Organization of the Cell
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Transcript of Organization of the Cell
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Organization of the CellOrganization of the Cell
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Cell Theory
Cells are the basic living Cells are the basic living units of organization and units of organization and
function in all organisms and function in all organisms and all cells come from other all cells come from other
cellscells
Cells are the basic living Cells are the basic living units of organization and units of organization and
function in all organisms and function in all organisms and all cells come from other all cells come from other
cellscells
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Cell TheoryThe players:
Matthias Schleiden- German botanist (1838)
Theodor Schwann- German zoologist (1839)
Rudolph Virchow- German professor of pathology (1855)
The players:
Matthias Schleiden- German botanist (1838)
Theodor Schwann- German zoologist (1839)
Rudolph Virchow- German professor of pathology (1855)
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Schleiden and Schwann
The first to point out that all plants and animals are composed of cells.The first to point out that all plants and animals are composed of cells.
1838
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Rudolph Virchow
The first to observe cells dividingThe first to observe cells dividing
1855
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History of the Microscope
• Robert Hooke examined a thin piece of cork using a compound microscope- noticed the boxes in the thin slice and called them “cells”
• Robert Hooke examined a thin piece of cork using a compound microscope- noticed the boxes in the thin slice and called them “cells”
?
1665
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History of the MicroscopeAnton van Leeuwenhoek viewed living cells with 200 magnification single lenses of his own construction. His important discoveries include bacteria, protists, blood cells, and sperm cells.
Anton van Leeuwenhoek viewed living cells with 200 magnification single lenses of his own construction. His important discoveries include bacteria, protists, blood cells, and sperm cells.
1670sDutch ScientistDutch Scientist
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Van Leeuwenhoek’s Microscope
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1800
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1860
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1880
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1890
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1899
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1908
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1930
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1951
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1970
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2004
Nikon ‘confocal’ microscope and, “No, I don’t know how much it costs.”
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Electron Microscope
Invented in 1930s by (believe it or not) German scientists Max Knott and Ernst Ruska
Invented in 1930s by (believe it or not) German scientists Max Knott and Ernst Ruska
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Transmission Electron MicroscopeTransmission Electron Microscope
•2-D Image
•Image not living
•10,000X to 100,000X
•Electron beam passes through the specimen
•Specimen is thinly sliced
•2-D Image
•Image not living
•10,000X to 100,000X
•Electron beam passes through the specimen
•Specimen is thinly sliced
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Scanning Electron MicroscopeScanning Electron Microscope•3-D imaging
•Image not living
•1,000X-10,000X magnification
•Image is coated with a thin film of metal and the electron beams are collected as they bounce off of the specimen
•3-D imaging
•Image not living
•1,000X-10,000X magnification
•Image is coated with a thin film of metal and the electron beams are collected as they bounce off of the specimen
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Prokaryotic Cells
Bacteria are prokaryotic cells. All other known organisms consist of …..
Bacteria are prokaryotic cells. All other known organisms consist of …..
Eukaryotic Cells
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Prokaryotic Cells• Structurally simpler than
eukaryotic cells• Nuclear material not
enclosed in a membrane• Ribosomes smaller than
Euk.• Lack of membrane
bound organelles
• Structurally simpler than eukaryotic cells
• Nuclear material not enclosed in a membrane
• Ribosomes smaller than Euk.
• Lack of membrane bound organelles
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bacteria
Cheek cells
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Eukaryotic Cells• Membrane bound organelles• Cell Nucleus• Ribosomes• Endoplasmic reticulum• Golgi complex• Lysosomes• Peroxisomes• Vacuoles• Mitochondria• Chloroplasts
• Membrane bound organelles• Cell Nucleus• Ribosomes• Endoplasmic reticulum• Golgi complex• Lysosomes• Peroxisomes• Vacuoles• Mitochondria• Chloroplasts
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Membrane Bound Organelles
The ‘stuff’ outside the nucleus and inside the cell membrane, suspended in cytoplasm
The ‘stuff’ outside the nucleus and inside the cell membrane, suspended in cytoplasm
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Membrane Bound Organelles
ribosomesmitochondria
Endoplasmic reticulum
Golgi complexLysosomesPlastids
Just to name a few
Vacuoles
Peroxisomes
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Cell Nucleus
Cell Nucleus
Contains nucleolus and chromosomes (DNA)
Contains nucleolus and chromosomes (DNA)
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The Nucleus
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Cell Nucleus• Typically in the center of the cell
• Most cells have a single nucleus
• Typically in the center of the cell
• Most cells have a single nucleus
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Nuclear Envelope• Controls traffic
between the nucleus and the cytoplasm
• Pores in the nuclear membrane allow materials to pass in and out of the
• nucleus
• Controls traffic between the nucleus and the cytoplasm
• Pores in the nuclear membrane allow materials to pass in and out of the
• nucleus
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Nucleus – a closer look
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Nuclear Envelope
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EM View of Envelope
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A closer look at the envelope
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Nuclear Lamina• Inside the nucleus• Formed by intermediate
filaments• Important in the timing of
the disorganization of the membrane during cell division and the ensuing redevelopment
• Inside the nucleus• Formed by intermediate
filaments• Important in the timing of
the disorganization of the membrane during cell division and the ensuing redevelopment
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Lamina
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Chromatin
• When dividing, DNA takes the form of chromosomes
• When not dividing, the DNA takes a looser form called chromatin
• When dividing, DNA takes the form of chromosomes
• When not dividing, the DNA takes a looser form called chromatin
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Loose Chromatin
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Ribosomal Subunits
• Eukaryotic ribosomal subunits are assembled in the nucleolus
• Ribosomes are composed of two subunits
• Eukaryotic ribosomal subunits are assembled in the nucleolus
• Ribosomes are composed of two subunits
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Ribosomes
• Ribosomes manufacture proteins
• Ribosomes may be free or may be attached to the endoplasmic reticulum
• Ribosomes manufacture proteins
• Ribosomes may be free or may be attached to the endoplasmic reticulum
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Endoplasmic Reticulum
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Endoplasmic Reticulum
• Major manufacturing center- proteins
• Extends from the nuclear membrane into the cytoplasm
• Lumen- the space enclosed by the ER- typical intracellular membrane
• Major manufacturing center- proteins
• Extends from the nuclear membrane into the cytoplasm
• Lumen- the space enclosed by the ER- typical intracellular membrane
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The Cytosol side of the ER may be studded with ribosomes
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Rough ER• Site of protein synthesis
• Proteins formed may be transferred to other sites within the cell in transport vesicles
• Site of protein synthesis
• Proteins formed may be transferred to other sites within the cell in transport vesicles
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Transport vesicles
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Smooth ER• Lacks
ribosomes• Lipid
production• Detoxifying
chemical agent
• Lacks ribosomes
• Lipid production
• Detoxifying chemical agent
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Golgi Complex
• Cis face functions in receiving materials• The Trans face is directed toward the
plasma membrane• Function: processing, sorting and
modifying proteins• The process product is then passed to
other organelles or to the plasma membrane
• Manufactures lysosomes
• Cis face functions in receiving materials• The Trans face is directed toward the
plasma membrane• Function: processing, sorting and
modifying proteins• The process product is then passed to
other organelles or to the plasma membrane
• Manufactures lysosomes
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Golgi Complex- Cis and Trans Face
University of texas medical school
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Convex shape
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Lysosomes
• Compartments for digestion
• Small sacs filled hydrolytic enzymes
• Primary lysosomes bud from the Golgi complex
• Involved in apoptosis (programmed cell death)– Inappropriate apoptosis may be involved in
many different catastrophic illnesses
• Compartments for digestion
• Small sacs filled hydrolytic enzymes
• Primary lysosomes bud from the Golgi complex
• Involved in apoptosis (programmed cell death)– Inappropriate apoptosis may be involved in
many different catastrophic illnesses
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Peroxisomes
• Metabolize small organic compounds
• Transfer hydrogen from various compounds to oxygen, forming hydrogen peroxide
• Catalase splits hydrogen peroxide rendering it harmless
• Metabolize small organic compounds
• Transfer hydrogen from various compounds to oxygen, forming hydrogen peroxide
• Catalase splits hydrogen peroxide rendering it harmless
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Peroxisomes
• Common in cells that synthesize, store, or degrade lipids
• Plant cells have specialized peroxisomes called glyoxysomes
• Common in cells that synthesize, store, or degrade lipids
• Plant cells have specialized peroxisomes called glyoxysomes
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•metabolism of free oxygen radicals; •synthesis of cholesterol and ether lipids; •bile acid formation; •catabolism of long chain fatty acids; •catabolism of purines, prostaglandins, leucotriens; •alcohol detoxification in liver
•metabolism of free oxygen radicals; •synthesis of cholesterol and ether lipids; •bile acid formation; •catabolism of long chain fatty acids; •catabolism of purines, prostaglandins, leucotriens; •alcohol detoxification in liver
Peroxisome Functions:
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Some interesting facts about peroxisomes are: •Human congenital diseases associated w/absence of peroxisomes and/or dysfunction of their enzymes•many chemicals (drugs, industrial pollutants) induce a marked proliferation of peroxisomes; •prolonged Tx w/ most proliferators induce malignant hepatic tumors
Some interesting facts about peroxisomes are: •Human congenital diseases associated w/absence of peroxisomes and/or dysfunction of their enzymes•many chemicals (drugs, industrial pollutants) induce a marked proliferation of peroxisomes; •prolonged Tx w/ most proliferators induce malignant hepatic tumors
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Vacuoles• Large, fluid filled sacs• Carry out variety of functions
– In plants & fungi, vacuoles carry out many of the functions of the lysosome
– Allow plants to increase in size
• Bound by a membranous tonoplast• May store toxins or pigments• Protists have vacuoles that are involved
in digestions and secretion
• Large, fluid filled sacs• Carry out variety of functions
– In plants & fungi, vacuoles carry out many of the functions of the lysosome
– Allow plants to increase in size
• Bound by a membranous tonoplast• May store toxins or pigments• Protists have vacuoles that are involved
in digestions and secretion
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Mitochondria• Energy converting organelle- site of aerobic
respiration• Double membrane bound• Matrix- inside of the inner membrane• Cristae- the foldings of the inner membrane,
providing a large surface area• Mutations in mitochondrial DNA have been
linked to several genetic diseases• Mitochondria also affect health by leaking
electrons, which form free radicals, into the cell
• Energy converting organelle- site of aerobic respiration
• Double membrane bound• Matrix- inside of the inner membrane• Cristae- the foldings of the inner membrane,
providing a large surface area• Mutations in mitochondrial DNA have been
linked to several genetic diseases• Mitochondria also affect health by leaking
electrons, which form free radicals, into the cell
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Outer membrane lets manyMolecules through, but inner membrane is very selective
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Chloroplasts
• Convert light energy into chemical energy through photosynthesis
• Pigments like chlorophylls are specialized for photosynthesis
• Double membrane bound• Develop from proplastids• Chromoplasts contain pigments and are
common in petals and ripe fruit• Leukoplasts lack pigments and may
store starch
• Convert light energy into chemical energy through photosynthesis
• Pigments like chlorophylls are specialized for photosynthesis
• Double membrane bound• Develop from proplastids• Chromoplasts contain pigments and are
common in petals and ripe fruit• Leukoplasts lack pigments and may
store starch
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Contains enzymes
responsible for producing
carbohydrates from carbon dioxide and
water
Fluid filled area
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Thylakoids are involved with producing ATP. This is where the chlorophyll is.
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Would you recognize these structures as chloroplasts? How?
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amyloplasts
Proplastids that will turn into
amyloplasts
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Cytoskeleton
• Provides for cell shape and allows movement
• Classifying elements of cytoskeleton by size– Microfilaments- smallest
– Intermediate filaments
– Microtubules- largest
• Provides for cell shape and allows movement
• Classifying elements of cytoskeleton by size– Microfilaments- smallest
– Intermediate filaments
– Microtubules- largest
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Hollow cylinders
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Microtubules are in green. Actin is in red. DNA is blue.
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MTOCMicrotubule Organizing Center- CentrosomeMicrotubule Organizing Center- Centrosome
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http://www.cellsalive.com/mitosis.htm
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Dynein
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Cilia and Flagella
• Composed of microtubules• Cilia- numerous and short• Flagella- longer and fewer in number• Move the cell or move substances over
the surface of the cell• Both have 9+2 arrangement of
microtubules• Basal body and centrioles have 9X3
arrangement
• Composed of microtubules• Cilia- numerous and short• Flagella- longer and fewer in number• Move the cell or move substances over
the surface of the cell• Both have 9+2 arrangement of
microtubules• Basal body and centrioles have 9X3
arrangement
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9 + 2 arrangement of cilia
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Microfilaments
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microtubules
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actin
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Extracellular Matrix
• ECM
• Secreted gel surrounding cell
• Composed of collagen which forms very tough fibers
• Integrins- main membrane receptors for the ECM
• Help the cell cell signaling pathways and help regulate various functions of the cell
• ECM
• Secreted gel surrounding cell
• Composed of collagen which forms very tough fibers
• Integrins- main membrane receptors for the ECM
• Help the cell cell signaling pathways and help regulate various functions of the cell
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Glycocalyx- Cell Coat
• Surround most eukaryotic cells
• Formed by polysaccharide side chains
• May act as recognition sites
• Surround most eukaryotic cells
• Formed by polysaccharide side chains
• May act as recognition sites
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Junctions between Cells• Form strong connections• Prevent passage of materials• Establish communication • Animal cell junctions
– Anchoring (desmosomes and adhering junctions– Tight junctions– Gap junctions
• Plant Cell junction– plasmodesmata
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Des
mos
omes
• Points of attachment for some animal cells
• Hold cells subject to mechanical stress together
• Composed of intermediate filaments, which span the gap between two cells
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Cell Walls
• Surround plant, fungal, and bacterial cells
• Primary cell wall can expand as the cell grows
• Secondary cell wall forms between the primary cell wall and the cell membrane
• Middle lamella glues adjacent plant cells together
• Surround plant, fungal, and bacterial cells
• Primary cell wall can expand as the cell grows
• Secondary cell wall forms between the primary cell wall and the cell membrane
• Middle lamella glues adjacent plant cells together
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Middle Lamella
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