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8 October 2020 · 16:00–17:00 CET
Spotlight on ...Unlocking the potential of industrial yeasts for production of high value consumer products
DigitalSupporting PartnerOrganised by
Hosted by:
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DigitalSupporting PartnerOrganised by
SPEAKERS
John Morrissey – CHASSY Project Coordinator, Senior Lecturer in Microbiology, University College Cork, Ireland
Else-Jasmijn Hassing – PhD candidate, Delft University of Technology, Netherlands
Florian David – CSO & Co-Founder, Biopetrolia AB, Gothenburg, Sweden
Tristan Rossignol – Senior Researcher, MICALIS Institute at INRAE, France
Verena Siewers – Senior Researcher, Chalmers University of Technology, Gothenburg, Sweden
Hosted by:
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
1. Introduction to CHASSY (John Morrissey, UCC)……………............................4
2. Yeast platforms for aromatics (Jasmijn Hassing, TUD)……........................14
3. Yeast platforms for fatty acids and derived products (Florian David, Biopetrolia)………………………………………………………………………………………….…24
4. Applications of Kluyveromyces marxianus for food and industrial biotechnology (John Morrissey, UCC) ……………………………………………………33
5. Yarrowia lipolytica as a platform for sustainable lipids production (Tristan Rossignol, INRAE)………………………………………………………………………45
6. Computational models of industrial yeast metabolism and their role in changing the landscape of industrial biotechnology (Verena Siewers, Chalmers)……………………………………………………………………………………………….53
Table of contents
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Edit title
Unlocking the potential of industrial yeasts for production of high value consumer products
European Forum for Industrial Biotechnology & the Bioeconomy (EFIB)
8th October 2020
Dr John MorrisseyUniversity College Cork [email protected]
Session Moderator Speakers and Panel
Florian David, SwedenTristan Rossignol, FranceJasmijn Hassing, NetherlandsJohn Morrissey, IrelandVerena Siewers, SwedenJean-Marc Nicaud, France
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Funded by the European Union in H2020 under the Leadership in Enabling and Industrial Technologies (LEIT) Pillar (2017 – 2021)
Model-Based Construction And OptimisationOf Versatile Chassis Yeast Strains For ProductionOf Valuable Lipid And Aromatic Compounds
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 720824
Microbial chassis platforms with optimised metabolic pathways for industrial innovations through systems biology
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Development of efficient cell factories for the industrial production of novel high-value products
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 720824
Dr John MorrisseyUniversity College Cork CHASSY Project [email protected]
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Development of efficient cell factories for the industrial production of novel high-value products
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 720824
Dr John MorrisseyUniversity College Cork CHASSY Project [email protected]
Lead ScientistsJohn MorrisseyJean-Marc DaranJack PronkVerena SiewersJens NielsenTristan RossignolJean-Marc NicaudCeline HenryMislav OrebEckhard BolesFlorian DavidAnastasia KrivoruchkoHenning Markmann
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Unlocking the potential of industrial yeasts for production of high value consumer products
Traditional Products
Fermented BeveragesEthanolCitric AcidEnzymesFragrancesFlavours
Biopharma Products
InsulinGrowth factorsVaccinesEnzymes
Artemsinin
Bio-economy products
2G ethanolLactic AcidSuccinic acid
Flavours
Wide range of yeastsTorulaspora delbrueckii
Kluyveromyces lactisKluyveromyces marxianusSaccharomyces cerevisiaeDebaryomyces hansenii
Yarrowia lipolytica
Saccharomyces cerevisiae
Saccharomyces cerevisiae
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Unlocking the potential of industrial yeasts for production of high value consumer products
Traditional Products
Fermented BeveragesEthanolCitric AcidEnzymesFragrancesFlavours
Bio-economy products
2G ethanolLactic AcidSuccinic acid
Flavours
Wide range of yeastsTorulaspora delbrueckii
Kluyveromyces lactisKluyveromyces marxianusSaccharomyces cerevisiaeDebaryomyces hansenii
Yarrowia lipolytica
Saccharomyces cerevisiae
Other Industrial hosts?
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From: Application of synthetic biology for production of chemicals in yeast Saccharomyces cerevisiaeFEMS Yeast Res. 2015;15(1):1-12. doi:10.1111/1567-1364.12213
Yeast offer huge potential for production of bio-molecules, but ……..
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
From: Application of synthetic biology for production of chemicals in yeast Saccharomyces cerevisiaeFEMS Yeast Res. 2015;15(1):1-12. doi:10.1111/1567-1364.12213
Yeast offer huge potential for production of bio-molecules, but ……..
• Strain construction is increasingly complex
• Time for strain development is too slow
• Cost of development is prohibitive
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
From: Application of synthetic biology for production of chemicals in yeast Saccharomyces cerevisiaeFEMS Yeast Res. 2015;15(1):1-12. doi:10.1111/1567-1364.12213
CHASSY focus: overcoming bottlenecks to accelerate innovation
“We offer an innovative platform and toolbox
for accelerated delivery of high-value yeast-
derived products for the bio-economy”
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Outputs from CHASSY that can accelerate industrial innovation
Systems strategies for
optimisng yeast for
biololecule production
Florian DavidBiopetroliaSweden
Jasmijn HassingTU DelftNetherlands
John MorrisseyUCCIreland
Tristan RossignolINRAEFrance
Verena SiewersChalmersSweden
Product Classes New Platforms New Thinking
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Edit title
Yeast platforms for aromatics
Model-Based Construction And OptimisationOf Versatile Chassis Yeast Strains For ProductionOf Valuable Lipid And Aromatic Compounds
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 720824
Jasmijn Hassing, Delft University of Technology, Netherlands
October 2020
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Edit titleModel-Based Construction And OptimisationOf Versatile Chassis Yeast Strains For ProductionOf Valuable Lipid And Aromatic Compounds
One
(aromatic)
Ring to make
them all…
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
One Ring to make them all
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Exploiting the market
2-phenyl
ethyl acetate
500 tonnes
~4.5 USD/kg
Flavonoids
410 M USD
7-90 USD/kg
Vanillin
18000 tonnes
60-3300 €/kg
Lignans
70 M USD
~375 USD/kg
2-phenylethanol
5000 tonnes
350 USD/kg
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
- Unstable/limited supply- Mixed compounds- Difficult extraction+ Natural
+ Stable/Unlimited supply
- Limited products- Harsh conditions- Not natural
+ Stable/Unlimited supply+/- Pure compounds+ Natural
Production methods
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Microbial production
Phenylalanine(Tyrosine)
Malonyl-Coa
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Yeast chassis
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Our yeast chassis
Kluyveromycesmarxianus
Thermotolerant
Wide substraterange
> 0.8 g L-1
precursor
Yarrowialipolytica
Oleaginous
Good secretion
> 0.6 g L-1
precursor
Saccharomycescerevisiae
Robust
Well characterized
> 1.5 g L-1
precursor
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
We offer
Chassis strains Know-how on strain engineering
Collaborations
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Contact us
Jean-Marc Daran [email protected] (PI – Saccharomyces)
Else-Jasmijn Hassing [email protected] (Phd)
John Morrissey [email protected] (PI – Kluyveromyces)
Jean-Marc Nicaud [email protected] (PI – Yarrowia)
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www.pulp2value.eu www.chassy.euEFIB, 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Model-Based Construction And OptimisationOf Versatile Chassis Yeast Strains For ProductionOf Valuable Lipid And Aromatic Compounds
EFIB, October 2020
Assist. Prof. Florian David CSO, Biopetrolia [email protected]
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 720824
Yeast platforms for fatty acids and derived products
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Why an alternative for fatty acid production?
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Our Solution Fatty acids through Yeast Biotechnology
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Key advantages of yeast-based production of fatty acid-derived products
Sustainable sourcingProduction from wide-variety of feedstocks (incl. waste/side-streams), resulting in reduced land use and greenhouse gas emissions
EconomicCost-efficient alternative to production of many fatty acid-derived products.
€
Tailor made structuresSpecific design parameters (chain length, saturation, assembly into complex products, etc.)à specialized properties for specific applications
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Our technology platformRapid engineering of yeast cell factories
Fatty acids
Cell mass
Yeast strains producing g/L of
fatty acids
We can rapidly optimize production and create industrially-relevantstrains for production of many different fatty acid-based products.
LEARN
Different organism can provide different benefits:Saccharomyces cerevisiae: robust, well characterized, easy to engineerYarrowia lipolytica: naturally high fat production
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www.pulp2value.eu www.chassy.euEFIB, 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Fatty AcidsStarter molecules for a lot of products
Fuels
Cosmetics
Fragrances
Nutraceuticals
AgrochemicalsAldehydes, acetates, alcohols
Alkanes, Alkenes, FAEE
Waxes, long chain alcohols
Food ingredients Triacylglycerols SC alcohols, aldehydes, lactones
VLC fatty acids, PUFA
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www.pulp2value.eu www.chassy.euEFIB, 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Hydrocarbons$600B/yr
Adipic acid$6B/yr
Dodecanedioic acid
$400M/yrSemiochemicals
>$540M/yr
Musks$180M/yr
Fatty alcohols
$5B/yr
PUFAs$4B/yr
MCT oil$1.6B/yr
VLC wax esters$900M/yr
Phospholipids$4B/yr
Vegetable oil(Palm & others)
$180B/yr
Vegetable oil(Palm & others)
$180B/yr
Wide spectrum of market opportunitiesFatty acid-based products are used across a wide spectrum of market segments for a
wide array of applications worth $billions/yr
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www.pulp2value.eu www.chassy.euEFIB, 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Chassy Platform Strains
Developed platform strains Applications for platforms
Example products
S. cerevisiae strains producing high level of fatty acids and derived products (C16/C18)
Food fats/oils, biofuels, building blocks, personal care.
Palmitic acid, oleic acid etc.
S. cerevisiae strains producing high level of very long chain fatty acids and derived products (>C18)
Nutraceuticals, personal care, pharmaceuticals.
Docosanol
S. cerevisiae strains producing high level of short chain fatty acids and derived products (<C16)
Personal care, food ingredients, pharmaceuticals.
Octanoic acid, Hydroxyoctanoic acid
These platform strains are ideal starting points to develop a variety of fatty acid and fatty acid-derived producing strains.
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www.pulp2value.eu www.chassy.euEFIB, 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Contact usBiopetrolia
[email protected]@biopetrolia.com
Info on short-chain fatty acids:
Goethe University FrankfurtDr Eckhard Boles
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Edit title
Applications of Kluyveromyces marxianus for food and industrial biotechnology
Model-Based Construction And OptimisationOf Versatile Chassis Yeast Strains For ProductionOf Valuable Lipid And Aromatic Compounds
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 720824
John Morrissey, University College Cork
October 2020
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Kluyveromyces marxianus• QPS and GRAS status• “Milk”, “dairy” or “lactic” yeasts• Involved in traditional dairy fermentations• Putative health benefits
QPS status (qualified
presumption of safety)
Kluyveromyces yeasts have a long history in traditional food and beverages
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Examples of industrial use of K. marxianus1 – Bioethanol from whey permeates
yeast
Ethanol
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Examples of industrial use of K. marxianus2 – Bioconversion of amino acids to flavours
2-phenylalanine
2-phenylethanol
OH
O
O
O
NH2
OH
O
OH
L-phenylalanine
O
O-
O
O
OH
LeucineNH2
OH
OO
O-
O
O
O-
OH
OH
2-Phenylethylacetate Isoamyl acetate
Market data
5,000 tonnes
350 USD/kg (natural 2-PE)
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• Thermotolerant (up to 52oC)
• Short generation time (as low as 45 min)
• Assimilates lactose, cellobiose and xylose
• GRAS / QPS
Kluyveromyces marxianus – a versatile yeast for industrial biotechnology
CHALLENGES AND LIMITATIONS?
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Knowledge of strain physiology
Molecular tools for strain engineering
Host strains
Exemplar strains to demonstrate utility
Scale-up experience and expertise
Challenges to developing K. marxianus for IB applications
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Knowledge of strain physiology
Challenges to developing K. marxianus for IB applications
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Molecular tools for strain engineering
Host strains
Challenges to developing K. marxianus for IB applications
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CRISPR – based genome engineering
Development and application of tools for engineering K. marxianus
• Optimised host strains
• Gene engineering pipelines
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Synthetic Biology infrastructure – pathway construction
Development and application of tools for engineering K. marxianus
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
A case study: de novo synthesis of 2- Phenylethanol (2-PE) in K. marxianus
Shikimate pathway
2- Phenylethanol~ 750mg/L 2-PE
Flask culture
AAA pathway
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
New industrial host
Wide substrate range
Capacity for aerobic production of aromatics
Amenable to genetic manipulation and synthetic biology
The offer from CHASSY
Know-how to construct cell factory strains
Knowledge of genetics, physiology and metabolism
Optimised strains and methods for aromatics
Collaborative partnerships to develop strains and processes
What is this of interest to industry?
Contact
Dr John Morrissey
University College Cork
Email: [email protected]
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Edit title
Yarrowia lipolytica as a platform for sustainable lipids production
Model-Based Construction And OptimisationOf Versatile Chassis Yeast Strains For ProductionOf Valuable Lipid And Aromatic Compounds
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 720824
Tristan Rossignol, INRAE France
EFIB 2020
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Yarrowia lipolytica
Oleaginous yeast
Accumulates high amount of lipids
Grows on hydrophobic substrates
Isolated from lipid-rich or protein-rich environments
GRAS status (generally regarded as safe)
QPS status (qualified presumption of safety)
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Yarrowia lipolytica, an industrial chassis
Eicosapentaenoic acid (omega 3)
Feeding of farm animalsSingle cell protein1970’s
Long history in industry
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
image from www.pngfuel.com
Sustainable & environmentally friendly production
Host for biolipids production
Lipid metabolism well known
Naturally a good chassis for biolipids production
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Metabolic Engineering
Carbon sources
Biolipids
Development of chassis optimized for acetyl-CoA precursors
Modification of metabolic pathways
Heterologous pathway “plug-in”
Increase quantityControl composition
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Expanding genetic engineering tools
New versatile and efficient tools in the frame of CHASSY dedicated to synthetic biology
Gene targeting Metabolic pathway insertion
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Metabolic Engineering
Carbon sources
Biolipids
Acetyl-CoA
(6.5 g/L) Larroude et al 2018
Glucose
Glycerol
Lipidic wastes
Hemicellulose
Starch
Molasses
Carotenoid pathway
Biofuels
Odd-chain fatty acids Ricinoleic acids
Cyclopropane fatty acids Poly unsaturated fatty acids
Acetylated triglyceride….
Unusual lipids
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Yarrowia lipolytica, an industrial chassis for biolipids production
GRAS - QPS
Robust industrial organism (pH etc)
High potential for biolipids and acetyl-CoA derivatives production
Contact
Dr Jean-Marc Nicaud
INRAE – Micalis, Jouy-en-Josas
Email: [email protected]
Dr Tristan Rossignol
INRAE – Micalis, Jouy-en-Josas
Email: [email protected]
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Edit title
Model-Based Construction And OptimisationOf Versatile Chassis Yeast Strains For ProductionOf Valuable Lipid And Aromatic Compounds
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 720824
Verena Siewers, Chalmers University of Technology, Gothenburg, Sweden
8 October 2020
Computational models of industrial yeast metabolism and their role in changing
the landscape of industrial biotechnology
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Design-build-test-learn cycle
Genome-scale metabolic models(GEMs) assist in strain design
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Genome-scale metabolic models
Each gene and reaction is represented in a stoichiometric matrix
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Genome-scale metabolic modelsH
igh
-val
ue
pro
du
ct
Glucose Xylose
Ove
rflo
w m
eta
bo
lite
• Determine maximum biochemical yield
• Evaluate alternative pathways
• Estimate internal flux distributions
• Predict beneficial genetic manipulations
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Use of GEMs for cell factory design
Use of GEM to select target genes for CRISPR-based regulation
Increase of 3-hydroxypropionic acid production
ACS Synth Biol (2019) 8, 2457–2463
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Use of GEMs for cell factory design
Problem 1: Spatial constraint
Limited amount of total protein per cell (enzyme mass not accounted for in model)
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Problem 2: Varying enzyme efficiency
Use of GEMs for cell factory design
Pathway YZ requires less proteinPathway AB is faster
Hig
h-v
alu
e p
rod
uct
Glucose
A
B
Z
Y Enzyme Size Reaction rate
A 33 kDa +++
B 312 kDa +++
Y 27 kDa +
Z 33 kDa +
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Use of GEMs for cell factory design
S. cerevisiaeK. marxianusY. lipolytica
Solution: GECKOEnzyme amounts and catalytic enzyme efficiencies included in the model
ecModels for 3 CHASSY yeasts:
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Use of GEMs for cell factory design
Model prediction Exp. Measurement Decision
(high product yield) (low product yield)
• Deletion of non-essential enzymes
• Fine tuning levels of key enzymes
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Use of GEMs for cell factory design
Modelling pipeline:Used for prediction of genetargets for 110 industriallyrelevant chemicals
• Alcohols• Alkaloids• Amino acids• Aromatics• Amines• Fatty acids• Flavonoids• Isoprenoids/steroids• Organic acids• Proteins
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www.pulp2value.eu www.chassy.euEFIB 2020: Unlocking the potential of industrial yeasts for production of high value consumer products
Contact us
[email protected]@[email protected]
Systems and Synthetic BiologyChalmers University of Technology
Gothenburg, Sweden