Concerted Dynamic Motions of an FABP4 Model and Its Ligands ...
P2-98 The influence of birth weight on FABP4 and muscle triacylglyceride levels in skeletal muscle...
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Transcript of P2-98 The influence of birth weight on FABP4 and muscle triacylglyceride levels in skeletal muscle...
S156 Posters
Results: Maternal HF diet with or without statins treatment
resulted in significant reduction in the femur and tibia length in
male offspringp < 0. Significant reductions in bone strength were
observed in offspring from HF+statin damsp < 0. Offspring from HF-
and C-fed dams and themselves fed HF diet showed significant
reductions in bone pliabilityp < 0. Offspring from HF dams with
or without statin showed reduced stress at fracture and increase
femoral bone marrow adiposityp < 0. & We also found reduced
trabecular thickness and increased bone area/volume ratiop < 0 in
HF-fed offspring from C or HF+statin dams.
Conclusions: These data show alteration of male offspring bone
structure related to both maternal and offspring dietary fat levels.
Given increasing adiposity in the developed world, such bone
alterations require further investigation.
P2-96 Alteration of fetal bone structure by a maternal
low protein diet
S.A. Lanham1 *, C. Roberts1, S.P. Bagby2, C. Cooper1,
R.O.C. Oreffo1. 1Bone and Joint Research Group, Developmental
Origins of Health and Disease, University of Southampton,
Southampton, SO16 6YD, UK, 2Division of Nephrology &
Hypertension, Oregon Health and Science University, Portland,
USA
E-mail: [email protected]
Introduction: Epidemiological studies suggest skeletal growth is
programmed during intrauterine and early postnatal life. We
hypothesize that age-related decrease in bone mass has, in part, a
fetal origin and investigated this using an ovine model of maternal
protein insufficiency.
Methods: Pregnant pigs fed on low (1% w/w) protein diet or control
protein diet (14% w/w) for the 3rd trimester. Fetal sample were
taken on day 113 of 115 day gestation. Femur and vertebra samples
were analysed using micro-CT.
Results: The restricted group offspring showed increased density
of the trabecular bone and apparent reduction in the proportion
of cartilage in the proximal femur, although no differences were
found in trabecular structure. No significant differences were found
in either the bone density or structure in the femoral head. Also,
no differences were found in the vertebral bone density, but the
vertebral trabeculae in the restricted diet group offspring were
spaced further apart indicated by increased trabecular spacing,
fewer trabeculae per mm and a reduced bone volume/total volume
ratio.
Conclusions: Offspring from mothers fed a restricted protein diet
during pregnancy displayed significant differences in bone structure
and density at various sites. These differences result in altered
bone characteristics indicative of accelerated bone formation.
These results indicate the need to understand the key role of the
nutritional environment in early development on programming of
skeletal development and consequences in both early and later life
for altered development.
P2-97 Birth weight, current body composition and grip
strength in an aboriginal birth cohort
G.R. Singh*, S.M. Sayers. Menzies School of Health Research,
School of Advanced Studies, Charles Darwin University, Australia
Low birth weight has been linked to lower lean body mass
and increased abdominal obesity in later life. Grip strength
(Dynamometer) is a measure of muscle strength and indirectly of
muscle mass.
Aims: To study grip strength and its relationship to size at birth and
current size.
Study design: Cross-sectional snapshot in a longitudinal study.
Subjects: 316 young adults between 16 20 years of age; wave 3 of
the cohort study.
Outcome measures: Grip strength (maximal value of 3 squeezes of
the dominant hand), birth weight (BW), current body size (weight,
height, BMI, waist circumference), fat percentage and fat free mass
(FFM) by Bioelectric Impedance Assessment (TBF-310GS, Tanita).
Results: The grip strength showed a significant positive association
with BW (2.7 [1.1, 4.3]; p = 0.001), FFM (0.7 [0.6, 0.7]; p� 0.001)
and BMI (1.9 [1.3, 2.5]; p� 0.001) and a negative relationship with
percentage of fat ( 0.09 [ 0.2, 0.0001]; p = 0.05). A 1 kg increase
in birth weight corresponded to 1.8 kg (0.27, 3.3; p = 0.21) increase
in FFM in females and a 3.1 kg (1.2, 4.9; p = 0.001) in males. The
association remained significant after adjusting for age, current BMI
and sex.
Conclusions: Low birth weight affects body composition resulting
in lower FFM and manifests as lower grip strength. This is in similar
to findings in previous studies. Grip strength is a good indicator of
overall muscle mass and an important predictor of future physical
function, cognitive function and age related diseases, and thus
may have practical implications in assessing and modifying future
quality of life.
P2-98 The influence of birth weight on FABP4 and muscle
triacylglyceride levels in skeletal muscle of neonatal
pigs
P.J. Williams1, J.C. Litten2, A.M. Corson3, L. Clarke3,
M.E. Symonds1 *, A. Mostyn1. 1Centre for Reproduction and
Early Life, Institute of Clinical Research, University Hospital,
Nottingham, NG7 2UH, UK, 2School of Agriculture, Policy &
Development, University of Reading, RG6 6AR, UK, 3Faculty of
Natural Sciences, Imperial College London, Wye, Kent, TN25 5AH,
UK
E-mail: [email protected]
Birth weight has important consequences on skeletal muscle
development. The intracellular transport of fatty acids from the
plasma membrane is performed by members of the fatty acid-
binding protein (FABP) family. FABP’s may also regulate lipid
metabolism and numerous other cellular processes.
Aims: To examine if birth weight influences the expression of FABP4
in skeletal muscle (SM) at days 7 and 14 of postnatal life.
Study design: FABP4 expression was quantified by real-time
PCR. Results are expressed as mean values and standard errors
normalised to 18S. SM triacylglyceride (SM-TAG) was assessed using
a commercial assay following Folch extraction.
Subjects: Piglets from eleven litters were ranked according to
birth weight and three from each litter were assigned to small
(S; n = 11), normal (N; n = 11) or large (L; n = 11) groups. Animals
were humanely euthanased on day 7 (n = 15) or 14 (n = 18) of age
and SM sampled.
Outcome measures: Variation in markers of lipid metabolism with
birth weight.
Results: At day 14 FABP4 abundance was decreased in both
S (2.0[0.4]) and L (2.5[0.7]) compared to N pigs (9.2[1.3]);
P< 0.0001. Furthermore, in N pigs FABP4 expression at day 14 was
positively related with SM-TAG (P= 0.037). This correlation was not
displayed in either S or L pigs.
Conclusions: These results indicate altered lipid metabolism due
to dysregulation of FABP4 within the muscle of both offspring with
large and small birth weights. Further work is needed to examine if
these changes persist into adult life where they may be associated
with the onset of the metabolic syndrome.
P2-99 Intra-uterine growth retartion in piglets is
associated with an altered distribution of
leptin receptor expression within hypothalamic
structures
L. Attig1,2, O. Rampin1, P.M. Anton-Gay2, I. Gourdou1,
J. Djiane1, L. Abdennebi-Najar2 *. 1NOPA, UMR 1197, INRA, Univ
Paris-Sud, Jouy-en-Josas, 78350, France, 2Institut Polytechnique
Lasalle-Beauvais, 60026, France
In pig, intrauterine growth retardation (IUGR) resulted in low
birth weight and increased adiposity in adult life. It has been
demonstrated that leptin plays a crucial role in the development
of the hypothalamic circuitry involved in food intake regulation.