Other Specimens
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Tissue Specimens Tissue samples, including biopsies, surgical or necropsy specimens, collected for possible detection of parasites , should be submitted to the hospital path ologist. Specimens may be forwarded to the Division of Parasitic Diseases at CDC if further identification is necessary. Specimens may be submitted in several forms, including sectioned and stained slides, tissue blocks, or preserved tissues from cases that have a high suspicion of containing parasite material. In most inst ances, routinely stain ed hematoxylin and eosin sections are s uitable for examination for parasitic agents, and many times, an accurate identification of the parasite can be es tablished in tissue sections. Occasionally, additional section s are required to further the diagnosis. Ship samples accord ing to shipping gu idelines and requirements (see shipping guidelines). Care should be taken to pack glas s slides secur ely, as they can be damaged in shipment if not packed in a crush proof container. Tissue Specimens for free-living amebae (FLAs) Tissue specimens, including biopsy, surgical or necropsy specimens, may be collected for the detection of free-living amebae (Naegleria, Balamuthia, and Acanthamoeba ). The desired specimens include: • tissue slides stained with hematoxylin and eosin (H&E). • unstained slides (for indirect Immunofluorescenc e, or IIF). • unfixed brain tissue or CSF for PCR. • unfixed corneal scrapings (for Acanthamoeba ). • paraffin-embedded tissue block. Ship samples according to shipping guidelines and requirements (see shipping guidelines). Unfixed specimens f or culture sho uld be sent at ambient temperature by overnight priority mail. For PCR, sterile unfix ed specimens or specimen s in 70-90% ethanol should be sent by overnight priority mail on ice packs. Care should be taken to pack glass slides securely, as they can be damaged in shipment if not packed in a crush-proof container. Specimen submissio n forms for free-living amebae testin g can be requested by emailing [email protected].
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