Old Title vs New Title NIH Guidelines for Research Involving Recombinant DNA Molecules NIH...

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Old Title vs New Title NIH Guidelines for Research Involving Recombinant DNA Molecules NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules Implement by 3/3/13

Transcript of Old Title vs New Title NIH Guidelines for Research Involving Recombinant DNA Molecules NIH...

Page 1: Old Title vs New Title  NIH Guidelines for Research Involving Recombinant DNA Molecules   NIH Guidelines for Research Involving Recombinant or Synthetic.

Old Title vs New Title

NIH Guidelines for Research Involving Recombinant DNA Molecules

NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules

Implement by 3/3/13

Page 2: Old Title vs New Title  NIH Guidelines for Research Involving Recombinant DNA Molecules   NIH Guidelines for Research Involving Recombinant or Synthetic.

Is chemical synthesis covered?

synthesizer

Not until in a …

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III-F Registrations Nothing exempt in Boston

BPHC adopts updated Guidelines unless…

Using nonhazardous plasmids (pBR) to clone gene of interest into host (nonpathogenic E.coli)

Often the first step in creating a transgenic animal

New exemptions for synthetic NA No origin of replication, no integration…

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NIH Guidelines: Definition of rNA

Molecules constructed by joining nucleic acids

& can replicate in a living cell

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NIH Guidelines: synthetic

Nucleic acids that can base pr w naturally occurring nucleic acids

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NIH Definitions Continued

Molecules resulting from replication of rNA or synthetic nucleic acids in past two slides

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Other Changes

Fewer AAV serotypes need review Transfer of drug resistance traits Human Gene Transfer w nucleic

acids

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Risk Assessment & Synthetics

% of genome from each parent Fn/purpose of each sequence

Assume same fn as original host? Synergism between sequences & transgenes

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What Next? - XNA

~6 sugars can form NA bbone

Store & retrieve genetic info

Medical benefit? Slower breakdown in stomach & bloodstream

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Real World Examples

iGEM 2006 Jay Keasling