br.) Sacc. produced on spent rice straw substrate, supplemented with the oil

seed cakes. Food Chemistry (U.K) 76: 27– 31.

10. Zakia Bano, Shashirekha,M.N., and Rajarathnam, S (1993) Improvement of the

bioconversion and biotransformation efficiencies of the oyster mushroom (Pleurotus

sajorcaju) by supplementation of its rice straw substrate with oilseed cakes.

Enzyme Microbial Technology (U.K), 15: 985-989.

11. Rajarathnam, S., Shashirekha,M.N., and Zakia Bano (1992) Inhibition of growth of

Pleurotus flabellatus (Berk. & Br.) Sacc. on rice straw by Sclerotium rolfsii.

Scientia Horticulturae (Netherlands), 51: 295-302.

12. Shashirekha, M.N., Ramesh, B.S., and Narasimham. P. (1991). Sprouting behavior of NAA treated seed potato tubers after prolonged storage at low temperature. Asian potato Journal (Indonesia) 2:5-11.

13. Shashirekha, M.N., and Narasimham. P. (1990). Effect of triacontanol on

sprout growth in potatoes (Solanum tuberosum).Journal of Science Food and Agriculture (U.K) 50: 1-7

14. Shashirekha, M.N., and Narasimham. P. (1990). Effect of treating seed

potatoes with trace elements on sprouting and microbial spoilage during storage under tropical conditions. Annals of Applied Biology, (U.K) 117: 645-652.

15. Shashirekha, M.N., and Narasimham. P. (1989). Comparative efficacies of gibberellic acid (GA) and ethrel mixture and other established chemicals for the induction of sprouting in seed potato tubers. Journal of Food Science and Technology, 25 (6): 335-339.

16. Shashirekha, M.N., and Narasimham. P. (1989). Pre-planting treatment of

seed potato tuber pieces to break dormancy, control tuber piece decay and improve yield. Experimental Agriculture (U.K) 25: 27-33.

17. Shashirekha, M.N., Karanth. N.G. K., and Narasimham. P., (1987). Surface

microflora of seed potatoes (Solanum tuberosum,), L.CV. Kufri Jyothi): Isolation and identification of organisms responsible for spoilage of potatoes

52

grown at Devanahalli. Journal of Food Science and Technology 24: 261-263.

18. Shashirekha, M.N., and Narasimham. P. (1987). Inhibitory effect of mercuric

compounds and dyes on decay and sprouting of seed potato tubers during storage. Journal of Food Science and Technology, 24: 225-229.

19. Shashirekha, M.N., Rama. M.V., and Narasimham.P. (1983) Synergistic action of gibberlin and ethrel on the inducement of sprouting in potatoes. Journal of Food Science and Technology, 20:120-122.

II. Research Papers (In Process)

20. Rajarathnam,S., Zakia Bano and Shashirekha,M.N. Observations on the growth and in vitro degradation of rice straw by fungal contaminations found in beds of the mushroom Pleurotus flabellatus ; ecological considerations.

Annals of Applied Biology (U.K).

21. Shashirekha,M.N., Rajarathnam,S., Rashmi and Meera,M.S. Oxygen and carbon-di-oxide changes in the package atmosphere of Pleurotus florida

under different storage conditions. International Journal of Food Science and Technology [U.K.].

22. Shashirekha,M.N., Rajarathnam,S., Meera,M.S. and Rashmi .Influence of factors on changes in reflectance of Agaricus bisporus and Pleurotus florida during storage. Journal of Food Science [U.S.A.].

23. Meera, M.S., Shashirekha,M.N., Rajarathnam,S., and Varadaraj,M.C. Microbiological quality of Agaricus bisporus and Pleurotus florida under different conditions of storage. Food Microbiology [U.K.].

24. Rajarathnam,S., Shashirekha,M.N. and Zakia Bano CP-MAS C13 NMR of rice straw and its isolated fractions (celulose, hemicellulose and lignin) during biodegradation by Pleurotus florida. Journal of Chemical Technology and Biotechnology (U.K.).

25. Shashirekha,M.N., Rajarathnam,S. and Zakia Bano Effect of supplementing

rice straw substrate with cotton seed fractions on the formation of fruiting primordia, yield and protein content of Pleurotus florida. World Journal of Microbiology and Biotechnology (U.K.).

26. Shashirekha,M.N., Rajarathnam,S. and Zakia Bano Pre-treatment of coffee pulp for economic bio-conversion by Pleurotus florida. Enzyme Microbial Technology (U.K.).

27. Mallikarjuna SE, Mahadevamma M, Shashirekha MN and Rajarathnam S. Carbohydrate composition of the mushrooms, Lentinula edodes. Food Chemistry.

53

28. Sowbhagya, Shashirekha MN and Rajarathnam S. Pigments from mangosteen rind. Journal of Science, Food and Agriculture.

29. Mallikarjuna SE, Murlikrishna, G, Shashirekha MN and Rajarathnam S. Physicochemical characteristics of water soluble polysaccharides from Lentinula edodes. Carbohydrate Research.

30. Shivleela,V.B., Shashirekha,M.N., Jagan Mohan Rao, L and Rajarathnam,

S. Physicochemical properties of xanthones isolated from Mangosteen rind. Journal of Science, Food and Agriculture.

31. Shivleela,V.B., Shashirekha,M.N., Shylaja Dharmesh, Rajarathnam, S. Biochemical properties of Mangosteen rind and beverage therefrom. Food Chemistry.

32. Shivleela,V.B., Shashirekha,M.N., Shylaja Dharmesh, Rajarathnam, S. Biochemical changes and biofunctional properties of sweet orange (Citrus sinensis) juice during debittering. Journal of Science, Food and Agriculture.

III. Research Reviews Presented at the Symposia

33. Mallikarjuna SE, Mahadevamma M, Sukumar Debnath, Lokesh BR,

Shashirekha MN and Rajarathnam S (2009). Carbohydrate and lipid

composition of the mushrooms Lentinula edodes and L. cladopus. 20th

Indian Convention of Food Scientists & Technologists(ICFOST-2009), 21-23,

Dec. 2009, held at NIMANS, Bangalore, India,p-166.

34. Ranjini A, Devendra J Haware, Mallikarjuna SE, Vijayalakshmi

MR,Shashirekha MN and Rajarathnam S (2009). Mineral composition of

species of Lentinula (Shiitake) compared to species of Pleurotus(Oyster)

mushrooms. 20th Indian Convention of Food Scientists&

Technologists(ICFOST-2009), 21-23, Dec. 2009, held at NIMANS,

Bangalore, India,p-167.

35. Shashirekha,M.N., Vijayalakshmi,M.R., Revathy Baskaran and

Rajarathnam,S. “Processing of low cost pear fruit into value added products,

free of discoloration and merited nutri / nutra values”. IFCON-2008, Dec 15-

19, 2008, Mysore.

36. Shashirekha, M.N., Netravathi, G.P., and Rajarathnam, S. Nutritional and

nutraceutical evaluation of indigenous edible mushrooms. Poster

54

presentation at the International Conference on“Biotechnology Approaches

for Alleviating Malnutrition and Human health”. 9-11th January 2006, at UAS,

Bangalore.

37. Thejaswini, H.B., Tharanathan, R.N., Mahadevamma, S., Shashirekha,

M.N., and Rajarathnam, S. Polysaccharides from Calocybe indica: Physico-

chemical characteristics For Poster presentation at 75th Annual SBC

Conference, New Delhi, 8-11th December 2006.

38. Srunga Narayana., Srinivas, P., Shashirekha, M.N., and Rajarathnam,

S.,Phenolics of the mushroom, C tropicalis. For Poster presentation at 75th

Annual SBC Conferencee, New Delhi, 8-11th December 2006.

39. Nethravathi, G.P., Sathisha, U.V., Shylaja, M. Dharmesh, Shashirekha,M.N. and

Rajarathnam, S. Antioxidant activity of indigenous edible mushrooms. Poster

presentation at ICFOST 2005 (December 2005, at CFTRI, Mysore).

40. Kavishree,S., Hemavathy,J., Lokesh, B.R..,Shashirekha, M.N., and

Rajarathnam,S.,(2004) Studies on lipids of indigenous edible mushrooms.ICFOST-

2005.(Poster presentation).

41. Vijayalakshmi M.R., Revathy Baskaran, Iboyiama singh, N., Shashirekha M.N.,

Rajarathnam,S. (2003) Avenues for processing custard apple. 5th International Food

Convention, 5-8 December 2003, India (Poster presentation).

42. Rajarathnam,S., Zakia Bano and Shashirekha,M.N. (2000) Mushrooms : An item

of food delicacy or Health conditioner. In: Microbial Biotechnology for

Sustainable Development and Productivity (Ed., R.C. Rajak) pp : 140 - 151,

Scientific Publishers, Jodhpur.

43. Rajarathnam,S., Shashirekha, M.N., and Zakia Bano (1997) Renewable

ligninocellulosic wastes – the growth substrates for mushroom production: national

strategies. pp. 291 – 304. Advances in Mushroom Biology and Production.

Mushroom Society of India. NCMRT, Solan.

44. Zakia Bano, Rajarathnam, S. and Shashirekha, M.N. (1997) Post-harvest

physiology, quality and storage of fresh mushrooms. 7pp 321 – 338. Advances in

Mushroom Biology and Production. Mushroom Society of India. NCMRT, Solan.

45. Shivaleela,V.B , Shashirekha,M.N, and Rajarathnam,S. Chemistry of apple

pomace for food applications. Society Biological Chemists 80th Annual Meeting,

November 12th- 15th 2011, CI-MAP, Lucknow.

46. Pavithra K., Mallikarjuna.S.E., Shashirekha.M.N and Rajarathnam.S .Physico-

chemical characterization of Alkali-soluble fraction- I of Mucilage from cladode of

Opuntia dillenii. Society Biological Chemists 80th Annual Meeting, November 12th-

15th 2011, CI-MAP, Lucknow.

47. Chinmayee, Pavithra,K. Haware D. J . Shashirekha,M.N. and Rajarathnam,S . Minerals of cactus (Opuntia dilleni). Society Biological

55

Chemists 80th Annual Meeting, November 12th- 15th 2011, CI-MAP, Lucknow.

48. Mallikarjuna S.E., Shivaleela V.B., Shashirekha M.N. and Rajarathnam

S.(2011)Antioxidant effects of artificially grown mushroom fruiting bodies of

Lentinula edodes and Pleurotus florida. 52nd Annual Conference of

Association of Microbiologists of India (AMI), 3rd NOV – 6th NOV 2011

Chandigarh.

IV. International Reviews/Chapter for Text Book

49. Rajarathnam, S., and Shashirekha, M.N. (2003). Mushrooms and Truffles:

Classification and morphology. Encyclopedia in Food Science and Nutrition,

Academic Press, (U.K.) pp4040-4048.

50 Rajarathnam, S., and Shashirekha, M.N. (2003). Mushrooms and Truffles: Use of

Wild Mushrooms. Encyclopedia in Food Science and Nutrition, Academic

Press, (U.K.) pp4048-4054.

50. Rajarathnam,S. Shashirekha,M.N. and Zakia Bano (1998). Biodegradative and

biosynthetic capacities of mushrooms: Present and future strategies. Critical

Reviews in Biotechnology (U.S.A), 18(2&3): 91-236.

51.Zakia Bano, Shashirekha, M. N and Rajarathnam,S. (1996) Biotransformation

effeciencies of ligno-cellulose wastes by mushroom in solid state fermentation.

Journal of Scientific and Industrial Research, 55 : 400-407.

52. Zakia Bano, Rajarathnam,S. and Shashirekha,M.N. (1993) Production and

processing of mushrooms. Food Digest, 16(1), 61-69.

53. Rajarathnam,S. Shashirekha,M.N. and Zakia Bano.(1992) Biopotentialities of the

Basidiomacromycetes, In: Advances in Applied Microbiology, Academic Press

Inc., California (U.S.A), 37, 234-361.

54. Zakia Bano, Rajarathnam,S. and Shashirekha,M.N. (1992) Mushrooms. The

unconventional single cell protein for a conventional consumption. Indian Food

Packer, 46(6), 20-31.

55. Shashirekha M.N &. Rajarathnam, S. (2011) Storage and processing of

mushrooms. Chapter-11, pp: 373-414 in Text book on “Advances in fruit and

vegetable technologies” (Eds.S.Rajarathnam & R.S.Ramteke). M/s. New India

Publishing Agency, New Delhi.

56. Shashirekha, M.N. & Rajarathnam, S.(2011) Mushroom Nutraceuticals . Chapter-

17, pp: 605-656. In Text book on “Advances in fruit and vegetable

technologies”(Eds.S.Rajarathnam & R.S.Ramteke).M/s. New India Publishing

Agency, New Delhi.

56

57. Rajarathnam S., Shashirekha M.N. and Mallikarjuna , SE. (2012). Status of

bioactive compounds in foods, with focus on fruits and vegetables. Critical

Reviews in Food Science and Nutrition (Accepted).

V. Popular Article

58. Contributed to the formation of the book “The Great Indian Mushrooms" (1992), for wide circulation, printed and published on behalf of Ministry of Food Processing Industries, Govt. of India, (20,000 copies for distribution).

VI. Patents

1. A process for the preparation of custard apple jam and a jam thus obtained.

M.R.Vijayalakshmi,MN.ShashirekhaS. Rajarathnam and Revathy B,PatentNo.115193

granted by Registry of patents Singapore 30.4.08; 225674

dt.21-3-03.

2. A process for the preparation of fruit mix from custard apple. Revathy Baskaran,

S. Rajarathnam, M.N. Shashirekha and M.R. Vijayalakshmi.383/DEL/03

dt.26-3-03.

3. A process for the preparation of dehydrated product from custard apple

Rajarathnam MN. Shashirekha, M.R.Vijayalakshmi and Revathy B. Indian Patent

no. 221088, Issue Date: 2008-08-01.

4. A process for the preparation of cereal flakes from custard apple.

M.R.Vijayalakshmi ,S. Rajarathnam, M.N. Shashirekha, Revathy Baskaran.

215690 dt.27-2-08.

5. A process for the preparation of nectar from custard apple. S. Rajarathnam,

M.N. Shashirekha, M.R. Vijayalakshmi,and Revathy Baskaran.221087

dt.17-6-08.

6. A process for the preparation of jelly from custard apple. M.N. Shashirekha,

S.Rajarathnam, M.R. Vijayalakshmi and Revathy Baskaran.240503 dt.13-5-10.

7. A process for the preparation of shelf stable pulp from custard apple.

MN. Shashirekha S. Rajarathnam , M.R.Vijayalakshmi, and Revathy B

Patent No. 14141 Patented by SriLanka 13.7.09.236660 dt.16-11-08.

57

8. Custard apple powder and a method for preparation. Revathy Baskaran,

M.R. Vijayalakshmi , M.N. Shashirekha and S. Rajarathnam.465/DEL/04 ,

dt.31-10-05.

9. Ready to serve beverage from custard apple by Microfiltration.

N.ISingh,M.R.Vijayalakshmi, M.N.Shashirekha, Revathy Baskaran and S.

Rajarathnam No.1-2002-00225 dated 30.7.10 patented by Republic of Phillipines.

193548 dt.13-1-06.

10. An improved process for the storage of fresh mushrooms. M.N. Shashirekha,

S. Rajarathnam, M.C. Varadaraj and K.R. Kumar.222012 dt.15-7-08.

11. An improved device for economic production of an edible fungus. S.

Rajarathnam, Zakia Bano and M.N. Shashirekha.220756 dt. 4-6-08.

12. A simple process for organic production of an edible fungus. S.Rajarathnam, Zakia

Bano and M.N. Shashirekha.226 /DEL/02 dt.14-3-02.

13. A process for the extension of storage life of fresh custard apple fruits by surface

coating with cactus mucilage. M.N.Shashirekha and S. Rajarathnam.433/DEL/10

dt.26-2-10.

14. A process for herbal induced ripening of banana. M.N.Shashirekha,

M.N.Keshavaprakash, B.S.Sridhar, R.S.Matche and S.Rajarathnam.2438/DEL/10

dt.13-10-10.

15. A process for preparation of beverage and jelly from mangosteen fruit rind .M.N.

Shashirekha, Shylaja M. Dharmesh, and S. Rajarathnam.2934 /DEL/11

dt.12-10-11.

16. A process for the preparation of bio-functional beverage from sweet orange (Citrus

sinensis) M.N. Shashirekha, Shylaja M. Dharmesh , andS. Rajarathnam.(NF 0006)

under process.

58

VII. Technology / Process / Product development:

a) List of processes:

SI.No. Title Authors


mushrooms on coir pith


M.N. Shashirekha


mushrooms-Urban model


M.N. Shashirekha


mushrooms-Rural model


M.N. Shashirekha

4. Process for oyster mushroom

dehydration.


M.N. Shashirekha

5. Oyster mushroom-Pickle & sweet

chutney.


M.N. Shashirekha

6. Mushroom canning S. Rajarathnam, Zakia Bano

M.N. Shashirekha


mushrooms on coffee pulp


M.N. Shashirekha.

8. Mushroom production by using

substrate supplemented with cotton

seed meal

S. Rajarathnam,Zakia Bano

M.N. Shashirekha

9. Shelf stable custard apple pulp M.N. Shashirekha

S. Rajarathnam

M.R. Vijayalakshmi

Revathy Baskaran and Ramesh M.N.

10. RTS beverage from micro-filtered

custard apple juice

Iboyiama singh,

M.R. Vijayalakshmi

M.N. Ramesh

Revathy Baskaran

M.N. Shashirekha

S. Rajarathnam

11. Instant mushroom soup mix S. Rajarathnam

Zakia Bano

M.N. Shashirekha and Ramesh, M.N.

12. Custard apple Jam, Jelly and fruit

mix

M.R. Vijayalakshmi

M.N. Shashirekha

Revathy Baskaran

M.N. Ramesh

S. Rajarathnam

13. Debittering of kinnow orange juice

for preparation of concentrate and

sweetened juice

S.K. Berry, Seghal, Shashirekha, M.N.,

Revathy Bhaskaran, K.V.R. Ramana,

Rajarathnam, S.,Venkatesh Murthy,

Mohan Lal, Vijayalakshmi, M.R, Asha

59

15. Process for preparation of product from Pear-dehydrated

M.N. Shashirekha, M.R. Vijayalakshmi, Revathy Bhaskaran, M.R. Asha, P.C.S. Nambiar and S. Rajarathnam

16. Process for preparation of product from Pear-Juice

M.R. Vijayalakshmi, M.N., Shashirekha, Revathy Bhaskaran, M.R. Asha, P.C.S. Nambiar and S. Rajarathnam

17. Process for preparation of product from Pear-Powder

Revathy Bhaskaran, M.R. Vijayalakshmi, M.N. Shashirekha, M.R. Asha, P.C.S. Nambiar and S. Rajarathnam

18. A process for herbal ripening of banana

M.N. Shashirekha,L.Jagan Mohan Rao,

M.N.Keshav Prakash, B.S.Sridhar and S.

Rajarathnam

19. A process for development of cereal flakes and spray dried powder from custard apple.

M.R. Vijayalakshmi, Revathy Baskaran

, M.N. Shashirekha, Umesh Hebbar and

S. Rajarathnam

20. A process for development of instant broccoli soup mix.

M.N. Shashirekha, M.R.

Vijayalakshmi, Revathy Baskaran and

S. Rajarathnam

21. Process for the extension of storage life of custard apple

M.N. Shashirekha, Umesh Hebbar and S. Rajarathnam.

22.

Process know-how for the preparation of blended beverages from banana

M.R.Vijayalakshmi,Shashirekha,M.N. P.C. S. Nambiar and S. Rajarathnam

23. A process for preparation of beverage and jelly from sweet

orange (Citrus sinensis) fruit

M.N.Shashirekha ,Shylaja M.Dharmesh ,S.Rajarathnam, Umesh Hebbar, Prem Vishwanath,Maya Prakash,Vijayalakshmi

24. A process for preparation of nutraceutical beverage and jelly from mangosteen rind

M.N.Shashirekha ,Shylaja M.Dharmesh ,S.Rajarathnam, Umesh Hebbar, Maya Prakash

b) List of products developed:

1. Instant natural fruit milk shake powders

2. RTS custard apple beverage

3. Stable ,frozen custard apple pulp.

4 Kinnow orange jelly.

5. Kinnow orange spray dried powder.

6. Instant mushroom soup mix.

7. Texturized banana (at lab scale)

8. Texturized mango (at lab scale)

9. Cereal flakes from kinnow orange


10. Marmalade from Kinnow orange (directly

without debittering)

11.RTS beverage from kinnow orange


12. Mushroom pickle

60

13. Mushroom sweet chutney

14. Pear juice

15. Dehydrated broccoli

16. Broccoli soup mix

17. RTS banana pseudo stem beverage

18. Mushroom biscuits

19. Mushroom flakes

20. RTS beverage from pear

21.Button Mushroom beverage

22. Pear dehydrated

23. Mangosteen rind beverage

24.Mangosteen rind jelly

25.Sweet orange de-bittered bio-functional beverage

26.Sweet orange de-bittered jelly

27.Bio-functional mushroom jelly

-------------

61

Bio- data of Co- Principal Investigator

ResearcherID Link: http://www.researcherid.com/rid/C-7670-2009

1.Name : Jagan Mohan Rao, Lingamallu.

2.Address : Dr. L. Jagan Mohan Rao

Dr. L. Jagan Mohan Rao Senior Principal Scientist,

D.No.36, 3rd

Main, Organic Chemistry,

Mahajana Layout Plantation Products Spices & Flavour Technology

Vijaya Nagar II Stage C.F.T.R.I, MYSORE - 570 020

MYSORE - 570 017 Karnataka, India.

Phone: 0821-2512352 (O) 2302498 ®

e-mail: [email protected]

3.Date of Birth and Nationality : 20-09-1954; Indian

4.Educational Qualifications :

Degree

University Division Subjects

B.Sc Andhra I Chemistry (Main); Physics; Mathematics

M.Sc. Nagarjuna I Chemistry; Specialisation : Organic Chemistry

Ph.D. Nagarjuna Chemistry*

* Title of Ph.D.,Thesis: Chemical Constituents of Anisomeles ovata R.Br.

5. Experience

Position From To Nature of work

J.R.F.(CSIR) 24-10-1977 30-04-1981 Research and Development

Res. Associate (UGC) 15-12-1983 14-12-1988 Research and Teaching

Pool officer (CSIR) 15-12-1988 01-02-1991 Research and Teaching

Scientist – B (CFTRI) 04-02-1991 03-02-1995 Research (Basic and Applied), Development of

analytical methods, Process and product

development, Technology transfer, Consultancy,

Teaching, and Guidance (Post graduate and

Doctoral studies)

Scientist – C 04-02-1995 03-02-2000

Scientist – E1 04-02-2000 03-02-2004

Scientist – E2 04-02-2004 03-02-2009

Senior Principal

Scientist

04-02-2009 To date

UNU-KIRIN Fellow April 2000 March 2001 Research

6.Research experience : 34 YEARS (for details see Annexure - I)

7. Fields of Research : Natural Products, Bio-active molecules, Food Chemistry, Synthetic

Chemistry, Flavours, Food Additives,


62

Spices and Plantation Products.

8. No. of Research Publications : 107 ( Journals of International repute such as

Phytochemistry, Heterocycles, J. Nat.Products, Carbohydrate Research, J.Chromat. Sci.,

J.Agric.Food Chem. J.Food quality, Naharung, Zeitschrift für Naturforschung C, Flav. Fragr. J.

Food Chemistry, Bioorganic and Medicinal Chemistry, European Food Research and

Technology, Journal of Food Science, Magnetic Resonance in Chemistry, CRC Critical

reviews in Food science and Nutrition, Trends in Food Science and Technology, Sciences des

aliments, Food Research International, LWT, Food and Chemical Toxicology and Indian J.

Chem) (See Annexure II)

9. Invited Lectures & Conferences contibuted : (See Annexure III)

10. Professional achievements :

a . Patents : 36 (Indian)+8 (International) (See Annexure IV)

b. Processes : 14 (See Annexure IV)

11. Guidance and Teaching :

Ph.D - Guidance

1. Chemical Investigations on selected Asclepiadaceae species having food applications.

(Degree awarded to S.Nagarajan).

2. Chemical and bioactive studies on by-products from spice processing (Degree awarded

to G.K.Jayaprakash).

3. Bio-chemical and Technological investigations on Tea (Degree awarded to B.B.Borse).

4. Studies on utilization of low-grade coffee (Thesis submitted – K.Rama Lakshmi).

5. Systematic chemical and bioactive studies on ginger (under progress – I. Rahath Kubra)

6. Studies on interaction of chemical constituents of turmeric during agglomeration (under

progress – K.Dhanalakshmi)

7. Chemical characterisation and processing of Spices for bioactivities and food

applications (under progress – Vasundhara Sharma)

Post Graduation - Guidance

Dissertations

1. Development of Green note in Black Tea. (1997-98) - M.K.Joshi

2. Natural colourants as food additives. (1998-99) - A.R.Sripriya.

3. Microwave processing of spices. (2001-02) - Sushmita Behera

4. Diversified products of the tea and recent trends (2002-03) – G. Bhanu Prakash

5. Coffee-an overview on bioactivity studies. (2003-04) - Sunitha E.George

6. An overview of coffee carbohydrates. (2004-05) – Meenakshi Arya

7. An overview on biological activities of black tea. (2005-06) - Vasundhara Sharma

8. Recent Trends in soft beverages (2006-07) – Deepika Sharma

9. Recent Trends in High impact value addition in tea (2007-08) – Maruti Prasad Panda

10. Recent trends in value addition in coffee (2008-09)- Debabrata Patra

11. Chlorogenic acids-occurrence, chemistry, technology and biological activities (2009-10) –

Rohith Upadhyay

12. Microwave processing of spices and plantation crops (2010-11)- Devender Kumar

63

Investigations

1. Determination of C6 flavourants in Black Tea. (1997-98) - M.K.Joshi

2. Enrichment of colour value in Paprika Oleresin. (1998-99) ) - A.R.Sripriya.

3. Cumin seeds: Roasting methods and attended effects on flavour quality (2001-02) -

Sushmita Behera

4. Flavoured tea: preparation, analyses and storage studies (2002-03) – G. Bhanu Prakash

5. Low grade coffee: investigative studies on its utilization (2003-04) - Sunitha E.George

6. Efficacy of Green Coffee Conserve against Oxidative Rancidity of Sunflower Oil (2004-

05) – Meenakshi Arya.

7. Effect of milk and sugar addition on antioxidant activity of black tea (2005-06) -

Vasundhara Sharma.

8. Novel functional dry beverage mixes using ginger and mint (2006-07) – Deepika Sharma

9. Chemical composition and characteristics of volatile compounds from Citrullus colocynthis

Schard –2007 – S.Gurudeeban

10. Extraction of bioactive conserves from curry leaves –2008- K.Vamsi Krishna

11. Antioxidant enriched mixed fruit jam (2007-08) – Maruti Prasad Panda

12. Effect of unconventional processing on the chemical composition of volatiles from cumin

seeds – 2008- M.V.R.Anirudh

13. Bioactive conserve from unconventionally processed cumin seeds –(2008-09)-Aarthy, M.

14. Studies on isolation of conserves from processed cumin seeds and evaluation of antioxidant

potential–2009-Bhavyasree

15. Effect of enzymes treatments on coffee (2008-09)- Debabrata Patra

16. Separation and characterisation of low polar anti-oxidant molecules from Ginger (2009)-

Mhatre Jayesh Dinesh

17. Fractionation of conserves from roasted cumin seeds and their antioxidant potential (2010)

- Poornima Gowthaman

18. Microwave assisted extraction of chlorogenic acids from the green coffee beans (2009-10) -

Rohith Upadhyay.

19. Chemical composition of essential oils from processed cumin seeds using alternate energy

in pilot scale-V.Vijaykrishna and M. Rekha

Teaching

1. Member of the M.Sc (Chem) faculty at Nagarjuna University from 1978 to 1991.

2. Member of the M.Sc.(Food Tech.) faculty at C.F.T.R.I. for the last 18 years.

12. Awards and Honours : (see Annexure –V)

13. Number of Projects involved : 37 (see Annexure –VI)

14. Review of research articles / manuscripts: Flavour Fragrance Journal; Journal of

Agricultural and Food Chemisty; Carbohydrate Research; Journal of Food Composition

and Analysis; International Journal of Food Science and Technology; European Food

Research and Technology; Journal of Food Biochemistry; Food Chemistry; Journal of

Chromatographic Sciences; Journal of Undergraduate Chemistry Research; Indian

Journal of Chemistry and Journal of Food Science and Technology.

64

15. Contributions to Professional organisations:

Life member of Association Food Scientists and Technologists (I), Society of

Biological chemists, Institution of Chemists and Indian council of Chemists

Society of Biological chemists

Elected and served as ‘Secretary’ of SBC, Mysore Chapter during 2005-06

Association Food Scientists and Technologists (I),

Actively worked in Scientific / Technical committees for the last several

years in National and International symposia organised by AFST(I).

ICFOST 2005 – Co-chairman, Poster Committee

ICFOST 2007 – Convenor, Technical Committee

IFCON 2008 - Convenor, Poster Committee

ICFOST 2009 – Chairman, Poster Committee

ICFOST 2011 – Chairman, Poster Committee

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Bio- data of Co- Principal Investigator

A. Name : Dr. Shylaja M. Dharmesh

B. Date of Birth : 01-06-1957

C. Institution : Department of Biochemistry and Nutrition

E. Academic Qualification and professional career :

Degree/position Year Place Subject

________________________________________________________________________

Principal 2007 -Present CFTRI, Mysore Molecular modeling &

Scientist (EII) Signal transduction pathways

Senior 2003-2007 CFTRI, Mysore Cancer/ ulcer biology

scientist (EI) Nutraceticals

Scientist (C) 1999-2003 CFTRI,Mysore Cell Biology/

Antioxidants/

Senior RA 1993-1999 Dept. Biotechnology, Immunoassays

Mysore Teaching

Life sciences

RA 1991-1993 Dept. Pathology, WASHU Molecular

St.Louis, USA Endocrinology

PDF 1989-1991 Dept. Biol.Chem. Tumor cell

HMS, Boston Differentiation

USA /ECM

STA 1987-1989 CFTRI, Mysore Dietary

Antioxidants

Lecturer 1986-1987 Dept. Biochemistry Teaching

MGM, Mysore Reproductive

Biology

Research 1980-1986 Dept. Biochemistry Teaching

fellow MGM, Mysore Glycoproteins

M.Sc 1977-1979 Dept. Biochemistry Biochemistry

MGM, Mysore

66

F. Awards/Recognition/Prizes/Certificates:

• Received Best Technology Transfer Award –CFTRI Annual Awards 2011

Received First Prize for one of the posters presented at the International Conference on

Cancer and Holistic medicine, 2011

• Received an Appreciation Certificate from African Journal of Biotechnology, 2011

Selected for CFTRI Best Paper Award, 2010

Nominated and selected for Marquis Who’s who in Science and Engineering, 2010

Selected as a Member of National Academy of Sciences, NASI, Allahabad, INDIA, 2009

Nominated and selected for Marquis Who’s who in Biomedicine and healthcare, 2009

Nominated and received a silver medal as one of the 2000 outstanding scientists in the

world by International Biographic Centre, Cambridge, U.K. 2009

Awarded with Best Student Award during 2009 for one of the Ph.D students

Awarded with Best Scientist CFTRI award during 2008;

Nominated and selected for Marquis who’s who in the world, 2008.

Selected for following awards for R & D of the lab announced during national and

international symposiums: Best poster award 2009, Best Paper travel award, 2009; Best

paper award in 2008; Best poster award in 2005 and Merit award in 2004.

Nominated as an editorial board member for the Journal of Alternative Medicine in 2007.

Selected as Board of Examiners and Board of Studies in Biochemistry, Biotechnology and

Food Science and Technology and Molecular Biology, 2005 - till present.

Awarded with DBT / DST / CSIR and DAE projects during 2001 – till present .

Recognition and appreciation certificate: received for good efforts in research - Harvard

Medical School, Boston, USA, 1991.

Best Paper Award for the year 1993, Russell Scientific,France;

Honored by being invited for delivering lectures at national and international symposiums

G. Publications: Book Chapter: 03

Research Papers and reports: 56

General Articles: 04

Patents/Process: 11/4

Other [Technical reports]: 04

Reviews/presentations: 01/more than 75

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Details of publication – Please see later

Fellowship/Membership of Professional Bodies:

Membership: Selected as a Member of NASI, Allahabad, INDIA, 2009

:

1. Society of Biological Chemists (India) – Life member

2. Indian Association of Nuclear Chemists

And allied Scientists – Life Member

3. Indian association of Cancer Research - Member

4. Indian Science Congress - Ordinary Member

5. Association of Microbiologists of India – Life Member.

6. Society of Carbohydrate chemists, India – Member

7. Association of Food Scientists & Technologists (India) – Member

8. Association of Carbohydrate Chemists and Technologists – Life member

Fellowships:

1.Awarded with a CSIR – SRF during April 2007, & April 2005 ; an ICMR – SRF in

July 2006, for the Ph.D. projects proposed.

2.Awarded with CSIR Research Associateship during 1994 till 1999.

3.Awarded with Fellowship (Post-doctoral) from Washington University School of

Medicine, St. Louis, USA during 1991 – 1993.

4.Awarded with Fellowship (Post-doctoral) from Harvard University at Harvard

Medical School, Boston, Massachusetts, USA, during 1989 – 1991.

5.Awarded with UGC Senior Research Fellowship from New Delhi during 1984 – 86.

6.Awarded with UGC Junior Research Fellowship from New Delhi during 1980 – 83.

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Details of Publications:

1. Srikanta, B.M., Harish Nayaka, M.A & Dharmesh, S.M. (2011): Inhibition of Helicobacterpylori growth and its cytotoxicity by 2-hydroxy 4-methoxy benzaldehyde of Decalepis hamiltonii (wight & Arn); a new functional attribute. Biochimie, 93, 678-688 [I.F. 3.87]

2. Mahendra C.K., Sathisha, U.V, Dharmesh, S.M, Appu Rao, A.G & Sridevi, A.S. (2011): Interaction of sesamol (3,4-methylenedioxyphenol) with tyrosinase and its effect on melanin synthesis. Biochimie, 93, 562-569. [I.F. 3.87]

3. Smitha, J., and Dharmesh, S.M. (2011): Assessment of antioxidant potentials of free and bound phenolics of Hemidesmus indicus (L) R.Br against oxidative damage, Pharmacognosy Res. 3, 225-231

4. Smitha, J., and Dharmesh, S.M. (2011): H+K+ATPase/ H.pylori inhibitory and antioxidative components from dietary sources’; International Journal of Food and Nutrition [I.F 1.223 ]

5. Renu Agrawal and Dharmesh,S.M. (2011): An anti-Shigella dysenteriae

bacteriocin fromPediococcus pentosaceus MTCC 5151 cheese isolate, Turk J Biol. 35, 1-9 [I.F. .857]

6. Smiha, J., and Dharmesh, S.M.(2011): Antiproliferative Effect of Antioxidative Free and Bound Phenolics from Andrographis Serpyllifolia, Free Rad.Antioxidants, 1, 17-25.

7. Lakshminarayana, R,, Aruna, G,, Sathisha, U.V., Dharmesh,S.M. and Bhaskaran, V. (2010): Antioxidant and cytotoxic effect of oxidized lutein in human cervical carcinoma cells (HeLa). Food Chemical Toxicology, 48, 1811-1816. [I.F. 2.721]

8. Vismaya, Srikanta B.M., Sindhu R., Vinay B.J, Dharmesh S.M. and Sindhu Kanya TC (2010): Gastroprotective properties of karanjin from karanja (Pongamia pinnata) seeds; role as antioxidant and H+, K+- ATPase inhibitor. Evidence Based Complementary and Alternative Medicine. DOI:10.1093/ecam/neq 027. [I.F. 2.964]

9. Chandrappa, S., Srikantaka, B.M., Dharmesh, S.M. and Rangappa, S. (2010): Inhibition of Gastric H+, K+-ATPase by novel thiazolidinone derivatives. Journal of Sulfur Chemistry,31, 189-196. [I.F. .765]

10.Srikanta B.M., Sathisha, U.V. and Dharmesh S.M. ((2010): Alterations of matrix metalloproteinases, gastric mucin and prostaglandin E2 levels by pectic polysaccharide of swallow root (Decalepis hamiltonii) during ulcer healing. Biochimie, 92 194 – 203. [I.F. 3.787]

69

11. Harish Nayaka, M.A., Sathisha, U.V., and Dharmesh S.M. ((2010): Cytoprotective and antioxidant activity of free, conjugated and insoluble-bound phenolic acids from swallow root (Decalepis hamiltonii). Food. Chemistry, 119, 1307 - 1312. [I.F. 3.922]

12. Balasubramanian, K., Vasudevamurthy R, Venkateshaiah SU, Thomas A, Vishweshwara A, Dharmesh, S.M. (2009): Galectin-3 in urine of cancer patients: stage and tissue specificity. J Cancer Res Clin Oncol 2009 135:355–36 [I.F 2.3]

13. Harish Nayaka, M.A., Sathisha, U.V., Manohara, M.P., Chandrashekara, K.B. and Dharmesh, S.M. (2008): Cytoprotective and antioxidant activity studies of jaggery sugar. Food Chemistry, 2008, doi:10.1016/j.foodchem.2008.11.067. Article in Press.[I.F. 3.09]

14. Madhusudhana KN, Srikanta BM, Dharmesh S.M., Prakash H.S. and Shetty H.S. (2009): Changes in antioxidant enzymes, hydrogen peroxide, salicylic acid and oxidative stress in compatible and incompatible host-tobamovirus interaction. Journal of Plant Interactions. 4 (3), 157-166.

15. Nagesh, K. S., Harish Nayaka M. A., Dharmesh, S.M., Shanthamma, C. and Pullaiah T. (2008). In vitro propagation and antioxidant activity of Curculigo orchioides. Tropical Journal of Medicinal Plants, 9-13.

16. M.N. Rekha, A. Ramesh Yadav, Dharmesh, S.M, A.S. Chauhan, Ramteke, S. (2008): Evaluation of antioxidant properties of soup mix extracts containing Dill (Anethum sowa L) leaf; Food and Bioprocess Technology, In Press.[I.F. ]

17. Chethan, S., Dharmesh, S.M. and Malleshi N.G .(2008): Inhibition of aldose reductase from cataracted eye lenses by finger millet (Eleusine coracana) polyphenols. Bioorg Med Chem,16,10085-90. [I.F. 2.68]

18. Kamath, B.S., Srikanta, B.M., Dharmesh, S.M., Sarada, R. and Ravishankar G.A. (2008): Ulcer preventive and antioxidative properties of astaxanthin from Haematococcus pluvialis. Eur J Pharmacol. 590:387-95. [I.F. 2.9]

19. Mahendra ,C.Kumar, Sathisha, U.V., Dharmesh, S.M, Appu Rao, A.G and Sridevi Annapurna Singh (2008): Effect of sesamol, a potent tyrosinase inhibitor, on melanin and melanin synthesizing melanoma cells. As, Proceedings of The FASEBJournal.2008;22:1062. [I.F.6.72]

20. Harish Nayaka, M. A., Sathisha, U. V. , Chandrashekar, K. B., Manohar, M. P. and Dharmesh, S.M. (2008). Evaluation of antioxidant activity of bound phenolics of sugarcane under in vitro conditions Sugar Tech (2008) 10: 302-307. [I.F. 3.87]

21. Rajesh, J, Harish Nayaka, M.A, Basavaraj, M., Dharmesh, S.M., Karuna Kumar M., Ravishankar, G.A. (2008): Antioxidant potential of secoisolariciresinol

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diglucoside isolated from defferent fractions of Flaxseeds. Seed science and Biotechnology, 2, 83-88. [I.F. 3.39]

22. Srikanta, B.M., and Dharmesh, S.M. (2007): Is there any alternative to control multisteps of ulcers?. Published in EurekAlert, http://www.eurekalert.org/pubnews.php, Release date: 23-Oct-2007. [News]

23. Srikanta, B.M., Siddaraju, M.N. and Dharmesh, S.M. (2007): A novel phenol-bound pectic polysaccharide from Decalepis hamiltonii with multi-step ulcer preventive activity. World J. Gastroenterol. 13, 5196-5207. [I.F. 3.34]

24. Sathisha, U.V., Smitha, J., Harish Nayaka, M.A., Dharmesh, S.M. (2007): Inhibition of Galectin – 3 mediated cellular interactions by pectic polysaccharides from dietary sources. Glycoconjugate J., 24, 497 – 507. [I.F. 7.4]

25. Siddaraju, M.N. and Dharmesh,S.M. (2007):Inhibition of gastric H+,K+-ATPase and Helicobacter pylori growth by phenolic antioxidants of Curcuma amada. J. Agric. Food Chem. 55,7377-7386. [I.F. 2.6]

26. Yogender Naik, Smitha, J., Harish Nayaka, M.A., Lakshman, and Dharmesh, S.M (2007): Gastroprotective effect of swallow root (Decalepis hamiltonii): possible involvement of H+- K+- ATPase inhibition and antioxidative mechanism. J. Ethnopharmocol.. 112, 173-179. [I.F. 1.55]

27. Siddaraju, M.N., and Dharmesh, S.M. (2007): Inhibition of gastric H+-K+-ATPase and H. Pylori growth by phenolic antioxidants of Zingiber officinale. Mol. Nutrition & Food Res. 51, 324 – 332. [I.F. 3.2]

28. Harish Prashanth K.V., Dharmesh, S.M., Jagannath Rao K.S. and Tharanathan R.N. (2007): Free radical-induced chitosan depolymerized products protect calf thymu DNA from oxidative damage. Carbohydrate Res. 342, 190-195. [I.F. 1.5]

29. G Suresh Kumar, M.A Harish Nayaka, Dharmesh, S.M. and PV Salimath (2006): Free and Bound Phenolic Antioxidants in Amla (Emblica officinalis) and Turmeric (Curcuma longa). Journal of Food Composition and Analysis. 196, 446-452. [I.F. 1.1]

30. Nethravathi, G.P., Sathish, U.V., and Dharmesh, S.M., Shashirekha, M.N. and Rajarathnam, S. (2006): Antioxidant activity of Indigenous Edible Mushrooms, J.Agric.Food.Chem. 54, 9764 – 9772. [I.F. 2.6]

31. Nalini, M.S., Dharmesh, S.M., Prakash, H.S. and Shetty, H.S. (2006): Production of polyclonal antibody to bean common mosaic virus and its application in seed health testing, Ind. J. Microbiol. 46, 97 – 108. [ I.F ]

http://www.eurekalert.org/pubnews.php

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32. Begum, R., Lokesh, S., Dharmesh, S.M., Rai, R. and Shetty, H.S. (2005): Evaluation of certain storage conditions for Okra (Abelmoschus esculentus (L.) Monech) seeds against potential fungal pathogens. Int. J. Agri and Biol. 7, 550-554. [I.F ]

33. Nalini, M.S., Prakash, H.S., Dharmesh, S.M., and Shetty, H.S. (2004): Indexing of French bean (Phaseolus Vulgaris L.) for bean common mosaic virus infection. Seed Research 32, 203 – 209. [I.F. ]

34. Puttaraju, H.R., Prakash, H.S., Dharmesh, S.M., and Shetty, H.S. (2004): Purification of Blackeye cowpea mosaic potyviurs: Production of polyclonal antibodies and its application in Seed health testing. J. Mycology and Plant Pathol. [I.F. ]

35. Rajeshwari, N., Dharmesh, S.M., Krishnappa., Shetty, H.S., Mortensen, C.N and Marthur S.B.(2003): Seed Science and technology, 18, 559-565.(Proceedings)

36. Rajeshwari, N. Dharmesh, S.M., Krishnappa, M., Shetty, H.S., Mortensen, C.N. and Marthur, S.B. (2002): Incidence of Ralstonia solanaceaum induced wilt in Tomato; detection of pathovars. Ann. Rev. Phytopathology, 24, 1-16. (Proceedings)

37. Madhu, D., Dharmesh, S.M., Chandrashekar, A., Shetty, H.S., and Prakash, H.S. (2001): Role of H+ ATPase in pearl millet downy mildew disease resistance, Plant Science, 161, 799-806. [I.F. 2.5 ]

38. Umesha, S., Dharmesh, S.M. and Shetty, H.S. ( 2000 ) : Lytic factors : their involvement in downy mildew disease resistance of pearl millet. Plant Science, 157, 33-41. [I.F. 2.5]

39. Vijayanand, G.K., Dharmesh, S.M., Krishnappa, M., and Shetty, H.S. [1999] : A trategy to generate polyclonal antibodies to Xanthomonas campestris.pv.cyamopsidis; its application in indexing of guar seeds, J.Appl.Microbiol, 87, 711-717 [I.F. 3.1 ]

40. Rajeshwari, N., Dharmesh, S.M., Krishnappa, M and Shetty H.S [1999] : A Note for the field evaluation of bacteril wilt induced by Ralstonia Solanacearum in Tomato, Bact.Wilt.News Lett.

41. Dharmesh, S.M., Krishnappa, M., Rajeshwari, N., Shetty, H.S., Mortensen, C.N. and Mathur, S.B [1999] : Stategies and problems in the development of Immunoassays for phytobacterial pathogens to employ in seed health testing , In proc. Seed Science and Technology, eds. Shekar shetty, H and Prakash, H.S; pp. 200-206.

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42. Rajeshwari, N., Dharmesh, S.M., Krishnappa, Shetty, H.S., Mortensen, C,N.and Mathur, S.B [1998] : Develooment of ELISA for the detection of Ralstonia solanacearum in Tomato, its use in seed health testing. World.J.Microbiol.& Biotechnol [ I.F. 3.1 ]

43. Rajeshwari N., Dharmesh, S.M., Krishnappa, M., Shetty, H.S., Mortensen, C.N. and Mathur, S.B. (1998) : Bacterial wilt induced by Ralstonia solanacearum; diagnosis by ELISA. Bact. Wilt News Lett.

44. Manzella, S.M., Dharmesh S.M., Cohick, C.B., Soares, M.J. and Baenziger, J.U. (1997): Developmental regulation of a pregnancy-specific oligosaccharide structure, NeuAca2,6GalNAcb1,4GlcNAc, on select members of the rat placental prolactin family. J. Biol. Chem. 272, 4775-4782. [I.F. 7.2 ]

45. Umesha, S., Dharmesh, S.M., Shetty, S.A., Krishnappa, M., and Shetty, H.S.[1996]: Biocontrol of downy mildew disease of pearl millet using Pseudomonas fluorescence. Crop protection (UK), 17, 387-392. [ I.F. 1.1 ]

46. Umesha, S., Dharmesh, S.M., and Shetty, H.S. (1995): Molecular mechanism of downy mildew disease resistance in pearl millet. Indian J. Mycol. Pl. Pathol., 25, 59p.

47. Manzella, S.M., Dharmesh, S.M., Beranek, M.C., Swanson, P., and Baenziger, J.U. (1995): Evolutionary conservation of the sulfated oligasaccharides on vertebrate glycoprotein hormones that control circulatory half life. J. Biol. Chem. 270,21665-21671. [ I.F. 7.2 ]

48. Dharmesh, S.M. and Baenziger, J.U. (1993): Estrogen modulates expression of the glycosyltransferase that synthesize sulfated oligosaccharides on lutropin. Proc. Natl. Acad. Sci. USA. 90, 11127-11131. [ I.F. 11.2 ]

49. Dharmesh, S.M., Skelton, T.P. and Baenziger, J.U. (1993): Co-ordinate and Restricted expression of the ProXaa Arg/Lys-specific GalNAC-transferase and the GalNAC b1,4, GlcNAC b1,2 Man a-4 sulfotransferase. J. Biol. Chem. 268, 17096-17102. [ I.F. 7.2 ]

50. Smith, P.L., Skelton, T.P., Fiete, D., Dharmesh, S.M., Beranek, M.C., Macphael, L., Broze, G.J., and Baenzinger, J.U. (1992) : The Asparagine Linked Oligosaccharides on tissue factors pathway inhibitor Terminates with SO4-4 galNAC b1,4 GlcNAC b1,2 man a. [I.F. 7.2 ]

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51. Srinivas, L., Shalini, V.K., and Dharmesh, S.M. (1992): Turmerin, a water Soluble antioxidant peptide from turmeric Curcuma Longa. Arch. Biochem. Biophys. 292, 617-623. [I.F. 3.1 ]

52. .Shylaja M. (1988): Mysteries of cells. In the Proc. of Natl. Workshop, CFTRI, Mysore.

53. Shylaja M. and Sheshadri, H.S. (1989): Glycoprotein an overview, Biochem. Edn. 17, 179-188.

54. Shylaja M., and Sheshadri, H.S. (1986): Metabolism of toad Bufo melanostictus Egg Jelly Glycoprotein. Proc. Ind. Acad. Sci.

55. Shylaja M. and Sheshadri, H.S. (1985): Studies on some enzymes of toad Bufo melanostictus testes and their probable role at the tissue of fertilization. Experientia. 41, 1113-1118.

Technology Transferred/Patents, if any. (State whether patents were commercialized).

Process:

1. Rajarathnam, S., Shashirekha, M.N. and Dharmesh, S.M (2010): A process for the preparation of nutraceutical jelly from mangosteen rind.

2. Rajarathnam, S., Shashirekha, M.N. and Dharmesh, S.M (2010): A process for the preparation of biofunctional beverage from sweet orange (Citrus sinensis) – commercialized, Exclusive – 10 Lakhs for three years

3. Rajarathnam, S., Shashirekha, M.N. and Dharmesh, S.M (2010):A process for the preparation of biofunctional beverage from mangosteen rind.

4. Rajarathnam, S., Shashirekha, M.N. and Dharmesh, S.M (2010): A process for the nutracetucal jelly from sweet orange .

Patents:

1. Rajarathnam, S., Shashirekha, M.N. and Dharmesh, S.M (2011): A process for the preparation of biofunctional products from Apple pomace, submitted

2. Rajarathnam, S., Shashirekha, M.N. and Dharmesh, S.M (2010): A process for the preparation of Nutraceutical jelly from Mangosteen rind, submitted

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3. Rajarathnam, S., Shashirekha, M.N. and Dharmesh, S.M (2010): A process for the preparation of Bio-functional beverge from sweet orange (Citrus sinensis) submitted

4. Renu Agarwal and Dharmesh, S.M (2009): A process for the preparation of anti-shigella bioactive fraction from Prebiotic lactic acid bacteria – No. 432/DEL/2010

5. Smitha, J., and Dharmesh, S.M. (2008): A process for the preparation of antimetastatic polysaccharide from Turmeric (Curcuma Longa) – No. 314/DEL/2009

6. Smitha, J., Sathisha, U.V., Srikanta, B.M. and Dharmesh, S.M. (2007): An anti-invasive and antimetastatic galectin-3 inhibitory polysaccharide from Decalepis hamiltonii and a process for the preparation thereof. – No. 0808/DEL/2008

7. Siddaraju, M.N., Srikanta, B.M. and Dharmesh, S.M. (2007): A process for the preparation of antiulcer powder from Swallow root (Decalepis hamiltonii)- No. DEL – 705/DEL/2007

8. Harish Nayaka, M.A., and Dharmesh, S.M. (2005): A process for the preparation of Galectin inhibitory antimetastatic polysaccharide from Swallow root (Decalepis hamiltonii) – No. 305/DEL/2006;

9. Siddaraju, M.N., and Dharmesh, S.M. (2006): A bioactive fraction from zingiber officinale and a process for the preparation thereof – No. 900/DEL/2006; Recommended for International Patent.

10. Harish Nayaka, M.A., and Dharmesh, S.M. (2004): A process for the development of bioactive compounds from Swallow Root (Decalepis hamiltonii) submitted.

11. Harish Nayaka, M.A., Lokesh, B.R., and Dharmesh, S.M. (2003): A process for

the development of antioxidant powder from Black Cumin (Nigella sativa). Patent

No. 243225

Popular Articles:

1. Umesh S., Dharmesh S.M., and Shetty, H.S., (1997) : Downy mildew disease in pearl millet. The Hindu, December 8, 1997.

2. Shetty, H.S., Dharmesh, S.M and Shetty, H.S (1998): Pearl millet and

Mildew disease., In Indian express., Science express, June 23, 1998.

3. Dharmesh, S.M, (1993): Orchids beauty; In Kannada Prabha, Local News paper, Mysore.

4. Dharmesh, S.M, (1994): Humanly attitude in the jail; Alcatraz, Star of Mysore, Mysore.

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Articles published in newsletter / Bulletin:

1.Dharmesh,S.M., Cancer Immunology (2007) : Newsletter of Soc. of Louis Pasteur, University of Mysore, Mysore. 2.Dharmesh, S.M., Molecular Techniques in the detection of plant pathogens,

(1998): Tech. Bulletin of Dept. of Appl.Botany and Biotechnol, University of Mysore, Mysore.

3.Dharmesh, S.M., Host-pathogen interaction, (1997): Newletter of Soc. Appl.Botany and Biotechnol, University of Mysore, Mysore

Reports:

1. Dharmesh, S.M., Prakash, H.S and Shetty, H.S. (1998): Molecular

interaction in plant pathology, Dept. of Appl.Botany and Biotechnol, UOM, Mysore.

2. Dharmesh, S.M., Prakash, H.S and Shetty, H.S. (1999): Development of

sensitive, specific and reliable imunoassays for the detection of Plant pathogens,

Dept. of Appl.Botany and Biotechnol, UOM, Mysore.

3. Dharmesh, S.M.. (2004): Dietary carcinogens and anticarcinogens, CFTRI,

Mysore.

4. Dharmesh, S.M.. (2006): Anticancer attributes of food, CFTRI, Mysore.

5. Dharmesh, S.M.. (2006): Phytomedicines, for international symposiums on

applied Biotechnology, Mysore.

6. Dharmesh, S.M.. (2007): Animal models in cancer research, CFTRI, Mysore.

7. Dharmesh, S.M.. (2008): Animal models in cancer research, CFTRI, Mysore.

8.Dharmesh, S.M.. (2010): Animal models /anticarcinogencity in cancer research, CFTRI, Mysore.

9. Dharmesh, S.M.. (2011): Role of diet in Animal research.

10. Dharmesh, S.M.. (2011): HPLC analysis for estimation of all fat soluble vitamins

Other types of publications, if any (like CDs etc.):

Prepared many reports including calibration of the instrument,

standardization of methodologies, uncertainity reports since participating in several

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projects of the institute to help NABL / ISO accreditation; looking after cell culture facility

to develop cell culture models to evaluate health beneficial, disease curing nutraceuticals.

Invited Conferee and Invited lectures : From 2003 – till present.

Invited conferee: At Department of Microbiology, Shankaraghatta, 2010; At the international women science congress, 2009; At the International symposium on Cancer in 2008, 2009; National symposium on Emerging Trends in Microbiology during 2006 in Department of Microbiology; in Applied Botany during 2004 , Seed Science and Technology, 2004, India; At the national symposium on snake venom toxins and trends in Biochemistry, 2004, India; at the national symposium on snake venom and toxins held during January 2004. Invited Speaker: Delivered a lecture at the International Symposium on Cancer, 2008; Frontiers in Phytomedicines during Inaugural function of Botanical Society of University of Mysore in 2008; on Animal Models in Cancer Research in the short-term training programme held during 14-18 May 2007; on Cancer and Microbes and Molecular Medicines at Kuvempu University during 3 - 4 May 2007; on Cancer Immunology during March 10, 2007; on Phytomedicines in August 2006 at Kuvempu University; on Anticancer Attributes of Food in an International work shop held during April 3rd to 7th 2006; At CFTRI, during an International Workshop held during April 7th 2006 on Anticancer attributes of food; to Deliver a Lecture on Cancer; challenges in cancer therapeutics on September 2005; Current Trends in Alternative Medicines with particular emphasis to cancer at Gordan College; Molecular medicine in cancer, Department of Biotechnology during November 2005, At Amrithanandamayi college, University of Mysore on National Science Day on Microbes and Cancer March 2005, At Kuvempu University on National Science Day, Gene cloning and its applications, Davanagere 2004; Genomics and Proteomics - as a panel member, 2004, at Ayurveda Medical College - Emerging trends in Ayurveda Drugs for Cancer, 2003; At the University of Mysore, Mysore - Orientation Programme to teachers on Chemistry of anticarcinogens, 2003; to deliver a National Science Day seminar, at the Department of Microbiology, Kuvempu University, Davangere, February 2004; to deliver technology day seminar at Vishweshwara Institute of Technology, Bangalore 2003. Served as a Ph.D. examiner, evaluated the thesis entitled 1. Studies on the Molecular Mechanism(s) of Apoptosis Induced Various DNA Damaging Chemotherapeutic Drugs in High _Risk HPV E-6 Positive Carcinoma” of Pune Uniersity in February 2007; Mechanism of anti-diabetic principle from Ayurvedic herbs of Shivaji University 2006; “A new class of pyrimido(4,5; 4,5 thieno(2,3-b)quilolines; DNA binding and biological properties” of Kuvempu University in August 2006.

Served as Board of Examiners in Biochemistry and Food Technology at Kuvempu/Mysore University for the year 2006 to 2008; Board of studies in Biochemistry and Molecular Biology, University of Mysore, Mysore, 2007; Board of studies in Biotechnology, Molecular Biology; 2005-2008. Served as Reviewers of articles on cancer, ulcer, nutraceuticals from various national and international journals on invitation.

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Awarded with a projects submitted for CSIR – SRF during April 2007, and 2005 and ICMR – SRF in July 2006.

Projects Awarded: From 2002 onwards:

1. A project entitled “Dietary antimetastatic compounds; it’s efficacy and

bioavailability in vitro and in vivo” - DBT. Duration : 2002 – 2005. Team members :

Dr. Shylaja.M.Dharmesh - Principal Investigator

2. A project entitled “Ulcer preventive components from edible root extracts”. OLP- 0080. Duration – 2002-2003

Team members :

Dr. Shylaja.M.Dharmesh - Principal Investigator 3. A project entitled “Vitamin analysis in food samples”. ATC-1/2001. Duration - 2001-2003. Team members: Dr. Shylaja.M.Dharmesh & others - Project members

4. A project entitled “Galectin and dietary Galectin inhibitors in tumor spread /

metastasis” - DST. Duration: 2004 – 2009. Team members :

Dr. Shylaja.M.Dharmesh - Principal Investigator Dr.G.Muralikrishna - Principal Co-Investigator Dr. Raghupathi - Collaborator

5. A Project entitled “Dietary components as modulators of oxidative stress induced pathological pathological conditions. MLP - 041. Duration - 2005 - 2008

Team members

Dr. Salimath, P.V - Principal Investigator

Dr. Shylaja M. Dharmesh - Team Member

6. A project entitled “Development of a reliable, sensitive and specific radiolebelling assay to diagnose and understand the effect of dietary antioxidants on diabetic nephropathy” - GAP 216. Duration 2002-2005

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Team members: Dr. Shylaja.M.Dharmesh - Principal Co-Investigator Dr. Sambiah - Project member

Dr.Salimath.P.V - Principal Investigator

7. A project entitled “Role of probiotic Lactic acid bacteria against shigellosis” DST - Duration 2007 – 2009 Team members :

Dr. Shylaja.M.Dharmesh - Principal Co-Investigator

Dr.Renu Agarwal - Principal Investigator

8. A Project entitled “Molecular mechanisms of selected bioactive polysaccharides from swallow root (Decalepis hamiltonii) and Carrot (Daucus carrota)”. MLP – 054. Duration – 2009 – 2011.

Team members



8. A Project entitled “Mechanism, epidemiology and amelioration of degenerative metabolic disorders and the role of targeted dietary factors”. MLP – 0094. Duration – 2010 – 2012.

Team members



10. A project entitled “Design and development of equipment in appropriate and adaptable automation for hygienic and safe production of processed and semi processed foods in large scale”, NWP 02. Duration – 2007-2012.

Team members

Dr. Raghava Rao - Principal Investigator

Dr. Shylaja M. Dharmesh /others - Team Member

10. A project entitled “Development of processed fruit and vegetable products rich in Beta carotenoids and other accessory nutritional factors as food supplements”, SIP 002. Duration – 2007-2012.

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Team members

Dr. Appu Rao - Principal Investigator

Dr. Rajarathnam - Team leader

Dr. Shylaja M. Dharmesh /others - Team Member

12. Analysis of vitamins for NABL samples. Duration: 1999 - Till present

Team members: Dr. Shylaja.M.Dharmesh & others - Project members

Participated in formulation and execution of projects. Funds have been raised by writing projects to external funding agencies (DST, DBT, and CSIR) and also participated in consultancy projects on vitamin. They are as follows:

14. A project entitled “Chemsitry and food applications of Cactus (Opuntia ficus

indica)”, Project sanctioned from DBT, 2010

Team members: Dr. Shylaja.M.Dharmesh & others - Principal Co-Investigator Dr. Rajarathnam Principal Investigator 15. A project entitled “Preparation of health products from Bael Fruit (Aegle

Marmelos) (Linn.); Evaluation of antiulcer, Antidiabetic and anticancer potentials,

from TRIFED, 2012-2013

Team members:

Dr. Shylaja M Dharmesh - Principal Investigator

Dr. Salimath, P.V - Principal Co-Investigator

Dr. Rajarathnam, S - Principal Co-Investigator

Papers Presented: (2003 – till present):

Papers Presented: 1. Kavitha, B.C and Dharmesh, S.M (2012): Antiulcer potentials from Potato

(Solanum tuberosum) ; presented at ICFOST, 2012, Pune.

2. Sindhuja, H.N and Dharmesh, S.M (2011): Role of tyrosinase and galectin-Inhibitors from dietary sources against Metastasis Presented at international

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conference on cancer and holistic medicine, held during September 11-13, 2011 at Kottayam, Kerala. Received First Prize for Best Poster.

3. Meghashri, Sindhuja, H.N and Dharmesh, S.M (2011): Molecular basis of Tyrosinase inhibition during metastasis inhibition. Presented at international conference on cancer and holistic medicine, held during September 11-13, 2011 at Kottayam, Kerala.

4. Srividya, Renu Agarwal and Dharmesh, S.M (2010): Mode of anti-shigella activity by probiotic lactic acid bacteria; an in vitro study, Selected for oral presentation at the National Conference on Advanced Trends and Perspectives in Biotechnology – Implication in Agriculture and Industry, held during March 2010 at Pooja Bhagawat Memmorial Post-Graduate Centre, Mysore.

5. Balakrishna Naidu, Leela Bai and Dharmesh, S.M (2010): Potential nutraceutical properties in Fenugreek and Curry leaves; Newer bio-functional attributes; Presented as a National Conference on Advanced Trends and Perspectives in Biotechnology – Implication in Agriculture and Industry, held during March 2010 at Pooja Bhagawat Memmorial Post-Graduate Centre, Mysore.

6. Kavitha, B.C. Srikanta, B.M. and Dharmesh, S.M (2010): Antiulcer property of potato (Solanum tuberosum) pectic polysaccharide. Presented at International Conference onAdvances in Free Radicals Research “Natural Products, Antioxidants and Radioprotectors inHealth and Ningth Annual Meeting of the Society of Free Radical Research held during 11 – 13 January, 2010 at Hyderabad. Received BEST PAPER TRAVEL AWARD.

7. Sindhuja, H.N., Sathisha, U.V. and Dharmesh, S.M (2010): Potential Role of Antioxidants, Tyrosinase/Galectin-3 Inhibitor against Melanoma Cell Induced Metastasis, Presented at International Conference onAdvances in Free Radicals Research “Natural Products, Antioxidants and Radioprotectors inHealth and Ningth Annuaal Meeting of the Society of Free Radical Research held during 11 – 13 January, 2010 at Hyderabad.

8. Srikanta B.M. and Dharmesh, S.M (2009): Explicit gastroprotective role of HMBA from Decalepis hamiltonii; a new functional attribute. Presented at Indian convention of Food Scientists and Technologists, (ICFOST) – 2009. NIMHANS Convention Centre, Bangalore, December 21 - 23, 2009. Received 2nd BEST POSTER AWARD.

9. Srikanta B.M,, Sathisha U.V. and Dharmesh, S.M. (2009): Anti-H. pylori attribute of 2-hydroxy-4-methoxy benzaldehyde of swallow root - Decalepis hamiltonii. Presented at International conference on Advances in Free Radical Research and 8th Annual meeting of Society for Free Radical Research, India. CSM Medical University, Lucknow, March 19-21,

10. Srikanta B.M,, Sathisha U.V. and Dharmesh S.M. (2009): Anti-H. pylori attribute of 2-hydroxy-4-methoxy benzaldehyde of swallow root - Decalepis hamiltonii. Presented at International conference on Advances in Free Radical

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Research and 8th Annual meeting of Society for Free Radical Research, India. CSM Medical University, Lucknow, March 19-21,

10. Sathisha, U.V., and Dharmesh,S.M. (2008): Modulation of galectin-3 by a dietary galectin inhibitor - Decalepis hamiltonii presented in 2008 Annual Meeting of the Society for Glycobiology” held at Fort Worth, Texas from November 12-15, 2008.

11. Sathisha, U.V.and Dharmesh,S.M., (2008): Development of polyclonal antibody to galectin-3; its sensitivity, specificity and applicability in vitro and in vivo Presented at International Symposium on "Novel Strategies for Targeted Prevention and Treatment of Cancer" on December 19-20, 2008. The symposium organized at the School of Life Sciences, Jawaharlal Nehru University (JNU), New Delhi, India, and Received BEST PAPER AWARD

12 Harish Nayaka, M.A., Sathisha, U.V. and Dharmesh,S.M. (2008): Pectic polysaccharide from Swallow Root –SRPP inhibits B16F10 cell Induced Lung Metastasis in Swiss Mice; Down regulation of Galectin-3. Presented at International Symposium on "Novel Strategies for Targeted Prevention and Treatment of Cancer" on December 19-20, 2008. held at The School of Life Sciences, Jawaharlal Nehru University (JNU), New Delhi, India

13 Smitha Jayaram and Dharmesh,S.M., (2008): Inhibition of Phosphogluco

Isomerase activity by andrographolide and phenolic acids from Andrographis Serpyllifolia. Presented at “International Symposium on Novel Strategies for Targeted Prevention and Treatment of Cancer”, December 19-20, 2008. held at The School of Life Sciences, Jawaharlal Nehru University (JNU), New Delhi, India

14 Srikanta, B.M, Sathisha, U.V, Dharmesh,S.M. (2008): Ulcer healing

mechanism of a multi-step active phenol-bound pectic polysaccharide from swallow root - Decalepis hamiltonii. Presented at 6th International Food Convention, IFCON 2008”, December 15-19, 2008, held at CFTRI, Mysore.

15 Lakshminarayana, R,, Aruna, G,, Sathisha, U.V., Dharmesh,S.M. and

Bhaskaran, V. (2008): Antiproliferative property of oxidized lutein in human cervical carcinoma cell lines. Presented at “6th International Food Convention, IFCON 2008”, December 15-19, 2008, held at CFTRI, Mysore.

16 Vismaya, Sindhu, R, Srikanta, B.M, Dharmesh, S.M. and Sindu kanya T.C. (2008): Antiulcer potential of karanjin; a furanoflavonoid from Karanja seed oil. Presented at 77th Annual meeting of SBC(I), December 18-20th 2008, held at IITM, Chennai.

17 Mani, G., Dharmesh,S.M. and Renu Agrawal. (2008): Preliminary studies to explore antimicrobial activity of lactic acid bacteria to combat shigellosis. Presented at 77th Annual Session and Symposium of National Academy of

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Sciences, India (NASI), on Novel approaches for food and Nutritional security, December 6 – 8, 2007, held at CFTRI, Mysore,

18 Srikanta, B.M., Ashwini and Dharmesh, S.M. (2007): Determination of antiulcerogenic potential in green chilli (Capsicum annuum). Presented at 77th Annual Session and Symposium of National Academy of Sciences, India (NASI), on Novel approaches for food and Nutritional security, December 6 – 8, 2007, held at CFTRI, Mysore,

19 Srikanta, B.M. Siddaraju M.N. and Dharmesh, S.M. (2006): Swallow root pectic polysaccharide: its efficacy as a potential antiulcer source against ulcer, International symposium on Building leadership skills in food and nutrition essential for national development, CFTRI, Mysore, June 23- 25, 2006.

20 Siddaraju, M.N, Srikanta, B.M. and Dharmesh, S.M. : “Ginger pectic polysaccharide: its efficacy as a potential alternative source against ulcer” presented at International Conference On Ethnopharmacology And Alternative Medicine, Amala Cancer Research Center, Thrissur, Kerala. Feb. 2-4, 2006.

21 Siddaraju.M.N, Smitha.J and Dharmesh, S.M. “Multi-step ulcer intervention in vitro by Mango ginger (Curcuma amada)” 7th Food Convention (ICFOST), NIMHANS, Bangalore, Dec. 5-6, 2006.

22 Siddaraju.M.N, Smitha.J and Dharmesh, S.M. “Antiulcer activity of ginger phenolic fractions in vitro”7th Food Convention (ICFOST), NIMHANS, Bangalore, Dec. 5-6, 2005. BEST POSTER AWARD.

23.Siddaraju M.N Srikanta B.M. and Dharmesh, S.M. (2006): Ginger pectic polysaccharide: its efficacy as a potential alternative source against ulcer, International Conference On Ethnopharmacology And Alternative Medicine, Amala Cancer Research Center, Thrissur, Kerala. Feb. 2-4, 2006.

24. Smitha, J., Harish Nayaka M.A., and Dharmesh, S,M (2004): Anticulcer components from dietary sources. Presented at the international symposium on toxins and venoms, held at the Department of Biochemistry, University of Mysore, Mysore during January 25 & 26, 2004 - Paper received a MERIT AWARD.

25. Smitha, J, Sathisha, U.V., Harish Nayaka M.A,and Dharmesh, S.M. (2004): Galectin 3,a potential tool to evaluate dietary antimetastatic compounds.. Presented at the Indo-Australia international symposium in international meeting on Biomaterials and Biotechniques, held at Kasturba Medical College, Manipal, India. During February 13,

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26. Siddaraju, M.N., Harish Nayaka.M.A., Smitha.J. and Dharmesh, S.M. (2003): Cytoprotective effect of spice antioxidants. Presented in 5th International food convention held during December 5-8, at CFTRI, Mysore, India.

27. Kazi , A.N Dharmesh, S.M., Rajan, M.G.R., Salimath, P.V. (2003) : Development of a sensitive immunoassay to detect Diabetic Nephropathy. Presented in 5th International food convention held during December 5-8, at CFTRI, Mysore, India.

28. Harish Nayaka, M.A., Siddaraju, M.N and Dharmesh, S.M. (2003): Multi-potent antioxidant components in Decalepis hamiltonii. Presented at the international conference on the role of free radicals and antioxidants in health and disease, held during 10-12,at Lucknow, India.

29. Siddaraju.M.N., Smitha.J., Sathisha.U.V. and Dharmesh,S.M. (2003): Potential gastric ulcer preventive polysaccharide from Ginger (zingiber officinalis). Presented in XVIIIth Carbohydrate conference held during November 5-7 at Kolkata, India.

LECTURES:

Invited Speaker: Invited as a special guest to deliver valedictory lecture on

Current trends in cancer Research at Post –graduate college of Biochemsitry

and Biotechnology in 2010; Invited as lead speaker at the 2nd National Women’s

Science Congress held at the All India Institute of Speech and Hearing during

November 7-9 2009. To deliver lecture and demonstrate, vitamin analysis by

HPLC, during short term training programme during May 2009; Delivered

Lecture series on Cancer and Stem cells at Yuvarajas college, Mysore, October

– November 2009; Delivered a National Science day Lecture at the Department

of Microbiology, Shankarghatta on 28th Feb 2010 on Advances in Cancer

Research; to deliver lecture and demonstrate Animal Cell culture techniques

during short term training programme during 2nd April 2008 at CFTRI, to deliver

and participate in the discussion on Trends in Cancer Research furture scenario

during March 12, 2008.

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Item 1.7: Training programmes, workshops, degree programmes including

theses supervised:

a) Involved in training programme - on “Work Life Balance for Women

Scientists of CSIR” held from 3–5 March, 2010 at HRDC, Ghaziabad”, New

Delhi.

b) Involved in the training programme on Vitamin Analysis by HPLC at

CFTRI, Mysore, 2009.

c)Involved in training programme - on NABL auditing and awareness, CFTRI,

Mysore.

TEACHING AND GUIDANCE TO POST-GRADUATE AND PH.D PROJECTS: Teaching and Guidance to M.Phil, Biotechnology and Ph.D Projects Teaching Experience in Biotechnology and Biochemistry M.Sc Courses:

Biochemical Techniques, Immunology, Immunotechnology, Cell Biology,

Metabolism, Macromolecules, Tumor Biology and Microbiology.

Guidance to M.Phil and Biotechnology/Biochemistry projects:

1. Nithash and Dharmesh, S.M: January 2011 till May 2011: Tyrosinase inhibitory effect of dietary pectic polysaccharide.

2. Praveen and Dharmesh, S.M: January 2011 till May 2011: Galectin-Inhibitory property of dietary pectic polysaccharide.

3. Nirlep and Dharmesh, S.M : July 2010 till July 2011: Antiulcer potentials from dietary sources; pharmaceutical formulation,

4. Priya and Dharmesh, S.M: M.Sc in Food Science, January to April 2010 Bioactive Compounds from Potato (Solanum tuberosum),Ginger (Zingiber officinale)

5. Shwetha, K.V and Dharmesh, S.M: M.Sc in Biotechnology, February to May 2010: Bioactive Compounds From Dietary Sources – Comparative Evaluation.

6. Amshumantha, S.J., Charulatha, G, Uma, T.M and Dharmesh, S.M: B.E. in Biotechnology, January to April 2010: Antioxidants, antiulcer and anticancer compounds from dietary sources.

7. Balakrishna Naidu, M.Y and Dharmesh, S.M: M.Sc Biotechnology, January till March 2010: Potential Nutraceuticals inCurry Laves (Murraya Koenigii) & Sweet Orange (Citrus sinesis).

8. Leela Bai, S and Dharmesh, S.M: M.Sc in Biotechnology, January till March 2010: Potential Nutraceuticals Fenugreek (Trigonella foenum-graecum ) and Mangosteen (Garcinia mangostana L.).

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9. Soujanya, J and Dharmesh, S.M: M. Pharma, July 2009 till March 2010: Gastroprotective, Anti-inflammatory and Cardioprotective Properties of Benincasa hispida (cucurbitaceae).

10. Kruthi, and Dharmesh, S.M: M.Sc in Biotechnology during March till June 2009 on “Anticancer and antiulcer compounds from different fractions in Grapes.

11. Marjan Askari and Dharmesh, S.M: M.Sc Biotechnology, March to May 2007: Anticancer compounds from a selected dietary sources.

12. Ghousia Begum and Dharmesh, S.M: M.Sc Biotechnology, January to April 2007: Health beneficial compounds from dietary sources.

13. Nelamma and Dharmesh, S.M: M.Sc Biotechnology, February to May 2007: Detection of anti-cancer compounds from dietary sources.

14. Ashwini, S.M and Dharmesh, S.M: M.Sc Biotechnology, February to May 2007: Ientification of ulcerogenic and antiulcerogenic components from dietary sources.

15. Leili Farajzeh and Dharmesh, S.M: M.Sc Biotechnology, January 2006 to March 2006: Isolation and Characterization of Anticancer Compounds from Swallow Root (Decalepis hamiltonii).

16. Meghana and Dharmesh, S.M: M.Sc Biotechnology, January 2006 to March 2006: Isolation and Characterization of Antiulcer compounds from Mango Ginger (Curcuma amada).

17. Damaris and Dharmesh, S.M. (2004): Cytoprotective bioactive compounds from dietary soruces.

18. Diya Jhon., and Dharmesh, S.M. (2004): Inhibitor of PhosphoglucoIsomerase from Edible root extract.

19. Poonghuali, K., and Dharmesh, S.M. (2003): Potential ulcer preventive compounents from dietry sources.

20. Mahesh., and Dharmesh, S.M. (2002): Bioactive copounds from edible root.

21. Yougendra Nayaka and Dharmesh, S.M. (2001): Anticancer and antiulcer compounds from spices; demonstration by in vitro and in vivo models.

22. Bharghavi, and Dharmesh, S.M. (2000): Bioactive compounds from dietary sources.

23. Mamatha, Dharmesh, S.M., & Shetty, H.S [1999]: Oxidative crosslinking and its role in downy mildew disease resistance in pearl millet.

24. Poornima, Dharmesh, S.M., and Shetty, H.S. [1999]: Molecular mechanisms involved in pseudomonas fluorescens induced biocontrol in pearl millet.

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25. Nandini, S., Dharmesh, S.M., Prakash, H.S., & Shetty, H.S. [1998] : Molecular understanding of carcinogenesis and development of assay for the detection of malignant cells.

26. Satish, N.B., Dharmesh.,S.M., Krishnappa, M., & Shetty, H.S. [ 1998 ] : Biochemical and serological characterization of Ralstonia solanacearum of tomato isolates.

27. Sarala, Dharmesh, S.M., Krishnappa, M., & Shetty, H.S [1997]: Molecular basis of clavibacter michiganensis infection in tomato; its prevalence in Karnataka.

28. Vijayananda, G.K., Dharmesh, S.M., Prakash, H.S., & Shetty, H.S. [1996]: Bacterial blight caused by Xanthomonas campestris pv. Cyamopsidis in clusterbean.

29. Rosaline, R., Dharmesh, S.M., Krishnappa, M., & Shetty, H.S. [1995]: Pathogenecity basis and developmental regulation of bacterial wilt of solanaceous crops by Burkholderia solanacearum.

30. Thoyajaksha, Dharmesh., S.M., Niranjana, S.R., & Shetty, H.S. [1995]: Mechanism of Beuveria bassiana induced control of coffee berry borer in coffee plant.

31. Umashankar, Dharmesh., S.M., & Shetty, H.S. [1995]: Role of Hydroxy proline- rich glycoprotein in downy mildew disease resistance in pearl millet.

32. Umesha, S., Dharmesh., S.M., & Shetty, H.S. [1994 ]: Molecular basis of downy mildew disease resistance in pearl millet.

33. Sarala, Dharmesh., S.M., & Shetty, H.S. [1999]: Clavibacter michiganensis infection in tomato; Molecular understanding of bio-controlling of the disease.

34. Madhu, D., Dharmesh, S.M., Prakash. & Shetty, H.S [1999]: Proton-ATPase activity and its involvement in downy mildew disease resistance in pearl millet.

35. Nalini, M.S., Dharmesh, S.M., & Prakash, H.S. [1998]: Isolate variation and seed transmission of bean common mosaic virus in French bean.

36. Rajeshwari, N., Dharmesh, S,M., Krishnappa, M & Shety, H.S. [1999]: Development of sensitive ELISA in the detection of bacterial wilt pathogen of tomato.

37. Chitra, T.R., Dharmesh, S.M., & Prakash, H.S. [1995]: Biochemical basis of tobamovirus infection in tomato.

38. Veena, M.S., Dharmesh, S.M., & Shetty, H.S. [1995]: Development of Immunoassays and study of Xantohmonas Oryzae pv. Oryzae, in paddy.

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Guidance to Ph.D projects [Biochemical, Immunological and Molecular aspects]:

1. Harish Nayaka, M.A: Studies on antioxidants and antimetastatic compounds from dietary spices. (Degree Awarded)

2. Siddaraju, M.N: CSIR – SRF: Antiulcer and Anticancer bioactive compounds

from Zingeberaceae Species. (Degree Awarded)

3. Smitha, J – CSIR – SRF: Identification of multivarietal and multi-mechanistic anticancer components from dietary sources. (Thesis under preparation)

4. Sathisha, U.V. – ICMR – SRF: Modulation of biological functions of galectin-3

by dietary galectin- inhibitors in in vitro and in vivo models (Degree Awarded)

5. Srikanta, B.M – CSIR – JRF: Mechanism of action of multi – potent ulcer blockers in in vitro and in vivo models. (Thesis submitted)

6. Sindhuja – Uiverisity fellow – JRF: Studies on the role of dietary galectin

inhibitor on galectin and galectin-binding protein mediated metastasis

7. Kavitha – CSIR – SRF: Understanding on the mechanism of action of anti-ulcer compounds from cabbage and potato.

8. Sabeeta – DBT - JRF: Modulation of metastasis by low molecular weight galectin-inhibitors from Tomato (Solanum lycopersicum)]

9. Harsha – CSIR - SRF: Antiulcer potentials of Turmeric (Curcuma longa)

10. Meghashree – CSIR – RA: Determination of potency of dietary Helicobacter pylori inhibitors against cancer and ulcer in in vitro and in vivo models employing glycan and microarray techniques

11. Hasitha- UGC JRF : Molecular mechanisms in metastasis; modulation by

dietary sources

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