PANFLAVIVIRUS MOSAIK … MOSAIK IMMUNOFLUORESCENCE. EUROPEAN CENTRE FOR DISEASE PREVENTION ......
Transcript of PANFLAVIVIRUS MOSAIK … MOSAIK IMMUNOFLUORESCENCE. EUROPEAN CENTRE FOR DISEASE PREVENTION ......
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PANFLAVIVIRUS MOSAIK IMMUNOFLUORESCENCE
Trainer: Dr. Matthias NiedrigAssistants: Dr. Nadine Litzba/Dr. Katharina Achazi
Dengue Diagnosis Course‐ 23rd to 27th Jan 2012
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PANFLAVIVIRU
S MOSAIK IM
MUNOFLU
ORESCEN
CE
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Overview of biochips on each reaction field
Inverted overview of biochips as you see
through our microscope
control YFV JEV
DENV4
DENV3
DENV1
DENV2
WNV
TBEV
TBEV DENV2 DENV3
WNV
JEV
DENV1
YFV
DENV4
control
Overview of biochips on each reaction field
PANFLAVIVIRU
S MOSAIK IM
MUNOFLU
ORESCEN
CE
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How to read the slides:
• Rate the fluorescence from 0 (= no fluorescence) up to 5 (= strongest fluorescence possible)
• Calculation of the titre:1:10 dilution:
rate of 1 = 1:102 = 1:323 = 1:1004 and 5 = >1:100
1:100 dilution:1 = 1:1002 = 1:3203 = 1:10004 and 5 = > 1:1000
etc.
PANFLAVIVIRU
S MOSAIK IM
MUNOFLU
ORESCEN
CE