needs Discover - static.fishersci.eu · Discover superior nucleic acid purification Applications...
Transcript of needs Discover - static.fishersci.eu · Discover superior nucleic acid purification Applications...
Discoversuperior nucleic acid purification
Applications Products to use Cat. #
PCR clean-up
Removal of nucleotides
GeneJET PCR Purification Kit K0701/2
FastAP Thermosensitive Alkaline Phosphatase
EF0651/2/4
Removal of primers
GeneJET PCR Purification Kit K0701/2
Exonuclease I EN0581/2
Silica Bead DNA Gel Extraction Kit K0513
Extraction of DNA from agarose gel
Standard agarose gel
GeneJET Gel Extraction Kit K0691/2
Silica Bead DNA Gel Extraction Kit K0513
TopVision Agarose R0491/2
Low melting point agarose gel
Silica Bead DNA Gel Extraction Kit K0513
GeneJET Gel Extraction Kit K0691/2
Agarase EO0461
TopVisionTM Low Melting Point Agarose
R0801
Removal of RNA from DNA preparations
RNase A, DNase and protease-free EN0531
RNase T1 EN0541/2
RNase A/T1 Mix EN0551
RNase I EN0601/2
Removal of DNA from RNA preparations
DNase I, RNase-free EN0521/3/5
Removal of proteins from DNA and RNA preparations
Proteinase K (recombinant), PCR grade
EO0491/2
DNA and RNA precipitation
Glycogen, RNA grade R0551
Glycogen, molecular biology grade R0561
3 M Sodium Acetate Solution, pH 5.2
R1181
* available in EU countries only
for all your molecular biology needs
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Applications Products to use Cat. #
Isolation of plasmid DNA
GeneJETTM Plasmid Miniprep Kit K0502/3GeneJET Plasmid Midiprep Kit K0481/2GeneJET Plasmid Maxiprep Kit K0491/2MagJETTM Plasmid DNA Kit K2791/2
Isolation of RNA
GeneJET RNA Purification Kit K0731/2GeneJET Whole Blood RNA Purification Mini Kit
K0761
GeneJET Plant RNA Purification Mini Kit
K0801/2
MagJET RNA Kit K2731/2MagJET Whole Blood RNA Kit K2751/2MagJET Plant RNA Kit K2771/2
Isolation of genomic DNA
GeneJET Genomic DNA Purification Kit
K0721/2
Genomic DNA Purification Kit K0512GeneJET Whole Blood Genomic DNA Purification Mini Kit
K0781/2
GeneJET Plant Genomic DNA Purification Mini Kit
K0791/2
Proteinase K (recombinant), PCR grade
EO0491/2
MagJET Genomic DNA Kit K2721/2MagJET Whole Blood Genomic DNA Kit
K2741/2
MagJET Plant Genomic DNA Kit K2761/2
Isolation of viral DNA and RNA
GeneJET Viral DNA and RNA Purification Kit
K0821
Viral DNA/RNA Purification Kit, (IVD)* DK0011MagJET Viral DNA and RNA Kit K2781/2
Thermo ScientificTM manual and automated nucleic acid purification solutions provide high quality DNA and RNA that is ready to use in routine or very demanding downstream applications.
• Find your solution and request your free sample at thermoscientific.com/yieldpurityvaluefis
Thermo Scientific GeneJET Nucleic Acid Purification Kits• Trusted silica membrane technology in the form of a convenient spin column• Superior purity and yields compared to other kits on the market• Extensively tested on a wide variety of sample types and include specialized protocols
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Plasmid DNA purification
DNA,
µg
250
200
150
100
50
0Thermo
Scientificvendor A vendor B vendor C
1st elution
2nd elution
vendor D
DNA,
ng/
µg
900
700
500
300
1000
Thermo Scientific
vendor A vendor B vendor C
1st elution
2nd elution
vendor D
200
400
600
800
Yields of high-copy plasmid DNA obtained using Thermo ScientificTM GeneJETTM Plasmid Midiprep Kit and other kits for midscale plasmid DNA purification at maximal recommended bacterial cell culture volumes.
Concentrations of eluted DNA obtained after the purification of high-copy plasmid DNA using different kits for midscale plasmid DNA purification at maximal recommended bacterial cell culture volumes.
Genomic DNA purification
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M
Genomic DNA from different plant material samples purified using the Thermo ScientificTM GeneJETTM Plant Genomic DNA Purification Mini Kit.1- Arabidopsis, 2- Tobacco leaves, 3- Tomato leaves, 4- Spinach leaves, 5- Wheat leaves, 6- Grape leaves, 7- Onion leaves, 8- Rice leaves, 9- Corn seeds, 10- Sunflower stalk, 11- Corn roots, 12- Beef roots, 13- Cucumber fruit, 14 - Sugar cane.
Total RNA purification
Total RNA purified from 50 mg of tomato leaves using the Thermo ScientificTM GeneJETTM Plant RNA Purification Kit and kits from other vendors. M- Thermo Scientific™ RiboRuler™ High Range RNA Ladder. 1- Thermo ScientificTM product, 2-5 other vendors.
Thermo Scientific MagJET Nucleic Acid Purification Kits • Compatible with a wide variety of sample
types, including cells and tissues, whole blood, plants, serum, plasma, and bacterial culture
• Protocols for automated and manual workflows provided
• Nucleic acid isolation via magnetic beads
M 1 2 3 4 5 6
Total RNA purified from various sources using the Thermo ScientificTM GeneJETTM RNA Purification Kit. M- RiboRuler High Range RNA Ladder (#SM1821) 1- mouse liver, 2- mouse spleen, 3- HeLa cells, 4- Cos-7 cells, 5- Saccharomyces cerevisiae, 6- Escherichia coli DH5α.
M 1 2 3 4 5
“Surprisingly, the cheapest kit we tested turned out
to have the best performance. It has the highest
yield on each of the tested plasmids. The purity of
the GeneJET elutions are also appreciable, closely
following that of the QIAprep kit. Finally, its buffers,
like the innuPrep kit’s, are named instead of coded,
which makes using the kit very easy and intuitive ...
we conclude that the GeneJET kit, of all those we
compared, delivers the biggest bang for its buck.”
-iGEM team member
Manual and automated workflows were used to purify RNA from N.tobacum leaf samples (each 50 mg) with the Thermo Scientific™ MagJET™ Plant RNA Kit. The Thermo Scientific™ KingFisher™ protocol was used for the automated workflow. Isolated RNA was analyzed by agarose gel electrophoresis (1%), M – Thermo Scientific™ RiboRuler ™ High Range RNA Ladder (Cat #SM1823).
Austria: +43 (0) 800 20 88 40 Belgique/België: +32 (0) 056 260 260 Denmark: +45 070 27 99 20 España: +34 902 239 303 Finland: +358 (0) 9 8027 6280 France: +33 (0) 3 88 67 53 20 Germany: +49 (0) 180 5 258221 Ireland: +353 (0) 1 885 5854 Italia: +39 02 953 28 258 Netherlands: +31 (0) 20 487 70 00 Norway: +47 (0) 22 95 59 59 Portugal: +351 21 425 33 50/4 Suisse/Switzerland: +41 (0) 56 618 41 11 Sweden: +46 (0) 31 352 32 00 UK: +44 (0)1509 23 11 66M
B134
0CCG
• Find your solution and request your free sample at thermoscientific.com/yieldpurityvaluefis