Osmosis in RBC
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Transcript of Osmosis in RBC
OSMOSIS IN RED BLOOD CELLKnights of the Long Table
Introduction
Osmosis is a special type of diffusion.
Tonicity is the ability of a solution to induce a net flow of water through a selectively permeable membrane.
Objective
The experiment aims to show what would happen to red blood cells when subjected to hypotonic, hypertonic and isotonic solutions
Materials and Methods
Materials and Methods
Materials and Methods
NaCl Solution
0.07M 0.15M 0.30M
Materials and Methods
Every 10
minutes
Results
Slide Initial cell size(mm)
Average cell size(mm)
A(0.07 M NaCl)
0.004,0.006,0.006 0.005
B(0.15 M NaCl)
0.105,0.105,0.105 0.105
C(0.30 M Na
0.14,0.105,0.105 0.117
Table 1. Initial Size of Red Blood Cells
Results
0 5 10 15 20 25 30 35 40 450
0.001
0.002
0.003
0.004
0.005
0.006
0.007
0.008
Figure 1. Size of Red Blood Cells on Slide A (0.07 M NaCl)
Results
0 5 10 15 20 25 30 35 40 450
0.02
0.04
0.06
0.08
0.1
0.12
Figure 2. Size of Red Blood Cells on Slide B (0.15M NaCl)
Results
0 5 10 15 20 25 30 35 40 450
0.02
0.04
0.06
0.08
0.1
0.12
0.14
Figure 3. Size of Red Blood Cells on Slide C (0.3M)
Results
Graph 1 showed no net increase or decrease in the cell size of Red Blood Cells treated with O.07 M NaCl.
Graph 2 showed no net increase or decrease in the cell size of Red blood cells treated with 0.15 M NaCl.
Graph 3 showed a net decrease in cell size of Red blood cells treated with 0.3 M NaCl
Discussion
Osmosis is a process by which water moves through a selectively permeable membrane. The net flow of water would be from a region of lower solute concentration to that of a higher solute concentration until both have an equal concentration on both sides of the selectively permeable membrane (Starr & Taggart, 1995). Hypotonic solution results in Hemolysis
Isotonic solution
Hypertonic solution results in Crenation
Discussion
Some of our experimental results differ with that of the theoretical results .
The measurement of the cell sizes were determined by getting the average of three random cells in the slide which can be a source of error because those three cells might not represent the majority of the cells.
Another error could be the uneven distribution of the NaCl throughout the slide which might not have reached other areas.