NVSL Diagnostic Catalog - Aphis - USDA - Home Landing · PDF fileDiagnostic Testing National...

Diagnostic TestingNational Veterinary Services Laboratories - Ames, Iowa

Procedure SpecimensShipping

PreservativeFY 2018 User Fee

On Test Days

Est. Time for Results*

(Days)Testing

Lab Comments

African horse sickness**ELISA $18.00Serum M - F 2 DVL/EO Two ml minimum, Testing done only for FAD

investigationsIce pack

PCR $56.00Whole blood, spleen, lung, lymph node

M - F 2 DVL/EO Testing done only for FAD investigationsIce pack

VI $52.00Whole blood, spleen, lung, lymph node

M - F 20 DVL/EO Testing done only for FAD investigationsIce pack

Aino**VN $14.00Serum M, F 4 DVL/EOIce pack

Akabane**VN $14.00Serum M, F 4 DVL/EO Two ml minimum, Contact laboratory before

shipment--(515) 337-7551.Ice pack

AnaplasmosisCELISA $18.00Serum W 4 DBL/SERO Two ml minimum, Anaplasma marginale,

cattleIce pack

CF $18.00Serum Th 4 DBL/SERO Two ml minimum, Anaplasma marginale, cattle

Ice pack

* TEST START DATE MAY BE DEPENDENT ON DAY OF WEEK AND TIME OF DAY SAMPLES ARRIVE AT TESTING LABORATORY. IF RESULTS ARE REQUIRED BY A SPECIFIC DATE PLEASE CONTACT NVSL PRIOR TO SHIPPING.

** THIS IS AN EXOTIC DISEASE. SUSPECT CASES MUST BE REPORTED TO THE STATE OR FEDERAL VETERINARIAN BEFORE SAMPLE SHIPMENT.

THE FINAL TEST REPORT WILL BE RELEASED WHEN ALL TESTING HAS BEEN COMPLETED.

of 37Print Date: 11/21/2017



On Test Days


(Days)Testing

Lab Comments

AnthraxIDENT $101.00Culture M - F 7 DBL/BI Bacillus anthracis, contact laboratory for

arrangements (515) 337-7565None

ISOLATION $101.00Blood M - F 7 DBL/BI Contact laboratory for arrangements (515) 337-7565

Clotted

ISOLATION $101.00Various M - F 7 DBL/BI Contact laboratory for arrangements (515) 337-7565

None

Aquaculture-related bacterial diseasesISOLATION $40.00Various Call 10 DBL/BI Contact laboratory for arrangements (515)

337-7565Ice Pack

Aquaculture-related viral diseasesVarious $112.00Various Call Varies DVL/BPA Contact laboratory for arrangements and

pricing (515) 337-7551Various

Aquaculture-wet lab studiesVarious $112.00Aquatic species Call Call DVL/BPA Modular system that can house a variety of

species and experimental designs in a BSL2/3 environment. Contact laboratory for more details and pricing (515) 337-7551

None

Avian adenovirus group IVI $52.00Tissue, swab M - F 18-30 DVL/AV Quail Bronchitis, chicken embryo lethal

orphanDry ice







On Test Days


(Days)Testing

Lab Comments

Avian influenza (aka fowl plague, LPAI, HPAI; subtypes H5 and H7 are reportable**)AGID $18.00Serum M - F 4 DVL/AV Two ml minimumIce pack

HI/NI $16.00Serum M - F 3 DVL/AV One ml of sera (not whole blood) recommended; sample should not exceed 1% of total body weight; contact NVSL regarding small birds. Includes HI and NI tests. Sixteen H subtypes available, user fee is per H subtype.

Ice pack

IVPI $2,632.00Virus W 12 DVL/AV Contact laboratory for arrangements (515) 337-7551

Dry ice

RRT-PCR $56.00Swab M - F 3 DVL/AV Single assay will be charged 1/2 hr rate; if a 2nd assay is performed on the same sample using the same extracted nucleic acid the 2nd assay will be charged 1/4 hr rate. Refer to sample collection guidance WI-AV-0020 online.

Dry ice

VI $52.00Tissue, swab M - F 18-30 DVL/AV Dry swabs are not acceptable, contact laboratory for further information (515) 337-7551. Refer to sample collection guidance WI-AV-0020 online.

Dry ice

Avian metapneumovirusELISA $18.00Serum M - F 3 DVL/AV One ml minimum, subtype A (UK strain),

subtype B (Hungarian strain) and subtype C (US-Colorado strain, Colorado avian pneumovirus). One ELISA charge per subtype.

Ice pack







On Test Days


(Days)Testing

Lab Comments

Avian metapneumovirusIFA $16.00Serum M - F 4 DVL/AV One ml minimumIce pack

RT-PCR $56.00Swab M - F 3-5 DVL/AVDry ice

Avian paramyxovirus serotype-1 (virulent viruses cause Newcastle disease**)HI $16.00Serum M - F 3 DVL/AV One ml of sera (not whole blood)

recommended; sample should not exceed 1% of total body weight; contact NVSL regarding small birds.

Ice pack

ICPI $896.00Virus W 12 DVL/AV Contact laboratory for arrangements (515) 337-7551

Dry ice

RRT-PCR $56.00Swab M - F 3 DVL/AV Single assay will be charged 1/2 hr rate; if a 2nd assay is performed on the same sample using the same extracted nucleic acid the 2nd assay will be charged 1/4 hr rate. Refer to sample collection guidance WI-AV-0020 online.

Dry ice

VI $52.00Hatching eggs M - F 18-30 DVL/AV Do not freezeIce pack


Dry ice







On Test Days


(Days)Testing

Lab Comments

Avian paramyxovirus serotypes 2-9HI $16.00Serum M - F 3 DVL/AV One ml of sera (not whole blood)

recommended; sample should not exceed 1% of total body weight; contact NVSL regarding small birds. Specify serotype(s) of interest.

Ice pack


Dry ice

Avian reovirusIFA $16.00Serum M - F 4 DVL/AV One ml minimum, Fahey-Crawley, infectious

synovitis, viral arthritisIce pack

VI $52.00Tissue M - F 18-30 DVL/AVDry ice

Bacterial antibiotic sensitivityAST $19.00Culture M - F 2-3 DBL/BI Antibiotic sensitivity test of bacteria except for

Mycobacterium speciesNone

Bacterial identificationIDENT $58.00Culture M - F 2-7 DBL/BINone

MALDI $8.00Culture M - F 2-7 DBL/BI MALDI Biotyper will be used for primary identification, followed by 16S rDNA sequencing (IDENT) if necessary. Please specify if you would like only MALDI Biotyper or 16s rDNA sequencing performed.

None







On Test Days


(Days)Testing

Lab Comments

Bacterial isolationISOLATION $40.00Various M - F 2-7 DBL/BIVarious

BluetongueAGID $18.00Serum M - F 2 DVL/EO Two ml minimumIce pack

CELISA $18.00Serum M,W,F 2 DVL/EO Two ml minimumIce pack

PCR $56.00Blood, spleen M - F 2 DVL/EO Four ml minimum. Refrigerate whole blood collected in EDTA. Do not freeze. A single PCR assay will be charged ½ hour rate; subsequent PCR assays (within the DVL/EO lab) on the same sample will be charged ¼ hour each.

Ice pack

VI $52.00Blood, spleen M,W,Th,F 20 DVL/EO Four ml minimum. Submit whole blood collected in EDTA or heparin. Do not freeze. Ship refrigerated overnight.

Ice pack

VI $52.00Semen M,W,Th,F 20 DVL/EO Keep frozen; test 0.5 ml non-extended semen or equivalent. Submit a minimum of 4 straws per collection.

Dry ice

VN $14.00Serum M, F 4 DVL/EO Two ml minimum, Five serotypes, user fee is per serotype

Ice pack

BotulismISOLATION $101.00Various M - F 7 DBL/BI Clostridium botulinum, contact laboratory for

arrangements (515) 337-7565Various







On Test Days


(Days)Testing

Lab Comments

BotulismTOXIN TYPING $131.00Various M - F 21 DBL/BI Clostridium botulinum, toxin typing, must be

kept cold, contact laboratory for arrangements (515) 337-7565

Various

Bovine enterovirusVI $52.00Tissue M - F 25 DVL/BPA BEVIce pack

Bovine herpesvirus 1VI $52.00Semen M - F 15 DVL/BPA Must be kept frozenDry ice, LN2

VI $52.00Tissue, blood M - F 15 DVL/BPAIce pack

VN $14.00Serum F 5 DVL/BPA Two ml minimumIce pack

Bovine herpesvirus 2VI $52.00Tissue M - F 15 DVL/BPAIce pack

VN $14.00Serum Th 5 DVL/BPA Two ml minimumIce pack

Bovine herpesvirus 4IFA $16.00Serum M - F 5-7 DVL/BPA Two ml minimum, DN599, MOVAR, bovine

cytomegalovirusIce pack

VI $52.00Tissue M - F 15 DVL/BPAIce pack







On Test Days


(Days)Testing

Lab Comments

Bovine leukosisAGID $18.00Serum M - F 2 DVL/EO Two ml minimumIce pack

ELISA $18.00Serum M,W,F 2 DVL/EO Two ml minimumIce pack

Bovine papular stomatitisIFA $16.00Serum M - F 5-7 DVL/BPA Two ml minimumIce pack


Bovine parvovirusIFA $16.00Serum M - F 3 DVL/BPA Two ml minimumIce pack


Bovine respiratory syncytial virusVI $52.00Tissue, swab M - F 15 DVL/BPA Special transport medium recommended.

Contact laboratory (515) 337-7551Ice pack

VN $14.00Serum Th 7 DVL/BPA Two ml minimumIce pack

Bovine spongiform encephalopathyELISA $0.00Fresh brain stem M - F 2 PL/PS Fresh chilled samples are the best sample for

BSE. Submit BSE samples in formalin ONLY when no fresh samples are available. Program Use Only.

Fresh chilled







On Test Days


(Days)Testing

Lab Comments

Bovine spongiform encephalopathyHISTO $0.00Brain stem M - F 10 PL/PS Brain stem essential for all submissions, only

submit formalin fixed tissue or blocks when fresh tissue is not available. Submit entire brain if possible.

Formalin fixed

IHC $0.00Brain stem M - F 10 PL/PS Brain stem essential for all submissions, only submit formalin fixed tissue or blocks when fresh tissue is not available. Submit entire brain if possible.

Formalin fixed

Bovine viral diarrheaPCR $56.00Virus isolate M - F 7-14 DVL/BPA Mucosal disease--differential for types I and

II, sample required is a viral isolateIce pack

VI $52.00Various M - F 25 DVL/BPA Tissue, swab, blood, or serum (DO NOT FREEZE WHOLE BLOOD)

Ice pack

VN $14.00Serum Th 5 DVL/BPA Two ml minimum, Types I and II, user fee is per type

Ice pack

Brucella abortus8% CARD $4.50Serum Tu,W,Th 4 DBL/SERO Two ml minimum, Bovine and porcineIce pack

BAPA $8.00Serum M 4 DBL/SERO Bang's disease, two ml minimumIce pack

BRT $40.00Milk M - F 2 DBL/MB Two ml minimumIce pack

CF COLD $18.00Serum Th 4 DBL/SERO Two ml minimumIce pack







On Test Days


(Days)Testing

Lab Comments

Brucella abortusCF WARM $18.00Serum Th 4 DBL/SERO Two ml minimumIce pack

ELISA $18.00Serum W 4 DBL/SERO Two ml minimumIce pack

FPA $28.00Serum Tu, W 4 DBL/SERO Two ml minimumIce pack

HIRT $40.00Milk M - F 2 DBL/MB Five ml minimumIce pack

PLATE, SPT $7.75Serum M 4 DBL/SERO Two ml minimumIce pack

RAP $7.25Serum Tu, Th 4 DBL/SERO Two ml minimumIce pack

RIVANOL $7.75Serum M 4 DBL/SERO Two ml minimumIce pack

TUBE $7.75Semen M - W 4 DBL/SERO Two ml minimumIce pack

TUBE, STT $7.75Serum M - W 4 DBL/SERO Two ml minimumIce pack

Brucella canisME TUBE $7.75Serum M - W 4 DBL/SERO Two ml minimumIce pack

Brucella melitensis3% CARD $4.50Serum Tu,W,Th 4 DBL/SERO Two ml minimumIce pack







On Test Days


(Days)Testing

Lab Comments

Brucella ovisCF $18.00Serum Th 4 DBL/SERO Two ml minimumIce pack

ELISA $18.00Serum Tu, Th 4 DBL/SERO Two ml minimumIce pack

BrucellosisIDENT $58.00Culture M - F 7 DBL/MBNone

ISOLATION $40.00Various M - F 10 DBL/MB Tissue, milk, semen, etc.Dry ice

PCR $58.00Culture M - F 2 DBL/MBNone

Cache ValleyVN $14.00Serum M, F 4 DVL/EO Two ml minimumIce pack

CalicivirusVI $52.00Tissue M - F 25 DVL/BPA San Miguel sea lion virusIce pack

VN $14.00Serum Th 5 DVL/BPA Nine serotypes, user fee is per serotypeIce pack

CampylobacteriosisIDENT $58.00Culture M - F 2-7 DBL/BI Contact laboratory for shipping conditions

(515) 337-7565None

ISOLATION $40.00Tissue M - F 2-7 DBL/BIIce pack







On Test Days


(Days)Testing

Lab Comments

Caprine arthritis encephalitisAGID $18.00Serum M,W,F 2 DVL/EO Two ml minimumIce pack

ELISA $18.00Serum M, W 2 DVL/EOIce pack

Chicken anemia virusPCR $56.00Tissue M - F 5 DVL/AV Additional processing fee applied if tissue

collection is needed.Dry ice

Chlamydia psittaciCF $18.00Serum M,W,F 3 DVL/EO Two ml minimum, ornithosis, parrot fever,

enzootic ovine abortionIce pack

ISO & ID $52.00Various Tu, F 14 DVL/AV Tissue, swab, or feces. Do not freeze specimens for isolation

Ice pack

PCR $56.00Various M - F 3-5 DVL/AV Tissue, swab or fecesIce pack

Chronic wasting diseaseHISTO $28.00Brain & lymph M - F 10 PL/PS Brain stem is essential for all submissions.

Whenever possible retropharyngeal lymph node should also be submitted. Formalin fixed tissue or paraffin-embedded tissue blocks.

Formalin fixed

IHC $40.00Brain & lymph M - F 10 PL/PS Formalin fixed tissue or paraffin-embedded tissue blocks. Whenever possible retropharyngeal lymph node should also be submitted along with the obex.

Formalin fixed







On Test Days


(Days)Testing

Lab Comments

Clostridium chauvoeiIDENT $58.00Culture M - F 2-7 DBL/BI BlacklegNone


Clostridium perfringensIDENT $58.00Culture M - F 2-7 DBL/BI Identification only, no toxin typingIce pack

ISOLATION $40.00Tissue M - F 2-7 DBL/BI EnterotoxemiaIce pack

Contagious ecthymaCF $18.00Serum Tu, Th 3 DVL/EO Two ml minimum, orf, contagious pustular

dermatitisIce pack

EM $56.00Lesion M - F 2 DVL/EO Lesions suggestive of FMD must be submitted to FADDL.

Ice pack

Contagious equine metritisCF $18.00Serum M & Th 4 DBL/SERO Two ml minimum, Taylorella equigenitalis.

Contact laboratory for details 515-337-7563Ice pack

ISO & ID $101.00Swab, culture M - F 7 DBL/BI Taylorella equigenitalis. Swab must be received refrigerated within 48 hrs of collection and in Amies transport medium with charcoal

Ice pack

CoronavirusIFA $16.00Serum M - F 5-7 DVL/BPA Bovine onlyIce pack







On Test Days


(Days)Testing

Lab Comments

CoronavirusVI $52.00Tissue, feces M - F 15 DVL/BPA Bovine or porcineIce pack

VN $14.00Serum M - F 4 DVL/BPA Two ml minimum, TGE onlyIce pack

CorynebacteriumIDENT $58.00Culture M - F 2-7 DBL/BINone


Coryza, infectiousIDENT $58.00Culture M - F 2-7 DBL/BINone

ISOLATION $40.00Tissue, swab M - F 2-7 DBL/BI Haemophilus paragallinarumIce pack

Coryza, turkeyIDENT $58.00Culture M - F 2-7 DBL/BI Bordetella aviumNone

ISOLATION $40.00Tissue, swab M - F 2-7 DBL/BIIce pack

CysticercosisIDENT $0.00Tissue M - F 2 PL/PCPFormalin

DourineCF $18.00Serum M - F 4 DBL/SERO Two ml minimum, Trypanosoma equiperdumIce pack







On Test Days


(Days)Testing

Lab Comments

Duck viral enteritisIFA $16.00Serum M - F 4 DVL/AV Two ml minimumIce pack

PCR $56.00Tissue M - F 3-5 DVL/AV Liver preferred, or contact laboratory (515) 337-7551

Ice pack

Electron microscopy for morphologic virus identificationEM $56.00Various M - F 2-5 DVL/ALL Virus isolates, intestinal contents, lesions.

Contact laboratory for detials (515)337-7551Dry ice

EncephalomyocarditisVI $52.00Tissue M - F 15 DVL/BPA Heart, lung, liver, or kidneyIce pack

VN $14.00Various Tu 3 DVL/BPA Two ml minimum--serumIce pack

Epizootic hemorrhagic diseaseAGID $18.00Serum M - F 2 DVL/EO Two ml minimumIce pack

PCR $56.00Blood, spleen M - F 2 DVL/EO Four ml minimum. Refrigerate whole blood collected in EDTA. Do not freeze. A single PCR assay will be charged ½ hour rate; subsequent PCR assays (within the DVL/EO lab) on the same sample will be charged ¼ hour each.

Ice pack

VI $52.00Blood, spleen W,Th,F 20 DVL/EO Four ml minimum. Submit whole blood collected in EDTA or heparin. Do not freeze. Ship refrigerated overnight.

Ice pack







On Test Days


(Days)Testing

Lab Comments

Epizootic hemorrhagic diseaseVI $52.00Semen W,Th,F 20 DVL/EO Keep frozen; test 0.5 ml non-extended semen

or equivalent. Submit a minimum of 4 straws per collection.

Dry ice

VN $14.00Serum M, F 4 DVL/EO Two ml minimum, type 1, 2; user fee is per serotype

Ice pack

Equine adenovirusVI $52.00Nasal swabs,

tracheal washW, Th 16 DVL/EODry ice

VN $14.00Serum M, F 4 DVL/EO Two ml minimum, EquineIce pack

Equine encephalomyelitisCF $18.00Serum W 3 DVL/EO Two ml minimum, user fee is per strainIce pack

HI $16.00Serum W 3 DVL/EO Two ml minimum, user fee is per strainIce pack

IgM ELISA $18.00Serum M, Th 2 DVL/EO Two ml minimum, user fee is per strainIce pack

PCR $56.00Brain M - F 2 DVL/EO May ship refrigerated overnight with ice pack. A single PCR assay (EEE or WEE) will be charged ½ hour rate; subsequent PCR assays (within the DVL/EO lab) on the same sample will be charged ¼ hour each.

Dry ice







On Test Days


(Days)Testing

Lab Comments

Equine encephalomyelitisPRNT $19.00Serum Tu 4 DVL/EO Two ml minimum, user fee is per strain.

Equine serum: EEE or WEE PRNT may not be requested individually; equine samples are tested for antibodies for both EEE and WEE.

Ice pack

VI $52.00Brain, CSF, whole blood

W,Th,F 14 DVL/EO Must perform rabies test on mammalian brain tissue prior to submission. Fresh tissue may be sent on wet ice overnight. Whole blood should be shipped refrigerated. Sample of choice is fresh brain tissue.

Dry ice

Equine herpesvirus 1PCR $56.00Tissue, nasal

swab, whole blood

Varies 2 DVL/EO Strain Differentiation. Ship whole blood refrigerated. Contact laboratory to arrange (515) 337-7551

Dry ice

PCR $56.00Tissue, nasal swab, whole blood

Varies 2 DVL/EO Includes EHV-4 PCR at no additional charge. Ship whole blood refrigerated. This PCR assay will be charged ½ hour rate; subsequent PCR assays (within the DVL/EO lab) on the same sample will be charged ¼ hour each.

Dry ice

VI $52.00Tissue, nasal swab, whole blood

W, Th 14 DVL/EO Ship whole blood refrigeratedDry ice

VN $14.00Serum M, F 4 DVL/EO Two ml minimum, does not differentiate between herpesvirus 1 and 4

Ice pack







On Test Days


(Days)Testing

Lab Comments

Equine herpesvirus 2VI $52.00Swabs, whole

bloodW, Th 14 DVL/EO Cytomegalovirus, Ship whole blood

refrigeratedDry ice

VN $14.00Serum M, F 7 DVL/EO Two ml minimumIce pack

Equine herpesvirus 3VI $52.00Lesions, swabs W, Th 14 DVL/EO Coital exanthemaDry ice


Equine herpesvirus 4PCR $56.00Tissue, nasal

swab, whole blood

Varies 2 DVL/EO Includes EHV-1 PCR at no additional charge. Ship whole blood refrigerated. This PCR assay will be charged ½ hour rate; subsequent PCR assays (within the DVL/EO lab) on the same sample will be charged ¼ hour each.

Dry ice

VI $52.00Tissue, swab, whole blood

W, Th 14 DVL/EO Ship whole blood refrigeratedDry ice

Equine infectious anemiaAGID $18.00Serum M - F 2 DVL/EO Two ml minimum, no charge for confirmation

of positivesIce pack

ELISA $18.00Serum M - F 2 DVL/EO Two ml minimumIce pack

WBLOT $112.00Serum M - F 3 DVL/EO Contact laboratory for details (515) 337-7551Ice pack







On Test Days


(Days)Testing

Lab Comments

Equine influenzaHI $16.00Serum W 2 DVL/EO User fee is per antigen; three antigens are

used for each HIIce pack

PCR $56.00Tissue, nasal swab

Varies 2 DVL/EODry ice

VI $52.00Tissue, nasal swab

M,W-F 20 DVL/EO A1 - H7N7, A2 - H3N8 (Extra time required for isolate identification)

Dry ice

Equine rhinitis virusVI $52.00Lung tissue,

nasal swabW, Th 16 DVL/EO Extra time required for isolate identificationDry ice

VN $14.00Serum M, F 4 DVL/EO Rhino 1 and 2 serotypes; user fee is per serotype

Ice Pack

Equine viral arteritisVI $52.00Semen M - F 15-20 DVL/EO Five ml minimumDry ice

VI $52.00Tissue, swab, whole blood

M - F 14 DVL/EO Ship whole blood refrigeratedDry ice


ErysipelasIDENT $58.00Culture M - F 2-7 DBL/BI Erysipelothrix rhusiopathiaeNone







On Test Days


(Days)Testing

Lab Comments

ErysipelasISOLATION $40.00Tissue M - F 2-7 DBL/BIIce pack

Fetal bovine serum safety testSI $1,160.00Serum Th 35 DVL/EO Contact State or Federal veterinarian for

special requirements for testing before submitting serum

Dry ice

Fowl choleraDNA FP $64.00Culture M - F 30-60 DBL/BINone

GDPT $20.00Culture M - F 7 DBL/BI Somatic antigen typingNone

IDENT $58.00Culture M - F 2-7 DBL/BINone

ISOLATION $40.00Tissue M - F 2-7 DBL/BI Pasteurella multocidaIce pack

Fowl poxIFA $16.00Serum M - F 4 DVL/AV One ml minimum, Avian poxIce pack

VI $52.00Tissue M - F 30 DVL/AVDry ice

GlandersCF $18.00Serum M - F 4 DBL/SERO Two ml minimum, Burkholderia malleiIce pack







On Test Days


(Days)Testing

Lab Comments

GlandersIDENT $101.00Culture M - F 21 DBL/BI (Burkholderia mallei) Contact laboratory for

contact laboratory for arrangements (515) 337-7565

None

ISOLATION $101.00Various M - F 7 DBL/BI (Burkholderia mallei) Contact laboratory for contact laboratory for arrangements (515) 337-7565

Ice pack

Heartwater**PCR $56.00Blood (EDTA) M - F 4 DVL/BPA Testing done only for FAD investigations and

importation of animals from other countries. Importation testing can take up to 14 days.

Ice pack

PCR $56.00Tissue M - F 4 DVL/BPAIce pack

HistopathologyHISTO $28.00Fixed tissue M - F 5 PL/PS Cost per slideFormalin

fixed

Ibaraki**VN $14.00Serum M, F 4 DVL/EO Testing done only for FAD investigations and

importation of animals from other countriesIce pack

Import testing for avian-origin materialCI $4,180.00Varies M - F 35 DVL/AV 20 birds inoculated with material and tested

for antibody to avian influenza, Newcastle disease, and adenovirus 127 at 21 dpi

Dry ice







On Test Days


(Days)Testing

Lab Comments

Infectious bursal diseaseAGID $18.00Serum M - F 4 DVL/AV One ml minimum, Gumboro diseaseIce pack


Infectious laryngotracheitis (aka Gallid herpesvirus 1)IFA $16.00Serum M - F 4 DVL/AV One ml minimumIce pack

PCR $56.00Tissue, swab M - F 3-5 DVL/AVIce pack


Johne's diseaseAGID $18.00Serum Varies 4 DBL/SERO Two ml minimumIce pack

CF $18.00Serum Tu 4 DBL/SERO Two ml minimum, Mycobacterium paratuberculosis

Ice pack

ELISA $18.00Serum, milk Th 4 DBL/SERO Two ml minimumIce pack

FECAL PCR $33.00Feces M - F 14 DBL/MB Two gram minimumIce pack

IS1311 $101.00Culture, tissue M - F 5 DBL/MB IS1311 Sheep and cattle paratuberculosis strain differentiation

None

ISO & ID $79.00Tissue, feces, culture

M - W 56 DBL/MB Bactec 460, Trek ESP, MGIT 960, HEYIce pack







On Test Days


(Days)Testing

Lab Comments

KlebsiellaIDENT $58.00Culture M - F 2-7 DBL/BI Serotyping is not availableNone


LeptospirosisISOLATION $56.00Kidney, liver,

urine fresh not frozen

M - F 30-90 DBL/BI Contact laboratory before submitting (515) 337-7595

Ice pack

MAT $26.00Serum W 2-7 DBL/BI Samples must be received by 12:00 pm on Wednesday for testing; additional charge for more than 5 serovars

Ice pack

ListeriosisIDENT $58.00Culture M - F 2-7 DBL/BI Listeria spp.None


Malignant catarrhal feverIPT $17.00Serum W 5-7 DVL/BPA Two ml minimum, The IPT will be conducted

if the paperwork submitted doesn't specify a test. All antibody positive IPT samples will be tested by the VN, resulting in two test charges (IPT and VN)

Ice pack

PCR $56.00Blood M - F 7-14 DVL/BPA Differentiates between alcelaphine herpesvirus 1 and ovine herpesvirus 2

EDTA







On Test Days


(Days)Testing

Lab Comments

Malignant catarrhal feverPCR $56.00Tissue M - F 7-14 DVL/BPA Differentiates between alcelaphine

herpesvirus 1 and ovine herpesvirus 2Ice pack

VN $14.00Serum Th 7 DVL/BPA Two ml minimum, The IPT will be conducted if the paperwork submitted doesn't specify the VN test. All antibody positive IPT samples will be tested by the VN, resulting in two test charges (IPT and VN)

Ice pack

Marek's diseaseAGID $18.00Serum M - F 4 DVL/AV One ml minimumIce pack

MelioidosisIDENT $101.00Culture M - F 21 DBL/BI (Burkholderia pseudomallei) Contact

laboratory for arrangements (515) 337-7565None

ISOLATION $101.00Various M - F 7 DBL/BI (Burkholderia pseudomallei) Contact laboratory for arrangements (515) 337-7565

Ice pack

MycobacteriumAFS $28.00Fluid, culture M - F 2 DBL/MB Direct acid fast stain on clinical specimensVarious

AST $114.00Culture Call 28 DBL/MB Mycobacterium tuberculosis complex, antibiotic sensitivity test performed on M. tuberculosis complex only, contact laboratory at (515)337-7388 for test schedule

None

DNA PROBE $89.00Culture M - F 2 DBL/MB DNA probeVarious







On Test Days


(Days)Testing

Lab Comments

MycobacteriumDPP-Cervid $12.00Serum M - F 5 DBL/SERO DPP test for white-tail deer, elk, red deer,

reindeer, and fallow deer only. Please contact testing lab at 515-337-7563 for test availability before submitting samples.

Ice pack

DPP-Elephant $12.00Serum M - F 5 DBL/SERO DPP test for elephant. Please contact testing lab at 515-337-7563 for test availability before submitting samples.

Ice pack

GAMMA INF $112.00Heparinized blood DBL/SERO Please direct all inquiries for testing to the Cattle Health Center, at 301-851-3502 and/or [email protected].

None

HISTO $28.00Block containing lesion

M - F 1 PL/PS Formalin fixed tissue - paraffin-embedded tissue blocks. Cost is per slide.

Formalin fixed

IDENT $58.00Culture M - F 56 DBL/MB Identification of acid fast bacteria includes biochemical analysis and 16s rDNA sequencing analysis

None

ISOLATION $40.00Tissue, swabs, washes

M - F 60 DBL/MB Contact laboratory at (515) 337-7388 for details. Shipping preservative - ice pack or Na Borate

Various

PCR $112.00Fixed tissue containing lesion

M - F 10 PL/PS Formalin fixed tissues or paraffin blocks should not be decalcified. Tissue should be in formalin less than 10 days. Acid fast bacteria must be present to perform PCR testing.

Formalin fixed







On Test Days


(Days)Testing

Lab Comments

MycobacteriumTRUNKW $40.00Fluid M - F 60 DBL/MB Elephant trunk washings - See Guide for the

control of tuberculosis in elephants. 2003. Available at www.elephantcare.org

Ice pack

MycoplasmaHI $16.00Serum M - F 5 DBL/BI Two ml minimum, Mycoplasma gallisepticum,

M. meleagridis, and M. synoviaeIce pack

PLATE $7.75Serum M - F 3 DBL/BI Two ml minimum, Mycoplasma gallisepticum, M. meleagridis, and M. synoviae

Ice pack

Ornithobacterium rhinotrachealeIDENT $58.00Culture M - F 2-7 DBL/BI ORTNone

ISOLATION $40.00Various M - F 3-14 DBL/BI ORTVarious

Ovine progressive pneumoniaAGID $18.00Serum M - F 2 DVL/EO Two ml minimum, Maedi-VisnaIce pack

Parainfluenza-3VI $52.00Tissue, swab M - F 15 DVL/BPA Nasal swab. Shipping fever, virus pneumoniaIce pack

VN $14.00Serum Th 7 DVL/BPAIce pack







On Test Days


(Days)Testing

Lab Comments

Parasites, bloodMX $31.00Whole blood M - F 3 PL/PPE Examination of Giemsa-stained blood films

for Anaplasma, Babesia, Theileria, trypanosomes. Send whole blood in EDTA on ice packs, chilled, but not frozen.

EDTA

Parasites, externalIDENT $0.00Parasite M - F 3 PL/PPE Preserve in ethanol or isopropyl alcohol. ID

to species: ticks, fly larvae, mange mites, lice, fleas.

Alcohol

ParatyphoidTUBE $7.75Serum M - Th 4 DBL/SERO Two ml minimum, Salmonella abortus-equi,

equidaeIce pack

Piroplasmosis, bovineCF $18.00Serum W 4 DBL/SERO Two ml minimum, Babesia bovis and B.

bigemina, user fee is per speciesIce pack

Piroplasmosis, equineCELISA $18.00Serum M - F 4 DBL/SERO Two ml minimum, Babesia caballi and

Babesia (Theileria) equi, user fee is per species.

Ice pack

CF $18.00Serum M - F 4 DBL/SERO Two ml minimum, Babesia caballi and Babesia (Theileria) equi, user fee is per species

Ice pack

IFA $16.00Serum Tu,W,Th 4 DBL/SERO Two ml minimum, Babesia caballi and Babesia (Theileria) equi, user fee is per species.

Ice pack







On Test Days


(Days)Testing

Lab Comments

Porcine delta coronavirusPCR $56.00Feces, tissue,

rectal swabs4 DVL/BPA This PCR assay detects and differentiates

porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine deltacorona virus. Contact Laboratory for arrangements (515) 337-7551.

Ice pack

VI $52.00Feces, tissue, rectal swabs

M - F 35 DVL/BPAIce pack

Porcine epidemic diarrheaPCR $56.00Feces, tissue,

rectal swabsCall 4 DVL/BPA This PCR assay detects and differentiates

porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine deltacorona virus. Contact laboratory for arrangements (515) 337-7551.

Ice pack



Porcine hemagglutinating encephalomyelitis virusHI $16.00Serum M - F 2 DVL/BPA Two ml minimumIce pack

VI $52.00Tissue M - F 15 DVL/BPA Lung or brainIce pack

Porcine parvovirusHI $16.00Various Th 2 DVL/BPA Serum or fetal fluid--two ml minimumIce pack








On Test Days


(Days)Testing

Lab Comments

Porcine reproductive and respiratory syndromeELISA $18.00Serum M - F 3 DVL/BPA PRRSIce pack

IFA $16.00Serum M - F 3 DVL/BPA US vs. European differentiation, two user fee charges per serum. 3 day time frame for results is not applicable to export testing-time line negotiated. Contact laboratory (515) 337-7551.

Ice pack

VI $52.00Tissue, serum M - Th 16 DVL/BPA Two user fee charges if isolation on MARC-145 & SAM cell cultures. 16 day time frame for results is not applicable to export testing-time line negotiated. Test set up can not occur on Fridays or the day before a holiday. Contact laboratory (515) 337-7551

Ice pack

Porcine rubulavirus**HI $16.00Serum M - F 2 DVL/BPA a/k/a Blue eye paramyxovirus, Two ml

minimum, porcine BEPIce pack

VI $52.00Tissue, swab M - F 21 DVL/BPA a/k/a Blue eye paramyxovirusIce pack

Porcine sapelovirusIFA $16.00Serum M - F 5-7 DVL/BPA Formerly Porcine enterovirus A or PEV 8Ice pack

VI $52.00Tissue M - F 25 DVL/BPA Formerly Porcine enterovirus A or PEV 8Ice pack

Porcine teschovirusIFA $16.00Serum M - F 5-7 DVL/BPA 7 groups, user fee is per group, (formerly

PEV 1-7)Ice pack







On Test Days


(Days)Testing

Lab Comments

Porcine teschovirusVI $52.00Tissue M - F 25 DVL/BPA SMEDI (Stillbirth, mummification, embryonic

death & infertility), formerly PEV 1-7 & porcine polioencephaloyelitis, if suspect Teshan disease (PTV1) contact state of federal vet before shipment

Ice pack

Potomac horse feverIFA $16.00Serum M - F 3 DVL/EO Two ml minimum, Ehrlichia risticiiIce pack

PoxEM $56.00Tissue M - F 5 DVL/BPA ID virus in lesionIce pack


PseudocowpoxIFA $16.00Serum M - F 3 DVL/BPA Two ml minimumIce pack


PseudomonasIDENT $58.00Culture M - F 7 DBL/BINone

ISOLATION $40.00Tissue M - F 7 DBL/BIIce pack







On Test Days


(Days)Testing

Lab Comments

PseudorabiesELISA $18.00Serum M - F 2 DVL/BPA Two ml minimum, Screening or differential for

gB. Aujeszky's disease, infectious bulbar paralysis, mad itch

Ice pack

LAT $18.00Serum M - F 2 DVL/BPA Two ml minimumIce pack


VN $14.00Serum F 3 DVL/BPA Two ml minimumIce pack

Psittacine herpesvirusIFA $16.00Serum M - F 4 DVL/AV One ml minimumIce pack

Pullorum-typhoidAGGL $7.75Serum M - Th 3 DBL/BIIce pack

ISOLATION $40.00Tissue M - F 7 DBL/BI Pullorum disease will be reported to the State Veterinarian and NPIP Staff Veterinarian

Ice pack

Q-feverCF $18.00Serum Tu, Th 3 DVL/EO Two ml minimum, Coxiella burnettiIce pack

ELISA $18.00Serum Varies 3 DVL/EO Two ml minimumIce pack

ReovirusIFA $16.00Serum M - F 5-7 DVL/BPAIce pack







On Test Days


(Days)Testing

Lab Comments

ReovirusVI $52.00Tissue M - F 15 DVL/BPA Porcine onlyIce pack

RotavirusEM $56.00Feces M - F 5 DVL/BPA IdentificationIce pack

IFA $16.00Serum M - F 3 DVL/BPA Bovine and porcineIce pack

VI $52.00Tissue, feces M - F 21 DVL/BPAIce pack

Salmonella (all subspecies)ISOLATION $40.00Various M - F 7 DBL/BI Includes S. pullorumVarious

PFGE $64.00Culture Tu - Th 7 DBL/BINone

PHAGE TY $46.00Culture M - F 5 DBL/BI Salmonella typhimurium phage typingNone

PHAGE TY $26.00Culture M - F 5 DBL/BI Salmonella enteritidis phage typingNone

SEROTYP $40.00Culture M - F 14 DBL/BINone

Salmonella enteritidisSEROTYP $28.00Culture M - F 2-3 DBL/BI Accelerated Salmonella Enteritidis Rule OutNone

Salmonella typhimuriumAGGL $7.75Serum M - Th 3 DBL/BIIce pack







On Test Days


(Days)Testing

Lab Comments

Schmallenberg**VN $14.00Serum 4 M, F DVL/EO Two ml minimumIce pack

ScrapieHISTO $0.00Brain stem M - F 5 PL/PS Formalin fixed tissue or paraffin-embedded

blocks. Submit entire brain if possible.Formalin

fixed

IHC $0.00Brain & lymph M - F 10 PL/PS Formalin fixed tissue or paraffin-embedded blocks. Brain stem is essential for all submissions. Whenever possible retropharyngeal lymph node and tonsil should also be submitted.

Formalin fixed

PrP-Codon $33.00Whole blood, tissue, FTA card

Varies 10 DBL/MB Srapie susceptibility genotyping for codons 171, 136, 141, 154. All codons are tested automatically. See approved laboratories list if single codon testing is desired. Contact 515.337.7388 for more information.

None

Sequencing identification/characterizationSEQUENCING VariesVaries Varies Varies ALL Whole and partial genome sequencing and

analysis are available. Pricing varies based on sample and analysis. Contact the appropriate lab below for sample requirements, pricing and scheduling. Bacteriology (515) 337-7388, Virology (515) 337-7551

Varies

Serum screenCHEM $0.00Serum, blood M - F Varies PL/PCP Contact laboratory for details (515) 337-7065Ice pack







On Test Days


(Days)Testing

Lab Comments

StreptococcusIDENT $58.00Culture M - F 2-7 DBL/BI Including stranglesNone

ISOLATION $40.00Tissue, swab M - F 2-7 DBL/BI Including stranglesIce pack

Swine influenzaHI $16.00Serum M - W 3 DVL/BPA Multiple serotypes, user fee is per serotypeIce pack

HI/NI $16.00Viral isolates M - F 5 DVL/BPA For subtyping SIV isolates. User fee will be two HI charges per sample. If isolate is of insufficient viral titer, virus isolation charges may apply instead of HI charges.

Dry ice


Transmissible gastroenteritisFATST $31.00Tissue M - F 5 DVL/BPAIce pack

PCR $56.00Feces, tissue, rectal swabs

Call 4 DVL/BPA This PCR assay detects and differentiates porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine deltacorona virus. Contact Laboratory for arrangements (515) 337-7551.

Ice pack



VN $14.00Serum M - F 5 DVL/BPA Two ml minimumIce pack







On Test Days


(Days)Testing

Lab Comments

Tuberculosis (See also Mycobacterium)DPP-Cervid $12.00Serum M - F 5 DBL/SERO DPP test for white-tail deer, elk, red deer,

reindeer, and fallow deer only. Please contact testing lab at 515-337-7563 for test availability before submitting samples.

Ice pack

DPP-Elephant $12.00Serum M - F 5 DBL/SERO DPP test for elephant. Please contact testing lab at 515-337-7563 for test availability before submitting samples.

Ice pack

GAMMA INF $112.00Heparinized blood DBL/SERO Please direct all inquiries for testing to the Cattle Health Center, at 301-851-3502 and/or [email protected].

None

TularemiaIDENT $101.00Culture M - F 7 DBL/BI Contact laboratory for arrangements (515)

337-7565None

ISOLATION $101.00Tissue M - F 7 DBL/BI Contact laboratory for arrangements (515) 337-7565

Ice pack

PLATE $7.75Serum M - F 4 DBL/SERO Francisella tularensis, Two ml minimumIce pack

Vesicular stomatitisCELISA $18.00Serum M - F 2 DVL/BPA Two ml minimum, New Jersey and Indiana

types, two user fee charges per serumIce pack

CF $18.00Serum M - F 2 DVL/BPA Two ml minimum, New Jersey and Indiana types, two user fee charges per serum

Ice pack







On Test Days


(Days)Testing

Lab Comments

Vesicular stomatitisPCR $56.00Tissue, vesicular

fluid, lesion swabM - F 7 DVL/BPA RUMINANT AND PORCINE SAMPLES--

SEND TO FADDL; EQUINE SAMPLES--SEND TO DVL. Notify State veterinarian/Federal veterinarian.

Ice pack

VI $52.00Tissue, vesicular fluid, lesion swab

M - F 7 DVL/BPA RUMINANT AND PORCINE SAMPLES--SEND TO FADDL; EQUINE SAMPLES--SEND TO DVL. Notify State veterinarian/Federal veterinarian.

Ice pack

VN $14.00Serum M,W,F 3 DVL/BPA Two ml minimum, New Jersey and Indiana types, two user fee charges per serum

Ice pack

VibriosisIDENT $58.00Culture M - F 2-7 DBL/BINone

ISOLATION $40.00Culture M - F 7 DBL/BI Vibrio spp.None

Viral abortionVI $52.00Various M - F 14-28 DVL/BPA Serum or fetal fluid--two ml minimum, and

tissue. Bovine and porcineIce pack

West nile virusIgM ELISA $18.00Serum M, Th 2 DVL/EO Two ml minimumIce pack







On Test Days


(Days)Testing

Lab Comments

West nile virusPCR $56.00Brain M - F 2 DVL/EO May ship sample refrigerated overnight with

ice pack. A single PCR assay will be charged ½ hour rate; subsequent PCR assays (within the DVL/EO lab) on the same sample will be charged ¼ hour each.

Dry ice

PRNT $19.00Serum F 5 DVL/EO Two ml minimumIce pack

VI $52.00Brain W,Th,F 14 DVL/EO (See equine encephalomyelitis) PCR preferred for WNV detection

Dry ice

VN $14.00Serum F 4 DVL/EO Two ml minimum, Contact laboratory to arrange. (515) 337-7551

Ice pack

Yersinia spp.IDENT $58.00Culture M - F 2-7 DBL/BI Yersiniosis, not plague. Contact laboratory

for plague (515) 337-7565None

ISOLATION $40.00Various M - F 2-7 DBL/BIIce pack





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