Intracellular comparment

Percentage total cell volume

Cytosol 54

Mitochondria 22

Rough ER 9

Smooth ER + Golgi

6

Nucleus 6

Peroxisomes 1

Lysosomes 1

Endosomes 1

Plasma 2 5

Rough ER 35 60

Smooth ER 16 <1

Golgi 7 10

Mito. Out. 7 4

Mito. Inner 32 17

Nucleus 0.2 0.7

Sec. vesicle

n.d. 3

Lysosome 0.4 n.d.

Peroxisom 0.4 n.d.

Endosome 0.4 n.d.

Destination Sample Sequence

Import to nucleus P-P-K-K-K-R-V

Export from nucleus L-A-L-K-L-A-G-L-D-I

Import to mitochondria +H3N-M-L-S-L-R-N-S-I-R-F-F-K-P-A-T-R-T-L-C-S-S-R-Y-L-L

Import to plastid +H3N-M-V-A-M-A-M-A-S-L-N-S-S-M-S-S-L-S-L-S-S-N-S-F-L-G-N-P-L-S-P-I-T-L-S-P-F-L-N-G

Import to peroxisomes S-K-L-COO-

Import into ER +H3N-M-M-S-F-V-S-L-L-L-V-G-I—L-F-W-A-T-E-A-E-N-L-T-K-C-E-V-E-N

Return to the ER K-D-E-L-COO-