BCCM/GeneCornerA plasmid collection at your service

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GeneCorner

P4EU_04jul2018Martine VanhouckeBCCM/GeneCorner manager

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GeneCorner

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� Do you want to share yourvaluable constructs withother scientists?

� Are you looking for plasmidsor related host strains?

� Do you want to secure yourplasmids, cell lines or othergenetic resources?

� Are you looking for otherplasmid-related services?

GeneCorner

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BCCM/GeneCornernon-profit services

by scientistsfor scientists

Major active European plasmid collection

GeneCorner

BCCM/GeneCorner evolved since 1977 towards a unique plasmid collection, assuring plasmid authenticity and validated information

19772018

History

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GeneCorner

Embedding

Faculty of Sciences

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Belgian Co-ordinated Collections of Micro-organisms (BC CM)

� Non-profit consortium consisting of seven entities:� BCCM/IHEM biomedical fungi & yeasts� BCCM/GeneCorner plasmids & libraries� BCCM/LMG bacteria� BCCM/MUCL (agro)industrial fungi & yeasts � BCCM/DCG diatoms� BCCM/ITM mycobacteria� BCCM/ULC polar cyanobacteria

� More than 100.000 well documented and authenticated resources

7 biological resource centres

Coordinated by a central team at BELSPO

Funded by the Belgian Science Policy Office - BELSPO

GeneCorner

Fundamental biotechnological research:- inflammation- immunity- cancer

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GeneCorner

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� Do you want to share your valuableconstructs with other scientists?

Your incentives?

� Circulation of biological material is essential for scientific progress� often hindered:

� busy research schedules, resulting in variable qual ity and delays in shipment� high turnover of scientists: inefficient storage an d high risk of loosing plasmids and associated informat ion

� Deposit in the public GeneCorner Collection!

GeneCorner

pGSV4/WRKY23/CDKA;1S

GeneCorner

GeneCorner

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� Do you want to share your valuableconstructs with other scientists?

Your incentives?

� Circulation of biological material is essential for scientific progress

� Pragmatic solution for long-term preservation

� Storage under quality-controlled conditions

� Easy access to quality-controlled biological materi al for the broader scientific community

� Meet the concerns of the debate on scientific integ rity and research data management

� Accession codes for deposits need to be provided in the pap er

� The visibility of the depositor increases:� depositor’s name + publication mentioned in the cat alogue record

� increased citation index

� Free service, simple procedure

� Deposit under the conditions of a Material Accessio n Agreement (MAA)

� Deposit in the public GeneCorner Collection!

GeneCorner

Deposit in the transit collection is possible before pub lication!

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� Are you looking for plasmids or related host strains?

Browse our online catalogues: > 213.800 biological re sources

� ± 4.000 plasmids for cloning, expression, mouse genet ic engineering, reporter genes, …

� ± 60 plasmid-related host strains

� Libraries and library clones� the genome-wide SIGMA MISSION TRC1 and part of the TRC1.5 human and mouse shRNA libraries

more than 176,000 pre-cloned shRNA constructs for R NAi-mediated gene silencing

at strongly reduced prices as compared to the SIGMA prices

� the human ORFeome v8.1 entry plasmidscentral resource of over 12,000 single-colony, sequ ence-verified human ORFs cloned in pDONR223

� Distribution under the conditions of a Material Tra nsfer Agreement

� Distribution in return for a small fee covering min imal costs

� BCCM/GeneCorner distribution service!

You can’t find what you need � contact us to have a look in the transit collection .

GeneCorner

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� Do you want to secure your biologicalmaterial?

� BCCM/GeneCorner confidential depositservices!

� Safe deposit (SD) collection� Back-up on a second location, incl. quality controls

� Patent deposit collection� Compulsary part of your patent process

� According to the Budapest Treaty

� IDA status from WIPO

� Storage third party’s biological material (STPM)� Back-up on a second location, excl. quality controls

� Not online catalogued

� Charged (minimal fee)

� Distribution to third parties� Safe deposit and STPM samples: depositor’s approval required

� Patent deposit samples: governed by patent law

GeneCorner

BCCM/GeneCorner accepts not only plasmids but also cell lin es and hybridoma’s.

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� Are you looking for other plasmid-relatedservices?

� Scientific services

� Plasmid profile analysis of non-pathogenic bacterialstrains

� Sequence analysis of non-characterised plasmids

� Training

GeneCorner

Quality management

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QMS according to ISO 9001:2015

GeneCorner

Quality management• Quality-controlled preservation

– 2 different preservation methods– controlled access to the freezers– continuous temperature registration– call processor– detailed alarm instructions

• Biological material subjected to quality controls:– purity– viability– authenticity insert + backbone: currently analysis restriction enzyme pattern; in 2019: sequence

analysis

• Data processing according to European norms– Minimal data set for non-core plasmids– Full data set for core plasmids

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� detailed datasheet� sequence file� standardized VNTI circular map� link to PUBMED� link to sequence databases� authenticity test

Daily updated online catalogue

GeneCorner

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BCCM website: http:/bccm.belspo.be /

IRC website: http:/www.irc.ugent.be/� Research � Core facilities � BCCM-GeneCorner Plasmid Collection

UGent website: http://www.genecorner.ugent.be/

BCCM/GeneCorner booth at P4U

� Do you still want more info?

GeneCorner

BCCM/GeneCorner, a reliable partner for supporting your biotechnological research and activities.

Tel: 32 9 33 13 843bccm.genecorner@ugent.be

GeneCorner

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Rudi Beyaertpromoter

Martine Vanhouckemanager

Anneleen Olbrechtscurator public collectionLIMS responsible

Wouter De Schamphelairedata processorcurator safe and patent depositsIT responsibleexternal communication manager

Benedicte Sanctobinadministrative collaborator

Kübra Alcitechnician

GeneCorner

Thank you for your attention

The GeneCorner team