Next Generation Sequencing (NGS) Service Submission Form · Next Generation Sequencing (NGS)...
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New customers, write NEW in this field. Existing customers can find their LTM customer ID# in the header of result reports from previous submissions. Need help? Contact [email protected] . Total Number of Samples or Pools: Ship Date: To be completed at Charles River LTM Order Number: Receipt Number: Date Received: Next Generation Sequencing (NGS) Service Submission Form Date: Signed: To the best of my knowledge these samples and/or specimens do not contain any infectious agent or material which might pose a threat to human health. If this is not the case, please call Charles River before sending samples. Sample Information For more information on Microbiome testing, visit www.criver.com/microbiome . Page 1 of 2 Sample # Sample ID Age Sex Strain/Line Location Condition 1 Condition 2 1 2 3 4 5 6 7 8 9 10 Notes: Customer Information Same as Customer * = required Email* Phone* Zip Code* State* City* Address 2 Address 1* Institution* Contact* Bill To Information Email* Phone* Zip Code* State* City* Address 2 Address 1* Institution* Contact* Payment information required at the time of submission. PO # Pay by PO Pay by Credit Card (CC) Contact Phone To pay with a CC on file, indicate the last four (4) digits and expiration date. For CC security, do not write the full CC number. CC Last 4 Digits CC Exp Date LTM Customer ID#* Sample Type: Species: Project(s):
Transcript of Next Generation Sequencing (NGS) Service Submission Form · Next Generation Sequencing (NGS)...
New customers, write NEW in this field. Existing customers can find their LTM customer ID# in the header of result reports from previous submissions. Need help? Contact [email protected].
Total Number of Samples or Pools: Ship Date:
To be completed at Charles River
LTM Order Number: Receipt Number: Date Received:
Next Generation Sequencing (NGS) Service Submission Form
Date:Signed:
To the best of my knowledge these samples and/or specimens do not contain any infectious agent or material which might pose a threat to human health. If this is not the case, please call Charles River before sending samples.
Sample Information
For more information on Microbiome testing, visit www.criver.com/microbiome. Page 1 of 2
Sample# Sample ID Age Sex Strain/Line Location Condition 1 Condition 2
1
2
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10
Notes:
Customer Information Same as Customer* = required
Email*
Phone*
Zip Code*State*
City*
Address 2
Address 1*
Institution*
Contact*
Bill To Information
Email*
Phone*
Zip Code*State*
City*
Address 2
Address 1*
Institution*
Contact*
Payment information required at the time of submission.
PO #
Pay by PO
Pay by Credit Card (CC)Contact
Phone To pay with a CC on file, indicate the last four (4) digits and expiration date. For CC security, do not write the full CC number. CC Last 4 Digits
CC Exp Date
LTM Customer ID#*
Sample Type: Species:
Project(s):
Page 2 of 2
Shipping Instructions
Questions? Email [email protected] or visit www.criver.com/info/dx Next Generation Sequencing (NGS) is available for research purposes only. Fecal Sample Collection & Shipping Instructions For other sample types, please contact [email protected]. Charles River provides Next Generation Sequencing (NGS) of prokaryotic 16S ribosomal RNA (rRNA) in the microbiota of laboratory animals. Please note, it is important to follow aseptic technique when collecting samples, as contamination of samples with bacteria from the environment could confound experimental findings. Supplies • 2.0 mL (mouse) or 5.0 mL (rat) PCR microcentrifuge tubes (Safe-lock vials). • <= -20oC freezer for interim storage • Dry ice for shipping • Insulated shipping container • Shipping label • Zip-top bags Method 1. For every sample you intend to collect, record a unique ID, the species, and the number of fecal pellets per sample vial.
2. Use the same sample ID for paired sample types from the same animal(s). 3. Create vial labels with the sample ID and collection date. 4. Charles River-provided individually wrapped PCR microcentrifuge tubes are sterile; they can be disinfected and transferred
into an isolator according to your standard procedure. Alternatively, the unwrapped PCR tubes can be sterilized by autoclaving along with other isolator supplies.
5. Following aseptic technique, collect fecal pellets directly from the animals. 6. Freeze PCR microcentrifuge tubes within an hour of collection and keep frozen until shipment, as it is critical to preserve the relative abundance of
bacteria in the sample. 7. When shipping, schedule for next-day arrival. 8. For shipment, place sample tubes and the submission form in separate zip-top bags, in a labeled insulated shipping container with dry ice. Additional considerations for collection • Because the microbiota might vary according to the time of day, samples collected on different days should be collected at the same time. • Samples whose microbiota will be compared (e.g., treated vs. untreated) should be collected within an hour of each other. • Other key variables to consider include age, sex, and strain. Place a copy of page 1 of this form in the appropriate shipping container with your samples. Please ship following International Air Transport Association (IATA) guidelines; visit www.criver.com/shippingmaterials to request free sample collection and shipping materials online. Submit your samples to Charles River:
Charles River RADS 327 impasse du Domaine Rozier Domaine des Oncins 69210 SAINT GERMAIN NUELLES FRANCE
Charles River RADS Microbiome Laboratory 261 Ballardvale Street Building 22 Loading Dock Wilmington, MA 01887 USA
Please only print and include page 1 with your samples.
Want an easier way to fill out a submission form and track your testing with Charles River? Try using LTM™ -- the online order wizard will guide you through your testing request. Visit www.criver.com/ltm to get started.
Number of fecal pellets Species Min MaxMouse 3 10
Rat 1 3
