New Year’s Launch of Six New Technologies for Veterinary Clinical Pathology
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Transcript of New Year’s Launch of Six New Technologies for Veterinary Clinical Pathology
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New Year’s Launch of Six New Technologies for Veterinary
Clinical Pathology Hematology – Maureen McCullaugh
Blood Gas Analysis – Mick Garrett
Endocrinology – Joel Eluk, Penny Furney
Flow Cytometry – Inese Berzina, Amanda Lawler, Emma O’Neill
Multiplex Immunoassay – Marianna Serra, Penny Furney
Cellomics – Margarida Domingos
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Novel State-of-the-Art Biomarker Technologiesat UCD UVH
HematologyImmunology
Advia
ImmunochemistryCentaur
MSD
Guava
Cellomics
InCell
Blood Gas Analysis
Immunochemistry
RapidLab 1200
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Multiparameter Hematology System: ADVIA 2120
• Biggest advance in hematology technology for a generation in sensitivity, specificity and parameters added
Applicable to other body fluids eg effusions, BALF, CSF, & BM. Novel parameters eg reticulocyte size, maturity & Hb (most sensitive measure of Fe status), of platelet size & activation, of neutrophil myeloperoxidase, & of extracellular versus intracellular Hb• Managed by Maureen McCullaugh
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Sample Port: Automatic sampling with a single port for syringe and capillary samples. Complete with automatic clot detection and clearance, short sample and bubble detection. Can backflush to clear so don’t have to replace cartridge.
• Microsampler – as little as 30 uL
• Comprehensive analytic panel: blood gas (pH, pCO2, pO2); electrolytes (Na+, K+, Ca++, CI- ); Metabolic (glucose, lactate)
• Managed by Mick Garrett in out-of-hours / student lab
RapidLab 1200: Blood Gas Analyser
• latest model in market place
• delivers results for all parameters in 1 min
• maintenance-free, cartridge-based system
• LIMS interfaceable
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• Numerous Parameters• Thyroid T4, T3, TSH• Reproductive eg progesterone, estradiol, testosterone• Adrenal eg Cortisol (dog)• Cardiac eg troponin, CK-MB (dog, horse)• Vit B12 and folate
• Insulin• Skeletal Muscle troponin
• Managed by Joel Eluk and Penny Furney
Centaur CP• automated Immunoanalyzer
• latest model in market place
• replaces Immulite
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Guava – Personal Flow Cytometer
• smallest and easiest to use in marketplace
• 2-angle scatter, 4 color
• being developed for use for immunophenotyping of lymphoma and leukemia
• Assessment of cell viability
• Managed by Inese Berzina, Amanda Lawler, and Emma O’Neill
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• Managed by Marianna Serra, Penny Furney
MSD 2400• automated mulitplexing immunoanalyzer
• latest model in market place
• for high throughputting, needing microvolume testing (25 uL vs 150 uL), needing mutliplexing
• assay development possible• panels of mulitple cytokines and chemokines
• cardiac panel of biomarkers
• reproductive hormones
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♦ Automated microscopy ♦ 96-well plate in incubator♦ Live-cell, image analysis ♦ Single cell, populations, well l♦ Real-time, kinetic, multiplex ♦ Intracellular location♦ Structure & function ♦ Managed by Margarida Domingos
High Content Analysis & Hormesis