Nicholas lab: C. elegans researchWorking with wormsPostgraduate induction 2013Yee Lian Chew

C. elegans the roundwormSmall 1 mm long in adultsAnatomy has been fully mapped Highly stereotypicalReproduce sexually by mating with males and also by self-fertilisation (maintained as a haermaphrodite population)Short-lived Transparent

What we doMatthewMalloryPriyaIreneAnnaDuyguHannahSashiHongYee LianAaronEstelleSlavica

C. elegans techniques we useGeneration of transgenic lines biolistic transformation and microinjectionMolecular biology techniques including DNA/RNA work, qPCR, RNAiStaining, western blot, mass spec (iTRAQ)Microscopy EM, confocal, fluorescence, dissecting scopeDissections germline stainingAnd of course, lots of fun with worms

Worms are transparent and yes, they have brainsThey have 302 neurons

Co-localisation studies using transgenic C. eleganspdpy-7::HIS-24::mCherrypctbp-1::ctbp-1::gfpMerge Matthew Hoe

Any questions?Contact the boss: hannah.nicholas@sydney.edu.au