Nmr

Francisco J Fuster RISE Program Seminar Summary Magnetic resonance as a tool for structural studies of membrane proteins: In this investigation, EPR (electron paramagnetic resonance) was used for the spin labeling of membrane proteins in order to get a reading in terms of mobility of that protein. NO (nitric oxide) was used to remove the free electron in order to use it for labeling. When exposed to a magnetic field the electron was removed and added to sulfur in the protein sfh1p. This protein was used because the natural sec14p protein was too unstable at high concentrations. The sfh1p supposedly has similar characteristics and functions as the sec14p. The main function of the sec14p is for signal transduction, transport and organelle biological activity regulation. However, the sfh1p cycles the lipids of the membrane. After the EPR was done, then the NMR (nuclear magnetic resonance) was used to locate the spin labeled sulfur in the protein. The readings were determines by the position of the label on the protein that determined the mobility of the protein. The faster the mobility, then the more possible biological activities took place. This is a type of signal transduction. The experimentation was executed “In vitro” maintaining constant pH and temperature because these proteins may be easily denaturalized by any slight change in any of these factors.

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Francisco J Fuster

RISE Program

Seminar Summary

Magnetic resonance as a tool for structural studies of membrane proteins:

In this investigation, EPR (electron paramagnetic resonance) was used for the spin

labeling of membrane proteins in order to get a reading in terms of mobility of that

protein. NO (nitric oxide) was used to remove the free electron in order to use it for

labeling. When exposed to a magnetic field the electron was removed and added to

sulfur in the protein sfh1p. This protein was used because the natural sec14p protein

was too unstable at high concentrations. The sfh1p supposedly has similar

characteristics and functions as the sec14p. The main function of the sec14p is for

signal transduction, transport and organelle biological activity regulation. However, the

sfh1p cycles the lipids of the membrane. After the EPR was done, then the NMR

(nuclear magnetic resonance) was used to locate the spin labeled sulfur in the protein.

The readings were determines by the position of the label on the protein that

determined the mobility of the protein. The faster the mobility, then the more possible

biological activities took place. This is a type of signal transduction. The experimentation

was executed “In vitro” maintaining constant pH and temperature because these

proteins may be easily denaturalized by any slight change in any of these factors.

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