Osseointegration of Dental Implants

OSSEOINTEGRATION

RAKESH CHANDRAN

‘a direct structural and functional connection between ordered, living bone and the surface of a load-bearing implant’

Osseointegration definition

Bosshardt et al 2017. Osseointegration of titanium, titanium alloy and zirconia dental implants. Perio 2000

IMPLANTMacroscopic structureMicroscopic structure

BONECellular level

osteoprogenitor cellsosteoblasts and lining cells osteocytesosteoclasts

Molecular levelmineralshormonesgrowth factorscytokinesextracellular matrix proteins

IMPLANT DESIGN AND SURFACE CHARACTERISTICS

Different types of implant designs and surface characteristics

Barfeie et al 2015. Implant surface characteristics and their effect on osseointegration. BDJ

Surface chemistry (inorganic or organic)Surface energy/charge and wettabilitySurface topography (micro and nano-topography)

Implant surface modification

Feller L et al 2015. Cellular responses evoked by different surface characteristics. Biomed Res Int

• exposure of the implant surface to an electrolytic environment and to ionic exchange can lead to adsorption and conformation of proteins which play a part in platelet adhesion

• electrochemical deposition, ion implantation, anodisation, plasma based fluorine ion application are examples of chemical methods of surface modification

• anodisation leads to a substantial thickness of the titanium oxide layer which may increase osseointegration

Surface chemistry


• defined as the excess energy at the surface of a material compared to the bulk• quantifies the disruption of intermolecular bonds that occur when a surface is

created• this extra energy provides the driving force for the adhesion to the surrounding

tissues. • so an active implant surface provides the required conditions for starting the

desired interaction with the cellular environment.• a negatively charged surface promotes better osseointegration

• surface energy of an implant can be modified by oxidisation, chemical and topographical modification and by plasma treatment.

Surface energy/charge


• largely dependent on surface energy• describes the ability of a liquid to maintain contact with a solid surface• determined by a balance between adhesive and cohesive forces• adhesive forces between a liquid and a solid cause a liquid drop to spread across

the surface • cohesive forces within the liquid cause the drop to ball up • calculated from the contact angle that is formed between the drop and the

surface.

Surface wettability


Examples of additive processesHydroxyapatite (HA) and other calcium phosphate coatings.Titanium plasma-sprayed (TPS) surfaces.Ion deposition.

Examples of subtractive processesElectropolishingMechanical polishingBlastingEtchingOxidation

Techniques to alter surface topography

Wennerberg and Albrektsson 2009. Effects of titanium surface topography on bone integration. Clin Oral Impl Res

Classification of surface roughness of implant surfaces

Albrektsson and Wennerberg 2004. Oral implant surfaces. Int J Prosthodont

Different types of implant designs and surface characteristics

Barfeie et al 2015. Implant surface characteristics and their effect on osseointegration. BDJBosshardt et al 2017. Osseointegration of titanium, titanium alloy and zirconia dental implants. Perio 2000

• moderately rough implant surfaces are believed to deliver better osseointegration compared with smooth surfaces however, results from different studies vary.

• currently, pure titanium is the ideal material for implants. • ceramics have the potential to become the next generation of dental implants but

currently there is not sufficient scientific evidence for routine use of ceramic implants.

• more standardised high quality prospective studies are required

Implant surface characteristics influence cellular responses such as cell adhesion, proliferation, differentiation and migration, thus affecting osseointegration


IMPLANTMacroscopic structureMicroscopic structure

BONECellular level

osteoprogenitor cellsosteoblasts and lining cells osteocytesosteoclasts

Molecular levelmineralshormonesgrowth factorscytokinesextracellular matrix proteins

Nanci A 2013. Ten Cate’s Oral Histology. 8th Ed. Mosby

Origin of bone cellsosteoblasts

MAJOR SIGNALING PATHWAYS• Wnt pathway (been shown to control the differentiation of both osteoblasts and

osteoclasts)• notch signaling (cell fate division and homeostatic maintenance, the notch

pathway is believed to be important in osteogenesis because of notch1–BMP-2 interactions that promote osteogenic differentiation)

• hedgehog proteins (plays a role in many embryonic processes and also is involved with the maintenance of stem cells in adults)

• TGF-β/BMP superfamily• fibroblast growth factors

TRANSCRIPTION PATHWAYS• Runt-related transcription factor 2/core-binding factor alpha 1 (Runx2/cbfα-1) • osterix

Mesenchymal stem cells differentiate into mature osteoblasts

Seitz et al 2013. Repair and Grafting of Bone. Plastic surgery

Receptor signaling typically leads to the formation or modification of biochemical intermediates and/ or activation of enzymes, and ultimately to the generation of active transcription factors that enter the nucleus and alter gene expression. Gene expression is the process in which information from a gene is used by the cell to produce a functional product, typically a protein.

DNA replication and RNA transcription and translation. Khan academy. www.khanacademy.org. Assessed 25 Feb 2017

Response to a signal. Khan academy. www.khanacademy.org. Assessed 25 Feb 2017

The mRNA leaves the nucleus and enters the cytosol. There, it directs synthesis of a protein, indicating which amino acids should be added to the chain. This step is called translation

• Type 1 collagen• Cell adhesion proteins (osteopontin, fibronectin, thrombospondin)• Calcium-binding proteins (osteonectin, bone sialoprotein)• Proteins involved in mineralization (osteocalcin)• Enzymes (collagenase, alkaline phosphatase)• Growth factors (IGF-1, TGF-β, PDGF)• Cytokines (IL-1, IL-6, RANKL)

Proteins concentrated from serum• β2-microglobulin• Albumin

Proteins of the bone matrix

Seitz et al 2013. Repair and Grafting of Bone. Plastic surgery


Osteoblasts, lining cells, osteocytes and osteoclasts

TRAP, tartrate-resistant acid phosphatase


Origin of bone cells- osteoclasts

Risteli et al 2012 Bone and mineral metabolism

RANKL stimulates osteoclast activation by inducing secretion of protons and lytic enzymes into a sealed resorption vacuole formed between the basal surface of the osteoclast and the bone surface. Acidification of the vacuole leads to activation of tartrate- resistant acid phosphatase (TRACP) and cathepsin K (Cat K)


Osteoblasts, lining cells, osteocytes and osteoclasts

OSSEOINTEGRATION

Phase 1: hemostasisPhase 2: inflammatory phasePhase 3: proliferativePhase 4: remodelling

Series of events leading to osseointegration

Terheyden et al 2012. Osseointegration – communication of cells. Clin Oral Impl Res.

Phase 1: hemostasis (minutes to hours)Phase 2: inflammatory phase (after 10 minutes to a few days)Phase 3: proliferative (few days to a few weeks)Phase 4: remodelling (few days to a few years)

Series of events leading to osseointegration- duration


Phase 1: hemostasis (from trauma to the degranulation of platelets)Phase 2: inflammatory phase (from degranulation of platelets to attachment of fibroblasts)Phase 3: proliferative (from attachment of fibroblasts to formation of woven bone)Phase 4: remodelling (removal of woven bone and formation of lamellar bone)

Series of events leading to osseointegration- initiating and concluding events


• trauma to bone• growth and differentiation factors are unmasked and liberated from their heparin

binding domains by heparin hydrolases from blood platelets• platelets release

• platelet derived growth factor (PDGF)• transforming growth factor (TGF) β1 and β2• insulin like growth factor (IGF)• vascular endothelial growth factor (VEGF)• basic fibroblast growth factor (bFGF)• coagulative and vasoactive agents

• platelets initiate polymerization of fibrinogen to form an extracellular matrix by thrombin (extrinsic system) and the intrinsic clotting cascade (Hageman Factor)

• platelets aggregate and form a white thrombus closing the vascular leak.

Phase 1- hemostasis


• implant surface interacts with water molecules and ions- this can change the charge pattern of the surface

• this is followed by plasma proteins present at high concentrations like albumin, globulins or fibrin

• these will slowly be replaced by proteins with a lower concentration, but a higher affinity for the surface such as vitronectin or fibronectin

Phase 1- hemostasis


• platelets bind to vitronectin. This activates platelets, converting the major platelet integrin αVβ₃ from a resting state to an active conformation.

• platelets also bind to collagen with glycoprotein receptors• surface bound fibrin on the metal surface of the implant can bind platelets over

the glycoprotein receptor to the titanium implant surface.• this binding results in activation and degranulation of the platelets• the release of cytokines from degranulating platelets is the beginning of the

inflammatory phase.

Phase 1- hemostasis


Cell to cell communication- Osseointegration. Quintessence Publishing

• degranulated platelets release• TGF-β• PDGF• bFGF• bradykinin (increases the vascular permeability) • histamine (increases blood flow, decreases the blood stream velocity and

induces hyperaemia).• innate host defence systems are activated

• molecular (e.g. complement system)• cellular elements (PML/ PMN and macrophages)

Phase 2: Inflammatory phase


• diapedesis is initiated by loose adhesion of lectins in the inner lining of blood vessels.

• mediated by adhesion of L-selectin on the leucocyte with E-selectin on the endothelial side

• later stronger adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1), ICAM-2 and the vascular cell adhesion molecule-1 (VCAM-1) catch the leukocyte out of the blood stream, binding to integrins on the leucocyte

• PMN produce elastase and collagenase which helps them in digesting the basal lamina of the blood vessel and pass beyond the basal lamina.

• endothelial cells open a small gap, and the leukocyte migrates in amoeboid fashion through the gap.

• PMLs kill bacteria through reactive radicals (oxygen species and hydroxygroups, chlorine radicals and hypochlorites) which are also toxic for the host cells and the healthy tissue surrounding the wound.

Phase 2: Inflammatory phase- PML


• first published by a German pathologist Karl Donath in 1992• although neutrophils are recruited on the basis of a pure wound healing

phenomena, macrophages are only recruited if a biomaterial is present• macrophages are found frequently on the surface of titanium oral implants and

justify the concept of osseointegration being a foreign body reaction, because oral implants are in themselves foreign bodies.

Concept of foreign body equilibrium

Trindade et al 2015. Current Concepts for the Biological Basis of Dental Implants. Oral Maxillofac Surg Clin North Am

a) osseointegration is the result of a foreign body reaction that, with the right intensity in the inflammatory response, will balance itself out and allow for bone to grow on the implant surface

b) similar to soft tissue implants, which end up encapsulated in poorly enervated and vascularized fibrous tissue, dental implants also become surrounded by condensed bone that is very poor in vascularization and enervation, the typical result of a foreign body reaction that has reached equilibrium

c) the ongrown bone may be seen as a manner of shielding off the foreign entity from the tissues, that is, as a protective mechanism.

Concept of foreign body equilibrium

Albrektsson et al 2014. Is marginal bone loss around oral implants the result of a provoked foreign body reaction?

• PML are relatively short-lived and are replaced by lymphocytes and macrophages.• macrophages remove tissue debris• macrophages also secrete TIMPs (tissue inhibitors of metalloproteinases) which

antagonize the digesting enzymes of PMN and therefore protect the extracellular matrix proteins like proteoglycans. These in turn can protect growth factors which are stored in the extracellular matrix.

• macrophages secrete angiogenic and fibrogenic growth factors.• high concentration of fibronectin allows attachment of fibroblasts• cells can hereupon crawl into the wound• this is the beginning of the proliferative phase

Phase 2: Inflammatory phase- macrophages


FORMATION OF NEW ECM• this newly formed tissue is called granulation tissue.• macrophages release FGF which leads to migration of fibroblasts from the

surrounding healthy tissue into the blood clot. • metalloproteinases degrade the blood derived fibrin in the clot und uncover

integrin binding sites in the fragments. • to replace the degraded provisional clot matrix, they produce insoluble cellular

fibronectin and other insoluble proteins of the extracellular matrix like collagens, vitronectin, decorin and other proteoglycans.

Phase 3: Proliferative phase- the formation of new ECM, angiogenesis and woven bone


Feller et al 2015. Cellular Responses Evoked by Different Surface Characteristics of Intraosseous Titanium Implants.

Phase 3- focal adhesion

ANGIOGENESIS• in parallel, angiogenesis is stimulated by hypoxia. • hypoxia attracts macrophages, which are able to survive under hypoxic conditions

by adjusting their metabolism to an oxygen independent generation of ATP. In macrophages, vascular endothelial growth factor (VEGF) expression is stimulated by an intracellular transcription factor called hypoxia inducible factor (HIF-1).

• In response to VEGF, pericytes detach from the outer walls of the vessel. These cells use matrix metalloproteinases to digest the basal lamina around the vessels.

• The pericytes give rise to the new endothelial progenitor cells, which migrate to the place of low oxygen tension where they are chemotactically attracted by the chemokine stromal cell derived factor (SDF-1) which is produced by cells in the wound. This process is called homing of endothelial cells.

• The cells proliferate to form condensed groups and they arrange themselves to form tubes and these tubes are connected to an existing blood vessel.

• A new vascular loop is created and blood can flow through.

Phase 3: Proliferative phase- the formation of new ECM, angiogenesis


FORMATION OF WOVEN BONE• new bone formation begins with the secretion of a collagen matrix by osteoblasts• depending on the process of ossification (endochondral or intramembraneous),

this can be collagen type II or type III, which is ultimately replaced by collagen type I.

• bone formation within the alveolar process is a process of intramembranous ossification, starting by the secretion of collagen type III.

• this matrix is subsequently mineralized by hydroxyapatite. • then nanometer-sized, uniaxially oriented hydroxyapatite crystal plates are

formed within the collagen fibres.• removal of the woven bone by osteoclasts is the beginning of remodelling and

thus the fourth and last phase.

Phase 3: Proliferative phase


• osteoclasts appear in the wound after a few days.• primary bone implant contacts are removed. • lamellar bone formed after remodelling• in contrast to woven bone which is oriented parallel to the titanium surface in the

grooves of the threads, lamellar bone attaches rather to the tips of the macrothreads.

Phase 4: remodelling phase


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Osseointegration of Dental Implants

Health & Medicine

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