Neurotropic - Ministerio para la Transición Ecológica y ...
Transcript of Neurotropic - Ministerio para la Transición Ecológica y ...
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Multitropic
Ebola
Enterotropic
Norovirus
Sapovirus
Rotavirus
Astrovirus
Adenovirus
Aichivirus
Hepatotropic
Hepatitis A
Hepatitis E
Neurotropic
Enterovirus
Poliovirus
Parechovirus
Nipah virus
TBE Pneumotropic
Influenza
SARS CoV-1
SARS-CoV-2
MERS-CoV
> 10.000.000
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Christian Drosten (La Charité, Berlin):
The proportion of infectious SARS-CoV-2 in
respiratory secretions is very low (around 1
infectious unit in 107 physical particles) and even
less in feces
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SARS-CoV-2 rapidly lose infectivity in the human GI tract.
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B) Vero E6 cells infected with SARS-CoV-2 isolate for 72 hours. C) Detection of viral particles by
using transmission electron microscopy (original magnification, ×98,000).
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Wu et al., 2020 The Lancet, https://doi.org/10.1016/S2468-1253(20)30083-2
Timeline of results from throat swabs and faecal samples through the course of disease
Prolonged Presence of SARS-CoV-2 Viral RNA in Faecal Samples
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• Medema G, Heijnen L, Elsinga G, Italiaander R, Brouwer A. Presence of SARS-
Coronavirus-2 in sewage. medRxiv. 2020.03.29.20045880
• Lodder W, de Roda Husman AM. SARS-CoV-2 in wastewater: potential health risk, but
also data source. The Lancet Gastroenterology & Hepatology. 2020;5(6):533-4.
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• EDAR Besòs: 3 M inhabitant equivalents
• EDAR El Prat de Llobregat: 2 M inhabitant equivalents
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Primer regions:
RdRp: IP2, IP4, Institut Pasteur
Envelope protein: E, Charité Berlin
Nucleoprotein: N1, N2, CDC
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Primer muestreo: 31 de marzo
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Primer muestreo: 31 de marzo
Primer positivo: 15 de enero
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Primer positivo: 15 de enero
Primer caso COVID-19
confirmado: 25 de febrero
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Entrada Fase 1: 25 de Mayo
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SARS-CoV-2 in Barcelona sewers – End of May 2020
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IP2
IP4
SARS-CoV-2 in raw sewage
samples from March 2019
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IP2
IP4
SARS-CoV-2 in raw sewage
samples from March 2019
Enterovirus
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Average standard curves for each of the targets used in this study (IP2, IP4, E, N1 and N2). A. Regression line (solid lines) and their 99% confident (dashed lines). B. Parameters defining each of the curves.
Genome copies
10-1
100
101
102
103
104
105
Cq
valu
es
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
10-1
100
101
102
103
104
105
10-1
100
101
102
103
104
105
10-1
100
101
102
103
104
105
10-1
100
101
102
103
104
105
IP2 IP4 E N1 N2
TARGET*SLOPE INTERCEPT EFFICIENCY
R2
Percent Replicates
Positivity
101 Genome
copies
100 Genome
copies
IP2 -3.421 40.228 96.0 0.981 33 33
IP4 -3.404 39.376 96.7 0.992 67 28
E -3.436 39.152 95.5 0.994 100 50
N1 -3.513 39.543 92.6 0.995 100 0
N2 -3.120 38.540 109.2 0.991 100 67
A
B
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Average standard curves for each of the targets used in this study (IP2, IP4, E, N1 and N2). A. Regression line (solid lines) and their 99% confident (dashed lines). B. Parameters defining each of the curves.
Genome copies
10-1
100
101
102
103
104
105
Cq
va
lue
s
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
10-1
100
101
102
103
104
105
10-1
100
101
102
103
104
105
10-1
100
101
102
103
104
105
10-1
100
101
102
103
104
105
IP2 IP4 E N1 N2
TARGET*SLOPE INTERCEPT EFFICIENCY
R2
Percent Replicates
Positivity
101 Genome
copies
100 Genome
copies
IP2 -3.421 40.228 96.0 0.981 33 33
IP4 -3.404 39.376 96.7 0.992 67 28
E -3.436 39.152 95.5 0.994 100 50
N1 -3.513 39.543 92.6 0.995 100 0
N2 -3.120 38.540 109.2 0.991 100 67
A
B
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Sample
0 1 2 3 4 5 6 7
Cq
Va
lue
s
25
30
35
40
45
50
IP2
IP4
E
N1
N2
Levels of SARS-CoV-2 genomes (Cq Values) in 6 sewage samplesemploying the fivetargets (IP2, IP4, E, N1 and N2)
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CoV Genome Organization and Expression
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CoV Genome Organization and Expression
IP4 E
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La vigilancia del SARS-CoV-2 en aguas residuales es una herramienta de alerta rápida para la COVID-19
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Análisis SARS-CoV-2 en aguas residuales