O32 Coffey
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Past and Current Taxonomic Statusof Phytophthora cryptogea and P. drechsleri
and Associated SpeciesA Work in Progress
Michael D. Coffey , Shirley Tu & Masoomeh Peiman
University of California, Riverside
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After alignment of the edited sequences with ClustalW and MacClade, phylogenetic analyses were conducted in MEGA 4 (1), Neighbor-Joining method (2)the percentage of replicate tree in which the associated
taxa clustered together in the bootstrap test (1000 replicates) are shown next to branches (3)
• 1. Tamura K, Dudley J, Nei M & Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24:1596-1599.
• 2. Saitou N & Nei M (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406-425.
• 3. Felsenstein J (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783 – 791.
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242 accessions used to generate ITS sequences
See the 2,5 meter ITS Tree!!!
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48-ITS
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LSU
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COXI barcoding• Cytochrome Oxidase I (COXI)• Good primers designed that amplify a 727bp region of COXI• No introns in oomycete COXI (as opposed to true fungi)• From G.P. Robideau, A.W.A.M. de Cock, M. Peiman, K. Bala, M. D. Coffey, and C.A. Lévesque. 2008
78 778900
12600
Barcode
<-FM85-modOom-COI-Lev-up->
Martin, F. N., and P. W. Tooley. 2003. Phylogenetic relationships among Phytophthora species inferred from sequence analysis of mitochondrially encoded cytochrome oxidase I and II genes. Mycologia 95:269-284.
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COX 1
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Results obtained so far with ITS, LSU and COXI validate
the separation of P. cryptogea and P. drechsleri
into two distinct and closely related phylogenetic species
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Based on preliminary results with ITS sequencing of 242 accessions from
the WOGRC there are probably 6-10 additional phylogenetic species many
originally described as either P. cryptogea or P. drechsleri
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Acknowledgements
This project has been supported by the USDA-NRI Plant Biosecurity program(2005-35605-15393 and 2008-55605-18773)