Nobel Prize in Physiology or Medicine - 1974
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Nobel Prize in Physiology or Medicine - 1974For “the structure and functional organization of the cell”
Christian de Duve George E. PaladeAlbert Claude
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Modern Cell Biology – Structure and FunctionA Membrane Bound Perspective
1938 - Electron microscope available
Albert Claude – Rockefeller Institute used EM for visualizing cells Invented differential centrifugation and went on to discover the endoplasmic reticulum
George Palade – Discovered ribosomes and determined that they carry out protein synthesis
Went on to show that vesicles travel from ER to golgi to outside of the cell
Christian de Duve – Used fractionation to isolate groups of enzymes discovered lysosome and perioxisome
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A WELL-KNOWN TECHNIQUE FOR USING 35S OR 3H TO STUDY PROTEIN MOVEMENT
WITHIN A CELL
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After 10 minutes, insulinmoves from ER to Golgi
Pulse of pancreatic B cells with 3H
After 45 minutes, the protein is found in the secretory granules
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Flourescence Recovery after photobleaching (FRAP)Flourescence loss in photobleaching (FLIP) pg 608 in your book
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Three main types of coated vesicles:
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Clathrin is also involved in receptor mediated endocytosis
http://www.abcam.com/
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BOTH Clathrin and COPI/II assembly requires GTPases
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