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Transcript of Nutriplant sd ag 2012
1
According to Bray1 et al.,
crops produce only 24%
of their yield potential
1 Mean of 6 crops: barley, maize, potatoes, soybeans, sugar beets, wheat
Stress Reduces Yields
Bray, Bailey-Serres and Weretilnyk. 2000. Responses to abiotic stresses. In: W Gruissem, B.
Biology of Plants. American Society of Plant Physiologists, Rockville, MD, pp 1158–1249.
Stress Reduces Yields
2
Stress Reduces Yields
By reducing the effects of
abiotic and biotic stress,
Nutriplant products
increase both yield and
quality of crops
3
• Nutriplant SD/SL seed treatment
• Nutriplant AG foliar nutrition
4
Product Description Nutritional supplement for emergence and root growth
• Dry Nutriplant SD for application in
the field at planting
• Liquid Nutriplant SL for commercial
and in-furrow application
Available in 2 forms:
5
Nutriplant SD/SL increases crop yield by:
• Providing essential nutrients for
development of strong shoots and roots
• Enhancing seedling vigor
• Stimulating root hair growth
• Reducing effects of abiotic stress
• Reducing effects of biotic stress
• Overcoming negative effects of pesticides
applied to seeds
Modes of Action
6
Nutriplant SD/SL provides nutrients
essential for development of strong
shoots and roots
Mode of Action
7
In roots Nutriplant SD increased the
content of copper (Cu) by 35%, iron (Fe)
by 53%, manganese (Mn) by 9.2% and
zinc (Zn) by 16% over untreated control
Essential Nutrients
Roots Shoots
La
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es
ult
s
In shoots Nutriplant SD increased the
content of copper (Cu) by 32%, iron (Fe)
by 46%, manganese (Mn) by 71% and zinc
(Zn) by 54% over untreated control
Source: Cytozyme Laboratories Inc., USA
0.217
1.02
0.168
1.36
0.287
1.49
0.287
2.10
0
0.5
1
1.5
2
2.5
Cu Fe Mn Zn
Nu
trie
nt
co
nte
nt
(mic
rog
ram
s/s
ee
dli
ng
)
0.199
1.04
0.130
0.878
0.267
1.59
0.142
1.02
0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
1.8
Cu Fe Mn Zn
Nu
trie
nt
co
nte
nt
(mic
rog
ram
s/s
ee
dli
ng
)
8
Nutriplant SD/SL increases
seedling vigor
Mode of Action
9
Nutriplant SD increased both root and shoot
mass of corn seedlings over control
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Shoot mass (mg/seedling) Root mass (mg/seedling)
Source: Cytozyme Laboratories Inc., USA
10
Increased Seedling Vigor
Nutriplant SD increased fresh shoot mass by an average of
75% and fresh root mass by an average of 47% in Pioneer,
Dekalb and other leading corn hybrid
Root mass Shoot mass
Source: Cytozyme Laboratories Inc., USA La
bo
rato
ry R
esu
lts
135
107
169
239
160
193
0
50
100
150
200
250
300
PioneerHybrid
Hybrid X DekalbHybrid
Fre
sh
ro
ot
ma
ss
(m
g/s
ee
dli
ng
)
Control Nutriplant SD
135
88.7
132
240
149
236
0
50
100
150
200
250
300
PioneerHybrid
Hybrid X DekalbHybrid
Fre
sh
sh
oo
t m
as
s
(mg
/se
ed
lin
g)
Control Nutriplant SD
11
Nutriplant SD/SL stimulates
root hair growth
Mode of Action
12
Nutriplant SD produced more root hairs for
better water and nutrient uptake
La
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rato
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esu
lts
Source: Cytozyme Laboratories, Inc., USA
13
Nutriplant SD/SL reduces
impact of abiotic stress
Mode of Action
14
Irrigated Winter Wheat
Grain Yields
Year Control
(kg/ha)
Nutriplant SD
(kg/ha)
Increase
(kg/ha)
Increase
(%)
2002 4685 4955 270 5.8
2003 6303 6768 465 7.4
2004 4679 4955 276 5.9
2006* 2157 2508 351 16.3
*Low yields are a result of extreme heat early in the season
Nutriplant SD increased irrigated
winter wheat yield by 5.8% to 16.3%
over control
The largest increase occurred when
plants were under abiotic stress
conditions early in the season
Application rate: 1.5 ml/kg of seed
Source: IRF, USA, 2002, 2003, 2004, 2006
Fie
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16.3
15
Grain Yields
Year Control
(kg/ha)
Nutriplant SD
(kg/ha)
Increase
(kg/ha)
Increase
(%)
2004 1685 1915 230 13.6
2005 1058 1753 695 65.7
2006* 715 1160 445 62.2
*Low yields are a result of extreme heat early in the season
Dry Land Winter Wheat
Nutriplant SD increased dry land
winter wheat yield by 13.6% to
65.7% over control
The largest increase occurred when
plants were under abiotic stress
conditions early in the season
Application rate: 1.5 ml/kg of seed
Source: IRF, USA, 2004, 2005, 2006 Fie
ld R
esu
lts
65.7
62.2
16
Nutriplant SD/SL reduces
impact of biotic stress
Mode of Action
17
Effects of Nutriplant SD/SL on Eyespot reduction
in winter wheat seedlings treated with Baytan
Treatment
Total
Eyespot
(%)
Severe
Eyespot (%)
Reduction (%)
Total
Eyespot
Severe
Eyespot
Baytan™ 32.0 12.3 - -
Baytan and
Nutriplant SD/SL
23.0 7.0 28 43
Nutriplant SD/SL reduced total
Eyespot disease symptoms by 28%
and severe symptoms by 43% over
seeds treated with Baytan fungicide
alone
Source: Scotland College, Dunes, UK
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43 28
18
Effects of Nutriplant SD/SL on
Fusarium Infection of Corn
Treatment Fusarium Infection (%)
Control 16.4
Nutriplant SD/SL 11.1
Nutriplant SD/SL reduced the
incidence of Fusarium to 11.1%
compared to 16.4% in the control, a
reduction of 32%
Source: Agricultural Research Institute, West Germany Fie
ld R
esu
lts
19
Nutriplant SD/SL overcomes
negative effects of
pesticides applied to seeds
Mode of Action
20
139
319
0
50
100
150
200
250
300
350
Control Nutriplant SD
Fre
sh
ro
ot
ma
ss
(m
g/s
ee
dli
ng
)
Nutriplant SD increased root mass by 130% and shoot mass by
88% in seeds treated with Poncho 250 insecticide
Nutriplant SD Overcomes Negative
Effects of Poncho™ Insecticide
Poncho™ 250 contains 40% of the active ingredient clothianidin applied at 0.25mg/kernel
Source: Cytozyme Laboratories Inc., USA La
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esu
lts
206
388
0
50
100
150
200
250
300
350
400
Control Nutriplant SD
Fre
sh
sh
oo
t m
as
s (
mg
/se
ed
lin
g)
21
Nutriplant SD Overcomes Negative
Effects of Poncho™ Insecticide
Treatment with Nutriplant SD
restored the seedling vigor of
seeds treated with Poncho 250
insecticide
Control Treated
La
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Poncho™ 250 contains 40% of the active ingredient clothianidin applied at 0.25mg/kernel
Source: Cytozyme Laboratories Inc., USA
22
Summary By providing nutrients, increased seedling vigor,
stimulation of root hair development, reduction of
negative effects of abiotic and biotic stress, and pesticide
application, Nutriplant SD/SL increases crop yield
23
A foliar nutritional supplement to enhance crop growth
Product Description
Available in 2 sizes:
• 2.5 gallon jug
• 30 gallon drum
24
Stress Reduces Yields
• Increases photosynthesis
• Improves uptake of nutrients through the root
system
• High antioxidant activity to reduce the effects of
free radicals
• Up-regulates genes involved in plant response to
abiotic and biotic stress
• Reduces negative effects of abiotic stress of heat,
freezing and drought
• Reduces negative effects of plant diseases
Modes of Action
25
Nutriplant AG
increases
photosynthesis
26
Source: Cotton and rice data from the University of Arkansas, USA
Tea data from the UPASI Tea Research Foundation, India
Increases Photosynthesis
Higher photosynthesis results in higher yields
Fie
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Nutriplant AG increased photosynthesis
by an average of 16%
27
Nutriplant AG
significantly increased
the uptake of nutrients
through the roots
28
Model plant: Corn Source: Cytozyme Laboratories, Inc., USA
Nutriplant AG Increases Nutrient Uptake Through Roots
Better nutrient uptake improves plant growth and yield
La
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rato
ry R
esu
lts
Plants treated with Nutriplant AG
absorbed 230% more copper (Cu),
69% more iron (Fe), 63% more
manganese (Mn) and 130% more
zinc (Zn)
29
Nutriplant AG has high levels of antioxidants
Healthy fruit Oxidized fruit
30
High antioxidant activity in Nutriplant AG protects
plants from damage caused by free radicals
Nutriplant AG has High Antioxidant Activity
La
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Nutriplant AG has 6.5 times
higher antioxidant activity
than competitor products
Source: Cytozyme Laboratories Inc., USA
453
2,960
0
1,000
2,000
3,000
Competitor Nutriplant AG
6.5 times more
31
Nutriplant AG
up-regulates genes
involved in plant
response to stress
32
Effects of Nutriplant AG on gene
expression was studied using
Arabidopsis thaliana model plants
Gene Expression Test
33
(Image Source: http://www.mun.ca/biology/scarr/cDNA_microarray_Assay_of_Gene_Expression.html)
34
Nutriplant AG
Up-regulates
Genes
Nutriplant AG increased representation of
up-regulated genes in 2 categories:
1) Response to abiotic stimulus
2) Response to stress
La
bo
rato
ry R
esu
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Model plant: Arabidopsis thaliana
Source: Cytozyme Labs Inc. in cooperation with Arizona State University and Innovabio of Salt Lake Community College, USA
0
5
10
15
20
25
Per
cen
tage
of
the
gen
om
e re
pre
sen
ted
Tested whole genome (29,500 genes) Found 37 up-regulated genes
Microarray Test
35
Through up-regulation of
specific genes,
Nutriplant AG reduces
damage caused by
abiotic and biotic stress
36
Nutriplant AG Reduces Damage Caused by Drought Stress
Model plant: Tomato
Treatment: 2 applications followed by exposure to drought
Source: Cytozyme Laboratories, Inc., USA
Nutriplant AG reduced drought
damage to leaves by 55%
La
bo
rato
ry R
esu
lts
0
20
40
60
80
100
1 hour 2 hours 4 hours
Dam
age
/ele
ctr
oly
te lea
ka
ge
(%
of to
tal e
lectr
oly
tes)
Control Nutriplant AG
55% decrease
37
Model plant: Tomato Treatment: 2 applications, then exposed to 116º F (47ºC) heat for 2 hours Source: Cytozyme Laboratories, Inc., USA
Nutriplant AG Reduces Damage Caused by High Temperatures
Control Treated
Nutriplant AG increased plant resistance to high temperatures
La
bo
rato
ry R
esu
lts
38
Model plant: Tomato Treatment: 2 applications, then exposed to 23º F (-5ºC) for 2 hours Source: Cytozyme Laboratories, Inc., USA
Nutriplant AG Reduces Damage Caused by Low Temperatures
Control Treated
Nutriplant AG increased
resistance to cold
La
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rato
ry R
esu
lts
39
Nutriplant AG reduced the severity of Spotted
Wilt virus infection in tomatoes by 76%
Nutriplant AG Reduces the Severity of Plant Disease
Source: University of Georgia, USA Fie
ld R
esu
lts
40
Nutriplant AG reduced the
severity of Fusarium stalk rot
infection in corn by 59%
Source: Agricultural Research Institute, West Germany
Nutriplant AG Reduces the Severity of Plant Disease
Fie
ld R
esu
lts
41
Summary Through increased photosynthesis, nutrient uptake,
antioxidant activity and up-regulation of genes involved in
plant response to stress Nutriplant AG
increases crop yield and quality
42
Nutriplant products reduce negative
effects of abiotic and biotic stress,
resulting in increased crop yields and
quality
43
When to apply
Nutriplant SD?
44
Nutriplant SD on Dryland Winter Wheat Production
Fie
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Year
Dryland Winter Wheat Yields
Control Nutriplant SD Difference Difference
(bu/acre) (bu/acre) (bu/acre) (%)
2004 25.0 28.4 3.4 13.6
2005* 15.7 26.0 10.3 65.7
2006* 10.6 17.2 6.6 62.5
Results of Nutriplant SD applied directly to the seeds
Nutriplant SD increased
dryland winter wheat by an
average of 43.7%
*Crop exposed to abiotic stress of low precipitation and extreme heat in 2006
The highest yield increases of over 60% were
observed under drought and heat stress conditions
65.7
62.5
45
Nutriplant SD on Irrigated Winter Wheat Production
Fie
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esu
lts
Year
Irrigated Winter Wheat Yields
Control Nutriplant SD Difference Difference
(bu/acre) (bu/acre) (bu/acre) (%)
2002 69.5 73.5 4.0 5.8
2003 93.5 100.4 6.9 7.4
2004 69.4 73.5 4.1 5.9
2006* 32.0 37.2 5.2 16.3
Results of Nutriplant SD applied directly to the seeds
Nutriplant SD increased
irrigated winter wheat by
an average of 8.8%
*Crop exposed to abiotic stress of extreme heat
The highest yield increase of 16.3% were
observed under heat stress conditions
16.3
46
Economic Benefits of Nutriplant SD
Crop Crop Yield
Increase/acre Economic Benefit
Net profit/acre
Corn 5.6 bu/acre $35.63/acre
Pinto Beans 195 lb/acre $63.33/acre
Sugar Beets 3.0 ton/acre $140.02/acre
Sunflower 147 lb/acre $48.54/acre
Winter Wheat Dryland 6.8 bu/acre $48.76/acre
Winter Wheat Irrigated 5.1 bu/acre $35.45/acre
Trials conducted 1998-2006 on six crops
47
Apply Nutriplant SD at planting
time to the following crops:
Corn, pinto beans, sugar beets,
sunflower, dryland and irrigated
winter wheat
48
When and how
to apply
Nutriplant SL?
49
Nutriplant SL on Corn Production
Fie
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esu
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Year
Grain Yields
Control Nutriplant SL Difference Difference
(bu/acre) (bu/acre) (bu/acre) (%)
2005 229 232 3 1.3
2006* 189 205 16 8.5
Year
Grain Yields
Control Nutriplant SL Difference Difference
(bu/acre) (bu/acre) (bu/acre) (%)
2008* 185 202 17 9.2
2010 204 204 0 0.0
2011* 191 204 13 6.8
Results of Nutriplant SL applied in furrow
Results of Nutriplant SL applied directly to the seeds
Both methods of application of
Nutriplant SL to corn seeds give
similar results
Nutriplant SL was most effective
during years when corn plants
were exposed to abiotic stress
increasing corn yields by an
average of 8.2% (15 bu/acre)
*Crop exposed to extreme abiotic stress of high winds and heat
50
Nutriplant SL on Soybean Production
Fie
ld R
esu
lts
Year
Soybean Yields
Control Nutriplant SL Difference Difference
(bu/acre) (bu/acre) (bu/acre) (%)
2005 62.7 65.8 3.1 4.9
2006 72.5 73.0 0.5 0.7
Year
Soybean Yields
Control Nutriplant SL Difference Difference
(bu/acre) (bu/acre) (bu/acre) (%)
2008* 57.4 61.8 4.4 7.7
2010* 39.6 49.5 9.9 25.0
2011* 71.0 77.7 6.7 9.4
Results of Nutriplant SL applied in-furrow
Results of Nutriplant SL applied directly to the seeds
The in-furrow application of
Nutriplant SL to soybeans
is more effective method
than direct application of
the product to the seeds
In a three year study
Nutriplant applied in-furrow
increased soybeans yields
by 14% (7 bu/acre)
*Crop exposed to extreme abiotic stress of high winds and heat in 2008
The highest yield increases
were observed under stress
conditions 25.0
9.4
51
Crop Crop Yield
Increase/acre Economic Benefit
Net profit/acre
Corn
in-furrow
on seed
10 bu/acre
10 bu/acre
$57.83/acre
$64.60/acre
Soybeans
in-furrow
on seed
7.0 bu/acre
Not recommended
$89.12/acre
Sugar Beets
in-furrow
on seed
3.5 ton/acre
5.47 ton/acre
$159.47/acre
$255.37/acre
Economic Benefits of Nutriplant SL
Trials conducted 2005-2011 on three crops
52
Apply Nutriplant SL at planting
time to the following crops: Corn (in-furrow or as seed coating)
Soybeans (in-furrow)
Sugar beets (in-furrow or as seed coating)
53
When to apply
Nutriplant AG?
54
Nutriplant AG on Soybeans
Fie
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Year
Soybean Yields
Control Nutriplant AG Difference Difference
(bu/acre) (bu/acre) (bu/acre) (%)
1999 52.1 53.1 1 1.9
2005 62.7 67.2 4.5 7.2
2008 54.0 58.8 4.8 8.9
Year
Soybean Yields
Control Nutriplant AG Difference Difference
(bu/acre) (bu/acre) (bu/acre) (%)
2010 39.6 53.8 14.2 35.9
2011 71.0 74.4 3.4 4.8
Results of Nutriplant AG applied at pod set
Results of Nutriplant AG applied at pre-bloom
Nutriplant AG applied at pre-
bloom increased soybean yield
by an average of 6%
Nutriplant AG applied at pod set
increased soybean yield by an
average of 20.4%
55
Nutriplant AG on Potato Production
Fie
ld R
esu
lts
Potato Yields
Control Nutriplant AG Difference Difference
(lb/acre) (lb/acre) (lb/acre) (%)
17,110a 23,250b 6,140 35.9
Nutriplant AG applied three times
during growing season increased
potato yield by 35.9%
Numbers followed by different letters are statistically significant (p<0.05)
Potato Yields
Control Nutriplant AG Difference Difference
(lb/acre) (lb/acre) (lb/acre) (%)
32,604 37,699 5,095 15.6
Nutriplant AG applied two times
during growing season increased
potato yield by 15.6%
Nutriplant AG applied three times during growing season
gives better potato yield than when applied two times
Effects of three applications of Nutriplant AG on Russet Burbank potatoes
Effects of two applications of Nutriplant AG on Yukon Gold potatoes
15.6
35.9
56
Nutriplant AG on Potato Tuber Size
Fie
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Potato Tuber Size Distribution (%)
Tuber Size Control Nutriplant AG
< 4 oz 83.3 76.9
> 4 oz 16.7 23.7
Effects of three applications of Nutriplant AG on Russet Burbank potatoes
Nutriplant AG applied three times
during growing season increased
percent of large tubers by 41.9%
Nutriplant AG applied two times
increased percent of large tubers
15.4% and medium tubers by 8.7%
Nutriplant AG increases the percent of large tubers
Potato Tuber Size Distribution (%)
Tuber Size Control Nutriplant AG
< 8 oz 41 35
8-14 oz 46 50
> 14 oz 13 15
Effects of two applications of Nutriplant AG on Yukon Gold potatoes
57
Fie
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Treatment
Grain Yield*
Yield Difference
(bu/acre) (bu/acre) (%)
Control 30.8 -- --
Nutriplant AG in spring at “green-up” 36.6 5.8 18.8
Nutriplant AG in spring at “green-up”
and again at “boot” stage
37.6 6.8 22.1
*Yield adjusted to 12% moisture and 60 lb/bu grain density
Nutriplant AG applied to
dryland winter wheat in
spring at “green-up”
produced 18.8% higher yield
Nutriplant AG on Dryland Winter Wheat Production
Nutriplant AG applied twice
at spring “green-up” and
again at “boot” stage
produced 22.1% higher yield
18.8
22.1
58
Crop Crop Yield
Increase/acre Economic Benefit
Net profit/acre
Corn 13.1 bu/acre $79.20/acre
Pinto Beans 763 lb/acre $246.32/acre
Potatoes
2 applications
3 applications
5,095 lb/acre
6,140 lb/acre
$552.28/acre
$661.93/acre
Soybeans 8.8 bu/acre $110.04/acre
Sugar Beets
1 application
2 applications
3.2 ton/acre
5.7 ton/acre
$142.44/acre
$252.19/acre
Economic Benefits of Nutriplant AG
Trials conducted 1998-2011 on five crops
59
Apply Nutriplant AG to the
following crops: Corn, pinto beans, potatoes, soybeans,
sugar beets and wheat
60
When to use
product
combinations?
61
Nutriplant SD and Nutriplant AG on Dryland Winter Wheat Production
Fie
ld R
esu
lts
Treatment
Grain Yield*
(bu/acre) Difference
(bu/acre) (%)
Control 33.3 -- --
Nutriplant SD with Nutriplant AG in fall at
“green-up”
38.8 5.5 16.5
Nutriplant SD with Nutriplant AG in
spring at “green-up”
41.7 8.4 25.2
Nutriplant SD with Nutriplant AG in fall
and again in spring at “green-up”
41.3 8.0 24.0
Nutriplant SD with Nutriplant AG at
spring “green-up” and again at “boot”
stage
40.8 7.5 22.5
Nutriplant AG applied to dryland
winter wheat in spring at “green-up” in
addition to Nutriplant SD application at
planting was the most effective
treatment resulting in 25% yield
increase
*Yield adjusted to 12% moisture and 60 lb/bu grain density
Second application of Nutriplant AG at
“boot” stage did not produce
additional yield increase of dryland
winter wheat
Application of Nutriplant AG in the fall
to dryland winter wheat is not as
effective as spring application
25.2
62
Fie
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esu
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Treatment
Grain Yield*
Yield Difference
(bu/acre) (bu/acre) (%)
Control 90.3 -- --
Nutriplant SD at planting 93.1 2.8 3.1
Nutriplant SD at planting
Nutriplant AG applied in the fall
94.2 3.9 4.3
Nutriplant SD at planting
Nutriplant AG applied in the spring
100.8 10.5 11.6
*Yield adjusted to 12% moisture and 60 lb/bu grain density
Nutriplant AG applied to irrigated
winter wheat in spring at “green-
up” in addition to Nutriplant SD
application at planting produced
the highest yield increase of 11.6%
Nutriplant SD and Nutriplant AG on Irrigated Winter Wheat Production
11.6
63
For best results, apply
product combinations to
all crops including:
Corn, pinto beans, potatoes,
soybeans, sugar beets and wheat
especially when stress conditions
are expected
64
Nutriplant SL applied with starter fertilizer
• Nutriplant SL in-furrow on corn
• Nutriplant SL in-furrow on soybeans
• Nutriplant SL in-furrow on sugarbeets
Combination of Nutriplant SL with AG
• Nutriplant SL in-furrow with AG on soybeans
• Nutriplant SL in-furrow and 1 application of
Nutriplant AG on sugarbeets
• Nutriplant SL in-furrow and 2 applications of
Nutriplant AG on sugarbeets
IRF Field Trials in 2012
65
Also Consider from 2011
• Nutriplant SL for transplants and drip irrigation of
perennial crops
• Nutriplant AG for transplants
• Nutriplant AG for green house applications
• Nutriplant products for turf applications
66
• Go to: www.amway.com
• Log in with username and password
• Search: Nutriplant
• Product pictures and labels
• Downloadable sales literature
• Nutriplant Fliers for 28 crops
• Call 1-800-992-6929 press 0 at anytime to talk
with a B to B agricultural product specialist
(Jim, Sandy or Patrick)
Resources from Amway®
67
Resources from Cytozyme®
• Go to: getproductsupport.net
• Fliers for Nutriplant SD/SL and for Nutriplant AG
• Fliers for 28 different crops
• Field results
• Testimonials
• Labels and pictures of Nutriplant products
• Technical support contact
68
How much did you sell in 2012?
Product 2011 2012 Jan.-Jul.
2012 Projected
Percent Increase
Nutriplant SD
25 lb bucket
2,802 2,747 4,709 68%
Nutriplant SL
1.6 gallon jug
2,286 1,096 1,879 18%
Reduction
Nutriplant AG
2.5 gallon jug
1,272 2,286 3,919 208%
Nutriplant AG
30 gallon drum
685 961 1,647 140%
Congratulations! 68% increase of Nutriplant SD
208% increase of Nutriplant AG in 2.5 gallon jug
140% increase of Nutriplant AG in 30 gallon
140%
208%
68%
69
Thank you for making 2012
another successful year!
70