(on the Wecoma)

Wecoma Cruise Schedule

Wecoma Cruise Schedule (week 1)

Wecoma Cruise Schedule (week 2)

Wecoma Cruise Schedule (week 3)

Wecoma Cruise Coastal Lines

Water samples

Sediment samples

CTD profiles (all stations)

* Sediment samples can also be collected on Strawberry Hill and Cape Meares lines

Water sample analyses

• DNA & RNA samples (30 L per sample)– Community fingerprinting– Transcriptomics

• CARD-FISH filter preparation (0.05 L)• Chemistry (5 L)

– Dissolved Inorganic Nutrients: NO3+NO2, NO2, NH4, PO4

– Dissolved Organic Carbon– Particulate Organic Carbon and Nitrogen – Chlorophyll a – Suspended Particle Mass in estuary and plume only

• Bacterial Production (0.1 L)• Bacterial and Total Respiration (5 L)• Bacterial Abundance (0.02)

Sediment analyses

• Community fingerprinting• Transcriptomics• Membrane lipid analysis• Characterization of geochemical gradients

Wecoma Cruise Scientific Personnel

Name Inst. Activity Watch 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 1 Levine, Murray OSU mooring Day X Waldorf, Walt OSU mooring Day X Risien, Craig OSU mooring Day X Wet Labs Person mooring Day X Crump, Byron UMCES Chief Sci Day X X X X X X X X X X X X X X X X X X X Apple, Jude UMCES water Day X X X X X X X X X Green, Joanna UMCES water Day X X X X X X X X X X Fortunato, Caroline UMCES water Day X X X X X X X X X X X X X X X X X X X NEED PERSON OHSU water Day X X X X X X X X X X X X X X X X X X X Sheehan, Carolyn OHSU water Day X X X X X X X X X X X X X X X X X X Murphy, Daniel OHSU water Day X X X X X X X X NEED PERSON X X X X X X X X X X NEED PERSON OSU CTD Day X X X X X X X X X X X X X X X X X X X NEED PERSON OSU CTD Night X X X X X X X X X X X X X X X X X X X Hubbard, Dale OSU coring Day X X X X X X X X X Tratnyek person OHSU coring Day X X X X X X X X X X X X X X X X X X X O’Gorman, Dave OSU Mar Tech Day X X X X X X X X X X X X X X X X X X X Newell, Sonya OSU Mar Tech Night X X X X X X X X X X X X X X X X X X X Open Berth O O O O O O O O O O O O O O O O O O Open Berth O O O O O O O O O O O O O O O O O O Open Berth O O O O O O O O O O

Loading: Aug 13

• Mooring equipment (Murray Levine, Walt Waldorf, Craig Risien, Wet Labs person)

• Coring equipment (Dale Hubbard)• CTD setup & sensor installation (Dave O’Gorman,

Sonia Newell, Michael Wilkin)• DNA/RNA filtering station (Daniel Murphy, Carolyn

Sheehan, MAYBE NEED PERSON?)• Nutrients, POC/N, chlorophyll filtering stations

(Carolyn Fortunato, NEED PERSON)• Bacterial production & respiration stations (Jude

Apple)

Leg 1: Aug 14-15 (mooring)

• Mooring placement (Murray Levine, Walt Waldorf, Craig Risien, Wet Labs person)

• CTD daytime (Dave O’Gorman, NEED PERSON)• CTD nighttime (Sonya Newell, NEED PERSON)• Practice water sample collection and processing

– Practice DNA/RNA processing (Daniel Murphy, Carolyn Sheehan, MAYBE NEED PERSON?)

– Practice POC/N filtration, nutrients (Carolyn Fortunato)– Practice chlorophyll filtrations (NEED PERSON)– Practice bacterial productions and respirations (Jude Apple)

• 0 empty berths

Leg 2: Aug 15-23 (Coast & plume)

• CTD daytime (Dave O’Gorman, NEED PERSON)• CTD nighttime (Sonya Newell, NEED PERSON)• Water sample collection (everyone)• DNA/RNA processing (Daniel Murphy, Carolyn

Sheehan, MAYBE NEED PERSON?)• POC/N filtration, nutrients (Carolyn Fortunato)• Chlorophyll filtrations (NEED PERSON)• Bacterial production and respiration (Jude Apple)• 2 empty berths

Leg 3: Aug 23-31 (Estuary & plume)

• CTD daytime (Dave O’Gorman, NEED PERSON)• CTD nighttime (Sonya Newell, NEED PERSON)• Water sample collection (everyone)• DNA/RNA processing (NEED PERSON, Carolyn

Sheehan, MAYBE NEED PERSON?)• POC/N filtration, nutrients (Carolyn Fortunato)• Chlorophyll filtrations (NEED PERSON)• Bacterial productions and respirations (Joanna Green)• 4 empty berths

Issue 1: Post-Cruise

• Need Shore-side Assistance with sample transport - someone to meet the ship– DNA/RNA samples - Transport to OHSU in coolers of dry ice.– POC/N, Chlorophyll, TDN/TDP, DOC, CARD-FISH, SPM -

Transport to OHSU in coolers of dry ice. – Nutrient samples - Transport to OSU in coolers of dry ice.

• Shipping to Maryland - need to figure out how to do this• Radioisotope - OSU rad safety will meet ship and mail

samples to Maryland.

Issue 2: Communications

• E-mail with shipboard address via Quick pidgeon messenger service

• Internet - expensive, discouraged

• Ship-to-ship communication?

• Telephone?

• Cell Phones?

Issue 3: Limited Expertise

• Scientific Leadership– 1 PI (Crump)– 1 post-doc (Apple, 1/2 of cruise)

• Technical expertise– CTD?

Issue 4: Site selection

• Use Corie output to pre-select sampling stations

• Coastal sites - span upwelling fronts or other transition zones

• Plume - span the plume front (2-4 times in 2 different 2-day periods

• Estuary - sample surface waters at specific salinities, identify with surface flow-through system

Issue 5: Estuary operations

• Estuary operations (3 days)– Goal - 3 river-ocean transects across salinity

gradient while Barnes samples ETMs– 10 to 15 samples per transect– Only sample during day– Cannot sample at peak tides

• Can we predict the salinity field in the estuary to estimate when and where to sample?

• What do we do at night?

Cruise goals

• Mooring placement at station NH-10 along the Newport Hydroline.

• Occupy a grid of stations over the Oregon and Washington continental shelf and slope to map hydrographic, bio-optical, biological and chemical parameters. – Identify and characterize gradients– Relate gradients to patterns in microbial activity, community

composition, and genetic expression– Combine these data with ADCP measurements of

subsurface velocity to explore biophysical interactions.

• Make continuous measurements of surface water chemistry for higher resolution mapping.

Micro cruise goals

• Collect water samples for DNA- and RNA-based microbial community analyses across environmental gradients in pelagic environments of the Columbia River estuary, the Columbia River plume, and along established sampling lines along the Oregon and Washington coasts.

• Collect sediment samples with the multicorer at select sites along the Newport Hydroline and the Columbia River Line to characterize surface sediment microbial groups of special interest such as Crenarchaeota or Anammox bacteria.

Questions

• How are planktonic & benthic microbial diversity and activity distributed across river-estuary-coastal ecosystems?

• What gradients influence microbial diversity, activity & gene expression?

• How does diversity relate to temporal stability in an environment? (i.e., at what point does a transition zone become a stable environment?)

Goals

• Use nowcast/forecast system to locate environmental gradients and select sampling sites.

• Use ship to ship communication to coordinate ETM sampling.

Coastal Margin Science: Microbial Communities in Productive Coastal Margins

• Determine how microbial populations form across the physical and chemical gradients of the Columbia River river-to-ocean system and to uncover the microbial-mediated activities therein.

• Central theme of cruises• Too many samples? Cruise too long?

Coastal Margin Science: Ecosystem Dynamics, Climate and Water Use

• Describe physical characteristics of the estuary and plume– Are these cruises contributing to this goal?

• Provide guidance for microbial sampling– In the works

• Ecosystem dynamics, biological productivity, air-sea carbon fluxes– Not well addressed. No surface mapping of O2,

CO2, and pigments. No primary production. No zooplankton sampling.

Enabling Technologies: Sensors

• No sensors being tested (yet)

• Nucleic acid hybridization array– Cruise provides data for array development

• Airborne remote sensing– Collecting data during cruise

Enabling Technologies: Information and Visualization