PANFLAVIVIRUS MOSAIK … MOSAIK IMMUNOFLUORESCENCE. EUROPEAN CENTRE FOR DISEASE PREVENTION ......

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1 PANFLAVIVIRUS MOSAIK IMMUNOFLUORESCENCE Trainer: Dr. Matthias Niedrig Assistants: Dr. Nadine Litzba/Dr. Katharina Achazi Dengue Diagnosis Course23 rd to 27 th Jan 2012

Transcript of PANFLAVIVIRUS MOSAIK … MOSAIK IMMUNOFLUORESCENCE. EUROPEAN CENTRE FOR DISEASE PREVENTION ......

Page 1: PANFLAVIVIRUS MOSAIK … MOSAIK IMMUNOFLUORESCENCE. EUROPEAN CENTRE FOR DISEASE PREVENTION ... Microsoft PowerPoint - mosaik presentation.ppt [Kompatibilitätsmodus]

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PANFLAVIVIRUS MOSAIK IMMUNOFLUORESCENCE

Trainer: Dr. Matthias NiedrigAssistants: Dr. Nadine Litzba/Dr. Katharina Achazi

Dengue Diagnosis Course‐ 23rd to 27th Jan 2012 

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PANFLAVIVIRU

S MOSAIK IM

MUNOFLU

ORESCEN

CE

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Overview of biochips on each reaction field

Inverted overview of biochips as you see 

through our microscope

control YFV JEV

DENV4

DENV3

DENV1

DENV2

WNV

TBEV

TBEV DENV2 DENV3

WNV

JEV

DENV1

YFV

DENV4

control

Overview of biochips on each reaction field

PANFLAVIVIRU

S MOSAIK IM

MUNOFLU

ORESCEN

CE

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How to read the slides:

• Rate the fluorescence from 0 (= no fluorescence) up to 5 (= strongest fluorescence possible)

• Calculation of the titre:1:10 dilution:

rate of 1 = 1:102 = 1:323 = 1:1004 and 5 = >1:100

1:100 dilution:1 = 1:1002 = 1:3203 = 1:10004 and 5 = > 1:1000

etc.

PANFLAVIVIRU

S MOSAIK IM

MUNOFLU

ORESCEN

CE