Summary ofNew Products Introduced:

September 9-13, 2013

For more detailed product info visit: https://www.caymanchem.com/NewProducts

600870 TAF1 bromodomain 1 TR-FRET Assay Kit600930 TAF1 bromodomains 1 & 2 TR-FRET Assay Kit

TAF1• a component of transcription factor IID• interacts with transcriptional activators to

promote transcription• Recognition of acetylated lysine

residues via its two bromodomains directs TAF1 binding to the core promoter sequences at the transcription start site

 • homogeneous TR-FRET assay method• rapid characterization of inhibitors of

bromodomain/acetylated peptide interaction

• amenable to high throughput format

 

robust, Z’ >0.6

384 wells $495 1920 wells $1,8569600 wells $8,313

700880 p-Aminohippuric Acid (PAH) Assay Kit

colorimetric method for detecting PAH from plasma and urine

used to measure effective renal plasma flow (eRPF)

At low doses, PAH is almost completely eliminated from the blood during a single pass through the kidney

also be used to measure net tubular organic anion secretion

Uptake of PAH from blood into the tubular cells occurs by exchange with intracellular α- ketoglutarate and is mediated by the organic anion transporter 1

 

96 wells $295

image credit: http://droualb.faculty.mjc.edu/Course%20Materials/Physiology%20101/Chapter%20Notes/Fall%202011/chapter_18%20Fall%202011.htm

700910 Catalase Assay Kit (without Hydrogen Peroxide)

this assay utilizes the peroxidatic function of CAT to determine enzyme activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates

Catalase (CAT) • ubiquitous antioxidant enzyme involved in the

detoxification of hydrogen peroxide (H2O2), a toxic product of both normal aerobic metabolism and pathogenic ROS production

• catalyzes the conversion of two molecules of H2O2 to molecular oxygen and two molecules of water (catalytic activity)

• demonstrates peroxidatic activity, in which low molecular weight alcohols can serve as electron donors

 

96 wells $270 480 wells $490

2H2O2 2H2O + O2 catalase

14170 MDC1 BRCT domains (human recombinant)

MDC1 • contains two BRCA1 C-terminal (BRCT) domains,

which recognize linear phosphoserine or phosphothreonine regions to mediate protein-protein and protein-DNA interactions

 

C-terminal BRCT domains of MDC1 recognize phosphorylated histone H2AX (γH2AX), a marker of DNA damage

binds to and retains the Mre11-Rad50-Nbs1 complex at sites of double-stranded breaks in DNA

serves as a scaffold for a DNA repair locus which attracts BRCA1 and 53BP1 to effect DNA repair by either non-homologous end-joining or homologous recombination

 

25 µg $125 50 µg $198 100 µg $374

14701 JARID1B/PLU1 (C-Term) Polyclonal Antibody

Antigen: JARID1B human protein (amino acids 1534-1544) · Host:rabbit · Application(s): FC and ICC · 

Jumonji AT-Rich Interactive Domain 1B

nuclear protein that is a potential histone demethylase

JARID1B demethylation of Lys-4 of histone H3 plays an important role in breast cancer cell proliferation through BRCA1 repression

also acts as a transcriptional co-repressor of two developmental transcription factors, PAX-9 and BF1, which are crucial for organogenesis

 

1 ea $229

14904 GPR120 (Internal) Polyclonal Antibody

Antigen: human GPR120 amino acids 247-258 (SEITKASRKRLT) · Host:rabbit · Application(s): ELISA and WB · 

A G protein-coupled receptor expressed in adipocytes and pro-inflammatory macrophages

activated by long chain free fatty acids

activation results in elevated Ca2+ and activation of the ERK cascade

ω-3 fatty acids initiate GPR120 signaling resulting in anti-inflammatory effects

indirectly involved with promoting insulin secretion

 

1 ea $229 Image credit: Cell, Volume 142, Issue 5, 687-698, 3 September 2010

HMG-CoA reductase inhibitors

10010334 Fluvastatin• (Ki = 0.3 nM)

• trade name Lescol® for the treatment of hypercholesterolemia in the prevention of cardiovascular disease

• reduced LDL cholesterol levels by 27% after six weeks of treatment with a dose of 40 mg/kg twice a day compared to placebo in clinical trial

10010342 Pravastatin• (Ki = 2.3 nM)

• a ring hydroxylated metabolite of mevastatin• marketed as Pravachol™ or Lipostat™ for reducing LDL

cholesterol and triglyceride levels and increasing HDL cholesterol in the prevention of cardiovascular disease

 

10 mg $29 25 mg $65 50 mg $109 100 mg $189

10 mg $29 25 mg $65 50 mg $109 100 mg $189

11878 Salicylcurcuminoid

synthetic curcuminoid

antioxidant and anticarcinogenic properties

increases the activity of quinone reductase in

mouse hepatoma cells in a dose-dependent

fashion

  1 mg $39 5 mg $176 10 mg $312

See also:81025                   Curcumin81025.1 Curcumin (technical grade)10960 Bisdemethoxycurcumin10961 Demethoxycurcumin10009986             Dimethoxycurcumin14451                    J 14711879                    2-HBA

Image credit: http://www.dallasnews.com/lifestyles/health-and-fitness/health/20110815-researchers-call-curcumin-solid-gold-for-its-curative-properties.ece

14749 Fostriecin (sodium salt)

inhibits serine/threonine protein phosphatases

(PP2A IC50 = 3.2 nM)

(PP4 IC50 = 3 nM)

increases the level of histone H3 phosphorylation and may alter epigenetic regulation of cell proliferation

first identified as an antitumor antibiotic

  10 µg $145

Image credit: Molecular Cell Volume 41, Issue 6, 18 March 2011, Pages 636–648

sPLA2 inhibitors

14902 Luffariellolide• reversibly inhibits sPLA2 isoforms from bee venom (IC50 = 230

nM) and snake venom, reducing inflammation

• blocks the production of PAF in stimulated neutrophils (IC50 = 5 μM)

• structural mimic of all-trans retinoic acid (RA) • In RA-sensitive cancer cell lines, induces the expression of RAR

target genes and inhibits cell growth

 

14903 Manoalide• irreversibly inhibits sPLA2 isoforms from human synovium, bee

venom, and cobra venom (IC50 = 3.9, 7.5, and 2 μM, respectively)

• potently blocks calcium channels (IC50 = 1 μM)

• inhibits phospholipase C (IC50 = 1.5 μM)

 

1 mg $120

1 mg $160

14915 Oxymatrine

natural alkaloid isolated from the root of S. flavescens, an herb used in traditional Chinese herbal medicine

antiviral properties = inhibits the replication of hepatitis B

prevents fibrosis in a number of organs including liver, by interfering with TGF-β signaling

induces apoptosis in certain cancer cells

ameliorates inflammation by impairing the

translocation of NF-κB

 

50 mg $39 100 mg $66 500 mg $137 1 g $195

Image credit: http://en.wikipedia.org/wiki/File:Sophora_flavescens.jpg

14916 Protocatechuic Acid

dihydroxybenzoic acid phenolic compound found in many edible and medicinal plants

major metabolite of antioxidant polyphenols found in green tea

free radical scavenging capability (IC50 = 16.3 μM)

anti-inflammatory, antihyperglycemic, neuroprotective, and anticancer activities

 

25 g $35 50 g $63 100 g $112

Receptor Antagonists

14914 NBQX (sodium salt)• AMPA- and kainate-sensitive glutamate receptor antagonist

• (IC50 = 0.15 and 4.8 µM, respectively)

• does not block the NMDA-sensitive glutamate receptor (IC50 > 90 µM)

 

14959 CTAP• selective µ opioid receptor antagonist (IC50 = 3.5 nM)

• 10-fold more potent than naltrexone as an antagonist of diverse compounds with antinociceptive effects through the µ opioid receptor

• resists enzymatic metabolism in the blood and passes the BBB

 

1 mg $45 10 mg $158 50 mg $675

1 mg $79

14962 Ryanodine

poisonous alkaloid found in the South American plant R. speciosa

prototypical ligand for ryanodine receptors, a family of calcium release channels located in skeletal and cardiac muscle

used as a potent organic insecticide specific against crop-damaging insects

investigative tool for identifying the various ryanodine receptor isoforms and characterizing their role in calcium signaling

 

1 mg $125

• nanomolar concentrations increases the calcium permeability of sarcoplasmic reticulum membranes leading to muscular contractions

• micromolar (~100 μM) concentrations irreversibly inhibit ryanodine receptor channel opening, which results in paralysis

Image credit: http://www.cs.stedwards.edu/chem/Chemistry/CHEM43/CHEM43/Ryanodine/RyRproject.html

14963 Tetrodotoxin

potent marine-derived neurotoxin that reversibly inhibits the inward sodium current through voltage-activated sodium (NaV) channels

blocks nerve and muscle action potentials

used in Hodgkin and Huxley’s classic experiments to elucidate the physical biology of nerve action potentials

indispensible neuroscience tool to pharmacologically dissect the contribution of the NaV current in excitable neurons

used to rationally design compounds for the treatment of neuropathic pain

 

14964 Tetrodotoxin Citrate = citrate-stabilized form designed better solubility

 

1 mg $135

1 mg $145

Image credit: http://commons.wikimedia.org/wiki/File:Poisson-lune.jpg

Dyes and Indicators

14919 XTT (sodium salt)• cell-impermeable, negatively charged tetrazolium dye that produces

a water-soluble formazan when reduced at the cell surface by cellular-derived NADH and an electron mediator

• frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis

14960 Fluo 3AM• acetoxymethyl (AM) ester of Fluo 3, a fluorescent calcium indicator

commonly used in flow cytometry and cell-based experiments to detect changes in intracellular levels

• cell permeable and readily taken up by living cells, whereupon the AM moiety is enzymatically removed to produce Fluo 3

 

25 mg $39 50 mg $62 100 mg $101 250 mg $215

500 µg $155 1 mg $248

New Forensic Products

 14292 ADB-FUBINACA

designer drug identified in synthetic cannabis blends in Japan in 2013

14897 N-methyl-2-AI (hydrochloride) novel aminoindane with potential for abuse

Deuterated Standards for RCS-4 (synthetic JWH 018 cannabinoid analog) metabolites:  14363 RCS-4 N-(4-hydroxypentyl) metabolite-d5

14364 RCS-4 N-(5-hydroxypentyl) metabolite-d5

14365 RCS-4 N-pentanoic acid metabolite-d5