Optimization designs Dr.yasser/RSM_Box... · 2009. 10. 14. · Abdel-Fattah and Sheta 2003. Genetic...
Transcript of Optimization designs Dr.yasser/RSM_Box... · 2009. 10. 14. · Abdel-Fattah and Sheta 2003. Genetic...
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• Three-level designs• Polynomial structured model• Non-linear optimization algorithm to find out where the optimumis likely to be located
• Verification of the predicted model by experiment
Optimization designs
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Response surface methodology: BoxResponse surface methodology: Box--Behnken DesignBehnken Design
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Experimental resultsExperimental results
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How to do the analysis on PC ??How to do the analysis on PC ??
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YPCR yield = 189.3+2.5*primer+26.44*cycles+18*Taq+5.2*Mg+3.9*Annealing-5.1*Extension-0.25*primer*cycles-17.84*primers*Taq-10.14*cycles*Taq-10.58*primers*Mg+7.8*cycles*Mg-24.85*Taq*Mg+8.4*primers*Extension-11.96*cycles*Annealing+9.4*Taq*Annealing-3.14*Mg*Annealing+0.48*primers*Extension+0.05*cycles*Extension-13.4*Taq*Extension-20.64*Mg*Extension-8.7*Annealing*Extension-0.18*primers*primers-7.18*cycles*cycles-10.77*Taq*Taq-29.9*Mg*Mg+5.86*Anealing*Anealing-28*Extension*Extension
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يتوفر لدي وحدة الخدمات المرآزية بالمدينة يتوفر لدي وحدة الخدمات المرآزية بالمدينة 100100 bpbp DNA Marker (100DNA Marker (100--2500bp)2500bp)
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100 bp
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750 bp1000 bp1500 bp2000 bp2500 bp
Agarose gel electrophoresis (2%) with 1-5 μl of the DNA marker
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Commercial software available free on WWWCommercial software available free on WWW
httphttp://://wwwwww..geocitiesgeocities..comcom//SiliconValleySiliconValley//NetworkNetwork//10321032//
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Applications of statistical experimental designsApplications of statistical experimental designs
1. Pharmaceutical industry: Tablet formulation and chromatographic methods
2. Environmental biotechnology: waste water treatment and bioremediation
3. Chemical industry: optimize reaction conditions for maximum yield
4. Bioprocess development: Optimization of fermentation yield includingenzymes, antibiotics, biopolymers as will as many secondary metabolites
5. Molecular biology: PCR optimization and micro array
What is the next level in experimental design???What is the next level in experimental design???
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Evolutionary computationEvolutionary computationNon-model based optimization method, with the ability to search A system with a large number of parameters in directed wayHibbert 1993
Has been applied on formate dehydrogenase enzyme productionWeuster-Botz and Wandery 1995
Has been applied on thermostable Lipase production model with higherDegree of fitness to experimentalData.Abdel-Fattah and Sheta 2003
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Genetic Engineering and Biotechnology Research Institute
GEBRIGEBRI
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