Notes 7.Dnafingerprinting
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Transcript of Notes 7.Dnafingerprinting
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Notes 7DNA Fingerprinting
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Key Questions
1. How is DNA evidence used to solve crimes?
2. How do scientists create DNA fingerprints?
3. Once created how do you read and compare DNA fingerprints?
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DNA - Deoxyribonucleic acid
DNA is the hereditary material found in all cells.Its is a polymer made up of billions of nucleotides. The combination of nucleotides makes different proteins.
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The Human Genome
Humans have 46 chromosomes which contain roughly 25,000 genes;1% of the genome.
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•
The location of a gene on a chromosome is called a
locus. Alleles are different forms
of the same gene.
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Chromosome structure
Coding Regions & Non-coding regions
Portions of the DNA that code
for proteins
Portions of the DNA that doesn’t code for proteins
STR & VNTR length polymorphismsHemoglobin, Skin Pigment
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The genome differs from person to person by .1%
DNA fingerprinting compares these non coding regions to determine if crime scene DNA matches suspect DNA.
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Steps for creating a fingerprint
1. Isolate/Clean/Amplify the DNA sample(s).
2. Cut up the DNA with restriction enzymes.
3. Put DNA fragments through Electrophesis.
4. Take a picture.
5. Compare.
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DNA Basics
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DNA fingerprinting for paternity testing
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Paternity Evidence
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Paternity Evidence
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CIS DNA Fingerprinting
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CIS DNA Fingerprinting