Old Title vs New Title NIH Guidelines for Research Involving Recombinant DNA Molecules NIH...
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Old Title vs New Title
NIH Guidelines for Research Involving Recombinant DNA Molecules
NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules
Implement by 3/3/13
Is chemical synthesis covered?
synthesizer
Not until in a …
III-F Registrations Nothing exempt in Boston
BPHC adopts updated Guidelines unless…
Using nonhazardous plasmids (pBR) to clone gene of interest into host (nonpathogenic E.coli)
Often the first step in creating a transgenic animal
New exemptions for synthetic NA No origin of replication, no integration…
NIH Guidelines: Definition of rNA
Molecules constructed by joining nucleic acids
& can replicate in a living cell
NIH Guidelines: synthetic
Nucleic acids that can base pr w naturally occurring nucleic acids
NIH Definitions Continued
Molecules resulting from replication of rNA or synthetic nucleic acids in past two slides
Other Changes
Fewer AAV serotypes need review Transfer of drug resistance traits Human Gene Transfer w nucleic
acids
Risk Assessment & Synthetics
% of genome from each parent Fn/purpose of each sequence
Assume same fn as original host? Synergism between sequences & transgenes
What Next? - XNA
~6 sugars can form NA bbone
Store & retrieve genetic info
Medical benefit? Slower breakdown in stomach & bloodstream
Real World Examples
iGEM 2006 Jay Keasling