OUT
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OUT IN rClaudin-4 136-209 C N OUT IN rClaudin-4 108-209 C N OUT IN N C rClaudin-2 136-230 OUT IN N C rClaudin-2 108-230 Claudin-2 OUT IN N C C OUT IN N Claudin-4 A Claudin-4 chimera second extracellular loop claudin- 4/E2 OUT I N Claudin-4 Claudin-2 OUT I N OUT I N Claudin-2 chimera second extracellular loop claudin- 2/E4 OUT I N N N N N C C C C B
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OUT. OUT. OUT. OUT. IN. IN. N. N. C. C. rClaudin-2 136-230. rClaudin-4 136-209. IN. IN. N. N. C. C. Claudin-4. Claudin-2. OUT. OUT. OUT. OUT. IN. IN. N. N. C. C. rClaudin-2 108-230. rClaudin-4 108-209. IN. IN. N. N. C. C. - PowerPoint PPT Presentation
Transcript of OUT
OUT
IN
rClaudin-4136-209
CN
OUT
IN
rClaudin-4108-209 C
N
OUT
INN C
rClaudin-2136-230
OUT
INN C
rClaudin-2108-230
Claudin-2
OUT
IN NCC
OUT
INN
Claudin-4
A
Claudin-4 chimera second
extracellular loop claudin-4/E2
OUT
IN
Claudin-4 Claudin-2
OUT
IN
OUT
IN
Claudin-2 chimera second
extracellular loop claudin-2/E4
OUT
IN NN
NN
CC
CC
B
OUT
Claudin-4N149D N
C
OUT
IN NC
Claudin-1D150N
N
D
D
N
Claudin-2S149N
OUT
IN N
OUT
N
Claudin-4N149S
C C
S
N
N
S
C
Supplemental Fig. 1. Diagrams of claudins, chimeric claudins, claudin C-terminal fragments, and claudin mutants used in this study. (A) Predicted structure of the claudin components of recombinant claudin species used in Fig. 2 experiments. (B) Predicted structure of claudin chimera constructs used in Fig. 2 and 3, noting the change in the ECL-2 of Claudin-2/E4 and Claudin-4/E2. (C) Representation of claudin ECL-2 point mutations, with amino acid change indicated, that were used as soluble rClaudins in Fig. 5 experiments or were stably expressed by Rat1-R12 fibroblast transfectants in Fig. 6 studies.
