Novel Physics arising from phase transitions in biology“In physics, we (try to) teach principles...
Transcript of Novel Physics arising from phase transitions in biology“In physics, we (try to) teach principles...
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Novel Physics arising from phase transitions in biology
IPLS RetreatBath, Oct 17, 2017
Chiu Fan LeeDepartment of Bioengineering, Imperial College London, UK
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Biology Physics
Biology inspires new physics
Physics leads to quantitative biology
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200nm
Amyloid fibrils RNA granules Active matter
Phase transitions in biology
https://www.youtube.com/watch?v=EimBzUSmak8
Brangwynne [Hyman Lab]
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Plan
1. Physics of polymer self-assembly
2. Non-equilibrium phase separation
3. Universality in active matter
4. Summary & Outlook
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1. Physics of polymer self-assembly
Slyngborg & Fojan (2015) Phys. Chem. Chem. Phys.
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Toy model
Liu, Lee & Huang, in Biophysics and biochemistry of protein aggregation (World Scientific, 2017)
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A colloidal example
Wang et al. (2012) Nature 491, 51
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Statistical mechanics
Energy of a 𝑠-mer:
Partition function (dilute limit):
Monomer number conservation:
Factorial because of indistinguishability by our choice (nothing to do with quantum mechanics!)
Liu, Lee & Huang, in Biophysics and biochemistry of protein aggregation (World Scientific, 2017)
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Static configuration
Solute concentration
Mas
s d
ensi
ty
Monomer
𝑙
𝑃(𝑙)
Critical fibrillar concentraion (CFC)
Length distribution
Monomer concentration ~ CFC
Cates & Candau (1990) J. Phys.: Condens. MatterLee (2009) Phys. Rev. E; Lee (2012) J. Phys.: Condens. Matter
NOT a thermodynamic phase transition
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Steady-State Kinetics𝐵𝑖−𝑗,𝑗
𝐴𝑖−𝑗,𝑗
𝜌𝑘: Concentration of 𝑘-mers
Thermal equilibrium:
𝐴𝑖𝑗 and 𝐵𝑖𝑗 are not independent!
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Breakage rate 𝐵𝑖𝑗
Over-damped EOM:
Breakage rate = (First-passage time)-1
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Asymptotic results
1D Kramers escape problem
Quasi-static approximation: 𝑃(𝜃) ∝ 𝑒−𝐵𝜃2
Exit rate:
Lee (2009) Phys. Rev. E; Lee (2015) J. Phys.: Condens. Matter
Mulitdimensional Kramers escape problem
Breakage rate of a s-mer by thermal bending:
Ener
gy U
’(θ
)
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Revisiting the kinetic equation
• Uniform breakage profile -> 𝐵𝑖𝑗 = 𝐵
• Steady state configuration
-> 𝐴𝑖𝑗 = 𝐴
• Namely, all joining rates of any pair of polymers are identical, irrespective of the sizes
• How does this square with the Smoluchowskipicture?
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Smoluchowski reaction kinetics
Hill (1983) Biophys. J.
Diffusion-controlled binary association rate:
where
Considering the rod-like nature as well:
What’s wrong with this picture?
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Breakage-controlled, NOT diffusion-controlled
We can compute the breakage rate from the Smoluchowskipicture and compare it to the true breakage rate
• Therefore, a polymer interacts predominately with its own fragments!
Lee (2017) Eprint: arXiv:1709.04684
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Spatial correlationDistribution of dimer’s center of mass
Lee (2017) Eprint: arXiv:1709.04684
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2. Non-equilibrium phase separations
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•No membrane
•Rapid turnover
•Coalescence
Formation by phase separation
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Phase diagram
𝜌
𝑇
Vapour Liquid
Phase separation region
𝜖 : supersaturation
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Drop growth
x
x
𝜌
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Drop growth
x
x
Vapour conc. outside small drop is higher than vapour conc. outside large drop
Gibbs-Thomson relation: 𝜌𝑅 = 𝜌∞ 1 +𝜈
𝑅
𝜌
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Universality of surface-tension driven droplet growth
• Lifshitz-Slyozov universal
growth law: 𝑅(𝑡) ~𝑡1/3
• Universal droplet size distribution
Ostwald coarsening
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Wurtz & Lee (2017) E-print: arXiv:1708.05697
Chemical reaction-controlled phase separation
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r
SG Cytoplasm
𝑅
SG Cytoplasm𝑅
Co
nce
ntr
atio
n
S
P
Chemical reactions create gradients
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Wurtz & Lee (2017) E-print: arXiv:1707.08433
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https://www.youtube.com/watch?v=v9xq_GiRXeE
3. Universality in active matter
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Incompressible fluids
c
Translational invarianceRotational invarianceGalilean invarianceFluctuation-dissipation
𝜕𝑡 𝑣 = −𝛻𝑃 + 𝑓 − 𝜆 𝑣 ∙ 𝛻 𝑣 − 𝑎 + 𝑏𝑣2 𝑣 − 𝜇𝛻2 𝑣 + 𝑐𝑣4 𝑣 + 𝜉 𝛻2 2 𝑣 + ⋯
𝜕𝑡 𝑣 = −𝛻𝑃 + 𝑓 − 𝑣 ∙ 𝛻 𝑣 − 𝜇𝛻2 𝑣
Symmetries + coarse-graining via renormalisation group transformation
Parameter-free prediction:
𝑣(𝑡) ∙ 𝑣(0) ∝ 𝑡− 𝑑/2
(Long-time tail)
Physical systems
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Incompressible active fluids
𝜕𝑡 𝑣 = −𝛻𝑃 + 𝑓 − 𝜆 𝑣 ∙ 𝛻 𝑣 − 𝑎 + 𝑏𝑣2 𝑣 − 𝜇𝛻2 𝑣 + 𝑐𝑣4 𝑣 + 𝜉 𝛻2 2 𝑣 + ⋯
𝜕𝑡 𝑣 = −𝛻𝑃 + 𝑓 − 𝜆 𝑣 ∙ 𝛻 𝑣 − 𝑎 + 𝑏𝑣2 𝑣 − 𝜇𝛻2 𝑣
Symmetries + coarse-grain via renormalisation group transformation
c
Translational invarianceRotational invarianceGalilean invarianceFluctuation-dissipation
Biological systems
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Critical order-disorder transition
Adapted from QuantumDiaries.org
Energy
x
y
The bottom has become very flat
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Phase diagram of incompressible active fluids
Critical region
𝑣
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Critical incompressible active fluids
Level of coarse-graining
RG transformation
EOM: 𝜕𝑡 𝑣 + 𝛻𝑃 − 𝑓 = −𝜆 𝑣 ∙ 𝛻 𝑣 − 𝑎 + 𝑏𝑣2 𝑣 − 𝜇𝛻2 𝑣 + 𝑐𝑣4 𝑣 + 𝜉(𝛻2)2 𝑣 + ⋯
𝜕𝑡 𝑣 + 𝛻𝑃 + 𝑓𝑙 = −𝜆𝑙 𝑣 ∙ 𝛻 𝑣 − 𝑎𝑙 + 𝑏𝑙𝑣2 𝑣 − 𝜇𝑙𝛻
2 𝑣
Exact hydrodynamic EOM with TWO coefficients governing the model’s scale-invariance properties:
𝑔1(𝑙) ~𝐷𝑙𝜆𝑙
2
𝜇𝑙3
, 𝑔2 𝑙 ~𝐷𝑙𝑏𝑙
𝜇𝑙2
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Advective term:
𝑔1 𝑣 ∙ 𝛻 𝑣
Incompressible active matter (Today)
“Ferromagnets with dipolar interactions”Aharony and Fisher (1973) Phys. Rev. Lett.
𝑔1 𝑙 ~𝐷𝑙𝜆𝑙
2
𝜇𝑙3
; 𝑔2 𝑙 ~𝐷𝑙𝑏𝑙
𝜇𝑙2
Chen, Toner, Lee (2015) New J. Phys. 17, 042002
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Incompressible active matter (Today)
“Randomly stirred fluids (Model B)”
Forster, Nelson & Stephen(1977) Phys. Rev. A
“Ferromagnets with dipolar interactions”Aharony and Fisher (1973) Phys. Rev. Lett.
𝑔1 𝑙 ~𝐷𝑙𝜆𝑙
2
𝜇𝑙3
; 𝑔2 𝑙 ~𝐷𝑙𝑏𝑙
𝜇𝑙2
Chen, Toner, Lee (2015) New J. Phys. 17, 042002
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𝑔1 𝑙 ~𝐷𝑙𝜆𝑙
2
𝜇𝑙3
; 𝑔2 𝑙 ~𝐷𝑙𝑏𝑙
𝜇𝑙2
Chen, Toner, Lee (2015) New J. Phys. 17, 042002
“Ferromagnets with dipolar interactions”Aharony and Fisher (1973) Phys. Rev. Lett.
“Randomly stirred fluids (Model B)”
Forster, Nelson & Stephen(1977) Phys. Rev. A
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𝑔1 𝑙 ~𝐷𝑙𝜆𝑙
2
𝜇𝑙3
; 𝑔2 𝑙 ~𝐷𝑙𝑏𝑙
𝜇𝑙2
Chen, Toner, Lee (2015) New J. Phys. 17, 042002
Incompressible active fluids
In 3D: 𝑣( 𝑟) ∙ 𝑣( 𝑟′) ~ 𝑟 − 𝑟′ −(𝟏.𝟑𝟓±0.08)
“Ferromagnets with dipolar interactions”Aharony and Fisher (1973) Phys. Rev. Lett.
“Randomly stirred fluids (Model B)”
Forster, Nelson & Stephen(1977) Phys. Rev. A
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Adapted from QuantumDiaries.org
Energy
x
y
Ordered phase (2D)
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Ordered incompressible active fluids
Orderedphase Critical region
• Universality is more than criticality!
• Universal behaviour expected in the symmetry-broken phase of a continuous symmetry
𝑣
Belitz, Kirkpatrick, Vojta (2005) Rev. Mod. Phys.
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Ordered phase of 2D incompressible active fluids
Level of coarse-graining
RG transformation
EOM: 𝜕𝑡 𝑣 + 𝛻𝑃 − 𝑓 = −𝜆 𝑣 ∙ 𝛻 𝑣 − 𝑎 + 𝑏𝑣2 𝑣 − 𝜇𝛻2 𝑣 + 𝑐𝑣4 𝑣 + 𝜉(𝛻2)2 𝑣 + ⋯
Ordered phase:
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Chen, Lee, Toner (2016) Nature Comm.
Active fluids in 2D
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M.C. Escher (1938) Day and Night
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Summary
1. Biopolymer self-assembly
– Breakage-controlled thermalisation kinetics
2. Chemically active phase separations
– Coarsening arrested by chemical reactions
3. Universality in active matter
– Symmetry-based categorisation of non-equilibrium systems
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William Bialek
“In physics, we (try to) teach principles and derive the predictions for particular examples. In biology, teaching proceeds (mostly) from example to example. Although physics has subfields, to a remarkable extent the physicscommunity clings to the romantic notion that Physics is one subject.” [Biophysics: Searching for Principles (2012)]
Outlook 1
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Outlook 2
Anthony Zee, Quantum Field Theory in a Nutshell(Princeton University Press, 2003)
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The Team
Thank you for your attention!
BenPartridge
Jean DavidWurtz
DavidNesbitt
AndreaCairoli
AliceSpenlauhauer
Amyloids• Vaux Group (Dunn School of
Pathology, Oxford)• Liu Hong (Tsinghua
University)
Active Matter• Leiming Chen (China
University of Mining and Technology
• John Toner (Oregon)