OEB 192 – 11.10.17 Dynamics of adaptation. (Miki Lee, Nigel Delaney, Maryska Kaczmarek, Lewis...
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OEB 192 – 11.10.1 Dynamics of adaptation
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Transcript of OEB 192 – 11.10.17 Dynamics of adaptation. (Miki Lee, Nigel Delaney, Maryska Kaczmarek, Lewis...
OEB 192 – 11.10.17
Dynamics of adaptation
(Miki Lee, Nigel Delaney, Maryska Kaczmarek, Lewis Ward)
In humidified, 30 °C room:48-well plates
Plate-shaking tower (x38)De-lidding station
Twister armPlate reader
Custom software (system, data)
Growth analyzed via automated, robotic system
(Delaney et al., in prep)
OD
600
ln(O
D60
0)
6 replicates…
tD = 3.449 h95% ci = ±0.006 h
R2 > 0.999
Fitness assayed via competitions
(Lee et al., 2009. Evolution)
Evolved Fluor. ancestor
T0
Competition Flow Cytometry
Competitive growth
T1 T1 fluorescence
Evolved Fluor. ancestor
T0 fluorescence
W = (ln(E1/E0)) / (ln(A1/A0)) (David Chou)
Rapid improvement in 600 generations
(Chou et al., 2011. Science; Chubiz et al., In prep)
2. Mainly M-specific
3. Fitness ≈ growth rate
1. Fitness gains large but varied
8 populations & average
Population 4
Isolate to sequence
Population 4
Why such a range?
(Paquin & Adams, 1983)
Wednesday (10/19):
Tradeoffs, specialization & pleiotropy
