OMICS, Comics and Metaproteomics -...
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OMICS, Comics and Metaproteomics:
Analyzing Microbiomes using proteomics
Pratik Jagtap
@ Saturday Meet Programme
DEPARTMENT OF MICROBIOLOGY
SAVITRIBAI PHULE PUNE UNIVERSITY
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• Omics & Multiomics: Benefits and Challenges
• Functional microbiome analyses
• Metaproteomics, Galaxy and Projects
• Extending tools for metaproteomics
• Microbiology and Microbiome
OMICS, Comics and Metaproteomics:
Analyzing Microbiomes using proteomics
![Page 3: OMICS, Comics and Metaproteomics - Galaxy-Pgalaxyp.org/wp-content/uploads/2019/01/OmicComics_Pune_PJagta… · For example, Protein Database Downloader downloads ... Sci Rep. (2018)](https://reader035.fdocuments.us/reader035/viewer/2022070913/5fb47e2c6b7ef57a0828339a/html5/thumbnails/3.jpg)
https://wildlifesnpits.wordpress.com/2015/11/18/transcriptomics-for-conservation/
Mias and Snyder, Quant Biol. (2013) 1(1): 71–90. doi: [10.1007/s40484-013-0005-3]
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Mass Spectrometry and Proteomics
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Multiomics / trans-omics
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https://doi.org/10.1007/978-1-4939-7717-8_7
Multiomics / trans-omics
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multiomics data: Analytical challenges
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• Omics & Multiomics: Benefits and ChallengesMultiomics Research is emerging and will offer a more complete biological perspective. Multiomicsdata analysis presents challenges and opportunities.
• Functional microbiome analyses
• Metaproteomics, Galaxy and Projects
• Extending tools for metaproteomics
• Microbiology and Microbiome
OMICS, Comics and Metaproteomics:
Analyzing Microbiomes using proteomics
![Page 9: OMICS, Comics and Metaproteomics - Galaxy-Pgalaxyp.org/wp-content/uploads/2019/01/OmicComics_Pune_PJagta… · For example, Protein Database Downloader downloads ... Sci Rep. (2018)](https://reader035.fdocuments.us/reader035/viewer/2022070913/5fb47e2c6b7ef57a0828339a/html5/thumbnails/9.jpg)
• Omics & Multiomics: Benefits and ChallengesMultiomics Research is emerging and will offer a more complete biological perspective. Multiomicsdata analysis presents challenges and opportunities.
• Functional microbiome analyses
• Metaproteomics, Galaxy and Projects
• Extending tools for metaproteomics
• Microbiology and Microbiome
OMICS, Comics and Metaproteomics:
Analyzing Microbiomes using proteomics
![Page 11: OMICS, Comics and Metaproteomics - Galaxy-Pgalaxyp.org/wp-content/uploads/2019/01/OmicComics_Pune_PJagta… · For example, Protein Database Downloader downloads ... Sci Rep. (2018)](https://reader035.fdocuments.us/reader035/viewer/2022070913/5fb47e2c6b7ef57a0828339a/html5/thumbnails/11.jpg)
Microbiome: Microbial genetic potential and response
Metagenomics: Identifies
species present within complex community (16S rRNA and Whole Genome Sequencing).
DNA from samples. 16S rRNA (economical) or
Shotgun sequencing (expensive).
Multiple studies that correlatetaxonomy with observed phenotype.
Multiple studies have shown correlation of microbial composition with physiological conditions. Also used to study interaction with environment.
tinyurl.com/medgadgetcom
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Morgan, Xochitl & Huttenhower, Curtis. (2012). Chapter 12: Human Microbiome Analysis. PLoS computational biology. 8. e1002808. 10.1371/journal.pcbi.1002808.
MICROBIOME RESEARCH
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Human Microbiome Project Consortium. Nature. 2012 Jun 13;486(7402):215-21. doi: 10.1038/nature11209.
microbial taxa vary while metabolic pathways
remain stable within a healthy population
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• Omics & Multiomics: Benefits and ChallengesMultiomics Research is emerging and will offer a more complete biological perspective. Multiomicsdata analysis presents challenges and opportunities.
• Functional microbiome analysesMultiomics approach in microbiome research has the potential to offer mechanistic insights.
• Metaproteomics, Galaxy and Projects
• Extending tools for metaproteomics
• Microbiology and Microbiome
OMICS, Comics and Metaproteomics:
Analyzing Microbiomes using proteomics
![Page 15: OMICS, Comics and Metaproteomics - Galaxy-Pgalaxyp.org/wp-content/uploads/2019/01/OmicComics_Pune_PJagta… · For example, Protein Database Downloader downloads ... Sci Rep. (2018)](https://reader035.fdocuments.us/reader035/viewer/2022070913/5fb47e2c6b7ef57a0828339a/html5/thumbnails/15.jpg)
• Omics & Multiomics: Benefits and ChallengesMultiomics Research is emerging and will offer a more complete biological perspective. Multiomicsdata analysis presents challenges and opportunities.
• Functional microbiome analysesMultiomics approach in microbiome research has the potential to offer mechanistic insights.
• Metaproteomics, Galaxy and Projects
• Extending tools for metaproteomics
• Microbiology and Microbiome
OMICS, Comics and Metaproteomics:
Analyzing Microbiomes using proteomics
![Page 16: OMICS, Comics and Metaproteomics - Galaxy-Pgalaxyp.org/wp-content/uploads/2019/01/OmicComics_Pune_PJagta… · For example, Protein Database Downloader downloads ... Sci Rep. (2018)](https://reader035.fdocuments.us/reader035/viewer/2022070913/5fb47e2c6b7ef57a0828339a/html5/thumbnails/16.jpg)
www.revitin.com
Microbiome: Microbial genetic potential and response
Multiple studies have shown correlation of microbial composition with physiological conditions.
Metagenomics: DNA Sequencing identifies species present within complex community (16S rRNA and Whole Genome Sequencing).
Metatranscriptomics: RNA Sequencing identifies species present and possible functions within complex communities (RNASeq).
Metaproteomics: The large-scale characterization of the entire protein complement of environmental microbiota at a given point in time. Potential to unravel the mechanistic details of microbial interactions with host / environment by analyzing the functional dynamics of the microbiome.
Metaproteomics approach goes beyond studies of compositional dynamics and sheds light on mechanistic details of microbial interactions with host / environment.
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Bond and Wilmes 2015
“Through the application of metaproteomics to different microbial consortia over the past decade, we have learnt much about key functional traits in the various environmental settings where they occur.” Proteomics. doi:10.1002/pmic.201500183.
Metaproteomics
Bond and Wilmes 2004
“The large-scale characterization of the entire protein complement of environmental microbiota at a given point in time” Environ. Microbiol. 6, 911–920.
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Metaproteomic Challenges
SINGLE-ORGANISM
PROTEOMICS
METAPROTEOMICS
SINGLE
SMALL TO MEDIUM SIZE (10 K TO
100K SEQUENCES)
SINGLE + CONTAMINANTS
SEARCH DATABASE- SIZE
- COMPLEXITY
LARGE (1 MILLION AND ABOVE)
MULTI-ORGANISM DATABASE WITH HOMOLOGOUS PROTEINS
• SEARCH ALGORITHMS BEING DEVELOPED TO ADDRESS LARGE AND COMPLEX
DATABASE SEARCHES
• PROTEIN GROUPING AT MULTI-ORGANISM LEVEL
• IDENTIFICATION STATISTICS AFFECTED BY LARGE DATABASES
• TAXONOMY BASED ON UNIQUE PEPTIDE IDENTIFICATIONS
• FUNCTIONAL ANALYSIS BASED ON PROTEINS IDENTIFIED
Disparate tools andmultiple processing
steps.
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Solution: Galaxy Platform
Goecks J et al Genome Biol. 2010;11(8):R86.
• A web-based bioinformatics data analysis platform.• Software accessibility and usability. • Share-ability of tools, workflows and histories. • Reproducibility and ability to test and compare results after using multiple parameters.• Ability to assimilate disparate software into integrated workflows.
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Solution: Galaxy Platform
For example, Protein Database Downloader downloads
UniProt protein FASTA databases of various organisms.
Software tools can be used in a sequential manner to generate analytical workflows that can be reused, shared and creatively modified.
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DATABASE
SEARCH
&
STRATEGIES
•
•
DATABASE
GENERATION
FASTQ
Protein / Peptide FASTA
TAXONOMY
ANALYSIS
Unique Peptides
FUNCTIONAL
ANALYSIS
Proteins
Known Function
Peptides
Search Algorithm
Spectra
Hypothetical Function
Unknown Function
Shared TaxonomyUnassigned Taxonomy
Metaproteomics Workflow
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Solution: Galaxy Platform
Metaproteomic analysis using the Galaxy framework.
Proteomics. (2015) doi:10.1002/pmic.201500074.
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• Plaque microcosms were grown from carious lesions in 12 children
• Extensive inter-individual variation in dental plaque composition
Metaproteomics Projects
Sucrose-induced dysbiosis in dental caries.
Rudney et al., BMC Microbiome DOI: 10.1186/s40168-015-0136-z
NS: Absence of sucrose WS: Presence of sucroseP: Plaque samples
• Paired design – microcosms grown in presence/absence of sucrose• Sucrose induces acid production in dental caries (dysbiosis)• 16S rRNA sucrose microcosms retained inter-individual taxonomic variation
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RAW data conversionDatabase generation
Database Search against HumanOral Microbiome Database (HOMD)
Convert microbial peptide lists to FASTA format
BLAST-P Analysis (30K to 60K peptides)
MEGAN Analysis
1 1
2
2
3
4
5
6
3
4
5
6
Metaproteomics Projects
Sucrose-induced dysbiosis in dental caries.
Rudney et al., BMC Microbiome DOI: 10.1186/s40168-015-0136-z
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SEED Functional Analysis
Metaproteomics Projects
Sucrose-induced dysbiosis in dental caries.
• 2-D LC-MS/MS Metaproteome was analyzed for taxonomy and function.
Rudney et al., BMC Microbiome DOI: 10.1186/s40168-015-0136-z
MEGAN5 Taxonomic Analysis
NS: Absence of sucroseWS: Presence of sucrose
• Taxonomically-diverse sucrose-pulsed microcosms clustered more closely by function. Function-based changes may be better indicators of dysbiosis
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Metaproteomics Projects
Clinical Projects
• MICROBIOME OF HUMAN CERVICAL-VAGINAL FLUID was analyzed
after collecting samples using residual Pap test fixatives from a pool of 40
normal samples and 5 individual women with normal cytology.
• Understanding the nature of the microorganisms and the functional role of
the expressed proteins is an important step to develop diagnostic tools for
gynecological conditions and malignancies.
MEGAN Analysis
1190 Microbial Peptides
30 genera. 42 species
Function: 939 peptides assigned to 137
InterPro2GO proteins
Skubitz Lab
MEGAN Analysis
776 Microbial Peptides
53 genera. 71 species
Function: 232 peptides assigned to 107
InterPro2GO proteins
• BRONCHOALVEOLAR LAVAGE FLUID (BALF)
MICROBIOME: Microbiome of fluid proximate to the site of lung
injury – acquired by using a bronchoscopy procedure – was analyzed.
• BALF from subjects with Acute Respiratory Disease (ARDS) patients
and lung injury after Hematopoietic Stem Cell Transplant (HSCT) were
analyzed.
Bhargava Lab
Jagtap P et al BAL Fluid Metaproteome in Acute Respiratory Failure. Am J Respir Cell Mol Biol. (2018) 59(5):648-652. doi: 10.1165/rcmb.2018-0068LE.
Afiuni-Zadeh S et al Evaluating the potential of residual Pap test fluid as a resource for the metaproteomic analysis of the cervical-vaginal microbiome. Sci Rep. (2018) 8(1):10868. doi: 10.1038/s41598-018-29092-4.
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SATELLITE Workshop @ ABRF Annual Meeting
March 25 2017San Diego, CA
• https://galaxyproteomics.github.io/abrf2017/
Noble Lab,University of WASeattle, WA
Workshop @ ASMS Annual
MeetingJune 7 2017
Indianapolis, IN
Workshop @ GCC Annual Meeting
June 28 2017Montpellier, France
• https://galaxyproteomics.github.io/asms2017/
• https://galaxyproteomics.github.io/gcc2017/
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Metaproteomics Gateway
Gateway on JetStream with documentation, tools & workflowsused at the ABRF workshop
z.umn.edu/metaproteomicsgateway
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• Omics & Multiomics: Benefits and ChallengesMultiomics Research is emerging and will offer a more complete biological perspective. Multiomicsdata analysis presents challenges and opportunities.
• Functional microbiome analysesMultiomics approach in microbiome research has the potential to offer mechanistic insights.
• Metaproteomics, Galaxy and ProjectsMetaproteomics workflows are available within Galaxy platform and are utilized workflows in research studies. The workflows can be accessed via public instance metaproteomics gateway.
• Extending tools for metaproteomics
• Microbiology and Microbiome
OMICS, Comics and Metaproteomics:
Analyzing Microbiomes using proteomics
![Page 30: OMICS, Comics and Metaproteomics - Galaxy-Pgalaxyp.org/wp-content/uploads/2019/01/OmicComics_Pune_PJagta… · For example, Protein Database Downloader downloads ... Sci Rep. (2018)](https://reader035.fdocuments.us/reader035/viewer/2022070913/5fb47e2c6b7ef57a0828339a/html5/thumbnails/30.jpg)
• Omics & Multiomics: Benefits and ChallengesMultiomics Research is emerging and will offer a more complete biological perspective. Multiomicsdata analysis presents challenges and opportunities.
• Functional microbiome analysesMultiomics approach in microbiome research has the potential to offer mechanistic insights.
• Metaproteomics, Galaxy and ProjectsMetaproteomics workflows are available within Galaxy platform and are utilized workflows in research studies. The workflows can be accessed via public instance metaproteomics gateway.
• Extending tools for metaproteomics
• Microbiology and Microbiome
OMICS, Comics and Metaproteomics:
Analyzing Microbiomes using proteomics
![Page 31: OMICS, Comics and Metaproteomics - Galaxy-Pgalaxyp.org/wp-content/uploads/2019/01/OmicComics_Pune_PJagta… · For example, Protein Database Downloader downloads ... Sci Rep. (2018)](https://reader035.fdocuments.us/reader035/viewer/2022070913/5fb47e2c6b7ef57a0828339a/html5/thumbnails/31.jpg)
DATABASE
SEARCH
&
STRATEGIES
•
•
DATABASE
GENERATION
FASTQ
Protein / Peptide FASTA
TAXONOMY
ANALYSIS
Unique Peptides
FUNCTIONAL
ANALYSIS
Proteins
Known Function
Peptides
Search Algorithm
Spectra
QUANTITATIVE
ANALYSIS
Spectral counts OR
Intensity data
Hypothetical Function
Unknown Function
Shared TaxonomyUnassigned Taxonomy
Metaproteomics Workflow
![Page 32: OMICS, Comics and Metaproteomics - Galaxy-Pgalaxyp.org/wp-content/uploads/2019/01/OmicComics_Pune_PJagta… · For example, Protein Database Downloader downloads ... Sci Rep. (2018)](https://reader035.fdocuments.us/reader035/viewer/2022070913/5fb47e2c6b7ef57a0828339a/html5/thumbnails/32.jpg)
metaQuantome: An integrated, quantitative metaproteomics approach reveals
connections between taxonomy and protein function in complex microbiomes
![Page 33: OMICS, Comics and Metaproteomics - Galaxy-Pgalaxyp.org/wp-content/uploads/2019/01/OmicComics_Pune_PJagta… · For example, Protein Database Downloader downloads ... Sci Rep. (2018)](https://reader035.fdocuments.us/reader035/viewer/2022070913/5fb47e2c6b7ef57a0828339a/html5/thumbnails/33.jpg)
metaQuantome: An integrated, quantitative metaproteomics approach reveals
connections between taxonomy and protein function in complex microbiomes
• Under the high-sucrose conditions, streptococcal sucrose
metabolism results in production of lactate, a stronger acid.
• Fusobacteria lack acid tolerance mechanisms, so they do not
survive well under a regimen of sucrose pulsing.
• Moreover streptococci can then compete more effectively with
fusobacteria for mucin oligosaccharides, which they are also
capable of metabolizing.
• Fusobacterium preferentially metabolizes amino acids.
• When mucin oligosaccharides are present, fusobacteria also can metabolize their
constituent sugars, such as galactose and mannose.
• However, most strains are unable to metabolize sucrose.
• Under those circumstances, the end products of carbohydrate fermentation in NS
conditions are weak acids.
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Integrated multi-omics of the human gut microbiome in a case study of familial type 1 diabetes.Heintz-Buschart et al NATURE MICROBIOLOGY 2, 16180 (2016) | DOI: 10.1038/nmicrobiol.2016.180
Microbiome Multi-omic Analysis
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FUNCTIONAL QUANTITATION
TAXONOMY-FUNCTION QUANTITATION
MetaPhlAn2
GraphPhlAn KRONA
Taxonomic abundance
Visualization of community structureGene Family
AbundancePathway
Abundance
GO Slim TermAbundance
HUMAnN2
Assembly Graph
Metabolic assignment
Predicted ORFs
Graph2Pep
Peptide Sequences
Protein Sequences
Peptides supported by MS/MS
Search
Graph2Pro
Protein DatabaseFor Metaproteomics
Search
Metatrancriptomics Data
Preprocessing(QC, trimming, dereplication, sorting)
Reads (FASTA)
FastQC, TrimGalore!, VSearch
SortmeRNA
Non-ribosomal RNA/DNA Reads
MEGAHITMetaSPAdes
Contigs
Taxonomy datafor UniPept
Mass spectrometry data
MAGs MaxBin
FragGeneScan
A workflow for integrated metatranscriptomic-metaproteomic informaticsASaiM
Graph2Pro
TAXONOMY QUANTITATION
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INTEGRATED MULTI-OMICS
ANALYSIS
Taxonomic Correlation Functional Correlation
Quantitative Correlation Visualization WP3
METATRANSCRIPTOMICS DATA
Quantitative Data
Taxonomic Abundance
Functional Abundance
Protein FASTA
METAPROTEOMICS DATA
Peptides
Taxonomy Analysis
Functional Analysis
Taxonomic Abundance
Functional Abundance
Binning Data
METAGENOMICS DATA
WP1 WP2
Quantitative Data Analysis
Protein FASTABinning Data
Multi‐omic informatics for characterizing microbiomes
and their role in health, disease and environment.
Norwegian Centennial Chair Program
![Page 37: OMICS, Comics and Metaproteomics - Galaxy-Pgalaxyp.org/wp-content/uploads/2019/01/OmicComics_Pune_PJagta… · For example, Protein Database Downloader downloads ... Sci Rep. (2018)](https://reader035.fdocuments.us/reader035/viewer/2022070913/5fb47e2c6b7ef57a0828339a/html5/thumbnails/37.jpg)
• Omics & Multiomics: Benefits and ChallengesMultiomics Research is emerging and will offer a more complete biological perspective. Multiomicsdata analysis presents challenges and opportunities.
• Functional microbiome analysesMultiomics approach in microbiome research has the potential to offer mechanistic insights.
• Metaproteomics, Galaxy and ProjectsMetaproteomics workflows are available within Galaxy platform and are utilized workflows in research studies. The workflows can be accessed via public instance metaproteomics gateway.
• Extending tools for metaproteomicsMetaproteomics workflows are getting extended to allow quantitative and multiomics analysis.
• Microbiology and Microbiome
OMICS, Comics and Metaproteomics:
Analyzing Microbiomes using proteomics
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• Omics & Multiomics: Benefits and ChallengesMultiomics Research is emerging and will offer a more complete biological perspective. Multiomicsdata analysis presents challenges and opportunities.
• Functional microbiome analysesMultiomics approach in microbiome research has the potential to offer mechanistic insights.
• Metaproteomics, Galaxy and ProjectsMetaproteomics workflows are available within Galaxy platform and are utilized workflows in research studies. The workflows can be accessed via public instance metaproteomics gateway.
• Extending tools for metaproteomicsMetaproteomics workflows are getting extended to allow quantitative and multiomics analysis.
• Microbiology and Microbiome
OMICS, Comics and Metaproteomics:
Analyzing Microbiomes using proteomics
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Microbiology and Microbiome
http://www.geograph.org.uk/photo/2815842
http://www.microbiome.at/sites/ami/files/images/Microbiome_1_CharisTsevis.jpeg
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Conclusions
• Multiomics Research is emerging and will offer a more complete biological perspective. Multiomics data analysis presents challenges and opportunities.
• Multiomics approach in microbiome research has the potential to offer mechanistic insights
• Metaproteomics workflows are available within Galaxy platform and are utilized workflows in research studies. The workflows can be accessed via public instance metaproteomics gateway.
• Metaproteomics workflows are getting extended to allow quantitative and multiomics analysis.
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Acknowledgements
Funding
galaxyp.org Follow us on: twitter.com/usegalaxyp
The Galaxy-P Team at University of Minnesota
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Minnesota Supercomputing InstituteJames JohnsonThomas McGowanMichael Milligan
Ira CookeMelbourne , Australia
University of Minnesota
Timothy GriffinPraveen KumarCandace GuerreroSubina MehtaAdrian Hegeman (Co-I)Art EschenlauerShane HublerRay SajulgaCaleb EasterlyAndrew Rajczewski
Biologists / collaboratorsLaurie ParkerJoel RudneyManeesh BhargavaAmy SkubitzChris WendtBrian CrookerSteven FriedenbergKevin VikenKristin BoylanMarnie PetersonSomiah AfiuniBrian SandriAlexa PragmanWanda WeberAmy Treeful
Harald Barsnes Marc Vaudel University of Bergen, Norway
University of Freiburg,Freiburg, Germany
VIB, UGhent, Belgium
Judson HerveyNaval Research InstituteWashington, D.C.
Matt ChambersNashville, TN
Alessandro TancaPorto Conte Ricerche, Italy
Carolin KolmederUniversity of Helsinki, Finland
Thilo MuthBernhard RenardRobert Koch Institut
Thomas DoakJeremy Fisher Indiana University
Josh EliasStanford University
Brook NunnU of Washington
Lennart Martens (Co-I)Bart MesuereRobbert G Singh
Bjoern GrueningBérénice Batut
Lloyd Smith (Co-I)Michael ShortreedUW-Madison
Karen ReddyMo HeydarianJohns Hopkins University
Anamika KrishanpalPriyabrata PanigrahiPersistent Systems Limited
Stephan KangIntero Life Sciences
galaxyp.org
FundingACKNOWLEDGMENTS
Magnus Øverlie Arntzen (Co-I)Francesco DeloguNMBU,Oslo, Norway