OZ Biosciences 3D TRANSFECTION
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Applications Tissue engineering, tissue regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation, colonization, neurite growth.... Highly efficient on cell lines & primary cells 3D-Fect™ has been tested on a wide range of cells and achieves high transfection efficiencies. It is suitable for all culture conditions and does not affect matrice colonization and cell growth. 3D-Fect™ is a novel reagent, based on an innovative technology, specifically developed to directly transfect cells cultured in 3D scaffold. 3D matrices not only add a third dimension to cells' environment, they also allow creating significant differences in cellular characteristics and behavior. In this way, scaffold-based 3D matrices combined with 3D-Fect/DNA complexes are colonized by cells to be transfected in a more natural environment. 3D-Fect™ a new outlook for your cells ! Fig. 3: HMEC-1 transfected with 2, 3 or 4μg of DNA in a collagen-derived 3D-Scaffold. l Highly efficient on cell lines and primary cells l Specific for 3D scaffolds (3D-Fect TM ) or 3D hydrogels (3D-FectIN TM ) l All type of nucleic acids: plasmid DNA, siRNA, shRNA... l Applicable to a broad range of cells 3D-Fect™ & 3D-FectIN™ features l Completely biodegradable l Serum compatible l Long term protein expression l Simple, ready-to-use & rapid Please visit www.ozbiosciences.com for more results 3D-FectIN™ a novel perspective for your cells ! Suitable for all nucleic acids This reagent is applicable for DNA, shRNA and siRNA transfection. The protocol is simple and rapid. Fig. 2: Cells transfected on various 3D-scaffolds. a. HEK-293T - b. HeLa - c. MEF - d. Neural Stem Cells Fig. 1: Cells transfected in a collagen-based 3D-Scaffold. Alkaline Phosphatase (AP) Secretion Fig. 6: NIH-3T3 transfected in a collagen-derived hydrogel with 3D-FectIN and others reagents. Transfection Efficiency Comparison in NIH-3T3 cells Fig. 4: Cells transfected in hydrogels. a. Raw 264.7 - b. HMEC-1 - c. HEK-293T - d. Neural Stem Fig. 5: Cells transfected in a collagen-based hydrogel with 3D-FectIN. Gentle to cells 3D-Fect™ is biodegradable and allows high cell viability. It is serum compatible, thus no medium change is required. Long term protein expression 3D-Fect™ allows 3D trangene expression studies in in vivo like conditions over a long time period. Specific for 3D-Scaffolds Examples of 3D-Scaffolds successfully tested: Outperforms competitors Higher efficiency in term of number of transfected cells and transgene expression than other commercially available reagents. Please visit www.ozbiosciences.com for more results Versatile and universal It is adapted to all culture conditions and suitable for all nucleic acids (DNA, shRNA, siRNA...). 3D-FectIN™ procedure is easy and straightforward. Great efficiency on a variety of cells 3D-FectIN™ is highly efficient and suitable for any cells: immortalized and primary. Transgene expression is high and long-lasting. Outperforms competitors 3D-FectIN™ achieves superior efficiency than other reagents Specific for any hydrogels supporting 3D culture Examples of 3D-Hydrogels successfully tested: 3D-FectIN™ is a new transfection technology specially engineered to directly transfect cells cultured in 3D hydrogel. 3D-FectIN™ adds a third dimension to cell culture! 3D matrices allow cells to grow in micro-environment that more closely mimics the 3D environment encountered by cells in vivo. Thus, hydrogel-based 3D matrices combined with 3D-FectIN/DNA complexes allow cells to be directly transfected in more natural surroundings. Applications Angiogenesis, tube and acini formation, colonization, neurite growth, tissue engineering, tissue regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation.... Non toxic and serum compatible 3D-FectIN™ is biodegradable. It does not affect cell growth and is compatible with serum and any culture medium. Collagen-Based Hydrogels Collagen-Derived Hydrogels Hyaluronic Acid Extracellular Matrix (ECM) Fibrin / Fibronectin Fibrinogen Laminin Matrigel TM (BD Biosciences) PEGylated hydrogels Collagen-based Scaffolds Collagen-derived Scaffolds Hyaluronic Acid Millicell TM (PTFE) (Millipore) Polycaprolactone Poly(Ethylene Glycol) Poly(lactic-co-glycolic acid) Poly(Styrene) Poly(Urethane) a a c b d a d b c Alkaline Phosphatase (AP) Secretion by HMEC-1 AP Concentration (pg/μL) hASC RAW COS-7 NIH-3T3 AP Concentration (pg/μL) Reagent 1 Reagent 2 Reagent 3 3D-FectIN High expression of secreted alkaline phosphatase c d AP concentration (pg/μL)
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Transfection reagents for gels and scaffolds supports
Transcript of OZ Biosciences 3D TRANSFECTION
ApplicationsTissue engineering, tissue regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation, colonization, neurite growth....
Highly efficient on cell lines & primary cells3D-Fect™ has been tested on a wide range of cells and achieves high transfection efficiencies. It is suitable for all culture conditions and does not affect matrice colonization and cell growth.
3D-Fect™ is a novel reagent, based on an innovative technology, specifically developed to directly transfect cells cultured in 3D scaffold. 3D matrices not only add a third dimension to cells' environment, they also allow creating significant differences in cellular characteristics and behavior. In this way, scaffold-based 3D matrices combined with 3D-Fect/DNA complexes are colonized by cells to be transfected in a more natural environment.
3D-Fect™ a new outlook for your cells !
Fig. 3: HMEC-1 transfected with 2, 3 or 4µg of DNA in a collagen-derived 3D-Scaffold.
l Highly efficient on cell lines and primary cells
l Specific for 3D scaffolds (3D-FectTM) or 3D hydrogels (3D-FectINTM)
l All type of nucleic acids: plasmid DNA, siRNA, shRNA...
l Applicable to a broad range of cells
3D-Fect™ & 3D-FectIN™ features
l Completely biodegradable
l Serum compatible
l Long term protein expression
l Simple, ready-to-use & rapid
Please visit www.ozbiosciences.com for more results
3D-FectIN™ a novel perspective for your cells !
Suitable for all nucleic acidsThis reagent is applicable for DNA, shRNA and siRNA transfection. The protocol is simple and rapid.
Fig. 2: Cells transfected on various 3D-scaffolds.a. HEK-293T - b. HeLa - c. MEF - d. Neural Stem Cells
Fig. 1: Cells transfected in a collagen-based 3D-Scaffold.
Alkaline Phosphatase (AP) Secretion
Fig. 6: NIH-3T3 transfected in a collagen-derived hydrogel with 3D-FectIN and others reagents.
Transfection Efficiency Comparison in NIH-3T3 cells
Fig. 4: Cells transfected in hydrogels.a. Raw 264.7 - b. HMEC-1 - c. HEK-293T - d. Neural Stem
Fig. 5: Cells transfected in a collagen-based hydrogel with 3D-FectIN.
Gentle to cells3D-Fect™ is biodegradable and allows high cell viability. It is serum compatible, thus no medium change is required.
Long term protein expression3D-Fect™ allows 3D trangene expression studies in in vivo like conditions over a long time period.
Specific for 3D-ScaffoldsExamples of 3D-Scaffolds successfully tested:
Outperforms competitorsHigher efficiency in term of number of transfected cells and transgene expression than other commercially available reagents.
Please visit www.ozbiosciences.com for more results
Versatile and universalIt is adapted to all culture conditions and suitable for all nucleic acids (DNA, shRNA, siRNA...). 3D-FectIN™ procedure is easy and straightforward.
Great efficiency on a variety of cells3D-FectIN™ is highly efficient and suitable for any cells: immortalized and primary. Transgene expression is high and long-lasting.
Outperforms competitors3D-FectIN™ achieves superior efficiency than other reagents
Specific for any hydrogels supporting 3D cultureExamples of 3D-Hydrogels successfully tested:
3D-FectIN™ is a new transfection technology specially engineered to directly transfect cells cultured in 3D hydrogel. 3D-FectIN™ adds a third dimension to cell culture!3D matrices allow cells to grow in micro-environment that more closely mimics the 3D environment encountered by cells in vivo. Thus, hydrogel-based 3D matrices combined with 3D-FectIN/DNA complexes allow cells to be directly transfected in more natural surroundings.
ApplicationsAngiogenesis, tube and acini formation, colonization, neurite growth, tissue engineering, tissue regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation....
Non toxic and serum compatible3D-FectIN™ is biodegradable. It does not affect cell growth and is compatible with serum and any culture medium.
Collagen-Based HydrogelsCollagen-Derived HydrogelsHyaluronic AcidExtracellular Matrix (ECM)Fibrin / FibronectinFibrinogenLamininMatrigel TM (BD Biosciences)PEGylated hydrogels
Collagen-based ScaffoldsCollagen-derived ScaffoldsHyaluronic AcidMillicellTM (PTFE) (Millipore)PolycaprolactonePoly(Ethylene Glycol)Poly(lactic-co-glycolic acid)Poly(Styrene)Poly(Urethane)
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ac
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Alkaline Phosphatase (AP) Secretion by HMEC-1
AP
Con
cent
ratio
n (p
g/µL
)
hASC RAW COS-7 NIH-3T3AP
Con
cent
ratio
n (p
g/µL
)
Reagent 1 Reagent 2 Reagent 3 3D-FectIN
High expression of secreted alkaline phosphatase
c
d
AP
conc
entr
atio
n (p
g/µL
)
