HEALTHY PEOPLE HEALTHY ENVIRONMENT

September 2006 Oxitec confidential

71 Milton Park, Abingdon, Oxford, OX14 4RX, UKTelephone +44 1235 832393 www.oxitec.com in Latin America: joaquim.leao@oxitec.com +55 21 9961 1960

Market Opportunity: Dengue Fever

� Global pandemic� More than 100 million cases

annually (WHO, CDC)� Asia and Latin America � Globalisation and global

warming drive spread

� Severity increasing� Increase in Dengue

Haemorraghic fever, a lethal complication

� No specific medication or vaccine

Oxitec Limited

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Dengue Fever

� Transmitted by Aedes aegypti mosquitoes

� Urban dwellers

� Also spreads yellow fever and chikungunya

The Dengue mosquito Aedes aegypti

� Over $1.0 billion worldwide spent on controls – largely ineffective� Insecticides� Breeding site sanitation

� New tools required

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Thermal insecticide fogging

Breeding site sanitation

Oxitec

� Environment-friendly technology to control insect pests:

� Public health – disease vectors

� Agriculture

� Global leaders in a new field Asian tiger mosquito� Global leaders in a new field

� Molecular biology and insect genetics

� Intellectual property

� Regulatory affairs

� Relationship management

Oxitec Limited

Asian tiger mosquito

Injecting DNA into mosquitoeggs

Oxitec

� Founded in 2002

� Building on innovative research by Dr. Luke Alphey at the University of Oxford

of Oxford

� 30 employees

� HQ in Oxford, UK

� Privately held with UK and US financing

Oxitec’s RIDL® Technology Solution

� Genetic enhancement of Sterile Insect Technique (SIT)

� SIT

� Rear millions of insects

� Sterilise with irradiation

New world screwworm eradicated from USA and Central America with SIT

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� Sterilise with irradiation

� Release over wide area

� Sterile males mate with wild females: progeny don’t survive

� Pest population declines

� Clean technology : Species-specific

� Used for over 50 years� But constrained by existing technology

Mediterranean Fruit Fly (USDA photo)

RIDL® Benefits

� RIDL insects are sterile in the absence of a diet supplement

� Available in lab and rearing facility

� Not available in nature

� Sterilization – no more irradiation

� Fitter, more competitive sterile insects improve cost-effectiveness

� Applicable to insects where irradiation is too damaging

� Fail-safe bio-containment

� Enhanced biosafety; reared insects are sterile outside the facility

� Sexing - release only males

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Irradiators at a mass-rearing facility

Mating Mediterranean Fruit Flies

Benefits: Improved monitoring

� Population monitoring essential for insect control

� Oxitec strains contain heritable, fluorescent markers� Improvement over fluorescent � Improvement over fluorescent

dusts and food dyes

� Field monitoring devices in development

Oxitec Limited

Pink Bollworm Pupae with red and green markers

Mediterranean Fruit Fly showing fluorescence at all life stages

Dengue: A Genetic Solution

� Bill and Melinda Gates Grand Challenges for Global Health award

� US/Mexico focused consortium

� Large scale field cages being constructed in Mexico

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constructed in Mexico

� Oxitec has RIDL strains of Aedes aegypti validated by external labs

� Product development in Malaysia

� Rolling out model worldwide

� Brazil, India, Other

Dengue fever mosquito, Aedes aegypti

Dengue product: Oxitec progress in Malaysia

� Agreement with Ministry of Health, Institute for Medical Research

� Excellent trial results from purpose built quarantine field-house

� Island identified for open field release and baseline data collection

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release and baseline data collection

� Regulators reviewing permit application for open release trial

� Negotiating production and distribution venture with local company

Purpose built quarantine field-house

Trial planning meeting on the island

Agriculture Opportunity: Insect Control

� Increasing global demand for pest-free, pesticide-free fresh produce

� Fruits, nuts and vegetables

� Quarantine and trade restrictions

� $1.0 billion accessible new market Cotton Pink Bollworm� $1.0 billion accessible new market

� $2.5 billion insecticide market in fresh produce crops - 40% accessible

� Replacement Sterile Insect Technique (SIT) market of $0.2 billion� Growth and profitability constrained by

existing technology

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Cotton Pink Bollworm

Codling moth in apple

Agricultural Progress: Pink Bollworm

� Cotton pest in USA

� SIT program since 1970s

� Oxitec ProgressCollaboration with USDA since � Collaboration with USDA since 2002

� Two years open field release

� Pilot in CA planned 2008

� Program conversion planned 2009

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Cage trials Arizona

DsRed marker in dead adult

Mediterranean Fruit Fly

� Pest of global economic importance� Quarantine pest prevents exports

� SIT used worldwide ~20 programs

� Oxitec Progress� RIDL strains tested by IAEA/FAO for

mass-rearing parametersMediterranean fruit fly

Oxitec Limited

mass-rearing parameters

� RIDL strains in testing with USDA in Guatemala

� Collaboration with USDA� Federal Business Opportunities

announcement of sole source contract

� Drive regulatory framework

Mediterranean fruit fly

USDA El Pino rearing facility Guatemala – rears 2 billion flies per week for US, Mexico, Guatemala

Regulatory and Public Acceptance

� Regulatory Framework

� The USDA is conducting an Environmental Impact Statement (EIS) on the use of autocidal technology in fruit flies and PBW

� North American Plant Protection Organization (NAPPO) standards issued in 2007

� Confined trials in Malaysia, USA, Guatemala, UK and Austria

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� Two years of open field testing in USA

� Public Acceptance

� PR strategy to focus on public health benefits

� Press attention positive so far

� Consultation with Gates and other ethics groups

Summary

� Oxitec has developed RIDL ® technology that can provide environmentally-friendly control of insect pests

� Oxitec’s technology will provide control of the mosquito that spreads dengue fever, an increasing global menace

Oxitec Limited

that spreads dengue fever, an increasing global menace

� Oxitec has established collaborations with the governments of USA and Malaysia

� Oxitec seeks institutional and commercial partners to develop and commercialise its technology worldwide