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Organizing committee
Chair Man Bock Gu School of Life Sciences and Biotechnology for Brain
Korea21 PLUS, Korea University, Korea
Co-Chair Tri-Nhan Nguyen Faculty of Biology - Biotechnology, University of Science,
Vietnam National University Ho Chi Minh City
Committee
members
In-Geol Choi School of Life Sciences and Biotechnology for Brain
Korea21 PLUS, Korea University, Korea
Sung Ok Han School of Life Sciences and Biotechnology for Brain
Korea21 PLUS Korea Universtiy, Korea University, Korea
Hyun Jin Park School of Life Sciences and Biotechnology for Brain
Korea21 PLUS, Korea University, Korea
Kwang Won Lee School of Life Sciences and Biotechnology for Brain
Korea21 PLUS, Korea University, Korea
Sung Joon Lee School of Life Sciences and Biotechnology for Brain
Korea21 PLUS, Korea University, Korea
Van-Thuan Nguyen School of Life Sciences and Biotechnology for Brain
Korea21 PLUS, Korea Universtiy, Korea
Van-Hieu Tran Faculty of Biology - Biotechnology, University of Science,
Vietnam National University Ho Chi Minh City
Duc-Hoang Nguyen Faculty of Biology - Biotechnology, University of Science,
Vietnam National University Ho Chi Minh City
Hai-Nhung Truong Faculty of Biology - Biotechnology, University of Science,
Vietnam National University Ho Chi Minh City
Bao-Ha Tran-Le Faculty of Biology - Biotechnology, University of Science
- VNUHCM
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Symposium Venue
Room F102, University of Science – VNUHCM, 227 Nguyen Van Cu St., Dist. 5, Ho Chi Minh City, Vietnam
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Program at a Glance
Plenary session
8.30 ~ 8.40 Introduction of KU/Department of Biotechnology for BK21 PLUS Prof. Man Bock Gu…………………………………………………………..………… 5
8.40 ~ 8.50 Introduction of HCMUS/ Faculty of Biology - Biotechnology Dr. Nguyen Tri Nhan…………………………………………………………………....5
Session 1. Food Biotechnology and Therapeutics
Chairs: Associate Prof. Nguyen Duc Hoang, Prof. Man Bock Gu
8.50 ~ 9.05
Inorganic Arsenic Bioaccessibility/Bioavailability from Cooked Rice Using
In Vitro Digestion/Caco-2 Cell Model
Kwang Won Lee
Department of Biotechnology, School of Life Sciences and Biotechnology, Korea
University, Seoul, Republic of Korea………………………………………………… 7
9.05 ~ 9.20
Nano delivery system for practical food system
Hyun Jin Park
Department of Biotechnology, School of Life Sciences and Biotechnology, Korea
University, Seoul, Republic of Korea ……………………………………………….. 8
9.20 ~ 9.35
Mechanism of dietary anthocyanins on the regulation of energy metabolism
Sung-Joon Lee
Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea ………………………………………………. 10
9.35 ~ 9.50
Mesenchymal stem cell: a potential therapy for liver cirrhosis treatment
Nhung Hai Truong
Faculty of Biology - Biotechnology, HCM University of Science………………….12
9.50 ~ 10.05
Nano chitosan for protein delivery
Hieu Tran-Van
Faculty of Biology - Biotechnology, HCM University of Science………………….13
10.05 ~ 10.15 Coffee break
Session 2. Biosensor and Bioengineering
Chairs: Dr. Nguyen Tri Nhan, Prof. In-Geol Choi
10.15 ~ 10.30
C1/3/5/6 Biorefinery by using Designer Nanoscale Enzyme Complexes
Sung Ok Han
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Department of Biotechnology, School of Life Sciences and Biotechnology, Korea
University, Seoul, Republic of Korea………………………………………………. 15
10.30 ~ 10.45
Aptamer-based Nanobiosensors and Biomolecular Technology Research at Korea University
Man Bock Gu
Department of Biotechnology, School of Life Sciences and Biotechnology, Korea
University, Seoul, Republic of Korea ………………………………………………. 17
10.45 ~ 11.00
Computational & Synthetic Biology Laboratory - the Future of G3 Technology In-Geol Choi
Department of Biotechnology, School of Life Sciences and Biotechnology, Korea
University, Seoul, Republic of Korea………………………………………………. 18
11.00 ~ 11.15
Acellular adipose matrix: collection, evaluation, potential applications
Tran Le Bao Ha
University of Science, Vietnam National University, Vietnam ……………………19
11.15 ~ 11.30 Non-inducible and IPTG-inducible expression vectors for protein production based on Pgrac promoters for Bacillus subtilis
Nguyen Duc Hoang
Faculty of Biology - Biotechnology, VNUHCM-University of Science, Hochiminh City, Vietnam…………………………………………………………………….…….20
11.30 ~ 11.40 Photo & Coffee break
11.40 ~12.15
Q&A for students & Interview-Recruiting student session
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Plenary session
8.30 ~ 8.40
Introduction of KU/Department of Biotechnology for BK21 PLUS
Prof. Man Bock Gu
8.40 ~ 8.50
Introduction of HCMUS/ Faculty of Biology - Biotechnology
Dr. Nguyen Tri Nhan
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Session 1. Food Biotechnology and Therapeutics
Chairs: Dr. Nguyen Tri Nhan (Faculty of Biology - Biotechnology, HCM University of Science)
Prof. Man Bock Gu (Department of Biotechnology, School of Life Sciences and Biotechnology,
Korea University, Seoul, Republic of Korea)
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Abstract Session 1. Food Biotechnology and Therapeutics
Inorganic Arsenic Bioaccessibility/Bioavailability from Cooked Rice
Using In Vitro Digestion/Caco-2 Cell Model
Kwang Won Lee
Department of Biotechnology, School of Life Sciences and Biotechnology,
Korea University, Seoul, Republic of Korea
E-mail: [email protected]
Rice is one of the major sources for exposing inorganic arsenic (As) in food. Using in
vitro digestion/Caco-2 cell model, the bioaccessibility and bioavailability of As from
cooked rice were studied. Inorganic arsenic, especially As (III), was predominant and
bioaccessible species in cooked rice. The percentage of bioaccessibility of total As in
white rice (75%) was slightly higher (p=0.061) than brown rice (66%). However, there was
no different of bioaccessibility of iAs between white rice (95%) and brown rice (96%). In
Caco-2 cell monolayer, As uptake considering sum of retention and transport was
determined to be 16 to 38 %. As bioavailabilities in cooked white and brown rice were
31 % and 21 %, respectively indicating that the bioavailability of As from cooked rice can
be affected by matrix environment. The in vitro digestion/Caco-2 cell model may provide
an important information on risk assessment of As in rice.
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Abstract Session 1. Food Biotechnology and Therapeutics
Nano delivery system for practical food system
Hyun Jin Park
Department of Biotechnology, School of Life Sciences and Biotechnology,
Korea University, Seoul, Republic of Korea
E-mail: [email protected]
Nutraceuticals/functional foods can prevent various diseases and enhance physiological
performances. However, there are some limitations due to their poor solubility and very
low bioavailability. It is necessary to find out the optimum nanoformulations for different
nutraceuticals to improve their water solubility, absorption rate, and bioavailability. The
aim of this study is to provide a potential of nano delivery systems such as nanostructured
lipid carriers(NLCs), nanoemulsions(NEs), and nanoliposomes(NLs) which could improve
the encapsulation efficiency and bioavailability.
Chitosan coated NLCs(CH-NLCs) were developed for iron fortification by melt dispersion
method. Physicochemical properties of NLCs and CH-NLCs were investigated by
different concentration of emulsifier and chitosan to determine the optimum condition of
NLCs and CH-NLCs. In case of capsaicin NEs, the solubility of capsaicin in different oils
was measured to find out optimum NE formulations. NEs were produced on various ratios
of oil, surfactant, and water. Skin permeation studies were performed using a franz
diffusion cell method. Confocal laser scanning microscopy images were examined to
verify the penetration of the capsaicin NE. Chitosan coated curcumin nanoliposomes(CS-
Cur-NLs) were fabricated by ethanol injection method using cholesterol and L-α-
phosphatidylcholine (EPC) in ratio of 1 to 2. The mucoadhesive property of CS Cur-NLs
was measured by mucin adsorption study. The release rate of nano-carriers was tested
and their morphologies were characterized using transmission electron microscopy.
In conclusion, the encapsulation efficiency and in vitro release test showed that CH-
NLCs had a great potential for use of iron fortification in milk. In case of capsaicin NEs, it
was successfully developed by controlling the ratios of the surfactant mixtures, the oil
phase, and aqueous phase to enhanced skin permeation. For CS-Cur-NLs, the
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mucoadhesive property of CS-Cur-NLs was higher than that of non-coated Cur-NLs. CS-
Cur-NLs will show a potential for the prolonged adsorption in the gastrointestinal tract due
to higher mucoadhesion.
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Abstract Session 1. Food Biotechnology and Therapeutics
Mechanism of dietary anthocyanins on the regulation of energy
metabolism
Sung-Joon Lee
Department of Biotechnology, School of Life Sciences and Biotechnology,
Korea University, Seoul, Republic of Korea
E-mail: [email protected]
Cyanidin is a major dietary anthocyanidin in several plant-based foods including berries
and colored rice. It has been suggested that cyanidin has potent biological activities in
the regulation of cellular energy metabolism; however, molecular target(s) and the mode
of action of cyanidin have been elusive. The surface plasmon resonance and time-
resolved fluorescence resonance energy transfer analyses revealed that cyanidin and its
glycosides interacted with nuclear hormone receptorsk peroxisome proliferator-activated
receptors (PPARs) and liver X receptors, but with the highest affinity to PPARα. Cyanidin
and its glycoside significantly reduced triglyceride concentration in lipid-loaded HepG2
cells, via activation of the rate of fatty acid oxidation and suppression of the rate of fatty
acid synthesis in cultured hepatocytes. In high-fat-diet fed mice, oral administration of
cyanidin glycoside reduced plasma and hepatic triglyceride concentrations and improved
glucose and insulin tolerance, however, these metabolic activities of cyanidin glycoside
were abrogated in PPARα deficient mice. These suggest that PPARα may be a major
protein target for cyanidin and its glycoside in vivo. Several line of research showed that
cyanidin also activated AMP-activated kinase, which is a key regulator in cellular energy
metabolism and our recent results revealed that cyanidin glycoside activated AMP-
activated kinase not by direct allosteric mechanism but by indirect stimulation of upstream
kinase. Collectively, these results demonstrate that cyanidin may interact with more than
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one protein regulator, potentially with the highest affinity to PPARα. Activation of liver X
receptor and AMP-activated kinase as well as PPARα may and liver X receptor and
stimulate AMP-activate kinase may together contribute to the reduction of hepatic and
plasma lipid accumulation and improvement of insulin sensitivity.
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Abstract Session 1. Food Biotechnology and Therapeutics
Mesenchymal stem cell: a potential therapy for liver cirrhosis treatment
Nhung Hai Truong1,2*, Nam Hai Nguyen1, Trinh Van Le1, Huy Minh Le3, Nghia Huynh3,
DatQuoc Ngo3, Thanh Van Nguyen4, NgocKim Phan1,2, Phuc Van Pham1,2
1Laboratory of Stem Cell Research and Application, University of Science, VNU-HCM,
Vietnam
2Faculty of Biology and Biotechnology, University of Science, VNU-HCM, Vietnam
3University of Medicine and Pharmacy, HCM city, Vietnam
4Nguyen Tat Thanh University, Ho Chi Minh city, Vietnam
*Corresponding author email: [email protected]
Livercirrhosis is defined as the replacement of healthy liver tissues by fibrosis and regenerative
nodule formation. Cirrhosis ranks 14th in the world and 4th in Central Europe as cause of death.
Orthotopic liver transplantation (OLT) is the priority treatment for decompensated livercirrhosis.
Nonetheless, patients face many obstacles, such as the high costs, high risk from invasive
surgery, limitation of donor tissue, and lifelong immunosuppressive treatment. In recent years,
stem cell therapy has become a promising therapy for treatment. Some in vitro studies and in
vivo preclinical trials have demonstrated that mesenchymal stem cells (MSCs) may improve
livercirrhosis. However, application of MSCs in livercirrhosis treatment is still controversial
issues including MSC sources, routes of transplantation, “homing” into liver and hepatic
differentiation in vivo. To clarify any ambiguity some issues, we test the hypothesis that
autologous and allogenic mesenchymal stem cell sameliorate livercirrhosis in mice. The results
showed that MSC transplantation improved AST/ALT/bilirubin/albumin index after 7 days of
injection (p<0.05); significantly down-regulate gene expression of TGF-beta, procollagen and
nt5e (p<0.05). Both MSCs autologus and allogenic transplantation accelerated liver
regeneration capacity in mice after 21 days of transplantation. Considering safety, MSC
transfusion via a peripheral vein is a potential method for liver fibrosis treatment.
Keywords: Mesenchymal stem cell, livercirrhosis/fibrosis treatment, stem cell therapy, liver
disease, transplantation.
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Abstract Session 1. Food Biotechnology and Therapeutics
Nano chitosan for protein delivery
Hieu Tran-Van
Department of Molecular and Environmental Biotechnology, University of Science,
VNU-HCM
*E-mail: [email protected]
Fibroblast growth factor 2 (FGF-2) is a multi-functional protein regulating many biological
processes, especially those involved in wound healing. However, FGF-2 has short half-
life and is easily degradable in vivo, which limited its use for wound treatment. In this
study, we investigated a drug-delivery model for FGF-2 via incorporation with
biodegradable carboxylmethyl chitosan (CMCS), a substance classified as safe for use
in human by the Food and Drug Administration. We showed that CMCS nanoparticles
(NPs) could be synthesized by ionic gelation method using CaCl2 as a crosslinking
reagent with the CMCS:CaCl2 ratio of 1:0.8. Synthesized CMCS NPs were spherical with
an average diameter of 32.68±6.83 nm, and were non-toxic at concentrations up to 2.5
mg/ml. Incorporated CMCS:FGF-2 NPs had an average diameter of 34.83±5.89 nm and
FGF-2 incorporation efficiency was 95%. CMCS:FGF-2 NPs released 36.36% and 58.47%
of FGF-2 after 48 hours incubation in two different pH of 7.4 and 5.8, respectively. The
incorporation and release processes did not have a significant effect on FGF-2 activity.
Simultaneously, CMCS:FGF-2 NPs could protect FGF-2 from the degradation of trypsin
in vitro. Our results laid the groundwork for the manufacturing of protein incorporated
CMCS NPs for bio-applications.
Keywords: FGF-2, carboxylmethyl chitosan NPs, ionic gelation, drug release
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Session 2. Biosensor and Bioengineering
Chairs: Dr. Nguyen Tri Nhan (Faculty of Biology - Biotechnology, HCM University of Science)
Prof. In-Geol Choi (Department of Biotechnology, School of Life Sciences and Biotechnology,
Korea University, Seoul, Republic of Korea)
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Abstract Session 2. Biosensor and Bioengineering
C1/3/5/6 Biorefinery by using Designer Nanoscale Enzyme Complexes
Sung Ok Han
Department of Biotechnology, School of Life Sciences and Biotechnology,
Korea University, Seoul, Republic of Korea
E-mail: [email protected]
Cellulolytic anaerobic bacteria have evolved intricate multi-enzyme complexes with
scaffolding proteins known as cellulosomes. Cellulosomes are promising approach for
solving of the problems related to slow enzymatic degradation rates because complexed
system showed degradative potential. Cellulosome system have been applied to improve
the performance of immobilized enzymes and architectural scaffolds have applied to
useful strategies for protein engineering in several industrial and research fields. The
utilization of scaffolds for enzyme immobilization involves advanced bionanotechnology
applications in C1/5/6 biorefinery, which can be achieved by optimizing the function of
various enzymes. The designer enzymes and microbes are a key biological technology
that can be used for C5/C6 biorefinery. Prior to fermentative production of valuable
product from the C5/C6 based polysaccharides such as lignocellulosic and marine
biomasses by microbes, the polymeric substrates are hydrolyzed to release monomeric
sugars. The assembly of minicellulosomes by S. cerevisiae and Corynebacterium
glutamicum increased the activity against various lignocellulosic materials by
approximately 3-fold compared with control. Also, red algae-degrading complexes was
produced by Escherichia coli and increased the activity against the marine biomass
substrate by approximately 2-fold, compared with that for the corresponding enzymes
alone. Furthermore, cellulosome based enzyme complex is proposed for C1 biorefinery.
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C1 gas was successfully converted by functional complexes containing conversion
enzyme and gas binding protein with enhanced gas binding affinity. An enzyme complex
for biological conversion of C1 gas was anchored on the cell surface of industrial strains
and showed 3.3-fold increased conversion efficiency. The assembly of multi-functional
protein complexes showed efficient enzymatic processes by enhancing hydrolysis as well
as interactions between enzymes and their substrates. These results suggest that the
complexed system may be a promising strategy for C1/5/6 biorefinery as biological tools
for production of biochemicals such as bioplastics. Thus, the development of various
configurations of multi-functional protein complexes for use as tools in whole-cell
biocatalyst systems has drawn considerable attention as an attractive strategy for
bioprocess applications.
References
1. J. E. Hyeon, S. D. Jeon, S. O. Han, Biotechnol. Adv., 31(6), 936 (2013)
2. J. E. Hyeon, S. W. Kim, C. Park, S. O. Han, Chem. Comm., 51, 10202 (2015)
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Abstract Session 2. Biosensor and Bioengineering
Aptamer-based Nanobiosensors and Biomolecular Technology
Research at Korea University
Man Bock Gu
Department of Biotechnology, Korea University
*E-mail: [email protected]
Aptamers are single-stranded nucleic acids having molecular recognition properties
similar to antibodies, and isolated by in vitro selection and amplification process, SELEX.
This nucleic acid aptamer is well-known with its stable feature, quick and easy
developement, cost-effective production in vitro, and so on. To find a most effective
atpamer screening method has always been a goal, especially for obtaining a pair of
aptamers binding at different sites of a single target. This talk will start with how the
aptamers are innovatively screened, for the first time in the world, by using a new nano-
material, graphene, without the immobilization of targets. A few of successful examples
using immobilization-free screening of aptamers will be presented, including a few
aptamer duo cases, inevitable for being applied in a stripe-type platform, which could be
a reliable platform for commercialization eventually. In addition, some examples obtained
from the interactions among biomolecules, nanoparticles, or targets will be shown for
aptamers, bioluminescent bacteria, and enzymes with their successful implementation.
In addition, the benefits of using nano-sized materials for biosensing and other
biotechnological applications will be presented with scientifically proven clear examples
of different organic-inorganic hybrid forms of nanomaterials and biomolecules.
Keywords: Aptamers, Graphene-oxide, Enzyme stabilization, Bioluminescent Bacteria
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Abstract Session 2. Biosensor and Bioengineering
Computational & Synthetic Biology Laboratory - the Future of G3
Technology
In-Geol Choi
Department of Biotechnology, School of Life Sciences and Biotechnology,
Korea University, Seoul, Republic of Korea
E-mail: [email protected]
Biology is Technology. The Computational and Synthetic Biology Laboratory (CSBL)
aims to discover biological knowledge and make a full integration of theory and practice.
We construct metabolic pathways in genomes by design (e.g. iPNN - intelligent Pathway
Network Navigator) and learn how nature constructs ‘things’ by synthesis (e.g. PKSDS -
PolyKetide Synthetase Design Suite). CSBL gears undergraduate research programs,
the Korea_U_Seoul team for iGEM and supports DIYBio Movements in Korea. One of the
major achievements made by the CSBL is elucidation of the details of bacterial agarolytic
pathways. Agar, a recalcitrant polysaccharide, has a great potential as a renewable
biomass. We have sequenced genomes (DNAseq) and transcriptomes (RNAseq) of
several agarolytic microorganisms using next generation sequencing (NGS) techniques.
We have identified key enzymes (e.g. beta-agarases, agarooligosaccharide beta-
galactosidase - ABG, neoagarobiose hydrolase - NABH, anhydrogalactose
dehydrogenase -AHGD and anhydrogalactonate cycloisomerase - ACI, etc.) in the agar
metabolic pathway and determined atomic structures of key enzymes. The full
understanding molecular and cellular functions of these novel agarolytic enzymes will
provide the design principle of synthetic agar degradation pathways and eventually guide
the construction of synthetic microorganisms converting agar into valuable chemicals.
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Abstract Session 2. Biosensor and Bioengineering
Acellular adipose matrix: collection, evaluation,
potential applications
Tran Le Bao Ha1*, Nguyen Thi Ngoc My1, Nguyen Duc Thai1, Michele Zocchi2
1University of Science, Vietnam National University, Vietnam
2Turin Medical University, Italy
*E-mail: [email protected]
Our research interest is tissue engineering which includes fabrication of new biomaterials
as scaffolds for cells/stem cells into generation of functional tissues. Currently, we focus
on soft and hard tissue engineering as well as cancer tissue engineering. One of
concerned biomaterials is acellular adipose matrix (AAM) which is used not only for tissue
engineering as a natural scaffold, but also for fat grafting. Our group has started to
establish a reliable and efficient method for producing AAM. AAM is obtained from
liposuction adipose tissue and prepared by a decellularization process to eliminate all the
cellular components and reducing the DNA content to minimal levels. We also aim to
examine AAM as a scaffold for soft-tissue regeneration. In the near future, we aim to
produce AAM as ready-to-use, off the shelves and suitable materials for many clinical
applications.
Key words: tissue engineering, biomaterial, decellularization, scaffold, regeneration
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Abstract Session 2. Biosensor and Bioengineering
Non-inducible and IPTG-inducible expression vectors for protein
production based on Pgrac promoters for Bacillus subtilis
Trang Phan, Nguyen Duc Hoang*
Faculty of Biology - Biotechnology, VNUHCM-University of Science, Hochiminh City,
Vietnam; *E-mail: [email protected]
Bacillus subtilis possesses excellent properties to be considered as a host for protein
production. These properties include GRAS status and available large scale production.
Also, this endotoxin-free bacterium confers significant advantages to producing
recombinant protein products for animal and human uses. The development of these
products requires efficient optional expression systems for this bacterium. Here, we will
present a topic on the development of pHT expression vectors based on the promoter
Pgrac family to produce recombinant proteins in B. subtilis, which can use in an IPTG-
inducible or non-inducible manners. First, we introduced a new synthetic promoter Pgrac
derived from B. subtilis groESL promoter and E. coli lac operator, which can be induced
by addition of IPTG. This promoter is 50 times stronger than Pspac, the most popular
IPTG-inducible promoter used for B. subtilis. Second, by changing the core promoter
elements and the mRNA stabilizing elements to make an 84-promoter library, we showed
that the second generation of Pgrac promoters could enhance production levels more
than 30% of total cellular proteins in B. subtilis. Third, we generated potent expression
vectors containing a second generation promoter of Pgrac family, which could be used to
produce efficiently intracellular or extracellular recombinant proteins in B. subtilis. Finally,
we will update recent development of novel non-inducible vectors using the IPTG-Pgrac
promoter, which allowed production of recombinant proteins without the addition of IPTG.
The use of IPTG-inducible and non-inducible expression vectors harboring Pgrac
promoter will promise to bring enormous benefits to industrial applications.
Keywords: Pgrac, pHT vectors, Pgrac100, non-inducible vector, Bacillus subtilis.
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Q&A for students and Interview outstanding student session
11.40 ~ 12.15