; - # _-......_...._,.. .. "'. -_ ,

, ~

Proceedings of .The 9th Joint Conference on Chemistry

Diponegoro University (UNDIP),

Semarang State University (UNNES), Sebelas Maret University (UNS) and

Jenderal Soedirman University (UNSOED)

Grand Candi Hotel, Semarang, 12-13 November, 2014

Green Chemistry

~itors

Owi Hudiyanti

Agustina LN. Aminin

Adi Oarmawan

Yayuk Astuti

UNNES Press

\ 2015

ISBN978-602-285-049-6Proceedings of

The 9'h Joint Conference on Chemistry

Cetakan ke 1

©2015 Chemistry Department, FSM, Diponegoro University

Judul Buku: Green Chemistry: Proceedings of The 9th Joint Conference on Chemistry

Editor: Owi Hudiyanti, Agustina L.N. Aminin, Adi Oarmawan, Yayuk Astuti

Penerbit: UNNESPress

ISBN:978..602-285-049-6

iii? (I g e Green Chemistry, Copyright

This Proceedings~Chemistry Department, FSM.Oiponegoro University 2015

Proceedings ofThe g" Joint Conference on Chemistry ISBN 978·602·285·049·6

Preface to The Conference Proceedings

We are very pleased to introduce The 9th Joint Conference on Chemistry (9th JCC) held byDiponegoro University (UNDIP) On behalf of the Chemistry Consortium In Central Java,Indonesia. The JCC Is an annual conference organized by the consortium of ChemistryDepartment of four universities in Central Java: Diponegoro University (UN DIP}, SemarangState University (UNNES), Sebelas Maret University (UNS) and Jenderal Soedlrman University(UNSOED); since 2006. The growing of environmental problems that persist to escalateworldwide has compelled us to select "Green Chemistry" as the leading theme of the 9th JCC.

We had 10 plenary speakers, 10 invited speakers and over 120 suitable papers from 11countries were submitted for presentation at the conference. This required the program to beorganized in five parallel sessions, each on a specific theme, to provide each paper withsufficient time for presentation and to accommodate all of them within the overall timeallocated. One of the five sessions contained analytical chemistry. A second session wasdevoted to the theme of biochemistry. The third and fourth session were dedicated to physicaland material chemistry. The fifth session was concerned with chemical education. These werewell represented in the program of the conference and were clearly topics which continue tostimulate a global interest. The programs were chaired in a professional and efficient way bythe session chairmen who were relected for their international standing in the subject.

All the papers went through a peer-review procedure prior to being accepted for publication Inthis book. These Proceedings present the permanent documentation of what was presented.They indicated the state of advancement at the time of writing of all aspects of this theme andwill be very useful to all people in the field.

As a final point, it is appropriate that we record our thanks to our fellow members of thesteering committee, organizing committee, and scientific committee. We are also indebted tothose who served as chairmen. Without their support, the conference could not have been tilesuccess that it was. We also would like to express our sincere gratitude to all authors for thel,valuable contributions -.We are thankful to the students of Chemistry Department Faculty ofScience and Mathematics Diponegoro University especially to Maya and Fuad for their supportduring preparation of the manuscript.

Dwi Hudiyantl

Agustina L.N. Amlnln

Adl Darmawan

Yayuk AstutJ

Green Chemistry, Preface

This Proceedings©Chemistry Department, FSM,Diponegoro University 2015

P,~se llil

Proceedings ofThe 9=" Joint Conference on Chemistry ISBN 978-602-285-049-6

Board of Reviewers

Prof. Joe da Costa, FIMLab - Films and Inorganic Membrane Laboratory, School ofChemical Engineering, The University of Queensland, Brisbane Qld 4072,Australia

Prof. Farook Adam, Universiti Sains Malaysia

Prof. Mohd Marsin Sanagi, Department of Chemistry, Faculty of Science, UniversitiTeknologi Malaysia, 81310 Johor 8ahru, Johor, Malaysia

David G. Churchill, Department of Chemistry, Korea Advanced Institute of Science andTechnology, South Korea

Owl Hudiyanti, Chemistry Department, Diponegoro University

Agustina L.N.Aminin, Chemistry Department, Oiponegoro University

Ad! Darmawan, Chemistry Department, Diponegoro University

Yayuk Astuti, Chemistry Department, Oiponegoro University

Khairul Anam, Chemistry Department, Diponegoro University

Parsaoran Siahaan, Chemistry Department, Diponegoro University

Bambang Cahyono, Chemistry Department, Diponegoro ~niversity

Melny Suzery, Chemistry Department, Diponegoro University

Eddy Heraldy, Chemistry Department, Sebelas Maret University

Fitrla Rahmawati, Chemistry Department, Sebelas Maret University

Maulidan Firdaus, Chemistry Department, Sebelas Maret University

Sayekti Wahyunlngsih, ~emistry Department, Sebelas Maret UniverSity

Sudarmin, Chemistry Department, Sebelas Maret University

Samuel Budi Wardhana Kusuma, Chemistry Department, Semarang St.te University

Uyi Sulaeman, Chemistry Department, Jenderal Soedirman University

Dadan Hermawan, Chemistry Department, JenderalSoedirman University

Green Chemistry, Board of Reviewers

This Proceedings©Chemistry Department, FSM,Diponegoro University 2015

Page Iv

I

j;

;..

-, '

.:

v :

"

',

"

•. ~ I

• I

Proceedings ofThe 9Uo Joint Conference on Chemistry ISBN 97S-602-28S.Q49-6

Table of Contents

Title Page

Copyright page

Preface

Board of Reviewers

Table of Contents

Section 1: Material Chemistry

nOz-SiO:zModified on Acrylic Paint with Self-Cleaning CharacteristicAgus Rldwan, Sri Wahyuni

Synthesis and Characterization of Cellulose Based Superabsorbent Polymer CompositesAhmad Zainal Abidin, N. M. T. P. Sastra, G. Susanto, H.P.R. Graha

Synthesis and Characterization of Nano Scale Zero-Valent Iron S.upported onMesoporous SilicaAtya/ Khalld Hammed, Nugroho Dewayanto, D. Dongyun, Mohd Rldzuan Nordin

Synthesis of 2, 7-Disulfonatonaphthalene-S-Hydroxy-4-Amino-N-Propyl Silica Hybrid bySol-Gel and Grafting ProcessesChoirll Azmiyawati, Nuryono, Narsito

Modification of Ni/Zn-HZSM-S Double Promoted Catalyst for Biofuel Production fromCerbera Manghas OilDanawdt; Hari Prajitno, Agus Budianto, Muhammad Iqbal, Achmat1 Roesyad~ VictorPurnomo

Influences of Ammonia for SyntheSis of 8-hydroxiquinoline Copper(lI)Suhartana, Laelatri Agustina, Sriatun

Influence of Variation Temperature on Phase Composition of Ca-Mg-AI HydrotalclteEddy Heraldy, Khoirina Dwl Nugrahaningtyas, Fendry Bangkit Sanjaya, Desl SuciHandayani, Yuniawan Hidayat

Synthesis and Characterization of Chitosan - Rice Husk Ash Silica Composite as PolymerElectrolyte Membrane (PEM)Eva Mardiningsih, Ella KU5umastuti

Synthesis and Characterization of the 2n(lI) Complex with Dimethyl Hydroxyl Pyrldine-2,6-DicarboxylateFahimah Martak

Green Chemistry, Table of Contents

This Proceedings@Chemistry Department, FSM,Olponegoro University 2015

II

Iii

v

vii

1

3

8

13

;

21

2S

29

34

38

44

'.

•

ISBN 978-602-285-049-6Proceedings of

The 9th Joint Conference on Chemistry

Synthesis and Characterization of lal.ySryCoo.aFeo.203and lal_yBayCoo.sFeo.Z03(O.O:!::ySO.4) . SODense MembranesHamzoh Fonsur~ N. Widiostun A. Aliyatu/muna, W. P. Utomo, O. Prasetyoko, B.Prljomboedi

Synthesis, Characterization and Catalytic Activity of CuO/ZnO on Phenol Oxidation 56Nuni Widiarti, Sri Wahyunl, 5 Barokah

Characterization of Enzyme Electrode from Nanochitosan Immobilized Glucose Oxidase 60on Carbon Paste Modified with Nanofiber Polyaniline for Biosensor ApplicationPop; Asr; Kumiatin, Loksmi Ambarsar~ Indo Setyawot;, Puspo Julistia Puspita, Aneisti

Andisol Soil Utilization of Mount lawu as Natural Adsorbent Multi Soil layering Materials 65for Domestic WastePranoto, R. 5udaryanto, Supriyadi

Modifying Surface Charge of Chltosan Membrane by Carboxymethylchitosan Blendedwith Poly(vinylalcohol)Retno Ar/adi tustano, Dwi Siswanta, Mudasir, T. Hayashita

Effect of N-doped Graphene for Pt/N-doped Graphene CatalystRikson Siburion, MinsyahriJ Bukit

The utility of Aqueous Extract of Air-dried CallophyJlum inophyllum L. leaf asMedium/Reduction System for Synthesis of Gold Nanoparticles (AuNPs)Sa/prima Yudha S., Zul/ikri Achid fIIardlia, Eka Angasa, Totok Eka Suharto, Yuta Nishina

The Impregnated Boron Oxide Catalysts for the Reaction of Dehydrogenation of EthaneSetiadi

Preparation of Zn-Ni/Ti02 Photocatalyst by Sol-gel Method and Its Activity in WaterDecompositionSigit Priatmako, E. Cahyono, S. Wahyunl, Ella Kusumastuti, Satria Bekti Uji Prambosto

Synthesis of Humic Acid Coated Fe304Magnetic Nanoparticle allP Its Application toAdsorp Cu(lI}Soerja Koesnarpadi, Daniel

Modification of Synthetic Zeolite from Bagasse Ash and Their CharacterizationSriatun, Taslimah, Linda Suyati

Synthesis and Surface Modification of nOz/Carbon Photocatalyst Produced by ArcDischarge In Ethanol MediumTeguh Endah Saraswati, Isya Fltr; Andhika, Astrid Olivia Nondiko, Sayekti Wahyuningsih,Candra Purnawana

The Effect of Vulcanization Time on Mechanical and Chemical Properties of liquidRubber CompoundTeja OwlSutantol Bambang Set;aj~ Kama Wljaya, Totok Eka Suharto

Calcium Phosphate-Chitosan Composite as a Bone Cement CandidateTr; Windarti o[1d Benjamin Horrocks ,

72

76

85

89

95

101

105

11()

114

119

viii IP age Green Chemistry, Table of Contents

This Proceedings©Chemistry Department, FSM. Diponegoro University 2015

Proceedings ofThe 9th Joint Conference on Chemistry ISBN 978-602-285-049-6

Optimization Processof H-Zeolite CatalystPreparationwith SurfaceResponseMethods 124Widayat, H. Susanto, H. Satriadl

Preparation of Activated Carbon from Oil PalmShell by Activating ZnChas Carbon 130Monoxide AdsorbentYuliusman, Widodo W. Purwanto, Yulianto S. Nugroho, Randy Anggriany

Section 2: PhysicalChemistry 135

Effectsof Voltage and Number of Cellon Desalinationof BrackishWater using 137ElectrodialysisMethodAlfan Purnomo, Zakiatul Mlrjada, Arseto Yekti Bagastyo

Mesostructured Titanoslllcates Catalyst for Synthesisof Vitamin K3 142Alfa AkustiaWidati, Hamami, Handoko Darmokoesomo, Noda Adhisty Stevany

XRDof SynthetiCZeolite for Surfactant Builder: NaOHConcentration Variation in Sodium 146Silicate Decisionof RiceHuskAshArnelli, Ahmad Suseno, Teguh Imam Prasetyo

Catalytic Conversionof 1-0ctadecene to Shorten ChainsAlkane (t.;- Cll) 149D. Setyawan Purwo H, Triyono, Norsito, Tutik Dwi Wahyuni

ElectrochemicalCharacterization of Direct EthanolFuelCell (DEFC)with Crude 153Bioethanol FeedDwl Kemala Putrl, Mitra Elliani, Aditya Yudistira, Isd/riayani M. Nurdin, Hary Devlanto,Ardiyan Harimawan

Conversionof ~Iycerol into Polyhydroxybutyrate(PHB)uSingEscherichla coliEndah Fitriani Rahayu, Wega Trisunaryantl, Kama Wljaya

156 ;

The Effectsof HydrolysisTemperature and CatalystConcentration on Bio-ethanol 161Production from BananaWeevilEni Budiyatl and Umar Bondi

The Effectof Coconut 011Concentration on Physicaland ChemicalProperties of Cosmetic 167EmulsionsEn! Widiyat~ AH. Bambang Setla)l, Totok Eka Suharto, Trlyono

Adsorption of Pb(II) and CollI) on Adsorbent ClayImmobilized Saccharomyces cerevlsiae 171BiomassFahmlati, Mashunl, L.D. Syahdam HamIdi, Nasta

Utilization of CassavaPeelas Electric EnergySourcethrough Microbial Fuel Cell 178Linda Suyati, Dldik Setiyo Widodo, Abdul Haris, Wuryontl, Rahmod Nuryanto

Effect of Activated BagasseCharcoalSizeas Biomaterial Pretreatment on Waste Cooking 181Oil BlodieselCharacteristicsLizda lobar Mawaranl, Tatlk Farlhah

Green Chemistry, Table of Contents

This ProceedingsCi:)Chemistry Department. FSM, Diponegoro University 2015

p .. g e Ilx

ISBN978-602-285-049-6Proceedings of

The 9'hJoint Conference on Chemistry

Electrochemical Characterization of Direct Ethanol Fuel Cell (DEFC)with Bioethanol Feed 185Containing Acetic Acid as ImpurityMitra Evian~ Isdiriayanl M. Nurdln, Hary Devianto

The In Silleo Molecular Interaction of Organoboron Compounds as Curative Measure 189toward Cervical CancerRidla Bakrl, Arli Ad/tya Parikesit, Clpta Priyo Satrianto, Djat; Keram~ Usman Sumo FriendTambunan

Catalytic Properties of Bimetallic NiNP-M/ AIOH (M = Sn, In, Ga, Ag, Nb, and Zr) onSelective Hydrogenation of FurfuralRodiansono, M. D. Astut~ A. Gho/ur, Shogo Shimazu

Adsorption Study of 2-mercaptobenzothiazole at Copper Surface as Corrosion InhibitorinHCITaumy Alif Firman, Yokl Yullzar

Emulsification Ability of Surfactant-like Peptides Predict~d by Coarse Grained MolecularDynamics SimulationsTegar Nurwahyu Wijaya, Rukman Hertadl ".

Biofuel from Light Tar Resulted from Coconut Shell Pyrolysis by Distillation ProcessUswatun Hasanoh, Bambang Setla}l, tnyono, Cha/ril Anwar

Adsorption of Cyanide Ion from Aqueous Solutions by Saccharomyces cerevisiae BiomassVenty Suryant~ Fltr/a Rahmawatl, Yudha Anggara Haeqal

Biosorptlon of Cu2+, Zn2+, and Cd2+ by Nannochloropsis salina in a Three-Metal SystemYusa/ir Hala, Emma Suryati, Paulina Taba, Nesty MudlTumale

The Effect of Annealing Temperature to The X-Ray Diffraction Patterns of The Thin Filmof Cardanol Compound from Alor Regency NTI ProvinceZakarios Seba Ngoro, J Gusti M. Budlano, Aliwarsito

Section 3: Analytical Chemistry

Effect of pH on Cu-STIOz Photocatalytic Performance toward Phenol Photodegradationand Cr(VI) Photoreduction by Visible LIght IrradiationAbdul Haris, Didik Setiyo Widodo, Rohmad Nf.!ryonto

Electrochemical Impedance Spectroscopy AnalysiS of lithium Polymer Batteries duringCharge/Discharge CycleAehmad Roehliadl, Multozam, I Made Arcana, Bunbun Bund}ali

Influence of C/N Ratio in Activated Sludge to Remove Cr(VI)Arseto Yekt/Bagastyo, Natalia Dlanl Triana

·Method Development and Validation for Lead (Pb) AnalYSisin Natural Honey from EastKalimantanBohar; Yusuj, Finqo Aprianto . ,

xlFage Green Chemistry, Table of Contents

This proceedings@Chemistry Department, FSM, Diponegoro University 2015

193

199

202

20S

209

213

217

221

223

226

230

238

Proceedings 0/The 9tlt Joint Conference on Chemistry ISBN 978-602-285-049-6

Electroremediation of Polluted Water: Electrodecolorization of Batik Wastewater 243Didik Setiyo Widodo, Abdul Haris, Gunawan

Influence on The Degreeof Increasein Natrium Metabisulphlte White Bread Flour 248Heny Kusumayanti, Laila Faizah, R.TD. Wisnu Broto, Hani/ah, M. Taqiyuddin

SelectiveAdsorption of Phenol and Vanillin UsingEugenolBasedMolecularly Imprinted 251PolymerM. Cholid Djunaid~ Dwi Siswanta, lumina

"The Influence of AscorbicAcid, Creatinine and Urea on the Analysisof Uric Acid In the 258Blood Serumby Stripping Voltammetry usingGraphite ElectrodeMiratul Khasanah, Handoko Darmokusumo, Ganden Supriyanto, Ahmad Zaky PUlungan,Putut Satrio Dahono

Optimization and Validation of HPLCfor Analysisof RhodamineB In SpongeCake 263Novi Yantih, ZuhelmiAziz, Ac(itya Dicky Prasetya

Analysisof 8 Human Pharmaceuticals in Water SamplesUsingSolid PhaseExtraction 267Followed by Liquid Chromatography TandemMassSpectrometrySamuel Bud; Wardhana Kusuma, Ibrahim AI Tarawneh, Robert '(reuzig

Analysisof Nitrosodiethylamine (NDEA)in SaltedFishwith Hollow Fibre-liquid Phase 273Microextraction GasChromatography FlameIonization Detector(HF-lPME-GC-FID)MethodUsreg Sri Handajan~ Ganden Supriyanto, Yanuardi Raharjo, Gunawan Dwi Saputra

Application of ConeShapedMembrane-Liquid PhaseMicroextractlon for Analysis 278Nitrosodipropylamine in Salted FishYanuardi Raharjo, Usreg Sri Handajani, Eko Aryo Wijaksono

Section 4: Organic Chemistry 283

PhytochemicalScreeningand Toxicity Test BSlTfor Ethanol Extract of 285Agarwood(Aquilaria microcapa Balli)Ahmad Mus/r, Rlsma M. Tambunan, BambangTriseno

Determination of Glabridin in licorice Root (Glycyrrhiza glabra L.) UsingHigh 289Performance liquid ChromatographyFarldah, Siti Umrah Noor, Rahmawati 1.

Antidiabetic and Antlhypercholesterolemlc Activities of Citrus SinensisPeel In Rats 294Haryoto, Muhtadi, Tanti Azizah, And/ Suhendl

Acute Toxicity for Combination Extract of Terminalia muelleri Benth. and Curcuma 298xanthorrhlzaKhairul Anam, Dewl Kusrini, Ratna Megawati Wldharna

The Effect of Oil Typeson The Characteristicsof SolidSoapMardiyah Kurniasih, Purwati, Anung Riapanitra, Zus!ahair, Tri Wahyuni

303

-,

Green Chemi5try, Table of Contents

This Proceedings©Chemistry Department, FSM, Diponegoro University 2015

Pagt! lid

978-602 -285-049-6Proceedings of

The 9th Joint Conference on Chemistry

Antibacterial Activities SomeCompoundsCloveleaf Oil Derivatives 308Ngadiwiyana, Purbowatiningrum Ria Sarjono, Enny Fachriyah, Nor Basid AdiwibawaPrasetya

The Effectof the Addition of Glycerol and ChitosanIn the BiodegradablePlastics 312Production from "Porang" Flour (Amorphophallus mueller/Blueme)Ninlek Fajar Pusplta, Saidah Altway, Lizda Johar Mawaranl, Dwl Ayu, Dessy Rosita

Standardizationand a-GlucosidaseInhibitory of Extract from Anredera Cordifolia Leaves 317Ratna Djamil, Wlwl Wlnartl, Syamsudln, Merrysca Rasna

Determination of Total Flavonoid Content andStandardization Orthosiphon or/status 322LeavesExtractsSarah Zaldani Ratna Dlamll

Effectof ReactionTime toward Formation of 1,S-Bis-(2-Furanyl)-I,4-Pentadien-3-0ne 326from Claisen-Schmidtcondensauon of Furfural andAcetoneSit/ Mar/yah UI/a, Indah NurPramestl, M. Farld Rahman, Hldeki Okamoto

BloodChemistryData Baseof KeduChicken;-TheIndonesian IndigenousPoultry 330Siti Susanti, Rina Muryani, Isroll, Hanny Indrat Wahyunl, Agus Suelpto

The Potencyof Liquorice Extract (Glycyrrhlza glabra L) asSkinWhitening 334Siti Umrah Noor, Faridah, Astri Windi

Trlterpenoids from Tembelekan(Lantanacamara) LeafExtractand Its Activity asan 339Antibacterial (Escherichia coli)Sitti Hadijah Sabarwat~ Oce Astuti, Indriyani Nur

Hydrothermal Methods for HydrolysisCelluloseto Glucoseand/or Oligosaccharide:A 341ComparativeStudywith and without Ultrasound PretreatmentSumari, A. Roesyadl, Sumarno

ChemicalConsJituentof oeM Extract and Neutral-Acid Fraction of Voacangafoetida (BI.) 345Rol/e leaves from Three Locationsof Lombok Islandon The Basisof GC-MSAnalysisSurya Hadi, Lely Kurniawan Baiq Mariana, Handa Muliasar~ Sri Rahayu

Preparation and Characterization of Inclusion Complexof Xanthone with 351Suffonatocallx[4}areneTriana Kusumaningsih, Maulidan Firdaus, Muhammad Widyo Wartono, Desi SuelHandayan~ Sidlq Nugraha, Tegar Parnandi Galih Rasdian

Quality Standardizationand Determination of InVitro Antihiperglicemic Activity of 355EthanollcExtractof PacarKuku (Law50nia inermis Linn.)Wiwl Winart~ Syamsudin, Ratna Djamll, Aloysius Sebastian

PhenolicCompoundsfrom the leaves of Kalanehoe blossfeldiana (Cra5sulaceae) Plant 359Yenny Febrfani Yun, LiI/sSiti Aisyah, Tri Reksa Saputra, Arif Rohman Hakim, Tatf Herlina,EuisJulaeha, Achmad Zainuddin, Unang Supratman

BioactiveComponentsand Antioxidant Properties of SteviaBeverage 363Yohanes.Martono, Hartati Soetjipto

xiilPage Green Chemistry, Table of Contents

This Proceedings@Chemlstry Department. FSM. Diponegoro University 2015

Proceedings 0/The s" Joint Conference on Chemistry ISBN 978-602-285.049-6

Characteristic of 70% Ethanol Extract'from Cyclea barbata Miers leaves and Antioxidant 369Activity using DPPH MethodYunahara Farida, Erlindha Gangga, Kartinfngslh, Elisa, Teguh

Section 5: BiochemIstry 375

Isolation and Partial Purification of New Protease form Thermophilic Bacteria 377Pseudomonas atitidis WN 1 obtained from Indonesian Hot SpringAmln Fatoni, Zus/ahair

The Complexity of Molecular Interactions and Bindings between Cyclic Peptide and 38lInhibit Polymerase A and B1 (PAC-PBlN) HlNl ."Arli Aditya Parlkesit, Harry Novlard~ Djoti Keraml, Usman Sumo Friend Tambunan

Identification and Characterisation of Bioactive Peptldes of Fermented Goat Milk 386Chanl/ Mahdl, H. Untar~ M. 'Padogo

Comparative of Biomass for Pretreatment with Biological Process for Efficient Hydrolysis 391Desy Kurniawat~ Muhamad Natslr, Rahmi Febrialis, Prima Endang Susllowatl

Characterization of Immobilized lipase from Fractionation Result of Azospirillum Sp. 391Prdl using ChitosanDian Riana Ningslh, Zus/ahair, Santi Nur Handayan~ Pujl Lestari

Hydrolysis Enzyme Production a-Amylase and p-Glucosldase from Aspergillus niger with 402Solid State Fermentation Method on Rice Husk, Bagasseand Corn Cob SubstrateHeri Hermansyah, Rizky Ramadhan~ Adinda Putri Wisman, Rita Arb/anti

Alkaline protease activity of Black Aspergilli isolated from soil of West Sukolilo Madura 408Isworo Rukmi, Wuryantl, Arlno Trl Lunggani•Anti Hyperuricemia Activity of Salam (Syzigium Polyanthum Walp.) and Meniran 413(Phyllanthus niruri Linn.) Herbs Extracts in Oxonate-Induced Mice .Muhtad~ Andi Suhend~ Nurcahyanti W., EM. Sutr/sna

lignocellulolytic Enzyme Complex of Thermophilic Compost for Agriculture Biomass 420ConversionNies Sucl Mulyanl, Octa/sari K. Saputr~ Agustlna L.N. Amlnln

Biogas from the Solid Waste of Dairy Cattle as Renewable Alternative Energy at Mowifa 424" and Konda, Konawe Selatan, Sulawesi Tenggara .

Prima Endang Susilawatl, Ahmad Zaen/, Darwis

Production and Characterization of Blosurfactant from Halophilic Bacteria Pseudomonas 429stutzeri Strain BK-AB12Rukman Hertadl, Desyka Sari Siholoho, Deana Wahyunlgrum

The Ability of Bacterial Isolates of Actinobacillus sp. in Degrading Pollutants p-Cresols 434and Sunset YellowSubondi, Muntholib, Eli Hendrlk Sanjaya, Prita Olivia Putri

-,

Green Chemistry, Table of Contents

This Proceedings@Chemlstrv Department, FSM, Diponegoro Unlversltv 2015

Page I xIII

ISBN978-602-285-049-6Proceedings of

The 9th Joint Conference on· Chemistry

Section 6: Chemical Education 439

The Model Development of ChemicalPracticalWork Approach and Performance 441Assessmentto Increasethe Performance of the Laboratory PractitionersEndang Susilaningsih, Murbangun Nuswowatl

Measure Student Teachers'Ability to Implement Authentic Assessment 448Harjito, Sri Nurhayati

Chemical-ScienceEducation Integrated with Religion 454Kasmadi Imam Supardl

GivingTaskDesigningand Presenting Environmental Problem Solvingthrough 461Environmental Chemistry Courseto IncreaseCharacterValuesand Knowledge of theStudents .Murbangun Nuswowatl

The Application of Discoverylearning with ScientificApproach to Improve the Students' 466ScienceProcessSkillNalla Ayodiya, Woro Sumornl

Science,Environment, Technology and Society (SETS)Oriented Mini-Chern Book 470Nor Horisoh, WoroSumarni

Inquiry Learning in Laboratory by HPLCReversed-PhaseMethod Development in Taking 476the Conditions of HeavyMetals SeparationSrlWardanl

xlv] rag e Green Chemistry, Table of Contents

This Proceedings~Chemistry Department, FSM,Diponegoro University 2015

Proceedings ofThe 9th Joint Conference on Chemistry

Proceedings of The 9th Joint Conference on Chemistry

Diponegoro University

Green Chemistry Section 4:

Organic Chemistry

Green Chemistry Section 4: Organic Chemistry

This Proceedlngs©Chemistry Department, FSM, Dlponegoro UniverSity 2015

ISBN978-602-285-049-6

P age 1283

Proceedings 0/The!f' Joint Conference on Chemistry ISBN 97S-602-285.Q49-6

Phenolic Compounds from the Leaves of Kalanchoebloss!eldlana(Crassulaceae) Plant

VenRYFebriani Yuna,b,.,lUis Sitl Aisyah''', Trl ReksaSaputra', Arif Rahman Hakim', Tatl Herflna,Euls J~laeha', Achmad Zalnuddfn', Unang Supratmanl

I, Abstract

Kalanchoe bloss/eldlana(Crassulaceae} is a succulent plant belonging to the genus Kalanchoe.Various species 9f Kalanchoe ·plants have been widely used as raw material for traditionalmedicine and also as an ornamental plant. This study Is a continuation searching for secondarymetabolites from Kalanchoe Indonesia plants. Fresh K.bloss/eldfana leaves 5.7 kg wasextracted with methanolat room temperature to obtain a concentrated extract as much as155.74 g. Concentrated methanol extract was dissolved in water and then partitionedsuccessively within-hexane and ethylacetate. Ethylacetate extract (20 g) was separated by acombination of chromato.,-aphy on silica and ODS and produced compound 1 (7.1 mB) In theform of a yellow solid, compound 2 (4 mg), and compound 3 (15.4mg) In the form of a whitesolid. The chemical structures of compounds 1, 2, and 3, were determined by UV spectroscopy,IR, MS, 1H-NMR, 13C-NMR spectroscopy and comparison of data obtained from the literatureand Identified as phenolic compounds, namely 3,3',4',5,7 pentahydroksl flavonorquercetin (1),epigallocatechlngallate (2), and gallic acid (3).

Keywords:Kalanchoe bloss/eldlana,eplgallocatechlngallate, gallic acid

phenolic, 3,3',4',5,7 pentahydroksi f1avon,

•NatunJlProduct Chemistry Group,Department of Chemistry,TIleFaculty of Mathematla and Natural Sciences,PadJacfJaranUnM!tslty,Bandung.5umedanl Km21,Jatinanlor,sumedang 45363

'Department of Chemistry,The Faculty of Mathematfa and NaturalScIences. Jenderal Adlroad YanlUniversity,Clmahl40528

Introduction

Corresponding author emaUaddresses: • yennyfy@gmail.com,··o supratman@unpad.ac,!d

Indonesia has hIgh plant biodiversity and itsecosystems (WaluJo, 2011).50me of the plants mostlyused by IndonesIan society, especially as herbalmedicines, are from the genus Kalanchae.Usually theyare used as ornamental plants, with thecharacteristics of thIck and watery leaves. Theseplants are very popular because of easypropagatlon,low water needer, and the flowercolours. from the point of ethnopharmacologyKa/anchae plants are used as traditional medicines tocure headache, coup, chest pain, ulcer. and othersldn deseases. They overcome fever, fix the Irregularmenstrvatlon, heal wound and boD, not only inIndonesia but also almost everywhere In the world(Quazl et al, 2011). Some researches reported thatKa/anchae plants contain bufadlenollde (Supratmanet al, 2000; Supratman et a••, 2001). triterpenold(Galnd and Gupta, 1972), and flavonoid (Muzltano etal., 2006; OkWu and Nnamdl, 2011), and biologicalactivities like antilelsmanlal, antllnffamatory,cytotokslt. and InhIbiting tumor ceU growth

(Supratman et al.. 2001; Costa, 2006; Biswas et al.,2011).

This study Is a continuation searching for secondarymetabolites from Kalanchoe Indonesia planb~Specially Kglanchoe bloss!eldlano(Crassulaceae).

Methodology

Materials

Plant

The leaves of K. bloss/eldiana were collected fromLembang, Western Bandung area, West Java,IndoneSia, and Identified at Herbarium BOBoriense,Biology Research Centre of lIPI Lembaga /ImuPengetahuan Indonesia or L1PI (The IndonesianInstitute of ScIences), Cibinons. BOlar, West Java,Indonesia.

Chemical

The chemicals needed were both the varfoustechnical solvents (redistilled), i.e.n·hexane, ethyl

Green Chemistry Section 4: Organic Chemistry, Venny Febrlanl Yun. et al.

This Proteedings@Chemlstry Department, FSM, Oiponegoro University 2015

Page 1359