Objective Computer Assisted Review of DNA Evidence Dan E. Krane Wright State University, Dayton, OH...
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Objective Computer Assisted Review of DNA Evidence
Dan E. KraneWright State University, Dayton, OH 45435
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The business of DNA profiling:
Roughly 900,000 felony convictions per year in US
DNA profiles generated primarily for sexual offenses, murder and assaults.
Combined DNA Index System (CODIS)
Less than 1% of DNA profiles reviewed by defense experts
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DNA profiling approaches:
VNTRs
PCR/reverse dot blots
STRs
Advantages Disadvantagesresolving power, inexpensive
testing time, sensitivity, data comparisons
sensitivity, testing time, data comparisons
resolving power, mixtures
very sensitive, testing time, data comparisons, resolving power
start-up costs
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DNA contents of biological samples:Type of sample Amount of DNA
Hair:
Blood 30,000 ng/mLstain 1 cm in area 200 ngstain 1 mm in area 2 ng
Semen 250,000 ng/mLpostcoital vaginal swab 0 - 3,000 ng
plucked 1 - 750 ng/hairshed 1 - 12 ng/hair
Saliva 5,000 ng/mLUrine 1 - 20 ng/mL
2
2
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Allele 2
Allele 7
Allele 12
Allele 13
Allele 17
Primer bindingsite
Tandemly repeated
sequences
Amplified region
Short Tandem Repeats (STRs)
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What you see when you open a CD provided in discovery:
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What you see when you try to open the files on a discovery CD:
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An electropherogram using the software’s default settings:
GenoTyper Graph
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What the testing lab provides is not always the same as the default electropherogram:
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Genophiler Zoom
• Genophiler also provides a zoomed graph to 150 RFU’s• Shows smaller peaks more clearly• Here, the peaks that were disregarded by the lab may be evidence of an unknown, minor contributor to the sample
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No mention of a potential, secondary contributor to the sample.
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Still no mention of a potential, secondary contributor to the sock sample.
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Victim Run:Sample Nickname1:F011542 03P.fsa ALLEGED VICTIM OL (99) 14 (237) 15.2 (309)1:F011542 04A P.fsa SUSPECT STAIN CARD 15 (1120) 17 (1351)1:F011542 02E1P-2.fsa TOWEL CUTTING (Non-sperm Fraction) 15 (1611) 17 (1255)1:F011542 02E2P-2.fsa TOWEL CUTTING (Sperm Fraction) 15 (868) 17 (802)1:F011542 02E2 Pr.fsa TOWEL CUTTING (Non-sperm Fraction) 15 (1740) 17 (1644)1:F011542 01P.fsa SOCK CUTTING OL (125) 14 (2622) 15.2 (2189) 19 (167) OL (140)1:F011542 04 raP-2.fsa 15 (278) 17 (167)
D3
Genophiler output:
Testing lab’s report:
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16 (253) 20 (184) 12 (368) 13 (350) 9 (285) 12 (281) 10 (359)14 (1130) 19 (949) 12 (1626) 13 (1565) 8 (1561) 12 (1148) 10 (895) 11 (944)14 (1495) 19 (1223) 12 (2932) 13 (2703) 8 (2423) 12 (2161) 10 (1267) 11 (1078)14 (1026) 10 (114) 12 (1272) 13 (1052) 8 (1070) 12 (838) 10 (760) 11 (709)14 (1755) 19 (1531) 12 (1874) 13 (1617) 8 (1652) 12 (1328) 10 (1250) 11 (1178)9.2 (73) 16 (628) 20 (483) 12 (2907) 12 (926) 10 (1187)14 (135) 19 (97) 12 (158) 13 (122)
D18 D5 D13 D7
15 (242) 16 (264) 21 (300) 22 (269) X (529) 12 (272) 13 (280) 27 (277) 28 (269)18 (1216) 19 (814) 21 (1352) 22 (1271) X (1347) Y (1288) 15 (2554) 29 (2687)18 (1128) 19 (899) 21 (1906) 22 (1837) X (1907) Y (1756) 15 (3885) 16 (70) 29 (4458)18 (767) 19 (564) 21 (858) 22 (541) X (1191) Y (1187) 13 (81) 15 (2242) 16 (97) 29 (2027)18 (1595) 19 (1166) 21 (1817) 22 (1154) X (1842) Y (1858) 15 (3364) 29 (3355)15 (1290) 16 (1144) 21 (1430) 22 (1394) X (5015) 12 (3418) 13 (2876) 27 (1020) 28 (1032) 30 (72)18 (153) 19 (190) 21 (230) X (196) Y (287) 15 (403) 29 (352)
D21D8vWA FGA Amel
Victim Run:Sample Nickname1:F011542 03P.fsa ALLEGED VICTIM OL (99) 14 (237) 15.2 (309)1:F011542 04A P.fsa SUSPECT STAIN CARD 15 (1120) 17 (1351)1:F011542 02E1P-2.fsa TOWEL CUTTING (Non-sperm Fraction) 15 (1611) 17 (1255)1:F011542 02E2P-2.fsa TOWEL CUTTING (Sperm Fraction) 15 (868) 17 (802)1:F011542 02E2 Pr.fsa TOWEL CUTTING (Non-sperm Fraction) 15 (1740) 17 (1644)1:F011542 01P.fsa SOCK CUTTING OL (125) 14 (2622) 15.2 (2189) 19 (167) OL (140)1:F011542 04 raP-2.fsa 15 (278) 17 (167)
D3
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Mix X/Y Deg. R Low (<150) Pull Up Nickname Run:SampleM1 -0.0206 1 0 ALLEGED VICTIM 1:F011542 03P.fsa
1.05 -0.0153 0 0 SUSPECT STAIN CARD 1:F011542 04A P.fsa1.09 -0.0528 1 0 TOWEL CUTTING (Non-sperm Fraction) 1:F011542 02E1P-2.fsa
M2 1.00 -0.0221 3 0 TOWEL CUTTING (Sperm Fraction) 1:F011542 02E2P-2.fsa0.99 -0.0101 0 0 TOWEL CUTTING (Non-sperm Fraction) 1:F011542 02E2 Pr.fsa
M3 -0.0823 4 0 SOCK CUTTING 1:F011542 01P.fsa0.68 -0.0724 3 0 1:F011542 04 raP-2.fsa
Genophiler also flags potential problems for further review:
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Project Screen Button
• Screen shot of GeneScan
• Shows exactly what samples were added and what parameter files were used for the analysis
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Analysis Parameters Buttons
• See exactly what analysis settings were used
• Parameter files also copied to CD
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Finding Problems With Samples: Raw Data
Good raw data begins with a large peak followed by smaller peaks
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Finding Problems With Samples: Raw Data
• Bad raw data is indicated by wildly uneven peaks• Indicates lab analysis problems
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Finding Problems With Samples: EPT Data
• Shows current, voltage, laser power, and temperature• For good analysis, should be constant (flat)
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Finding Problems With Samples: EPT DataFinding Problems With Samples: EPT Data
• Problem EPT data is not flat• Indicates problems with lab analysis
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Automation Process
• Select samples and settings in one screen
• Analysis can be performed unattended
• Up to 4 analysis runs can be set up at one time
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Forensic BioInformatic Services• Uses Genophiler to generate easily interpreted files
• Objectively applies analysis parameters to all samples
• Fast turn around times
• Efficiently draws attention to problems requiring further review
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Acknowledgements:
Genophiler programmers: Dr. Travis Doom Dr. Mike Raymer Dr. Oscar Garcia Jason R. Gilder
STR experts: Dr. Bill Thompson Dr. Simon Ford Dr. Dan Krane
Support:Wright State University, Dayton, OH
Forensic BioInformatic Services; Suite 150, 2850 Presidential Drive, Fairborn, OH, 4538x; phone: (937) xxx-xxxx; web site: BioForensics.com