New Breeding Technologies: Comparing Old and New Ways to Genetically Change Organisms
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Transcript of New Breeding Technologies: Comparing Old and New Ways to Genetically Change Organisms
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 1
New Breeding Technologies:Comparing Old and New Ways to Genetically Change Organisms
Adrianne Massey, PhD
Managing Director, Science and Regulatory Affairs
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 2
History of Crop Genetic Modification
Understanding the historical development of
crop genetic modification is essential for:
• understanding relative risks
• designing appropriate regulations (hopefully)
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 3
Genetic modification of food is not new
Humans have intentionally changed the genetic makeup of all
• Crops we grow
• Livestock we raise
• Microorganisms we use in food processing
“GMO” = all food, except wild fish/game and
wild fruit (e.g., berries)
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 4
History of Crop Genetic Modification
Crop domestication = Crop genetic modification. Began 10,000 years ago.
Selected seeds from certain plants to be planted for the next year’s crop.
Genetic modification through artificial selection.
[artificial (human) selection vs. natural selection]
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Teosinte – wild ancestor of maize
Modern Maize
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 6
Historical Development of Maize
Wild, weed relative
Evolution by Artificial Selection
Early humans changed teosinte into maize
by “selecting for” certain traits (genes) and
“selecting against” other traits (genes)
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 7
Historical Development of Maize
Of the 59,000 genes in maize, early humans focused on selecting for traits encoded by only 1700 genes.
What do those other 57,300 genes do? Who knows?????
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 8
History of Crop Genetic Modification
Stage 1. Artificial Selection – work with existing variation
Stage 2: Selective Breeding
Began when we learned how plants reproduce (1660’s)
Controlled which plants reproduced -
Shaped the variation in the population.
Then selected certain seeds for next year’s crop.
femalemale
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 9
Selective Breeding Within Same Species
At first……
Same SpeciesShared gene pool
Can exchange genes naturally through
sexual reproduction
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 10
Next Step: Selective breeding between different species
Same genusDifferent species
Same species
“Wide Crosses” that would not occur naturally
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 11
Selective Breeding Across Species
Some varieties of all major crops came from breeding different species with each other.
Corn Tomato Rice Oat Canola Wheat Soy Potato BarleyBeets Squash Cotton
First fertile, between-species cross in 1700’s“Not natural”
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Different genusDifferent species
Next Step: Selective Breeding between different genera
Same genusDifferent species
Same species
Different genusDifferent species
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 13
Selective Breeding Across Genera
Bread wheat has beencrossed with at least eleven different species in six different genera.
1890’s - first fertile between-genus cross
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 14
A Set of Lab Techniques Made “Wide Crosses” Possible
Bridge species
Chromosome doubling (chemical colchicine)
Embryo rescue
Treat with hormones, immunosuppressants
Protoplast fusion
Anther culture
Diploid tissue culture
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 15
“Natural” Plant Breeding (long before genetic engineering)
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 16
Genetic Modification through Mutagenesis
What if the existing genetic variation in accessible gene pools is limited?
Plant breeders create new genes in crop plants with mutagens, such as X-rays.
This form of genetic modification is mutagenesis breeding.
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 17
Genetic Modification through Mutagenesis
Since the 1930s, plant breeders have used mutagenesis to create new genes in more than 2700 crop varieties that were introduced to the food supply.
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 18
Genetic Engineering
The next step in the continuum of genetic modification techniques.
Most like selective breeding because it uses existing genetic variation.
“ Recombinant DNA technology ” - rDNA
Genetic engineering Transgenic crops
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Each dot - thousands of genesExcept single gene - disease resistance.
Thousands of genes withUnknown functions
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new variety
One gene Known function Inserted into familiar crop variety.
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“Genetically Modified” Crops Genetic modification technological continuum
• Selective Breeding -within same species (8000 BC) - between different species* (1700s) - between different genera* (1890s)
• Mutagenesis Breeding* (1930s)
• Genetic Engineering* (1983)
* - “unnatural”
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 22
Continuum of technological change characterized by
Improved precision and predictability
Increased dependence on scientific understanding
History of Genetic Modification
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 23
Modification Precision Continuum
Selective Breeding thousands of genes unknown function
Mutagenesis totally random unknown number of genes unknown function
Genetic Engineering 1-2 genes
known function
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 24
New Plant Breeding Technologies
The trend continues ………..
Increased dependence on scientific understanding
Improved precision and predictability
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 25
New Plant Breeding Technologies
Examples:• Zinc finger nucleases - ZFN (3 types)• Oligonucleotide directed mutagenesis (ODM)• Induced DNA methylation
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New Plant Breeding Technologies (NBTs)
Past: Random insertion of new genecisgene (same species) or transgene
Now: Targeted gene insertion (ZNF -3)
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 27
New Plant Breeding Technologies
Zinc finger nucleases – ZFN-1 and ZFN-2Oligonucleotide directed mutagenesis (ODM)
These technologies allow very precise editing of plant’s existing genetic material (genome) • Single gene deletion• Single nucleotide changes
New genes are not added to crop
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 28
New Plant Breeding Technologies
Induced DNA methylation
• No change in genome/gene• Change in gene expression
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 29
Regulation of NBTs
What does this mean for regulation?
Increased scientific understanding
Improved precision and predictability
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Breeding ?
or
GE?
Genetic Improvement of Crops
Thousands of genes unknown function
Single gene ofknown function
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Breeding
GE
Costs of Regulatory Compliance
$ 0
$15-36 million
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 32
History of Crop Genetic Modification
Understanding the historical development of
crop genetic modification is essential for:
• understanding relative risks
Seems irrelevant for
• designing appropriate regulations
As we learn more about the molecular biology
of plants, the regulatory system becomes
increasingly burdensome.
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 33
1980’s Scientific Consensus on Risks
Risk GE crops = Risks GM crops
Product vs Process Risk Assessment - National Academy of Sciences
- OECD Expert Panel
- Ecological Society America
- American Society Microbiology
- American Medical Association
- Office Technology Assessment (US Congress)
- Environmental Defense Fund
- Audubon Society
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 34
Risk and Regulation
Regulatory policy is shaped by:• Science-based risk• Public perception of risk
Science-Based Risk Assessment
“Public” Perception of Risk
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BIOTECHNOLOGY INDUSTRY ORGANIZATION 35
Sample of Scientific Community Support
American Council on Science and HealthAmerican Dietetic AssociationAmerican Institute of Biological ScienceAmerican Medical Assoc. Council on Scientific AffairsAmerican Phytopathological SocietyAmerican Society of AgronomyAmerican Society for Cell BiologyAmerican Society for Horticulture scienceAmerican Society for MicrobiologyAmerican Society of Plant BiologistsAmerican Society of Plant PhysiologistsBrazilian Academy of SciencesChinese Academy of SciencesCouncil for AgriScience and TechnologyCrop Science Society of AmericaEntomological Society of AmericaFederation of Animal Scientific SocietiesFood and Agriculture Organization
Genetics Society of AmericaIndian National Science AcademyInstitute of Food Science and TechnologyInstitute of Food TechnologistsInternational Academy of SciencesInternational Society of African ScientistsMexican Academy of Sciences National Academy of Science &
Technology of the PhilippinesNational Academy of Sciences of USANew Zealand Royal CommissionSociety of NematologistsSociety In Vitro BiologyPontifical Academy of SciencesThe Royal Society of LondonThird World Academy of SciencesWeed Society of America
New Plant Breeding Technologies
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Regulation: A Primer by Dudley and Brito
Number of pages/year in the U.S. Code of Federal Regulations
Executive Orders Callingfor Regulatory Reform
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Regulation: A Primer by Dudley and Brito
Health and Environment Regulations
Economic Regulations
USA