NOSCaMV35S - دانشگاه تهرانjournals.ut.ac.ir/article_19618_466a8395f71c5d149... ·...
Transcript of NOSCaMV35S - دانشگاه تهرانjournals.ut.ac.ir/article_19618_466a8395f71c5d149... ·...
gus
gus
*
(Gossypium hirsutum L.)
LBA4404pBI121gusnptII
CaMV35SNOSgus
T1PCRGUS
(T1)
gusA3A4A7
(T1)nptII
(T1)PCRgus
gus
*
E-mail:[email protected]
(
uid
Gh-rbcsGh-spgus
Gh-spGh-rbcs
pBI121gusnptII
gus
gus
GhTUB1
GhTUB1
gus
LBA4404pBI121gus
CaMV35SnptII
Nos
OD600
mg/1
gus
MSKNO3
MgCl2
B5
MSB5
MS
GUS
(PCR)
T- DNA pBI121
gusnptIIOligo
''
GUS 3'-ACGTCCTGTAGAGAAACCCCAA-5
GUS 5'-CCCGCTTCGAAACCAATGCC-3
NPTII 3'-GAACAAGATGGATTGCACGC-5
NPTII 5'-GAAGAACTCGTCAAGAAGGC-3
TaqDNA
1. Cefotaxime
) dNTP
MgCl2
X
PCR
gus
T0
(T1)
MS
pH=8
GUS
2
nptII
nptII
PCR
nptII
nptII
gus
gus
gus
gus
PCR
nptII
DNA
DNApBI121
DNA
DNA
gus
gus
GUS
gus
PCR
gus
gus
PCR
gus
DNA
DNA
DNA
DNApBI121
DNA
gus(T1)
T0gus
gus
gus
gus
gus
GUS+ GUS-
A1 44 A2 4 3 A3 52 A4 6 2 A5 8 0 A6 4 4 A7 5 2 A8 5 3
gus
A3A4A7gus
A1A6
nptII
(gus+)
GUS
gus nptII
gus
nptII
PCR
nptII
DNA
DNA
DNA
DNApBI121
DNADNA
DNA
gus
2,4-D
pBI121
LBA4404EHA105
EHA105LBA4404
LBA4404
EHA101EHA105
REFERENCES1. Banerjee, A. K., D.C. Agrawal, S.M. Nalawade & K.V.Krishnamurthy.2002. Transient expression of
Glucuronidase in embryo axes of cotton by Agrobacterium and particle bombardment. Biologia Plantarum 45(3): 359-365
2. Banerjee, A.K., D.C. Agrawal, S.M. Nalawade, S. Hazra, & K.V. Krishnamurthy. 2003. Multiple shoot induction and plant regeneration from embryo axes of six cultivars of Gossypium hirsitum. Biologia Plantarum 47(3):433-436
3. Brasileiro, A.C.M., & Aragao, F. J. L. Aragao. 2001. Marker genes for in vitro selection of transgenic plants. Plant Biotechnology 3(3): 113-121
4. Donaldson, P. A & D. H. Simmonds. 2000. Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean. Plant Cell Rep 19: 478-484
5. Ellis, M. H., A. A Millar, D. J. Llewelly, W. Jammes Peacock, & E. S. Dennis. 2000. Transgenic cotton (Gossypium hirsutum) over-expressing alcohol dehydrogenase shows increased ethanol fermentation but no increase in tolerance to oxygen deficiency, Aust. J. Plant Physiol 27(2). 1041-1050
6. Guo .X., C. Huang, S. Jin, S.Liang, Y. Nie & X. Zhang, 2007. Agrobacterium-mediated transformation of Cry1C, Cry2A and Cry9C genes into Gossypium hirsitum and plant regeneration. Biologica Planetarium 51(2): 242-248
7. Haq, I., & Y. Zafar. 2004. High frequency of callus induction. Its proliferation and somatic embryogenesis in cotton (Gossypium hirsutum). J. Plant Biotechnology 6(1):55-61
8. James, C.2006. Global Status of Commercialized Biotech/GM Crops. International Service for the Acquisition of Agri-biotech Applications, Ithaca, New York, USA. briefs 35.
9. Jefferson, R. A. 1987. Assaying chimeric genes in plants: the gus gene fusion system. Plant Mol Biol Rep 5: 387-405
10. Jin, S., X. Zhang, S. Liang & Y. Nie. 2005. Factors affecting transformation efficiency of embryogenic callus of upland cotton (Gossypium hirsutum) with Agrobacterium tumefaciens. Plant Cell, Tissue and Organ Culture 81(2):229-237
11. Khumar, S. P., Sharma, & D. Penter. 1998. A genetic approach to in vitro regeneration of non-regeneration cotton (Gossypium hirsutum L) cultivars. Plant Cell Reports 18: 59-63
12. Li, X. B., L. Cai, N. H Cheng & J.W. Liu. 2002. Molecular characterization of the cotton GhTUB1 gene that is preferentially expressed in fiber. Plant Physiol 130(2): 666-674
13. Mishra, R., H. U. Wang, N. R. Yadav & T. A. Wu. 2003. Development of a highly regeneration elite Acala cotton (Gossypium hirsutum cv. Maxa) a step towards genotype-independent regeneration. Plant Cell Tiss. Org.Cult 73(1): 21-35
14. Norma, L. N. L. Trolinder & C. Xhlxlan. 1989. Genotype specify of the somatic embryogenesis respone in cotton. Plant Cell Reports 8 : 133-136
15. Rajasekaran, K. J., W. Grula, R. L. Hudspeth, S. Pofelis & D. M. Anderson.1996. Herbicide-resistant Acala and Coker cotton transformed with a native gene encoding mutant forms of acetohydroxyacis synthase. Molecular Breeding 2: 307-319
16. Rajasekaran, K. R., L. Hudspeth, J. W. Cary, D. M. Anderson & T. E. Cleveland, 2000. High frequency stable transformation of cotton (Gossypium hirsutum L.) by particle bombardment of embryogenic cell suspension cultures, Plant Cell Reports, 19(4): 534-545.
17. Rashid, H., S. Yokoi, K. Toriyama & K. Hinata. 1996. Transgenic plant production mediated by Agrobacterium indica rice. Plant Cell Reporter 15: 727-730.
18. Tohidfar. M. & M. Kaviyani. 2005. Marker genes for invitro selection of transgenic plants. Modern Genetic. 5: 23-28.
19. Tohidfar, M., C. Abdimishani, M. Mohammadi, & B. Ghariyazie. 2000. Effects of genotype and growth medium on callus formation and regeneration of cotton seed and plant. 17: 376-385.
20. Satyavathi, V. V., V. Prasad, B. G. Lakshmi & G. S. Lakshmi. 2002. High effeciency transformation protocol for three Indian cotton varieties via Agrobacterium tumefaciens. Plant Sci 162(2): 215-223.
gus
21. Song, P.H., J. L. Heinen, T.H. Burns & R. D. Allen. 2000. Expression of two tissue-specific
promoters in transgenic cotton plants. The Journal of Cotton Science 4(2): 217-223. 22. Sunikumar, G. & K. Ratore. 2001. Transgenic cotton: factors influencing Agrobacterium-mediated
transformation and regeneration. Molecular Breeding 8(1): 7-52. 23. Tohidfar, M., M. Mohammadi, & B. Ghareyazie. 2005. Agrobacterium-mediated transformation of
cotton (Gossypium hirsitum) using a heterologous bean chitinase gene. Plant Cell Tissue and Organ Culture (1)83: 83-96.
24. Wu, J., X. Luo, H. Guo, J. Xiao & Y.Tian. 2006. Transgenic cotton, expressing amaranthus caudatus agglutinin, confers enhanced resistance to aphids. Plant Breeding. 125(4): 390-394.
25. Zapata, C. S., H. Park, K. El-Zik, & R. H. Smith. 1998. Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex. Theor Appl. Genet 98(2): (2)252-256.
26. Zhang, B. H., R. Feng, F. Liu, & Q. Wang. 2001. High frerequency somatic embryogenesis and plant regeneration of an elite Chinese cotton variety. Bot. Bull. Acad. Sin 42(3): 9-16.
27. Zhang, B.H., F.Liu, Z. H. Liu, H.M. Wang & C.B. Yao. 2001. Effects of kanamycin on tissue culture and somatic embryogenesis in cotton. Plant Growth Regu 33(2):137-149.