Parallel and Simultaneous Dispensing of Nanoliter

Volumes for the Production of BioChips and Diagnostic

Tests

Bellows Dispensing Technology

Bottom valves are opened

Dispenser is ready for dispensing

Top valves are closed

Bellows are filled and expanded

Connections to individual reagent reservoirs

Bottom valves

Top valves

Bellows

Dispenser needles

bottom valves opened

Lilliput Plate is positioned

top valves are closed

Bellows are filled and expanded

Connections to individual reagent reservoirs

bottom valves opened

Dispensing starts

top valves are closed

mechanical compressionof the bellows

Connections to individual reagent reservoirs

bottom valves opened

trackdown of the Lilliput chip

Connections to individual reagent reservoirs Dispensing ready

top valves are closed

mechanical compressionof the bellows

bottom valves opened

Removal of theLilliput chip

top valves are closed

mechanical compressionof the bellows

removal of the Lilliput chip

Connections to individual reagent reservoirs

Valve operation

top valves are closed

Connections to individual reagent reservoirs

bottom valves are closing

bottom valves are closing

Valve operation

top valves are closed

Connections to individual reagent reservoirs

bottom valves are closing

Valve operation

top valves are closed

Connections to individual reagent reservoirs

bottom valves are closing

Valve operation

top valves are closed

Connections to individual reagent reservoirs

bottom valves closed

Valve operation

top valves are opening

Connections to individual reagent reservoirs

bottom valves closed

Valve operation

top valves are opening

Connections to individual reagent reservoirs

bottom valves closed

Valve operation

top valves are opening

Connections to individual reagent reservoirs

bottom valves closed

Bellows ready for refill

top valves are open

Connections to individual reagent reservoirs

bottom valves closed

Bellows ready for refill

top valves opened

Connections to individual reagent reservoirs

bottom valves closed

Bellows refill

top valves opened

Connections to individual reagent reservoirs

Bellows expand

bottom valves closed

Bellows refill

top valves opened

Connections to individual reagent reservoirs

Bellows expand

bottom valves closed

Bellows refill

top valves opened

Connections to individual reagent reservoirs

Bellows expand

bottom valves closed

Bellows refill

top valves opened

Connections to individual reagent reservoirs

Bellows expand

bottom valves closed

Bellows refill

top valves opened

Connections to individual reagent reservoirs

Bellows expand

bottom valves closed

Bellows refill

top valves opened

Connections to individual reagent reservoirs

Bellows expand

bottom valves closed

Bellows refill

top valves opened

Connections to individual reagent reservoirs

Bellows expand

top valves are closing

bottom valves areopening

Valve operation Connections to individual reagent reservoirs

Valve operation Connections to individual reagent reservoirs

top valves are closing

bottom valves areopening

bottom valves opened

Dispenser ready for operation

top valves closed

Connections to individual reagent reservoirs

SpecificationsSpecifications

Accuracy: VC < 2%

Volumes: 200nl up to 2000nl [2µl – 100µl]

Bellows size: 3µl up to 2ml

Channels: 1 to 384 in parallel

Reagents: 1 to 192 different solutions simultaneously

Operation Modes: - constant dispensing from reservoirs- aspirate and dispense

Outlet: - stainless steel needles (teflon or paralene coated)- pipette tips

Movement Control: Stepper motor. Resolution 14,9 steps to squeeze 1 nl

References: - manufacturing of 384 well plates - manufacturing of Lilliput Chip- manufacturing of 96 well Micro Titer Plates

Accuracy: VC < 2%

Volumes: 200nl up to 2000nl [2µl – 100µl]

Bellows size: 3µl up to 2ml

Channels: 1 to 384 in parallel

Reagents: 1 to 192 different solutions simultaneously

Operation Modes: - constant dispensing from reservoirs- aspirate and dispense

Outlet: - stainless steel needles (teflon or paralene coated)- pipette tips

Movement Control: Stepper motor. Resolution 14,9 steps to squeeze 1 nl

References: - manufacturing of 384 well plates - manufacturing of Lilliput Chip- manufacturing of 96 well Micro Titer Plates